Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,849,439 |
A→G |
61.2% |
L532L (TTA→CTA) ‡ |
yjeP ← |
predicted mechanosensitive channel |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,849,439 | 0 | A | G | 61.2%
| 47.8
/ 57.9
| 90 | L532L (TTA→CTA) ‡ | yjeP | predicted mechanosensitive channel |
| Reads supporting (aligned to +/- strand): ref base A (12/23); new base G (28/27); total (40/50) |
| Fisher's exact test for biased strand distribution p-value = 1.34e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 |
CAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGA > minE/2849380‑2849494
|
cAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGAcgc < 1:3294596/69‑1 (MQ=255)
gCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCg > 1:3010554/1‑69 (MQ=255)
gCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCg > 1:394533/1‑69 (MQ=255)
cTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGaa < 1:2941652/69‑1 (MQ=255)
cTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGaa < 1:2878756/69‑1 (MQ=255)
cTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGaa < 1:2789904/69‑1 (MQ=255)
cTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGaa < 1:1209160/69‑1 (MQ=255)
cTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGaa < 1:526849/69‑1 (MQ=255)
cTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGaa < 1:2005999/69‑1 (MQ=255)
cTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGaa < 1:758859/69‑1 (MQ=255)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGaaaa < 1:1010508/69‑1 (MQ=255)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGaaaa < 1:2830446/69‑1 (MQ=255)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGaaaa < 1:2155012/69‑1 (MQ=255)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGaaaa < 1:835744/69‑1 (MQ=255)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGAcg > 1:2375218/1‑48 (MQ=37)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGcgac > 1:3251745/1‑52 (MQ=37)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGcgac > 1:2247250/1‑52 (MQ=37)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGaa > 1:191439/1‑57 (MQ=38)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:3206656/1‑67 (MQ=255)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:2623081/1‑67 (MQ=255)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:102835/1‑67 (MQ=255)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:3228621/1‑67 (MQ=255)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:675827/1‑67 (MQ=255)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:935083/1‑67 (MQ=255)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:3304974/1‑67 (MQ=255)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:3099172/1‑67 (MQ=255)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:900757/1‑67 (MQ=255)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:2927585/1‑67 (MQ=255)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:1021139/1‑67 (MQ=255)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:577664/1‑67 (MQ=255)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:1047415/1‑67 (MQ=255)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:696125/1‑67 (MQ=255)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:1815414/1‑67 (MQ=255)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:1631528/1‑67 (MQ=255)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:1607266/1‑67 (MQ=255)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:726438/1‑67 (MQ=255)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:193267/1‑67 (MQ=255)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:1956539/1‑67 (MQ=255)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:1974755/1‑67 (MQ=255)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:2069313/1‑67 (MQ=255)
cgcgCAAGCCGTAACCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGa > 1:3307516/1‑66 (MQ=255)
cgcgCAAGCCGTAAACCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGaa > 1:1924270/1‑67 (MQ=255)
ccGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTgcgc < 1:1950881/69‑1 (MQ=255)
ccGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTgcgc < 1:261578/69‑1 (MQ=255)
ccGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTgcgc < 1:2065956/69‑1 (MQ=255)
ccGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTgcgc < 1:1372000/69‑1 (MQ=255)
aCCCAGCGTCATCCACAGCACAGGCCGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:717044/69‑1 (MQ=255)
aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGAGGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:857916/69‑1 (MQ=255)
aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:963446/69‑1 (MQ=255)
aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:948170/69‑1 (MQ=255)
aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:310667/69‑1 (MQ=255)
aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:315634/69‑1 (MQ=255)
aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:3223272/69‑1 (MQ=255)
aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:893727/69‑1 (MQ=255)
aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:568812/69‑1 (MQ=255)
aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:8757/69‑1 (MQ=255)
aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:2941483/69‑1 (MQ=255)
aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:1885324/69‑1 (MQ=255)
aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:1945041/69‑1 (MQ=255)
aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:2173155/69‑1 (MQ=255)
aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:153473/69‑1 (MQ=255)
aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:2332320/69‑1 (MQ=255)
aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:1332738/69‑1 (MQ=255)
aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:1280785/69‑1 (MQ=255)
aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:2422952/69‑1 (MQ=255)
aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:2517510/69‑1 (MQ=255)
aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:2545329/69‑1 (MQ=255)
aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:2580048/69‑1 (MQ=255)
aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:1085541/69‑1 (MQ=255)
aCCCAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:2783877/69‑1 (MQ=255)
cccAGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:2534561/68‑1 (MQ=255)
aGCGTCATCCACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:283303/65‑1 (MQ=255)
ccACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTg < 1:2589377/69‑1 (MQ=255)
ccACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTg < 1:1105471/69‑1 (MQ=255)
ccACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTg < 1:3018518/69‑1 (MQ=255)
ccACAGCACAGGCAGCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGTAACGt < 1:1879777/57‑1 (MQ=255)
cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGGCAGAAGTGa < 1:2263383/69‑1 (MQ=255)
cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTg > 1:2635357/1‑68 (MQ=255)
cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa > 1:1644688/1‑69 (MQ=255)
cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa > 1:1855052/1‑69 (MQ=255)
cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa < 1:1011114/69‑1 (MQ=255)
cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa > 1:796199/1‑69 (MQ=255)
cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa > 1:1911952/1‑69 (MQ=255)
cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa > 1:642764/1‑69 (MQ=255)
cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa > 1:2253769/1‑69 (MQ=255)
cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa > 1:2362629/1‑69 (MQ=255)
cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa > 1:3392612/1‑69 (MQ=255)
cacaGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa > 1:318481/1‑69 (MQ=255)
caGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAag < 1:2930818/42‑1 (MQ=255)
aCCGGTAACGGTGACGCGACGAGAATCGACCAGaaaa < 1:2766777/37‑1 (MQ=255)
|
CAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGA > minE/2849380‑2849494
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
