Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,836,489 |
G→C |
67.5% |
A277A (GCC→GCG) |
yjeK ← |
predicted lysine aminomutase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,836,489 | 0 | G | C | 67.5%
| 139.2
/ 97.4
| 129 | A277A (GCC→GCG) | yjeK | predicted lysine aminomutase |
| Reads supporting (aligned to +/- strand): ref base G (37/5); new base C (33/54); total (70/59) |
| Fisher's exact test for biased strand distribution p-value = 5.11e-08 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 |
Only 100 of 137 total aligned reads displayed.
CACTCACCATAAAATGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGT > minE/2836422‑2836549
|
cactcacCATAAAATGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCgg < 1:31688/69‑1 (MQ=255)
cactcacCATAAAATGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCgg < 1:2958768/69‑1 (MQ=255)
cactcacCATAAAATGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCgg < 1:287414/69‑1 (MQ=255)
cactcacCATAAAATGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCgg < 1:283496/69‑1 (MQ=255)
cactcacCATAAAATGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCgg < 1:1226007/69‑1 (MQ=255)
cactcacCATAAAATGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCgg < 1:2430639/69‑1 (MQ=255)
cactcacCATAAAATGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCgg < 1:1671870/69‑1 (MQ=255)
cactcacCATAAAATGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCgg < 1:1570523/69‑1 (MQ=255)
aTAAAATGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGaac < 1:3265966/69‑1 (MQ=255)
tAAAATGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGaac < 1:3002456/68‑1 (MQ=255)
aaaTGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGaacaa > 1:599726/1‑68 (MQ=255)
aaaTGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGaacaa > 1:2038114/1‑68 (MQ=255)
aaaTGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGaacaa > 1:749700/1‑68 (MQ=255)
aaaTGCGCCGCGCCCTGTACTTTATCGAGAACATGCAGGTAATACGGCATTACGCCGGCATCGaac > 1:1019044/1‑66 (MQ=255)
tACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAgg > 1:620/1‑64 (MQ=255)
tACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGttt > 1:154598/1‑67 (MQ=255)
tACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGttt > 1:48062/1‑67 (MQ=255)
tACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGttt > 1:1597181/1‑67 (MQ=255)
cTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCa > 1:602290/1‑69 (MQ=255)
cTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCa > 1:798947/1‑69 (MQ=255)
cTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCa > 1:1819382/1‑69 (MQ=255)
cTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCa > 1:1706561/1‑69 (MQ=255)
cGAGCACATGCAGGTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGttt > 1:2264688/1‑69 (MQ=255)
cGAGCACATGCAGGTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGttt > 1:2235359/1‑69 (MQ=255)
cGAGCACATGCAGGTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGttt > 1:3365470/1‑69 (MQ=255)
cGAGCACATGCAGGTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGttt > 1:3087186/1‑69 (MQ=255)
cGAGCACATGCAGGTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGttt > 1:205650/1‑69 (MQ=255)
cGAGCACATGCAGGTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGttt > 1:1835633/1‑69 (MQ=255)
cGAGCACATGCAGGTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGttt > 1:3330262/1‑69 (MQ=255)
aTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGtt > 1:2439921/1‑69 (MQ=255)
aTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGtt > 1:8713/1‑69 (MQ=255)
aTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGtt > 1:1311767/1‑69 (MQ=255)
aTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGtt > 1:2969048/1‑69 (MQ=255)
aTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGtt > 1:1690016/1‑69 (MQ=255)
aTGCAGGTAATACGGCATTACGCCGGCATCGAACAAAGCATTACTCAGGTTTGCCAGCGTTTGTGCGtt > 1:1223375/1‑69 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGTTTTGCCAGCGTTTGCGCGTTATCg < 1:1664241/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGTCAGCGTTTGCGCGTTATCg < 1:1225780/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:1181463/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:1054738/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:273908/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:3306582/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:496475/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:2665164/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:2525703/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:1669179/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:1408956/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:1610757/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:2083445/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:2077750/68‑1 (MQ=255)
ggTAATACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:1405045/68‑1 (MQ=255)
gTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGtt < 1:1620800/69‑1 (MQ=255)
gTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGtt < 1:2112080/69‑1 (MQ=255)
gTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGtt < 1:3363283/69‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:1538619/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:3252704/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:3149861/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:3310230/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:1576160/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:922207/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:2861450/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:48579/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:2282212/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:2667918/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:885463/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:520911/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:2357477/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACt > 1:899429/1‑37 (MQ=38)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:630055/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:937490/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:862962/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:3285503/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:968321/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:2410142/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:1035051/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:1135026/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:1286156/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:1439721/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:1716713/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:2169912/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:253241/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:2569465/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:3192648/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:2968190/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:2886750/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:2774197/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:281635/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTAGCCAGCGt < 1:1552424/52‑1 (MQ=255)
aaTACGGCATCACGCCCGCATCGAACAACGAATTACt > 1:1939076/1‑37 (MQ=25)
aTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:230221/51‑1 (MQ=255)
aTACGGCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:579266/51‑1 (MQ=255)
aCGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACAt > 1:3026657/1‑69 (MQ=255)
aCGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACAt > 1:963285/1‑69 (MQ=255)
cGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATc > 1:2011956/1‑69 (MQ=255)
cGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATc > 1:2729465/1‑69 (MQ=255)
cGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATc > 1:3220456/1‑69 (MQ=255)
ggCATCACGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGt < 1:918620/47‑1 (MQ=39)
caCGCCCTCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGCGCGTTATCg < 1:484139/54‑1 (MQ=255)
aCGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTca > 1:3399781/1‑58 (MQ=255)
aCGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGt > 1:1170055/1‑66 (MQ=255)
aCGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGt > 1:2706764/1‑66 (MQ=255)
|
CACTCACCATAAAATGCGCCGCGCCCTGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGT > minE/2836422‑2836549
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
