Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,879,384 |
T→C |
21.1% |
P214P (CCA→CCG) |
ytfE ← |
predicted regulator of cell morphogenesis and cell wall metabolism |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,879,384 | 0 | T | C | 21.1%
| 135.4
/ 27.7
| 99 | P214P (CCA→CCG) | ytfE | predicted regulator of cell morphogenesis and cell wall metabolism |
| Reads supporting (aligned to +/- strand): ref base T (32/46); new base C (12/9); total (44/55) |
| Fisher's exact test for biased strand distribution p-value = 2.21e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.40e-01 |
AACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCATT > minE/2879317‑2879443
|
aaCCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGt > 1:2339543/1‑68 (MQ=255)
aTCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTa > 1:475715/1‑69 (MQ=255)
aTCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTa > 1:3200889/1‑69 (MQ=255)
aTCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTa > 1:2911271/1‑69 (MQ=255)
aTCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTa > 1:36363/1‑69 (MQ=255)
aTCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTa > 1:3265808/1‑69 (MQ=255)
aTCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTa > 1:915685/1‑69 (MQ=255)
aTCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTa > 1:1551885/1‑69 (MQ=255)
aTCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTa > 1:3304868/1‑69 (MQ=255)
aTCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTa > 1:841510/1‑69 (MQ=255)
cTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCTCGCGCGGGAATAGTAc < 1:3131044/64‑1 (MQ=255)
cTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGt > 1:217122/1‑69 (MQ=255)
cTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGt > 1:2236991/1‑69 (MQ=255)
cTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGt > 1:2253824/1‑69 (MQ=255)
cTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTAc < 1:1532088/64‑1 (MQ=255)
cTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTAc < 1:2103278/64‑1 (MQ=255)
cTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTAc < 1:2366529/64‑1 (MQ=255)
cTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTAc < 1:2639575/64‑1 (MQ=255)
cTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTAc < 1:1281040/64‑1 (MQ=255)
cTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTAc < 1:2789242/64‑1 (MQ=255)
ggATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGttt > 1:860433/1‑69 (MQ=255)
ggATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGttt > 1:978444/1‑69 (MQ=255)
ggATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGttt > 1:2337038/1‑69 (MQ=255)
ggATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGttt > 1:2486267/1‑69 (MQ=255)
ggATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGttt > 1:2820051/1‑69 (MQ=255)
ggATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGttt > 1:1205638/1‑69 (MQ=255)
ggATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGttt > 1:867696/1‑69 (MQ=255)
ggATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGttt > 1:552790/1‑69 (MQ=255)
ggCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGTGAACAGTACATTGTTTTCCa < 1:1751567/69‑1 (MQ=255)
ggCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCa < 1:2781767/69‑1 (MQ=255)
ggCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCa < 1:2439673/69‑1 (MQ=255)
ggCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCa < 1:3212421/69‑1 (MQ=255)
ggCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCa < 1:159193/69‑1 (MQ=255)
ggCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCa < 1:1979121/69‑1 (MQ=255)
ggCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCa < 1:1368576/69‑1 (MQ=255)
cgcAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTAc < 1:839624/56‑1 (MQ=255)
gcAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGa > 1:1993383/1‑68 (MQ=255)
gcAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGa > 1:1220018/1‑68 (MQ=255)
gcAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGa > 1:669422/1‑68 (MQ=255)
cAATGCCATCCAGCTTTTAGATAACTCACCCGCCAGCGCGCGCGGGAATAGTAc < 1:876220/54‑1 (MQ=255)
aTGCCATCCAGCTTTTAGATCACTCACCCGCCCGCGCGCGCGGGAATAGTACATTGt > 1:167874/1‑57 (MQ=255)
aTGCCATCCAGCTTTTAGATCACTCACCCGCCCGCGCGCGCGGGAATAGTACATTGt > 1:94400/1‑57 (MQ=255)
aTGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTACATTGt > 1:1548665/1‑57 (MQ=255)
aTGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTACATTGt > 1:1163450/1‑57 (MQ=255)
aTGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTACATTGt > 1:2712921/1‑57 (MQ=255)
aTGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTACATTGt > 1:1311631/1‑57 (MQ=255)
aTGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTACATTGt > 1:3033353/1‑57 (MQ=255)
aTGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTACATTGt > 1:656970/1‑57 (MQ=255)
aTGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTACATTGt > 1:3076915/1‑57 (MQ=255)
aTGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTACATTGt > 1:2162992/1‑57 (MQ=255)
aTGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTACATTGt > 1:737114/1‑57 (MQ=255)
aTGCCATCCAGCTTTTAGATCACTCACCCGCAAGCGCGCGCGGGAATAGTACATTGt > 1:1444208/1‑57 (MQ=255)
ccATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGAtgt > 1:762438/1‑69 (MQ=255)
ccATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGAtgt > 1:3290534/1‑69 (MQ=255)
ccATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGAtgt > 1:320997/1‑69 (MQ=255)
ccATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGAtgt > 1:1059265/1‑69 (MQ=255)
ccATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGAtgt > 1:2731143/1‑69 (MQ=255)
ccATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGAtgt > 1:2212209/1‑69 (MQ=255)
ccATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGAtgt > 1:1056003/1‑69 (MQ=255)
ccATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGAtgt > 1:1636119/1‑69 (MQ=255)
aTCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGAtgtg < 1:1089570/68‑1 (MQ=255)
aTCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGAtgtg < 1:1527074/68‑1 (MQ=255)
aTCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGAtgtg < 1:1672241/68‑1 (MQ=255)
aTCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGAtgtg < 1:1669579/68‑1 (MQ=255)
cAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACt > 1:2708597/1‑59 (MQ=255)
cAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATc < 1:1525886/68‑1 (MQ=255)
aTCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGtca < 1:880771/68‑1 (MQ=255)
tcactcacCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGtcatc < 1:701253/69‑1 (MQ=255)
tcactcacCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGtcatc < 1:641653/69‑1 (MQ=255)
tcactcacCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGtcatc < 1:498819/69‑1 (MQ=255)
tcactcacCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGtcatc < 1:1992695/69‑1 (MQ=255)
tcactcacCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGtcatc < 1:2130975/69‑1 (MQ=255)
tcactcacCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGtcatc < 1:2639699/69‑1 (MQ=255)
tcactcacCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGtcatc < 1:2795345/69‑1 (MQ=255)
cactcacCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGtc < 1:3238180/65‑1 (MQ=255)
cAGCGCGCGTTGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:1383145/69‑1 (MQ=255)
cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTGAtt < 1:1899181/69‑1 (MQ=255)
cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:785079/69‑1 (MQ=255)
cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:841170/69‑1 (MQ=255)
cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:985433/69‑1 (MQ=255)
cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:2597424/69‑1 (MQ=255)
cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:1358230/69‑1 (MQ=255)
cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:1485373/69‑1 (MQ=255)
cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:1593173/69‑1 (MQ=255)
cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:1637289/69‑1 (MQ=255)
cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:1727464/69‑1 (MQ=255)
cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:1917851/69‑1 (MQ=255)
cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:197724/69‑1 (MQ=255)
cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:2352352/69‑1 (MQ=255)
cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:235529/69‑1 (MQ=255)
cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:588266/69‑1 (MQ=255)
cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:265121/69‑1 (MQ=255)
cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:2773708/69‑1 (MQ=255)
cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:294465/69‑1 (MQ=255)
cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:3129928/69‑1 (MQ=255)
cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:3213891/69‑1 (MQ=255)
cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:3331190/69‑1 (MQ=255)
cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:36056/69‑1 (MQ=255)
cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:583369/69‑1 (MQ=255)
aGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:628070/68‑1 (MQ=255)
|
AACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCATT > minE/2879317‑2879443
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
