Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F125 I1 R1 45 113.1 5779376 92.5% 5345922 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,059,320 C→T P305L (CCG→CTG)  typA → GTP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,059,3200CT97.6% 287.4 / ‑2.2 83P305L (CCG→CTG) typAGTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base T (42/39);  total (44/39)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01

CGTTGCGATCACGGGCCTTGGCGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCCGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGTTCGTAACGTCTCGTCAGATCCTGGATCGTCTGAACAA  >  NC_000913/4059225‑4059416
                                                                                               |                                                                                                
cGTTGCGATCACGGGCCTTGGCGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGAcc                                                                                              >  2:1541915/1‑100 (MQ=255)
cGTTGCGATCACGGGCCTTGGCGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGAcc                                                                                              >  2:1630863/1‑100 (MQ=255)
cGTTGCGATCACGGGCCTTGGCGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGAcc                                                                                              <  1:606798/100‑1 (MQ=255)
cGTTGCGATCACGGGCCTTGGCGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAACCGCTGCCGGCACTCTCCGTTGATGAGCTGAcc                                                                                              <  2:2737797/100‑1 (MQ=255)
 gTTGCGATCACGGGCCTTGGCGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCg                                                                                             >  1:1865223/1‑100 (MQ=255)
   tGCGATCACGGGCCTTGGCGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGtt                                                                                           >  2:452250/1‑100 (MQ=255)
   tGCGATCACGGGCCTTGGCGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGtt                                                                                           >  2:2164305/1‑100 (MQ=255)
    gCGATCACGGGCCTTGGCGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGttt                                                                                          >  1:981213/1‑100 (MQ=255)
     cGATCACGGGCCTTGGCGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTc                                                                                         >  1:1168448/1‑100 (MQ=255)
     cGATCACGGGCCTTGGCGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTc                                                                                         <  1:2646198/100‑1 (MQ=255)
      gATCACGGGCCTTGGCGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCt                                                                                        >  2:1710078/1‑100 (MQ=255)
      gATCACGGGCCTTGGCGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCt                                                                                        <  1:1857457/100‑1 (MQ=255)
       aTCACGGGCCTTGGCGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTa                                                                                       <  2:789570/100‑1 (MQ=255)
       aTCACGGGCCTTGGCGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCATTTCTa                                                                                       <  2:2692344/100‑1 (MQ=255)
          aCGGGCCTTGGCGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATg                                                                                     <  1:2556162/99‑1 (MQ=255)
           cGGGCCTTGGCGAATTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGtt                                                                                   <  1:538363/100‑1 (MQ=255)
           cGGGCCTTGGCGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGtt                                                                                   >  2:2205517/1‑100 (MQ=255)
           cGGGCCTTGGCGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGtt                                                                                   <  2:1645904/100‑1 (MQ=255)
             ggCCTTGGCGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGttct                                                                                 <  2:2217293/100‑1 (MQ=255)
             ggCCTTGGCGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGttct                                                                                 >  2:24055/1‑100 (MQ=255)
             ggCCTTGGCGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGttct                                                                                 >  2:840260/1‑100 (MQ=255)
             ggCCTTGGCGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGttct                                                                                 >  2:1598763/1‑100 (MQ=255)
               ccTTGGCGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCCGACCGTTTCTATGttcttc                                                                               >  1:1420903/1‑100 (MQ=255)
                   ggCGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCg                                                                           >  2:2316246/1‑100 (MQ=255)
                   ggCGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCg                                                                           >  2:2720089/1‑100 (MQ=255)
                     cGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGtt                                                                         <  2:1824181/100‑1 (MQ=255)
                     cGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGtt                                                                         <  1:1397553/100‑1 (MQ=255)
                     cGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGtt                                                                         <  1:1119325/100‑1 (MQ=255)
                      gAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTa                                                                        <  1:946990/100‑1 (MQ=255)
                      gAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTa                                                                        >  2:946993/1‑100 (MQ=255)
                        aCTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAac                                                                      >  2:146574/1‑100 (MQ=255)
                        aCTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAac                                                                      <  2:1894690/100‑1 (MQ=255)
                        aCTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAac                                                                      <  2:981217/100‑1 (MQ=255)
                        aCTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAac                                                                      >  1:2705403/1‑100 (MQ=255)
                          tGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAacac                                                                    >  1:1498219/1‑100 (MQ=255)
                           gAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACAcc                                                                   >  1:1306434/1‑100 (MQ=255)
                              cATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCg                                                                >  2:1771854/1‑100 (MQ=255)
                              cATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCg                                                                >  2:892252/1‑100 (MQ=255)
                                tttCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGcc                                                              >  2:1850609/1‑100 (MQ=255)
                                 ttCTGACACTCTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCg                                                             <  2:2045550/100‑1 (MQ=255)
                                 ttCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTCCTTCTGCGTTAACACCTCGCCg                                                             >  1:1960452/1‑100 (MQ=255)
                                       cacCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGc                                                       <  1:1328718/100‑1 (MQ=255)
                                       cacCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCGCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGc                                                       <  1:1850596/100‑1 (MQ=255)
                                        acCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCg                                                      <  2:2795286/100‑1 (MQ=255)
                                        acCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCg                                                      >  2:2107172/1‑100 (MQ=255)
                                         ccGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCgg                                                     <  1:594965/100‑1 (MQ=255)
                                         ccGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCgg                                                     <  2:2198177/100‑1 (MQ=255)
                                           gTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTa                                                   >  1:1102706/1‑100 (MQ=255)
                                                cGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAaagaa                                              >  2:486710/1‑100 (MQ=255)
                                                cGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCCGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAaagaa                                              >  1:2671588/1‑100 (MQ=255)
                                                     cGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTaa                                         >  2:2369638/1‑100 (MQ=255)
                                                     cGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTa                                          <  2:326246/99‑1 (MQ=255)
                                                      gCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAg                                        <  2:1715847/100‑1 (MQ=255)
                                                       cAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGt                                       <  2:1773095/100‑1 (MQ=255)
                                                       cAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGt                                       >  1:2426062/1‑100 (MQ=255)
                                                        aaaaCGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGtt                                      <  1:2331897/100‑1 (MQ=255)
                                                         aaaCGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGTTc                                     >  2:1428804/1‑100 (MQ=255)
                                                            cGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGTTCGTa                                  <  1:2158243/100‑1 (MQ=255)
                                                              ttGAAGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGTTCGTAAc                                >  1:842552/1‑100 (MQ=255)
                                                                 aaGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGTTCGTAACGtc                             >  2:1770878/1‑100 (MQ=255)
                                                                  aGCGCTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGTTCGTAACGtct                            <  1:2720065/100‑1 (MQ=255)
                                                                   gcgcTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGTTCGTAACGtctc                           >  2:64059/1‑100 (MQ=255)
                                                                    cgcTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGTTCGTAACGTCTCg                          >  2:1048194/1‑100 (MQ=255)
                                                                      cTGCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGTTCGTAACGTCTCGTc                        <  1:617731/100‑1 (MQ=255)
                                                                        gCCGGCACTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGTTCGTAACGTCTCGTCAg                      >  2:2090729/1‑100 (MQ=255)
                                                                              aCTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGTTCGTAACGTCTCGTCAGATCCTg                <  2:897817/100‑1 (MQ=255)
                                                                              aCTCTCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGTTCGTAACGTCTCGTCAGATCCTg                <  2:1306324/100‑1 (MQ=255)
                                                                               ctctCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGTTCGTAACGTCTCGTCAGATCCTgg               >  1:1361907/1‑100 (MQ=255)
                                                                               ctctCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGTTCGTAACGTCTCGTCAGATCCTgg               <  2:252633/100‑1 (MQ=255)
                                                                               ctctCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGTTCGTAACGTCTCGTCAGATCCTgg               <  1:719541/100‑1 (MQ=255)
                                                                               ctctCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGTTCGTAACGTCTCGTCAGATCCTg                <  1:2021283/99‑1 (MQ=255)
                                                                               ctctCCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGTTCGTAACGTCTCGTCAGATCCTg                <  1:314724/99‑1 (MQ=255)
                                                                                 ctcCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGTTCGTAACGTCTCGTCAGATCCTGGAt             >  1:2076939/1‑100 (MQ=255)
                                                                                 ctcCGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGTTCGTAACGTCTCGTCAGATCCTGGAt             >  2:335617/1‑100 (MQ=255)
                                                                                   ccGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGTTCGTAACGTCTCGTCAGATCCTGGATCg           >  1:1624304/1‑100 (MQ=255)
                                                                                   ccGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGTTCGTAACGTCTCGTCAGATCCTGGATCg           >  2:684204/1‑100 (MQ=255)
                                                                                   ccGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGTTCGTAACGTCTCGTCAGATCCTGGATCg           >  2:268542/1‑100 (MQ=255)
                                                                                   ccGTTGATGAGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGTTCGTAACGTCTCGTCAGATCCTGGATCg           >  1:1019761/1‑100 (MQ=255)
                                                                                       tgatgaGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGTTCGTAACGTCTCGTCAGATCCTGGATCGTCTg       <  1:2316228/100‑1 (MQ=255)
                                                                                        gatgaGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCGCGCCGTTCTGCGGTAAAGAAGGTAAGTTCGTAACGTCTCGTCAGATCCTGGATCGTCTGa      <  2:2705427/100‑1 (MQ=255)
                                                                                          tgaGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGTTCGTAACGTCTCGTCAGATCCTGGATCGTCTGAAc    <  2:2601338/100‑1 (MQ=255)
                                                                                          tgaGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGTTCGTAACGTCTCGTCAGATCCTGGATCGTCTGAAc    <  1:24055/100‑1 (MQ=255)
                                                                                            aGCTGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGTTCGTAACGTCTCGTCAGATCCTGGATCGTCTGAACaa  >  2:2452932/1‑100 (MQ=255)
                                                                                               |                                                                                                
CGTTGCGATCACGGGCCTTGGCGAACTGAACATTTCTGACACCGTTTGCGACACGCAAAACGTTGAAGCGCTGCCGGCACTCTCCGTTGATGAGCCGACCGTTTCTATGTTCTTCTGCGTTAACACCTCGCCGTTCTGCGGTAAAGAAGGTAAGTTCGTAACGTCTCGTCAGATCCTGGATCGTCTGAACAA  >  NC_000913/4059225‑4059416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A