Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F54 I0 R1	44	137.0	6550480	97.1%	6360516	99.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,573,528	A→G	100%	pseudogene (1115/1503 nt)	yjiT →	pseudogene

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,573,528	0	A	G	99.3%	470.2 / ‑4.6	131	pseudogene (1115/1503 nt)	yjiT	pseudogene
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (60/70); total (60/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

Only 100 of 134 total aligned reads displayed.

AAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGACAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAAGCAG  >  NC_000913/4573432‑4573624

aaaGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAg                                                                                               <  1:388422/100‑1 (MQ=255)

aaaGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAg                                                                                               <  1:2571732/100‑1 (MQ=255)

  aGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTg                                                                                             <  1:167331/100‑1 (MQ=255)

  aGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTg                                                                                             >  2:2003406/1‑100 (MQ=255)

   gCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGa                                                                                            >  1:755694/1‑100 (MQ=255)

     gAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGaaa                                                                                          <  2:2243069/100‑1 (MQ=255)

     gAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGaaa                                                                                          <  1:2760303/100‑1 (MQ=255)

         tGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGCTGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTg                                                                                       >  2:3064352/1‑99 (MQ=255)

          gagaTTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGcc                                                                                     >  2:694056/1‑100 (MQ=255)

          gagaTTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGcc                                                                                     >  2:1034669/1‑100 (MQ=255)

          gagaTTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGcc                                                                                     <  1:1707739/100‑1 (MQ=255)

            gaTTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCAt                                                                                   >  1:2542870/1‑100 (MQ=255)

                tGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGt                                                                               >  2:2682127/1‑100 (MQ=255)

                 ggcaggcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTg                                                                              >  2:1033086/1‑100 (MQ=255)

                   caggcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGCGAg                                                                            <  1:1391425/100‑1 (MQ=255)

                    aggcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAgg                                                                           <  2:1476902/100‑1 (MQ=255)

                     ggcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGt                                                                          <  1:1043930/100‑1 (MQ=255)

                     ggcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGt                                                                          >  2:265504/1‑100 (MQ=255)

                      gcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTg                                                                         <  1:478487/100‑1 (MQ=255)

                       caAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGc                                                                        <  1:2055673/100‑1 (MQ=255)

                           aTCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGtt                                                                    <  2:1182978/100‑1 (MQ=255)

                           aTCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGtt                                                                    >  1:16810/1‑100 (MQ=255)

                             ccATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTaa                                                                  <  2:1343022/100‑1 (MQ=255)

                              cATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATTGCAGTGAAATTGCCATTGGTGAGGTGCCGTTaa                                                                  <  2:1960672/99‑1 (MQ=255)

                               aTCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAAcc                                                                <  1:265506/100‑1 (MQ=255)

                               aTCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAAcc                                                                <  2:2883262/100‑1 (MQ=255)

                               aTCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAAcc                                                                <  1:2990881/100‑1 (MQ=255)

                                tCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCt                                                               <  1:1475257/100‑1 (MQ=255)

                                 cGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCtt                                                              <  1:2330337/100‑1 (MQ=255)

                                 cGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCtt                                                              >  2:2678566/1‑100 (MQ=255)

                                  ggCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTc                                                             <  2:1550135/100‑1 (MQ=255)

                                  ggCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTc                                                             <  1:469455/100‑1 (MQ=255)

                                     aGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATc                                                          <  2:2351942/100‑1 (MQ=255)

                                      ggTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCg                                                         >  1:3004419/1‑100 (MQ=255)

                                       gTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGt                                                        >  1:305434/1‑100 (MQ=255)

                                        tttGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGtt                                                       <  1:784177/100‑1 (MQ=255)

                                        tttGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGtt                                                       <  2:505331/100‑1 (MQ=255)

                                         ttGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTg                                                      <  2:2962194/100‑1 (MQ=255)

                                         ttGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTg                                                      <  2:1253306/100‑1 (MQ=255)

                                         ttGGCGTTAGTGGCTCGGGCTTGAGGGATTATTGTTGGGATCATTAAACTTGATGACAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTg                                                      <  1:3058475/100‑1 (MQ=255)

                                          tGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGa                                                     >  1:2635148/1‑100 (MQ=255)

                                           gTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGAt                                                    >  2:2972078/1‑100 (MQ=255)

                                           gTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCAGTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGAt                                                    <  1:205436/100‑1 (MQ=255)

                                           gTCGTTAGTGGCGCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGAt                                                    <  2:1772520/100‑1 (MQ=255)

                                            tCGTTAGTAGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATg                                                   <  2:1772343/100‑1 (MQ=255)

                                             cGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGc                                                  <  1:413964/100‑1 (MQ=255)

                                               ttAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAg                                                >  1:195953/1‑100 (MQ=255)

                                                tAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGa                                               >  2:882204/1‑100 (MQ=255)

                                                tAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGa                                               <  1:1339101/100‑1 (MQ=255)

                                                  gTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATc                                             <  2:1012959/100‑1 (MQ=255)

                                                  gTGGCGCGTGCTGGAGGGTTGACTGTTGGGAGCATTAAACTTGATGGCAGTGAAATGGTCGTTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCGGAt                                              >  2:59754/1‑99 (MQ=255)

                                                      cTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATg                                         <  2:305431/100‑1 (MQ=255)

                                                      cTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATg                                         <  1:3013703/100‑1 (MQ=255)

                                                        cGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGc                                       >  2:554820/1‑100 (MQ=255)

                                                         gTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCt                                      <  1:1314161/100‑1 (MQ=255)

                                                          tGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTg                                     >  2:3142205/1‑100 (MQ=255)

                                                          tGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTg                                     >  1:2444009/1‑100 (MQ=255)

                                                           gCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGt                                    >  1:1443046/1‑100 (MQ=255)

                                                            cTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGtt                                   >  1:3226100/1‑100 (MQ=255)

                                                             tGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCTTTGATGCAGATCAATGGCTGTTa                                  <  2:3214346/100‑1 (MQ=255)

                                                              ggAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTAc                                 >  1:2085558/1‑100 (MQ=255)

                                                               gAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACa                                >  2:2821853/1‑100 (MQ=255)

                                                               gAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACa                                >  1:220596/1‑100 (MQ=255)

                                                                 gggATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAgg                              >  1:1267691/1‑100 (MQ=255)

                                                                 gggATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAgg                              >  1:2909760/1‑100 (MQ=255)

                                                                 gggATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAgg                              >  2:2331015/1‑100 (MQ=255)

                                                                  ggATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAggg                             >  1:3075308/1‑100 (MQ=255)

                                                                   tctgatTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGa                            <  2:433407/98‑1 (MQ=255)

                                                                   gatgatTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGa                            >  1:1598013/1‑100 (MQ=255)

                                                                   gatgatTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGa                            >  1:2339707/1‑100 (MQ=255)

                                                                   gatgatTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGa                            <  2:2600371/100‑1 (MQ=255)

                                                                    atgatTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGAc                           <  1:2616287/100‑1 (MQ=255)

                                                                     tgatTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACa                          >  2:2648730/1‑100 (MQ=255)

                                                                      gatTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGaaga                         >  1:433409/1‑97 (MQ=255)

                                                                      gatTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAg                         <  2:1226169/100‑1 (MQ=255)

                                                                       atTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAgg                        >  1:1083262/1‑100 (MQ=255)

                                                                         tgttgGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGct                      >  2:1193924/1‑100 (MQ=255)

                                                                         tgttgGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGct                      <  1:3260834/100‑1 (MQ=255)

                                                                          gttgGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGctt                     >  1:1238051/1‑100 (MQ=255)

                                                                           ttgGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGcttc                    <  2:635128/100‑1 (MQ=255)

                                                                           ttgGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGcttc                    <  2:376347/100‑1 (MQ=255)

                                                                             gggAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGcttctt                  >  1:2966120/1‑100 (MQ=255)

                                                                              ggAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTg                 <  1:2873716/100‑1 (MQ=255)

                                                                               gAGCATTAAGCTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGc                <  2:2689499/100‑1 (MQ=255)

                                                                               gAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGc                <  2:647510/100‑1 (MQ=255)

                                                                                 gCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAg              >  1:321368/1‑100 (MQ=255)

                                                                                 gCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAg              >  1:2070991/1‑100 (MQ=255)

                                                                                   aTTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAgtg            <  1:238213/100‑1 (MQ=255)

                                                                                   aTTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAgtg            >  1:1566651/1‑100 (MQ=255)

                                                                                    ttAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAgtgt           >  1:1122852/1‑100 (MQ=255)

                                                                                     tAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGtgtg          >  2:1191011/1‑100 (MQ=255)

                                                                                     tAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGtgtg          <  2:12817/100‑1 (MQ=255)

                                                                                      aaaCTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGc         >  2:2359301/1‑100 (MQ=255)

                                                                                      aaaCTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGc         <  2:2734156/100‑1 (MQ=255)

                                                                                       aaCTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCg        <  1:636852/100‑1 (MQ=255)

                                                                                         cTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGaa      >  1:3084937/1‑100 (MQ=255)

                                                                                         cTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGaa      >  1:3085218/1‑100 (MQ=255)

                                                                                           tgatggCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAagc    >  2:2788607/1‑100 (MQ=255)

                                                                                           tgatggCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATCGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAagc    >  2:2213340/1‑100 (MQ=255)

                                                                                             atggCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAagcag  >  2:849287/1‑100 (MQ=255)

AAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGACAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAAGCAG  >  NC_000913/4573432‑4573624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A