Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,910,358 |
C→T |
44.3% |
R450R (CGC→CGT) |
mgtA → |
magnesium transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,910,358 | 0 | C | T | 44.3%
| 23.2
/ 68.0
| 88 | R450R (CGC→CGT) | mgtA | magnesium transporter |
| Reads supporting (aligned to +/- strand): ref base C (29/20); new base T (15/24); total (44/44) |
| Fisher's exact test for biased strand distribution p-value = 8.54e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGC > minE/2910295‑2910422
|
aGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGAt > 1:949883/1‑69 (MQ=255)
aGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGAt > 1:940956/1‑69 (MQ=255)
aGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGAt > 1:1823875/1‑69 (MQ=255)
aGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGAt > 1:2806331/1‑69 (MQ=255)
aGCGCGCTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGAt < 1:2604718/69‑1 (MQ=255)
aGCGCGCTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGAt < 1:355498/69‑1 (MQ=255)
aGCGCGCTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGAt < 1:1637166/69‑1 (MQ=255)
aGCGCGCTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGAt < 1:3053384/69‑1 (MQ=255)
aGCGCGCTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGAt < 1:1366962/69‑1 (MQ=255)
aGCGCGCTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGAt < 1:2713539/69‑1 (MQ=255)
gcgcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGAt < 1:32111/68‑1 (MQ=255)
cgcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTT‑ATTTCGAGCGTCGTCGGATGTc < 1:1199823/69‑1 (MQ=255)
gcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTc < 1:283418/69‑1 (MQ=255)
gcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTc < 1:2730255/69‑1 (MQ=255)
gcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTc < 1:2595114/69‑1 (MQ=255)
gcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTc < 1:3035407/69‑1 (MQ=255)
gcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTc < 1:2688809/69‑1 (MQ=255)
gcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTc < 1:2262728/69‑1 (MQ=255)
gcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTc < 1:1107283/69‑1 (MQ=255)
gcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTc < 1:2181902/69‑1 (MQ=255)
gcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTc < 1:3282355/69‑1 (MQ=255)
gcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTc < 1:2113485/69‑1 (MQ=255)
gcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTc < 1:935915/69‑1 (MQ=255)
gcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTc < 1:1667235/69‑1 (MQ=255)
gcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTc < 1:636429/69‑1 (MQ=255)
gcgcTCGCTGGCCAGCCGTTGGCAAAAAATTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTc < 1:1377263/69‑1 (MQ=255)
cGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGt > 1:3066839/1‑62 (MQ=255)
cGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTg > 1:410001/1‑63 (MQ=255)
cGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTg > 1:550551/1‑63 (MQ=255)
cGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTg > 1:1291749/1‑63 (MQ=255)
cGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTg > 1:2880474/1‑63 (MQ=255)
cGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTg > 1:2910121/1‑63 (MQ=255)
cGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTg > 1:1489081/1‑63 (MQ=255)
tGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAAt < 1:2504214/69‑1 (MQ=255)
tGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAAt < 1:904131/69‑1 (MQ=255)
tGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAAt < 1:889771/69‑1 (MQ=255)
tGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAAt < 1:2561078/69‑1 (MQ=255)
tGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAAt < 1:3328332/69‑1 (MQ=255)
tGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAAt < 1:2385753/69‑1 (MQ=255)
tGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAAt < 1:2205570/69‑1 (MQ=255)
aaaaaaTTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCg < 1:824636/69‑1 (MQ=255)
aaaaaaTTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCg < 1:1639146/69‑1 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTCggtggt > 1:2937491/1‑52 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTCggtggt > 1:2962636/1‑52 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTCggtggt > 1:2991938/1‑52 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTCggtggt > 1:2720282/1‑52 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTCggtggt > 1:1128311/1‑52 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTCggtggt > 1:2694299/1‑52 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTCggtggt > 1:263906/1‑52 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTCggtggt > 1:1465577/1‑52 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTCggtggt > 1:2143238/1‑52 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTCggtggt > 1:1155714/1‑52 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTCggtggt > 1:2381738/1‑52 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTCggtggt > 1:2380716/1‑52 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTCggtgg > 1:3139104/1‑51 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGATGTCgg > 1:1610016/1‑48 (MQ=255)
aaaaaaTTGATGAGATTCCATTTGATTT‑‑AGCGTCGTCGGATGTCggtggt > 1:2666274/1‑50 (MQ=255)
aTTGATGAGATTCCATTTGATTTCGAGCGTCGTCGGAt < 1:3171727/38‑1 (MQ=38)
tgatgaGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCAcc < 1:2261261/68‑1 (MQ=255)
gatgaGATTCCATTTGATTTCGAGCGTCGTCGGAt < 1:2741647/35‑1 (MQ=255)
ttCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAgg < 1:793049/68‑1 (MQ=255)
ttCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAgg < 1:761401/68‑1 (MQ=255)
ttCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAgg < 1:2627048/68‑1 (MQ=255)
ttCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAgg < 1:3071541/68‑1 (MQ=255)
cGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATc > 1:244788/1‑46 (MQ=255)
cGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTTGTTTGCAAAGGTg > 1:942780/1‑64 (MQ=255)
cGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTg > 1:3205066/1‑64 (MQ=255)
cGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTg < 1:2258652/64‑1 (MQ=255)
cGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTg > 1:127577/1‑64 (MQ=255)
cGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTg < 1:2633952/64‑1 (MQ=255)
cGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTg > 1:2885670/1‑64 (MQ=255)
cGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTg < 1:2337479/64‑1 (MQ=255)
gTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAg > 1:3331224/1‑38 (MQ=255)
gTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGc > 1:3312041/1‑69 (MQ=255)
gTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGc > 1:933357/1‑69 (MQ=255)
gTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGc > 1:1278135/1‑69 (MQ=255)
gTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGc > 1:798593/1‑69 (MQ=255)
gTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGc > 1:944393/1‑69 (MQ=255)
gTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGc > 1:1632006/1‑69 (MQ=255)
gTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGc > 1:187354/1‑69 (MQ=255)
gTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGc > 1:1999997/1‑69 (MQ=255)
gTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGc < 1:3317776/69‑1 (MQ=255)
gTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGc > 1:3316103/1‑69 (MQ=255)
gTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGc < 1:2156684/69‑1 (MQ=255)
gTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGc > 1:3280236/1‑69 (MQ=255)
gTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGc > 1:2792115/1‑69 (MQ=255)
gTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGc < 1:2780422/69‑1 (MQ=255)
gTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCAACATCAGCTGGTTTGCAAAGGTGCATTGc > 1:1277393/1‑69 (MQ=255)
|
AGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGC > minE/2910295‑2910422
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
