Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F112 I0 R1 51 112.5 5500062 95.1% 5230558 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,400,313 A→C 100% D9A (GAT→GCT)  hfq → global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,400,3130AC92.0% 410.7 / 18.8 125D9A (GAT→GCT) hfqglobal sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication
Reads supporting (aligned to +/- strand):  ref base A (6/4);  new base C (63/52);  total (69/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.10e-01

Only 100 of 125 total aligned reads displayed.

AGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGA  >  NC_000913/4400216‑4400406
                                                                                                 |                                                                                             
agaCGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTc                                                                                             <  1:2319975/100‑1 (MQ=255)
  aCGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCg                                                                                           <  1:238719/100‑1 (MQ=255)
   cGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACGAGATCCGt                                                                                          >  1:90425/1‑100 (MQ=255)
   cGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGt                                                                                          >  1:2161822/1‑100 (MQ=255)
      aTCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTcc                                                                                       <  2:802284/100‑1 (MQ=255)
      aTCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTcc                                                                                       <  1:2145759/100‑1 (MQ=255)
        cGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTcccg                                                                                     >  1:90150/1‑98 (MQ=255)
        cGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTg                                                                                     >  2:377596/1‑100 (MQ=255)
         gTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTg                                                                                     >  2:543965/1‑99 (MQ=255)
          tGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGaa                                                                                   <  2:233222/100‑1 (MQ=255)
           gcgcAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAAc                                                                                  <  2:1324155/100‑1 (MQ=255)
             gcAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGc                                                                                >  2:2544960/1‑100 (MQ=255)
              cAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCa                                                                               >  1:2014161/1‑100 (MQ=255)
                aTTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACt                                                                             >  1:1389515/1‑100 (MQ=255)
                  tgtttCATAATCGAAAGGAAAAAAGGACAGTTGAGCATAGAAGGAAAAGAGAGAATGGCTAAGGGGCGACCTTTACATGCTCCGTTCTTCAACCCACtc                                                                            <  2:2668687/97‑2 (MQ=255)
                   ttttCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCg                                                                          >  2:900538/1‑100 (MQ=255)
                       cAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCgg                                                                      <  1:1015416/100‑1 (MQ=255)
                       cAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCgg                                                                      >  1:1195709/1‑100 (MQ=255)
                       cAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCgg                                                                      <  1:1342561/100‑1 (MQ=255)
                       cAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCgg                                                                      <  1:839641/100‑1 (MQ=255)
                       cAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCgg                                                                      >  1:687959/1‑100 (MQ=255)
                        aGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCggg                                                                     >  1:1778528/1‑100 (MQ=255)
                        aGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCggg                                                                     <  1:235871/100‑1 (MQ=255)
                         gAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCgg                                                                      >  2:2491303/1‑98 (MQ=255)
                         gAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGa                                                                    >  1:388232/1‑100 (MQ=255)
                         gAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGa                                                                    >  1:218427/1‑100 (MQ=255)
                           aTCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCCG‑ACGCAGTGCGttcaggaac                                                                 <  1:2728197/100‑9 (MQ=37)
                            tCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACg                                                                 <  1:1383703/100‑1 (MQ=255)
                            tCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACg                                                                 <  1:2165682/100‑1 (MQ=255)
                            tCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACg                                                                 >  2:1668893/1‑100 (MQ=255)
                            tCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCCG‑ACGCAGTGCGttcaggaacg                                                                <  2:2445667/100‑10 (MQ=37)
                              gAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACgtg                                                               >  1:1240718/1‑100 (MQ=255)
                              gAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACgtg                                                               >  2:2444088/1‑100 (MQ=255)
                                 aGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAATGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTc                                                            >  2:286124/1‑100 (MQ=255)
                                     tCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGt                                                        <  1:1286673/100‑1 (MQ=255)
                                     tCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGt                                                        <  1:1523526/100‑1 (MQ=255)
                                       aaaGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGttt                                                      >  1:1896751/1‑100 (MQ=255)
                                          gTACAAATACGCATATAAGGCAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCta                                                   >  2:1637751/1‑100 (MQ=255)
                                          gTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCta                                                   <  1:2268648/100‑1 (MQ=255)
                                            aCAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCtatt                                                 <  1:261220/100‑1 (MQ=255)
                                             cAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCtattt                                                >  1:1384516/1‑100 (MQ=255)
                                             cAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCtat                                                  >  2:1420614/1‑98 (MQ=255)
                                               aaTAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCtatttat                                              <  2:2645155/100‑1 (MQ=255)
                                               aaTAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCtatttat                                              >  1:1626039/1‑100 (MQ=255)
                                                 tAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTatttattt                                            >  2:1692592/1‑100 (MQ=255)
                                                  aaGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAATCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTg                                           <  1:542308/100‑1 (MQ=255)
                                                     cATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTg                                        <  2:623757/100‑1 (MQ=255)
                                                     cATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCGCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTg                                        <  2:672046/100‑1 (MQ=255)
                                                      atgtaAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTg                                        <  2:2270823/96‑1 (MQ=255)
                                                      atatAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGa                                       >  2:39121/1‑100 (MQ=255)
                                                       tataAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGaa                                      >  1:887804/1‑100 (MQ=255)
                                                       tataAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGaa                                      >  1:707105/1‑100 (MQ=255)
                                                        ataAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAAt                                     >  1:1616385/1‑100 (MQ=255)
                                                         taAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATg                                    >  1:2462471/1‑100 (MQ=255)
                                                         taAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATg                                    >  1:1821742/1‑100 (MQ=255)
                                                         taAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATg                                    <  2:1290237/100‑1 (MQ=255)
                                                         taAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTCTCTATTTATTTGGTGAATg                                    >  2:1896471/1‑100 (MQ=255)
                                                            ggAAAAGAGAGAATGGCTAAGGGGCAATCTTTACCAGCTCCGTTCCTGAACGCACTGCGTCGAGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTa                                 <  2:2330314/100‑1 (MQ=255)
                                                            ggAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTa                                 >  2:1522087/1‑100 (MQ=255)
                                                            ggAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTa                                 <  1:2262315/100‑1 (MQ=255)
                                                            ggAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTa                                 >  2:1993870/1‑100 (MQ=255)
                                                             gAAAAGAGAGAATGTCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACGGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTAt                                <  2:764133/100‑1 (MQ=255)
                                                             gAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTAt                                <  1:49601/100‑1 (MQ=255)
                                                             gAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTAt                                <  2:1896742/100‑1 (MQ=255)
                                                             gAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTAt                                <  2:2161808/100‑1 (MQ=255)
                                                               aaaGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGTAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTa                              >  2:1167138/1‑100 (MQ=255)
                                                               aaaGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTa                              >  1:2749277/1‑100 (MQ=255)
                                                                 agagagAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAg                            <  2:2694482/100‑1 (MQ=255)
                                                                  ttccgatctgCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGc                           <  1:1189338/91‑1 (MQ=255)
                                                                  gagagaATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGc                           <  2:2338404/100‑1 (MQ=255)
                                                                    gagaATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTg                         >  1:322741/1‑100 (MQ=255)
                                                                      gaATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCa                       >  2:698983/1‑100 (MQ=255)
                                                                       aaTGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCaa                      >  2:1995833/1‑100 (MQ=255)
                                                                         tGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAgg                    <  1:893729/100‑1 (MQ=255)
                                                                            cTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCa                 >  1:2054080/1‑100 (MQ=255)
                                                                            cTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCa                 <  2:2158362/100‑1 (MQ=255)
                                                                            cTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCa                 >  1:1752052/1‑100 (MQ=255)
                                                                            cTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCa                 >  1:1995634/1‑100 (MQ=255)
                                                                             tAAGGGGCAATCTTTACGAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCaa                <  2:90425/100‑1 (MQ=255)
                                                                                ggggCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATc             <  2:1138847/100‑1 (MQ=255)
                                                                                 nggCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGCTCCCGTATCTATTTATTTGGTTGATGGTATTAAACTGCATGGGCaaagtg            >  2:1294925/2‑97 (MQ=255)
                                                                                   gCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAg          <  2:169193/100‑1 (MQ=255)
                                                                                   gCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAg          <  1:825356/100‑1 (MQ=255)
                                                                                   gCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAg          <  1:1993881/100‑1 (MQ=255)
                                                                                   gCAATCTTTACAAGCCCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAg          <  2:64979/100‑1 (MQ=255)
                                                                                    cAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGt         <  2:1481338/100‑1 (MQ=255)
                                                                                    cAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGt         <  2:1240713/100‑1 (MQ=255)
                                                                                     aaTCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGAGTTCCAGTTTCTATTTATTTGGTGAAGGGTATTAAGCTGCAAGGGCAAATCGAGTc        >  2:18998/1‑100 (MQ=255)
                                                                                      agcTTTACAGGCTCCGTGCCTGAACGCACTGCGTCGGGCACGTGGTCCGGTTTCTCTTTATTTGGTGAATGGTATTAGGCTGCGAGGGCaaagag            >  2:545190/3‑92 (MQ=255)
                                                                                      aTCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCt       >  1:525382/1‑100 (MQ=255)
                                                                                        cTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCttt     >  1:1076060/1‑100 (MQ=255)
                                                                                        cTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCttt     >  2:1157349/1‑100 (MQ=255)
                                                                                        cTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCttt     >  2:1106662/1‑100 (MQ=255)
                                                                                         tttACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCtttt    >  1:1000177/1‑100 (MQ=255)
                                                                                         tttACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCtttt    >  1:1635749/1‑100 (MQ=255)
                                                                                         tttACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCtttt    <  2:1510956/100‑1 (MQ=255)
                                                                                         tttACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCtttt    >  2:1755490/1‑100 (MQ=255)
                                                                                          ttACAAGCTCCGTTCCTGAACGCGCTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTg   >  1:2239069/1‑100 (MQ=255)
                                                                                           tACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGa  <  1:2478428/100‑1 (MQ=255)
                                                                                           tACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGa  <  1:1013355/100‑1 (MQ=255)
                                                                                                 |                                                                                             
AGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGA  >  NC_000913/4400216‑4400406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A