Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F54 I0 R1 44 137.0 6550480 97.1% 6360516 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,951,598:1 +T 25.6% pseudogene (57/663 nt) ilvG → pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,951,5971.T25.6% 265.4 / 156.2 129pseudogene (56/663 nt)ilvGpseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted
Reads supporting (aligned to +/- strand):  ref base . (52/44);  new base T (14/19);  total (66/63)
Fisher's exact test for biased strand distribution p-value = 3.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.81e-01

Only 100 of 135 total aligned reads displayed.

TTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGAT  >  NC_000913/3951498‑3951696
                                                                                                    |                                                                                                   
tttAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCa                                                                                                      >  1:15854/1‑100 (MQ=255)
tttAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCa                                                                                                      >  2:1505042/1‑100 (MQ=255)
  tAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGAGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑Tc                                                                                                   >  2:412526/1‑100 (MQ=255)
    aaTGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑Tccc                                                                                                 >  2:807350/1‑100 (MQ=255)
      tGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCgg                                                                                               <  1:997374/100‑1 (MQ=255)
       gCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCgg                                                                                               <  1:1309986/100‑1 (MQ=255)
        ctctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTg                                                                                             <  2:2254512/100‑1 (MQ=255)
        ctctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTg                                                                                             >  2:203124/1‑100 (MQ=255)
        ctctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTg                                                                                             >  1:2075145/1‑100 (MQ=255)
         tctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTg                                                                                             <  1:1233261/100‑1 (MQ=255)
         tctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTg                                                                                             >  1:1753899/1‑100 (MQ=255)
          ctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGAc                                                                                           >  1:1081950/1‑100 (MQ=255)
          ctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGAc                                                                                           <  1:203126/100‑1 (MQ=255)
           tGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACg                                                                                          <  2:2508417/100‑1 (MQ=255)
            gTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGTCCA‑TCCCGGTGACGc                                                                                         <  1:3267050/100‑1 (MQ=255)
              tACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTa                                                                                       >  1:450258/1‑100 (MQ=255)
               aCCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTAt                                                                                      >  2:825264/1‑100 (MQ=255)
                ccAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCCACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTAt                                                                                      >  2:2022662/1‑100 (MQ=255)
                ccAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATc                                                                                     >  2:1713108/1‑100 (MQ=255)
                ccAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATc                                                                                     >  1:218946/1‑100 (MQ=255)
                     aTTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTAcgc                                                                                <  1:1557342/100‑1 (MQ=255)
                          agcagcCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCgt                                                                           <  1:2154185/100‑1 (MQ=255)
                          agcagcCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCgt                                                                           <  1:2520513/100‑1 (MQ=255)
                             agcCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATG‑‑CATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCgttgttg                                                                      <  1:1570026/100‑1 (MQ=255)
                              gcCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCgttgtt                                                                       >  1:2929166/1‑100 (MQ=255)
                              gcCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATG‑‑CATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCgttgttgt                                                                     >  1:1497742/1‑100 (MQ=255)
                              gcCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCgttgttg                                                                      <  2:2638629/100‑1 (MQ=255)
                               ccGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCgttgttg                                                                      <  1:314331/100‑1 (MQ=255)
                               ccGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCgttgttg                                                                      <  1:1254217/100‑1 (MQ=255)
                                cGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCgttgttgt                                                                     <  1:2617674/100‑1 (MQ=255)
                                cGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCgttgttg                                                                      >  1:2728617/1‑99 (MQ=255)
                                 gTTAAATCAACGACTGGCAGCAACACTGCGCGCCGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCgttgttg                                                                      <  2:3084015/98‑1 (MQ=255)
                                    aaaTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATG‑‑CATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAaac                                                               <  2:2293188/100‑1 (MQ=255)
                                     aaTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaaa                                                                 >  1:1675175/1‑100 (MQ=255)
                                     aaTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaaa                                                                 <  1:1836601/100‑1 (MQ=255)
                                      aTCAATGACTTGCAGCAACACTTCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCGATCTACGCGCCGTTGTTGTTAAaac                                                               <  2:1590001/100‑1 (MQ=255)
                                      aTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAaac                                                               <  2:1034260/100‑1 (MQ=255)
                                      aTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAaac                                                               <  2:2127396/100‑1 (MQ=255)
                                      aTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAaac                                                               >  2:683294/1‑100 (MQ=255)
                                       tCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAaac                                                               <  1:1719307/100‑1 (MQ=255)
                                       tCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAaaca                                                              >  1:764636/1‑100 (MQ=255)
                                             aCTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAACCAACTGTc                                                        >  2:257809/1‑100 (MQ=255)
                                             aCTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTc                                                        >  1:2510143/1‑100 (MQ=255)
                                              cTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTc                                                        <  2:2657546/100‑1 (MQ=255)
                                              cTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTTACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCg                                                       >  1:1685809/1‑100 (MQ=255)
                                              cTGGCAGCAACACTGCGCGCAGCTGCGTGATG‑‑CATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGa                                                     >  1:611787/1‑100 (MQ=255)
                                              cTGGCAGCAACACTGCGCGCAGCCGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTc                                                        <  2:1396257/100‑1 (MQ=255)
                                                ggCAGCAACACTGCGCGCCGATGCGTGATGAACATGCCTGACGTTATGAACA‑TCATGGTGAAGCTATCTACGCGCTGTTGTTGTTAAaacaac                                                            >  2:765292/1‑93 (MQ=255)
                                                ggCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGa                                                     <  1:1868796/100‑1 (MQ=255)
                                                  cagcaACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGCTc                                                   >  2:696348/1‑100 (MQ=255)
                                                  cagcaACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATc                                                   >  2:2232470/1‑100 (MQ=255)
                                                   agcaACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATc                                                   <  1:2930539/100‑1 (MQ=255)
                                                   agcaACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCg                                                  <  2:2677334/100‑1 (MQ=255)
                                                   agcaACACT‑‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTa                                                >  1:1223701/1‑100 (MQ=255)
                                                    gcaACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGt                                                 <  1:2484036/100‑1 (MQ=255)
                                                    gcaACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGt                                                 >  1:1532624/1‑100 (MQ=255)
                                                     caACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGt                                                 >  1:1197574/1‑100 (MQ=255)
                                                      aaCACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTaa                                               <  1:68620/100‑1 (MQ=255)
                                                       acacTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTaaa                                              >  2:1775618/1‑100 (MQ=255)
                                                          cTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCt                                           >  2:2781409/1‑100 (MQ=255)
                                                               cgcAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCg                                        <  2:2916881/99‑1 (MQ=255)
                                                                 cAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGAt                                     >  2:1873082/1‑100 (MQ=255)
                                                                  aGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTg                                   <  2:1175931/100‑1 (MQ=255)
                                                                   gCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTg                                   >  1:1395538/1‑100 (MQ=255)
                                                                   gCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGc                                  >  1:1353995/1‑100 (MQ=255)
                                                                   gCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGc                                  <  1:1324724/100‑1 (MQ=255)
                                                                   gCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCGATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGc                                  >  2:141713/1‑100 (MQ=255)
                                                                      gCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgt                                <  2:2694486/100‑1 (MQ=255)
                                                                      gCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtc                               >  1:2470638/1‑100 (MQ=255)
                                                                      gCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtc                               >  1:190068/1‑100 (MQ=255)
                                                                        gTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtcg                              <  1:1428637/100‑1 (MQ=255)
                                                                         tgatgaACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtcgt                             >  2:709083/1‑100 (MQ=255)
                                                                         tgatgaACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTg                            >  2:1337889/1‑100 (MQ=255)
                                                                          gatgaACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGa                           >  2:884626/1‑100 (MQ=255)
                                                                          gatgaACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGa                           <  2:2639531/100‑1 (MQ=255)
                                                                          gatgaACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGa                           >  1:279988/1‑100 (MQ=255)
                                                                           atgaACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGAc                          <  1:603639/100‑1 (MQ=255)
                                                                            tgaACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGAc                          <  2:249392/100‑1 (MQ=255)
                                                                            tgaACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGAcc                         <  2:1685805/100‑1 (MQ=255)
                                                                            tgaACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGAcc                         >  2:1447899/1‑100 (MQ=255)
                                                                            tgaACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGAcc                         <  2:1299611/100‑1 (MQ=255)
                                                                            tgaACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGAcc                         <  2:3229616/100‑1 (MQ=255)
                                                                             gaACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACca                        >  1:1140557/1‑100 (MQ=255)
                                                                              tgcATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACcac                       >  2:2161229/3‑100 (MQ=255)
                                                                              aaCATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGAcc                         <  2:367369/99‑1 (MQ=255)
                                                                              aaCATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACcac                       >  2:1053830/1‑100 (MQ=255)
                                                                               aCATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACcaca                      >  1:304699/1‑100 (MQ=255)
                                                                                cATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAg                     >  1:1008815/1‑100 (MQ=255)
                                                                                    ccTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGAtgt                 >  1:2895783/1‑100 (MQ=255)
                                                                                      tGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTgg               <  1:2355737/100‑1 (MQ=255)
                                                                                       ggCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTgg               <  1:709085/100‑1 (MQ=255)
                                                                                        gCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTggg              >  2:2728742/1‑100 (MQ=255)
                                                                                         cGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACGGATGTGGGgc            >  2:3124957/1‑100 (MQ=255)
                                                                                         cGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgc            <  1:2799442/100‑1 (MQ=255)
                                                                                            tACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTATAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgcag          <  2:2099172/99‑1 (MQ=255)
                                                                                             aCGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgcagca        >  1:1532046/1‑100 (MQ=255)
                                                                                              cGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgcagca        >  1:2424099/1‑100 (MQ=255)
                                                                                              cGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCAc       <  2:874646/100‑1 (MQ=255)
                                                                                                   a‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGAt  >  2:3070070/1‑100 (MQ=255)
                                                                                                   a‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGAt  <  1:1811992/100‑1 (MQ=255)
                                                                                                    |                                                                                                   
TTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGAT  >  NC_000913/3951498‑3951696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A