Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,903,583 |
A→G |
35.5% |
H154H (CAT→CAC) |
nrdD ← |
anaerobic ribonucleoside‑triphosphate reductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,903,583 | 0 | A | G | 35.5%
| 57.1
/ 54.0
| 90 | H154H (CAT→CAC) | nrdD | anaerobic ribonucleoside‑triphosphate reductase |
| Reads supporting (aligned to +/- strand): ref base A (40/18); new base G (14/18); total (54/36) |
| Fisher's exact test for biased strand distribution p-value = 2.53e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATCCC > minE/2903517‑2903645
|
cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCAtg > 1:965125/1‑69 (MQ=255)
cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCAtg > 1:300985/1‑69 (MQ=255)
cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCAtg > 1:2094101/1‑69 (MQ=255)
cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCAtg > 1:397836/1‑69 (MQ=255)
cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCAtg > 1:813188/1‑69 (MQ=255)
cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCAtg > 1:1419815/1‑69 (MQ=255)
cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCAtg > 1:2270793/1‑69 (MQ=255)
cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCAtg > 1:1324949/1‑69 (MQ=255)
cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCAtg > 1:2439716/1‑69 (MQ=255)
cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCAtg > 1:1161326/1‑69 (MQ=255)
cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCAtg > 1:3354487/1‑69 (MQ=255)
cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCAtg > 1:3375480/1‑69 (MQ=255)
cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCAtg > 1:1097449/1‑69 (MQ=255)
cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCAtg > 1:2707275/1‑69 (MQ=255)
gAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGcac < 1:2942005/69‑1 (MQ=255)
aaCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgc > 1:2713183/1‑69 (MQ=255)
aaCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgc > 1:2732617/1‑69 (MQ=255)
aaCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgc > 1:1005605/1‑69 (MQ=255)
aaCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgc > 1:2827846/1‑69 (MQ=255)
aaCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgc > 1:2046734/1‑69 (MQ=255)
aaCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgc > 1:266642/1‑69 (MQ=255)
aaCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgc > 1:2976885/1‑69 (MQ=255)
aaCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgc > 1:490738/1‑69 (MQ=255)
aaCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgc > 1:3028013/1‑69 (MQ=255)
aaCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgc > 1:3346024/1‑69 (MQ=255)
aaCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgc > 1:93032/1‑69 (MQ=255)
aaCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgc > 1:1260329/1‑69 (MQ=255)
aaCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgc > 1:1177382/1‑69 (MQ=255)
aaCGGTGAGTAATCGAGATCCTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgc > 1:1049497/1‑69 (MQ=255)
aCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTg < 1:1740041/52‑1 (MQ=255)
aCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTg > 1:1482052/1‑52 (MQ=255)
aCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTg > 1:2797421/1‑52 (MQ=255)
aCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTg < 1:412635/52‑1 (MQ=255)
aCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTg < 1:2267731/52‑1 (MQ=255)
ggTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgcagc < 1:3340204/69‑1 (MQ=255)
tAATCGAGATCGTGATAGTGAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGGCAGCAGGTGc > 1:2842345/1‑69 (MQ=255)
tAATCGAGATCGTGATAGTGAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGGCAGCAGGTGc > 1:3058870/1‑69 (MQ=255)
tAATCGAGATCGTGATAGTGAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGGCAGCAGGTGc > 1:3424283/1‑69 (MQ=255)
tAATCGAGATCGTGATAGTGAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGGCAGCAGGTGc > 1:727157/1‑69 (MQ=255)
tAATCGAGATCGTGATAGTGAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGGCAGCAGGTGc > 1:1415074/1‑69 (MQ=255)
aaTCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGcac < 1:1453693/46‑1 (MQ=255)
cGAGATCGTGATAGTGAATATCGCCACGCTCATGTTCCTGCACCACGTCACGCGGCAGCAGGTGCTGa < 1:752797/68‑1 (MQ=255)
cGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGa < 1:3322060/68‑1 (MQ=255)
cGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGa < 1:1717780/68‑1 (MQ=255)
cGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGa < 1:2504187/68‑1 (MQ=255)
cGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGAc > 1:2795689/1‑69 (MQ=255)
cGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCAt < 1:608185/69‑1 (MQ=255)
aGTGAATATCGCCCCGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAgtg > 1:1134054/1‑67 (MQ=255)
aGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAgtg > 1:2072270/1‑67 (MQ=255)
aGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAgtg > 1:1130549/1‑67 (MQ=255)
aGTGAATATCGCCACGCTCATCTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGCCGTGCATAgt > 1:147674/1‑66 (MQ=255)
gTGAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGGCAGCAGGTGCt > 1:2863283/1‑53 (MQ=255)
gTGAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGGCGTGCATAGTGttt > 1:3107704/1‑69 (MQ=255)
gTGAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGGCGTGCATAGTGttt > 1:767844/1‑69 (MQ=255)
gTGAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGGCGTGCATAGTGttt > 1:2043813/1‑69 (MQ=255)
gTGAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGGCGTGCATAGTGttt > 1:74047/1‑69 (MQ=255)
gTGAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGGCGTGCATAGTGttt > 1:1603377/1‑69 (MQ=255)
gTGAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGGCGTGCATAGTGttt > 1:2057744/1‑69 (MQ=255)
gTGAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGGCGTGCATAGTGttt > 1:2313796/1‑69 (MQ=255)
gTGAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGGCGTGCATAGTGttt > 1:259270/1‑69 (MQ=255)
gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:27521/44‑1 (MQ=38)
gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:781653/44‑1 (MQ=38)
gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:367212/44‑1 (MQ=38)
gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:604843/44‑1 (MQ=38)
gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:681511/44‑1 (MQ=38)
gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:632386/44‑1 (MQ=38)
gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:619488/44‑1 (MQ=38)
gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:610409/44‑1 (MQ=38)
gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:328785/44‑1 (MQ=38)
gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:3283927/44‑1 (MQ=38)
gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:3118191/44‑1 (MQ=38)
gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:1351355/44‑1 (MQ=38)
gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:2724906/44‑1 (MQ=38)
gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:2478761/44‑1 (MQ=38)
gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:1449622/44‑1 (MQ=38)
gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:3392844/44‑1 (MQ=38)
gAATATCACCACGTTCGGGTGCCTGCACCACGTCACGCGgcagc < 1:1534225/44‑1 (MQ=25)
aaTATCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGc > 1:1531806/1‑69 (MQ=255)
aaTATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:500122/43‑1 (MQ=37)
atCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCAc < 1:1423845/69‑1 (MQ=255)
tCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACg < 1:2119169/69‑1 (MQ=255)
tCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACg < 1:857744/69‑1 (MQ=255)
tCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACg < 1:1491156/69‑1 (MQ=255)
tCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACg < 1:1561320/69‑1 (MQ=255)
tCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACg < 1:2432990/69‑1 (MQ=255)
tCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACg < 1:2642155/69‑1 (MQ=255)
aCGCTCATGTGCCTGCACCACGTCACGCTGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATccc > 1:694119/1‑69 (MQ=255)
aCGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGCTccc > 1:1011406/1‑69 (MQ=255)
aCGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATccc > 1:2831403/1‑69 (MQ=255)
aCGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATccc > 1:2994861/1‑69 (MQ=255)
|
CATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATCCC > minE/2903517‑2903645
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
