Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,973,916 |
T→C |
31.1% |
R104R (CGT→CGC) |
nadR → |
bifunctional DNA‑binding transcriptional repressor and NMN adenylyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,973,916 | 0 | T | C | 31.1%
| 79.8
/ 46.2
| 93 | R104R (CGT→CGC) | nadR | bifunctional DNA‑binding transcriptional repressor and NMN adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (20/44); new base C (14/15); total (34/59) |
| Fisher's exact test for biased strand distribution p-value = 1.63e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 |
TACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGT > minE/2973848‑2973983
|
tACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGt > 1:1792875/1‑69 (MQ=255)
tACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGt > 1:2141262/1‑69 (MQ=255)
tACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGt > 1:1315836/1‑69 (MQ=255)
cAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGCGACcgcgcg < 1:3232797/69‑1 (MQ=255)
cAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGCGACcgcgcg < 1:1975762/69‑1 (MQ=255)
cAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGCGACcgcgcg < 1:2244697/69‑1 (MQ=255)
cAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGCGACcgcgcg < 1:2499952/69‑1 (MQ=255)
cAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGCGACcgcgcg < 1:2733873/69‑1 (MQ=255)
cAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGCGACcgcgcg < 1:1602092/69‑1 (MQ=255)
cAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGCGACcgcgcg < 1:1576596/69‑1 (MQ=255)
cAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGCGACcgcgcg < 1:400885/69‑1 (MQ=255)
cAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGCGACcgcgcg < 1:472235/69‑1 (MQ=255)
cAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGCGACcgcgcg < 1:756211/69‑1 (MQ=255)
cAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGCGACcgcgcg < 1:819974/69‑1 (MQ=255)
cAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGCGACcgcgcg < 1:1215358/69‑1 (MQ=255)
cAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGCGACcgcgcg < 1:89748/69‑1 (MQ=255)
cAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGCGACcgcgcg < 1:965855/69‑1 (MQ=255)
aGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGCGACcgcgcg < 1:1207122/68‑1 (MQ=255)
tGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGaa < 1:2954913/69‑1 (MQ=255)
gTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGCGACCGCGCgttgtttg > 1:1771240/1‑64 (MQ=255)
gTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGCGACCGCGCgttgtttg > 1:669204/1‑64 (MQ=255)
gTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGCGACCGCGCgttgtttg > 1:534143/1‑64 (MQ=255)
gTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGCGACCGCGCgttgtttg > 1:2526043/1‑64 (MQ=255)
gTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGCGACCGCGCgttgtttg > 1:3387385/1‑64 (MQ=255)
gTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGCGACCGCGCgttgtttg > 1:1705739/1‑64 (MQ=255)
gTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGCGACCGCGCgttgtttg > 1:2791842/1‑64 (MQ=255)
gTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGCGACCGCGCgttgtttg > 1:2177256/1‑64 (MQ=255)
gTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGCGACCGCGCgttgtttg > 1:2156196/1‑64 (MQ=255)
gTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGCGACCGCGCgttgtttg > 1:2867340/1‑64 (MQ=255)
tccAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGa < 1:3189779/66‑1 (MQ=255)
gCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGa < 1:2470990/67‑1 (MQ=255)
gCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGa < 1:247839/67‑1 (MQ=255)
gCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGa < 1:1697892/67‑1 (MQ=255)
gCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGa < 1:2680510/67‑1 (MQ=255)
gCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGa < 1:1707346/67‑1 (MQ=255)
gCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGa < 1:213863/67‑1 (MQ=255)
gCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGa < 1:2098440/67‑1 (MQ=255)
gCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGa < 1:2097324/67‑1 (MQ=255)
gCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGa < 1:3175578/67‑1 (MQ=255)
gCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGa < 1:325934/67‑1 (MQ=255)
gCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGa < 1:1458312/67‑1 (MQ=255)
gCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGa < 1:1278294/67‑1 (MQ=255)
gCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGAc < 1:2595549/68‑1 (MQ=255)
gCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGAc < 1:778125/68‑1 (MQ=255)
ccAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGa < 1:2580237/66‑1 (MQ=255)
cAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGAc < 1:2372746/66‑1 (MQ=255)
ggTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGc < 1:623469/69‑1 (MQ=255)
tGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCAt > 1:241201/1‑69 (MQ=255)
tGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCAt > 1:3126611/1‑69 (MQ=255)
tGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCAt < 1:2030476/69‑1 (MQ=255)
tGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCAt > 1:2013207/1‑69 (MQ=255)
tGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCAt > 1:378751/1‑69 (MQ=255)
tGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCAt < 1:2180976/69‑1 (MQ=255)
tGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCAt > 1:924539/1‑69 (MQ=255)
tGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCAt > 1:1463674/1‑69 (MQ=255)
tGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCAt < 1:2199429/69‑1 (MQ=255)
tGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCAt < 1:884341/69‑1 (MQ=255)
tGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCAt > 1:2374246/1‑69 (MQ=255)
gACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATg > 1:264620/1‑69 (MQ=255)
cTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCgc > 1:1939453/1‑67 (MQ=255)
cTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCgc > 1:562842/1‑67 (MQ=255)
cTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCgc > 1:2564059/1‑67 (MQ=255)
cTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCgc > 1:1435918/1‑67 (MQ=255)
cTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCgc > 1:960191/1‑67 (MQ=255)
gCATATCATTATGGGTCTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCgcagca > 1:2393215/1‑69 (MQ=255)
tatCATTATGGGTTTTGACTATACCCGTGACCGCGCGTTGTTCGAAGACAGTGc < 1:3146761/54‑1 (MQ=255)
tatCATTATGGGTTTTGACGATACGCGTGACCGCGCGTTGTTCGAAGACAGTGc < 1:1207906/54‑1 (MQ=255)
tatCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGc < 1:2654159/54‑1 (MQ=255)
tatCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGc < 1:677750/54‑1 (MQ=255)
tatCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGc < 1:1866826/54‑1 (MQ=255)
tatCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGc < 1:1880901/54‑1 (MQ=255)
tatCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGc < 1:2217895/54‑1 (MQ=255)
tatCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGc < 1:562215/54‑1 (MQ=255)
tatCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGc < 1:2608807/54‑1 (MQ=255)
tatCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGc < 1:3105538/54‑1 (MQ=255)
tatCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGc < 1:171514/54‑1 (MQ=255)
tatCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGc < 1:1615319/54‑1 (MQ=255)
tatCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGc < 1:2813345/54‑1 (MQ=255)
tatCATTATCGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGc < 1:2294639/54‑1 (MQ=255)
cATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGa > 1:2073900/1‑68 (MQ=255)
cATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGAc > 1:1487673/1‑69 (MQ=255)
gATACCCGCGACCGCGCGTTGTTTGAAGACAGCGCCATGTCGCAGCAGCCCACGGTGCCGGATCGTCt > 1:191601/1‑68 (MQ=255)
gATACCCGCGACCGCGCGTTGTTTGAAGACAGCGCCATGTCGCAGCAGCCCACGGTGCCGGATCGTCt > 1:2026209/1‑68 (MQ=255)
gATACCCGCGACCGCGCGTTGTTTGAAGACAGCGCCATGTCGCAGCAGCCCACGGTGCCGGATCGTCTg > 1:497485/1‑69 (MQ=255)
gATACCCGCGACCGCGCGTTGTTTGAAGACAGCGCCATGTCGCAGCAGCCCACGGTGCCGGATCGTCTg > 1:2153758/1‑69 (MQ=255)
cGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTg > 1:981808/1‑68 (MQ=255)
cGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTg > 1:1014677/1‑68 (MQ=255)
cGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTg > 1:620182/1‑68 (MQ=255)
cGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTg > 1:493184/1‑68 (MQ=255)
gTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGt < 1:653608/69‑1 (MQ=255)
gTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGt < 1:475585/69‑1 (MQ=255)
gTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGt < 1:2919372/69‑1 (MQ=255)
gTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGt < 1:2772378/69‑1 (MQ=255)
gTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGt < 1:2519104/69‑1 (MQ=255)
gTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGt < 1:2143417/69‑1 (MQ=255)
gTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGt < 1:1859020/69‑1 (MQ=255)
|
TACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGT > minE/2973848‑2973983
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
