Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,865,714 |
G→A |
37.9% |
A251A (GCG→GCA) |
hflC → |
modulator for HflB protease specific for phage lambda cII repressor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,865,714 | 0 | G | A | 37.9%
| 45.4
/ 49.0
| 79 | A251A (GCG→GCA) | hflC | modulator for HflB protease specific for phage lambda cII repressor |
| Reads supporting (aligned to +/- strand): ref base G (28/21); new base A (4/26); total (32/47) |
| Fisher's exact test for biased strand distribution p-value = 1.26e-04 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01 |
GCCGAGCGTGAAGCGGTAGCGCGTCGTCACCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTGAGCGTCAGGGCCGCATCATGCG > minE/2865646‑2865776
|
gcCGAGCGTGAAGCGGTAGCGCGTCGTCACCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGcgcgcg < 1:3177176/69‑1 (MQ=255)
gcCGAGCGTGAAGCGGTAGCGCGTCGTCACCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGcgcgcg < 1:603558/69‑1 (MQ=255)
cGTGAAGCGGTAGCGCGTCGTCACCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGTGCAACTGcc > 1:862094/1‑69 (MQ=255)
cGTGAAGCGGTAGCGCGTCGTCACCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGTGCAACTGcc > 1:2698775/1‑69 (MQ=255)
cGTGAAGCGGTAGCGCGTCGTCACCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGTGCAACTGcc > 1:1580532/1‑69 (MQ=255)
cGTGAAGCGGTAGCGCGTCGTCACCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGTGCAACTGcc > 1:1537524/1‑69 (MQ=255)
ggTAGCGCGTCGTCACCGTTCACAAGGTCAGGACGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGa < 1:3363708/69‑1 (MQ=255)
ggTAGCGCGTCGTCACCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGa < 1:1666100/69‑1 (MQ=255)
ggTAGCGCGTCGTCACCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGa < 1:2566287/69‑1 (MQ=255)
ggTAGCGCGTCGTCACCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGa < 1:2643381/69‑1 (MQ=255)
ggTAGCGCGTCGTCACCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGa < 1:2657188/69‑1 (MQ=255)
ggTAGCGCGTCGTCACCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGa < 1:2308629/69‑1 (MQ=255)
ggTAGCGCGTCGTCACCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGa < 1:2078322/69‑1 (MQ=255)
ggTAGCGCGTCGTCACCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGa < 1:1982601/69‑1 (MQ=255)
ggTAGCGCGTCGTCACCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGa < 1:3026871/69‑1 (MQ=255)
ggTAGCGCGTCGTCACCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGa < 1:870478/69‑1 (MQ=255)
ggTAGCGCGTCGTCACCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGa < 1:811138/69‑1 (MQ=255)
ggTAGCGCGTCGTCACCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGa < 1:78542/69‑1 (MQ=255)
ggTAGCGCGTCGTCACCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGa < 1:779052/69‑1 (MQ=255)
gcgcGTCGTCACCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTg < 1:556095/69‑1 (MQ=255)
gcgcGTCGTCACCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTg < 1:508996/69‑1 (MQ=255)
gcgcGTCGTCACCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTg < 1:1207248/69‑1 (MQ=255)
gcgcGTCGTCACCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTg < 1:2758024/69‑1 (MQ=255)
aCCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGCCCAGAActc > 1:1049346/1‑67 (MQ=255)
aCCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAACGc > 1:800513/1‑69 (MQ=255)
aCCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAACGc > 1:1556449/1‑69 (MQ=255)
aCCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAACGc > 1:1096832/1‑69 (MQ=255)
aCCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAACGc > 1:412718/1‑69 (MQ=255)
aCCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAACGc > 1:2739139/1‑69 (MQ=255)
aCCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAACGc > 1:2230606/1‑69 (MQ=255)
aCCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAACGc > 1:1517876/1‑69 (MQ=255)
aCCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAACGc > 1:276630/1‑69 (MQ=255)
aCCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAACGc > 1:2922826/1‑69 (MQ=255)
aCCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAACGc > 1:1823540/1‑69 (MQ=255)
aCCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAACGc > 1:1363403/1‑69 (MQ=255)
aCCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAAAGc > 1:1943490/1‑69 (MQ=255)
aCCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGAACAGAACGc > 1:695219/1‑69 (MQ=255)
aCCGTTCACAAGGTCAGCAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAACg > 1:3201538/1‑68 (MQ=255)
cacaAGGTCAGGAAGAAGCGGAAAAACTGCGTGCAACTGcc < 1:3277817/41‑1 (MQ=38)
cacaAGGTCAGGAAGAAGCGGAAAAACTGCGTGCAACTGcc < 1:939221/41‑1 (MQ=38)
cacaAGGTCAGGAAGAAGCGGAAAAACTGCGTGCAACTGcc < 1:262872/41‑1 (MQ=38)
cacaAGGTCAGGAAGAAGCGGAAAAACTGCGTGCAACTGcc < 1:2417047/41‑1 (MQ=38)
cacaAGGTCAGGAAGAAGCGGAAAAACTGCGTGCAACTGcc < 1:1121494/41‑1 (MQ=38)
cacaAGGTCAGGAAGAAGCGGAAAAACTGCGTGCAACTGcc < 1:1593263/41‑1 (MQ=38)
cacaAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAg < 1:2690545/58‑1 (MQ=255)
cacaAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAg < 1:1344114/58‑1 (MQ=255)
gTCAGGAAGAAGCGGAAAAACTGCGTGCAACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTg < 1:1051969/69‑1 (MQ=255)
gTCAGGAAGAAGCGGAAAAACTGCGTGCAACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTg < 1:910505/69‑1 (MQ=255)
gTCAGGAAGAAGCGGAAAAACTGCGTGCAACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTg < 1:1142190/69‑1 (MQ=255)
gTCAGGAAGAAGCGGAAAAACTGCGTGCAACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTg < 1:1278549/69‑1 (MQ=255)
gTCAGGAAGAAGCGGAAAAACTGCGTGCAACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTg < 1:65940/69‑1 (MQ=255)
gTCAGGAAGAAGCGGAAAAACTGCGTGCAACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTg < 1:603687/69‑1 (MQ=255)
gTCAGGAAGAAGCGGAAAAACTGCGTGCAACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTg < 1:1581911/69‑1 (MQ=255)
gTCAGGAAGAAGCGGAAAAACTGCGTGCAACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTg < 1:43306/69‑1 (MQ=255)
gTCAGGAAGAAGCGGAAAAACTGCGTGCAACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTg < 1:3374181/69‑1 (MQ=255)
gTCAGGAAGAAGCGGAAAAACTGCGTGCAACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTg < 1:3286699/69‑1 (MQ=255)
gTCAGGAAGAAGCGGAAAAACTGCGTGCAACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTg < 1:1766242/69‑1 (MQ=255)
gTCAGGAAGAAGCGGAAAAACTGCGTGCAACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTg < 1:1863827/69‑1 (MQ=255)
gTCAGGAAGAAGCGGAAAAACTGCGTGCAACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTg < 1:2651902/69‑1 (MQ=255)
gTCAGGAAGAAGCGGAAAAACTGCGTGCAACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTg < 1:238898/69‑1 (MQ=255)
gTCAGGAAGAAGCGGAAAAACTGCGTGCAACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTg < 1:2023285/69‑1 (MQ=255)
gTCAGGAAGAAGCGGAAAAACTGCGTGCAACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTg < 1:1942106/69‑1 (MQ=255)
gTCAGGAAGAAGCGGAAAAACTGCGTGCAACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTg < 1:199465/69‑1 (MQ=255)
gTCAGGAAGAAGCGGAAAAACTGCGTGCAACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTg < 1:3224500/69‑1 (MQ=255)
cAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTGAg > 1:2409814/1‑69 (MQ=255)
cAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTGAg > 1:2053463/1‑69 (MQ=255)
cAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTGAg > 1:1578873/1‑69 (MQ=255)
aGGAAGAAGCGGAAAAACTGCGTGCAACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTg < 1:2274666/66‑1 (MQ=255)
aagCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAg < 1:1582517/43‑1 (MQ=255)
gAAAAACTGCGTGCAACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTg < 1:2848238/55‑1 (MQ=255)
aaaaCTGCGCGCGACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTGAGCGTCAGGGCCGcat < 1:2489220/69‑1 (MQ=255)
gcgcgcGACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAg > 1:1891970/1‑44 (MQ=255)
gcgcgcGACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTGAGCGTCAGGGCCGCATCATGCg > 1:3036044/1‑69 (MQ=255)
gcgcgcGACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTGAGCGTCAGGGCCGCATCATGCg > 1:659558/1‑69 (MQ=255)
gcgcgcGACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTGAGCGTCAGGGCCGCATCATGCg > 1:1524815/1‑69 (MQ=255)
gcgcgcGACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTGAGCGTCAGGGCCGCATCATGCg > 1:344216/1‑69 (MQ=255)
gcgcgcGACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTGAGCGTCAGGGCCGCATCATGCg > 1:1333052/1‑69 (MQ=255)
gcgcgcGACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTGAGCGTCAGGGCCGCATCATGCg > 1:3120740/1‑69 (MQ=255)
gcgcgcGACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTGAGCGTCAGGGCCGCATCATGCg > 1:1731994/1‑69 (MQ=255)
gcgcgcGACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTGAGCGTCAGGGCCGCATCATGCg > 1:108763/1‑69 (MQ=255)
gcgcgcGACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTGAGCGTCAGGGCCGCATCATGCg > 1:3282256/1‑69 (MQ=255)
|
GCCGAGCGTGAAGCGGTAGCGCGTCGTCACCGTTCACAAGGTCAGGAAGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTGAGCGTCAGGGCCGCATCATGCG > minE/2865646‑2865776
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
