Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,810,964 |
G→A |
63.0% |
G594G (GGC→GGT) |
uvrA ← |
ATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,810,964 | 0 | G | A | 63.0%
| 27.1
/ 32.2
| 54 | G594G (GGC→GGT) | uvrA | ATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC |
| Reads supporting (aligned to +/- strand): ref base G (12/8); new base A (20/14); total (32/22) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01 |
AGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGC > minE/2810902‑2811029
|
aGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCt < 1:3356071/68‑1 (MQ=255)
aGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCt < 1:1923905/68‑1 (MQ=255)
cTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGcc > 1:2936369/1‑69 (MQ=255)
cTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGcc > 1:2505904/1‑69 (MQ=255)
cTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGcc > 1:3294641/1‑69 (MQ=255)
cTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGcc > 1:2015231/1‑69 (MQ=255)
cTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGcc > 1:517587/1‑69 (MQ=255)
cTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACCTCAATCTTGCGTTTGCCGCTCATGTACTGcc > 1:36573/1‑69 (MQ=255)
ttttCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGccc > 1:20837/1‑68 (MQ=255)
ttttCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCg > 1:3210032/1‑69 (MQ=255)
ttttCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCg > 1:1669718/1‑69 (MQ=255)
ttttCCAGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCg > 1:442122/1‑69 (MQ=255)
ttCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGAc < 1:2222149/69‑1 (MQ=255)
ttCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGAc < 1:2289775/69‑1 (MQ=255)
cGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGt > 1:1813050/1‑60 (MQ=255)
cGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGAc > 1:1866302/1‑67 (MQ=255)
cGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACt > 1:626395/1‑68 (MQ=255)
cGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACt > 1:3014532/1‑68 (MQ=255)
cGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACt > 1:626741/1‑68 (MQ=255)
cGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACt > 1:328551/1‑68 (MQ=255)
cGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACt > 1:3211667/1‑68 (MQ=255)
cGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACt > 1:3187332/1‑68 (MQ=255)
cGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACt > 1:309366/1‑68 (MQ=255)
cGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACt > 1:3023610/1‑68 (MQ=255)
cGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACt > 1:935953/1‑68 (MQ=255)
cGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACt > 1:2745165/1‑68 (MQ=255)
cGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACt > 1:1618300/1‑68 (MQ=255)
cGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACt > 1:170079/1‑68 (MQ=255)
cGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACt > 1:1837961/1‑68 (MQ=255)
cGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACt > 1:2153196/1‑68 (MQ=255)
cGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACt > 1:2334716/1‑68 (MQ=255)
cGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACt > 1:2348782/1‑68 (MQ=255)
cGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACt > 1:2490292/1‑68 (MQ=255)
cGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACt > 1:2633303/1‑68 (MQ=255)
ttcttcGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACTCCGGCAcc < 1:3158556/63‑1 (MQ=255)
ttcttcGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACTCCGGCAcc < 1:2926608/63‑1 (MQ=255)
ttcttcGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACTCCGGCAcc < 1:639900/63‑1 (MQ=255)
ttcttcGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACTCCGGCAcc < 1:2699019/63‑1 (MQ=255)
ttcttcGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACTCCGGCAcc < 1:596259/63‑1 (MQ=255)
ttcttcGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACTCCGGCAcc < 1:595102/63‑1 (MQ=255)
ttcttcGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACTCCGGCAcc < 1:1872706/63‑1 (MQ=255)
ttcttcGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACTCCGGCAcc < 1:2044899/63‑1 (MQ=255)
ttcttcGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACTCCGGCAcc < 1:344988/63‑1 (MQ=255)
ttcttcGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACTCCGGCAcc < 1:3289077/63‑1 (MQ=255)
ttcttcGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACTCCGGCAcc < 1:3140062/63‑1 (MQ=255)
tcttcGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACTCCGGCAcc < 1:1278469/62‑1 (MQ=255)
tcttcGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACTCCGGCAcc < 1:900880/62‑1 (MQ=255)
tcttcGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTCAACGACTCCGGCAcc < 1:927466/62‑1 (MQ=255)
tCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCgg < 1:2086104/47‑1 (MQ=255)
tCAATCTTGCGTTGGCCGCTCATGTACTGCCCGGTCAACGACTCCgg < 1:3306774/47‑1 (MQ=255)
aTCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCgg < 1:3354421/68‑1 (MQ=255)
cTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTc > 1:814356/1‑39 (MQ=255)
cTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGAc > 1:1936213/1‑68 (MQ=255)
tttGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGc < 1:2576438/69‑1 (MQ=255)
|
AGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGC > minE/2810902‑2811029
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
