Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F232 I0 R1 243 156.3 7646460 93.8% 7172379 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,202,685 A→G 16.6% E265E (GAA→GAG)  cca → fused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,202,6850AG16.6% 430.6 / 78.2 193E265E (GAA→GAG) ccafused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase
Reads supporting (aligned to +/- strand):  ref base A (88/72);  new base G (16/16);  total (105/88)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.58e-03

Only 100 of 201 total aligned reads displayed.

CTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCC  >  NC_000913/3202586‑3202784
                                                                                                   |                                                                                                   
cTTAATGACGCTCTCAATGGCGGCGATGGTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGaa                                                                                                     <  1:709720/100‑1 (MQ=255)
  tAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAAct                                                                                                   >  1:267130/1‑100 (MQ=255)
   aaTGTCGCTCCCAGTGGTGGCTATGCTGAGTCCTCACTTCGAGATCCGTTTCGCAACGTTATGCCTCGATCTCGGTAAAGGGCTGACGCCGCCAGAActc                                                                                                  <  2:1064294/100‑1 (MQ=255)
     tGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTg                                                                                                >  2:625282/1‑100 (MQ=255)
      gACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTgg                                                                                               <  1:2907478/100‑1 (MQ=255)
      gACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTgg                                                                                               <  1:3500197/100‑1 (MQ=255)
        cGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGcc                                                                                             >  2:1355386/1‑100 (MQ=255)
        cGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGcc                                                                                             >  1:1284902/1‑100 (MQ=255)
        cGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGcc                                                                                             <  1:3638027/100‑1 (MQ=255)
         gCTCTCAATGGCAGCGATGCTGAGTCCGCAGGTCGATGTCCGTCTCGCAACTTGATGCCACGAGCTCGGTAAAGGGCTGACGCCGCCAGAACTCTgg                                                                                               <  2:1012762/97‑1 (MQ=255)
          ctctCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCcgc                                                                                           >  2:1580875/1‑100 (MQ=255)
          ctctCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCcgc                                                                                           >  1:821863/1‑100 (MQ=255)
           tctcAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGgcggcg                                                                                          <  1:3802358/100‑1 (MQ=255)
           tctcAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCcgcg                                                                                          <  2:1745415/100‑1 (MQ=255)
             tcAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtc                                                                                        >  1:1441140/1‑100 (MQ=255)
             tcAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtc                                                                                        >  1:2804068/1‑100 (MQ=255)
             tcAATGGCGGCGATGCTGAGTCCGCAGGTCGATGCCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtc                                                                                        <  1:90397/100‑1 (MQ=38)
                acggcggcGATCCTAAGTCCGCAGGTCGATGTCCCTTTCGCAACTTTATGCCACGATCGCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagc                                                                                     <  2:3074051/98‑3 (MQ=25)
                aTGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagc                                                                                     <  1:3014763/100‑3 (MQ=38)
                aTGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtcatc                                                                                     >  2:1761381/1‑100 (MQ=255)
                aTGGCGGCGATGCGGAGTCCGCAGGTGGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagc                                                                                     <  2:570116/100‑3 (MQ=37)
                 tGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtcatca                                                                                    <  1:580910/100‑1 (MQ=255)
                 tGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTACGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtcatc                                                                                     <  1:3778641/99‑1 (MQ=255)
                  ggcggcGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTcatcat                                                                                   >  2:1283618/1‑100 (MQ=255)
                    cggcgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCCGATGCCGCCAGAACTCTGGCCGCGTCAtcatgg                                                                                 <  2:2508367/100‑1 (MQ=255)
                     ggcgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggt                                                                                <  1:1288227/100‑1 (MQ=255)
                       cgATGCTGAGTCCTCAGGTCGATGTCCTTTTCGCCACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggt                                                                                <  2:1868714/98‑1 (MQ=255)
                       cgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggtc                                                                               >  2:1693389/1‑99 (MQ=255)
                       cgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggtc                                                                               >  1:325753/1‑99 (MQ=255)
                          tGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggtcatgg                                                                           <  1:330954/100‑1 (MQ=255)
                           gCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGc                                                                          >  2:3358575/1‑100 (MQ=255)
                            cTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGcc                                                                         >  2:3206107/1‑100 (MQ=255)
                            cTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGcc                                                                         >  2:2773028/1‑100 (MQ=255)
                             tGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGcc                                                                         >  2:1181212/1‑99 (MQ=255)
                              gattCCGCAGGTTTAGGAGCGGTTAGCGACTCTATGCCGCGAGCTCGGTAAAGGGCTGACGTCGCCAGAACTCTGGCCGCTTCAtcatggtcatgg                                                                           <  2:349303/93‑1 (MQ=255)
                                   cGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCggg                                                                  <  1:1414454/100‑1 (MQ=255)
                                   cGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCggg                                                                  >  1:1071470/1‑100 (MQ=255)
                                     cAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGgtg                                                                <  1:1641462/100‑1 (MQ=255)
                                      aGGTCGATGCCCGTATCGCAAATTTATAAACCGATCTCGGTAAAGCGCTGAAGACGACAGAACTCTGGCAGAATCATCATGGTCATGGCCCGGAGGgtg                                                                <  2:1128433/99‑1 (MQ=255)
                                        gTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTa                                                             <  2:2829017/100‑1 (MQ=255)
                                             tGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTa                                                        >  2:3053146/1‑100 (MQ=255)
                                              gTCCGTTTCGCACCTTTATGCCACGACCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAg                                                       >  1:413204/1‑100 (MQ=255)
                                              gTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAg                                                       >  2:3327876/1‑100 (MQ=255)
                                              gTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACGCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGtt                                                         <  2:3262264/98‑1 (MQ=255)
                                                ccGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTg                                                     <  1:218339/100‑1 (MQ=255)
                                                ccGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTg                                                     <  1:467111/100‑1 (MQ=255)
                                                 cGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTgg                                                    >  1:54928/1‑100 (MQ=255)
                                                 cGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTgg                                                    <  1:640065/100‑1 (MQ=255)
                                                    ttCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGaac                                                 <  1:981675/100‑1 (MQ=255)
                                                     tCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCCTGGCCCGGCGGGGGTTAAGTTAGTGGaaca                                                >  2:2063420/1‑100 (MQ=255)
                                                      cGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGaacaa                                               >  2:3388331/1‑100 (MQ=255)
                                                      cGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCACGGCGGGTGTTAAGTTAGTGGaacaa                                               >  1:2299016/1‑100 (MQ=255)
                                                       gCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGAGTTAAGTTAGTGGaacaac                                              >  2:3656642/1‑100 (MQ=255)
                                                        cAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACt                                             >  1:2043183/1‑100 (MQ=255)
                                                        cAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACt                                             >  1:1891932/1‑100 (MQ=255)
                                                        cAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACt                                             >  2:2601166/1‑100 (MQ=255)
                                                           cTTTATTCCACGATCTCCGTAAAGCGCTGACGCCCCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATg                                          <  1:3460699/100‑1 (MQ=255)
                                                           cTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATg                                          <  1:39357/100‑1 (MQ=255)
                                                             ttATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGcc                                        <  1:1026421/100‑1 (MQ=255)
                                                               aTGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAg                                      <  1:2482483/100‑1 (MQ=255)
                                                                 gCCACGGTCTCGGTAAAGGGCTGACGCCGCCAGAACTCTTGCCGCGGCATCATGGTCATGGCAAGCCGGGAGCTAAGTTAGAGGAACACCTATGCCa                                       >  2:712679/1‑97 (MQ=255)
                                                                 gCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCg                                    >  1:2072723/1‑100 (MQ=255)
                                                                 gCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCg                                    >  2:3449218/1‑100 (MQ=255)
                                                                   cACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTc                                  <  1:2550279/100‑1 (MQ=255)
                                                                      gATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTgc                               >  1:1139632/1‑100 (MQ=255)
                                                                        tctcGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTACTGGAACAACTATGCCAGCGTCTgcgc                             >  2:2966980/1‑100 (MQ=255)
                                                                        tctcGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGATCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTCTGCCAGCGTCTgcgc                             >  2:1822387/1‑100 (MQ=255)
                                                                         tctggTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGcgcg                            <  2:2151943/97‑1 (MQ=255)
                                                                         ctcGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTgcgc                             >  2:2494254/1‑99 (MQ=255)
                                                                           cGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTg                          <  1:1552580/100‑1 (MQ=255)
                                                                            ggTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGcgcgaga                         >  1:2151957/1‑97 (MQ=255)
                                                                             gTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGcc                        <  2:3749461/100‑1 (MQ=255)
                                                                              tAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCa                       <  1:1761391/100‑1 (MQ=255)
                                                                               aaaGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCaa                      <  1:1184712/100‑1 (MQ=255)
                                                                                aaGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCaaa                     >  2:1987585/1‑100 (MQ=255)
                                                                                 cgatcTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAAt                    <  1:3366454/96‑1 (MQ=255)
                                                                                 catgatGACGCGGCCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCaaa                     >  2:817791/6‑99 (MQ=37)
                                                                                 catgatGACGCGGCCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCATCTGCGCGTGCCaaa                     >  1:20771/6‑99 (MQ=25)
                                                                                 aGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAAt                    >  1:1245772/1‑100 (MQ=255)
                                                                                   ggCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGa                  >  2:363465/1‑100 (MQ=255)
                                                                                   ggCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGa                  <  1:86435/100‑1 (MQ=255)
                                                                                    gCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTCAGTTAGTGGAACAACGATGCCAGCGTCTGCGCGTGCCAAAGGa                  >  2:3407108/1‑99 (MQ=255)
                                                                                    gCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGaa                 >  1:1352012/1‑100 (MQ=255)
                                                                                     cTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGaaa                <  1:3157911/100‑1 (MQ=255)
                                                                                      tGACGCGGCCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAAt               >  2:2303513/1‑100 (MQ=38)
                                                                                       gACGCGGCCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAAtt              >  2:2759870/1‑100 (MQ=38)
                                                                                       gACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAAtagatcg              >  2:3366428/1‑94 (MQ=255)
                                                                                        aCGCGGCCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTc             >  1:2732569/1‑100 (MQ=38)
                                                                                         cgcggcCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcg            >  2:2190069/1‑100 (MQ=255)
                                                                                         cgcggcCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcg            >  2:2128198/1‑100 (MQ=255)
                                                                                         cgcggcCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcg            >  1:3681525/1‑100 (MQ=255)
                                                                                         cgcggcCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcg            >  1:1578635/1‑100 (MQ=255)
                                                                                          gccgccAGAACTCTGGCCGCATCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcgc           <  1:3409536/100‑1 (MQ=255)
                                                                                            cgccAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGa         >  1:725441/1‑100 (MQ=255)
                                                                                            cgccAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGa         <  1:1824797/100‑1 (MQ=255)
                                                                                            cgccAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCACCGTCTGCGCGTGCCAAATGAAATTcgcg          >  2:3620011/1‑99 (MQ=255)
                                                                                              ccAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGAtt       <  2:539828/100‑1 (MQ=255)
                                                                                                  aaCTCTGGCCGCGTCATCATAATCACAGCCCGGCGTCTGTTAAGTTAGTGGAACAGCTATGTCAGCGCCTGTGCGTGTCAAATTAAATTCGCGATTTTGc   <  2:877995/100‑1 (MQ=255)
                                                                                                  aaCTCCGCCCGCGTCATCATGGTCATAGCCCGTCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTa     <  2:1787117/98‑1 (MQ=255)
                                                                                                   aCTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGcc  <  1:3121009/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
CTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCC  >  NC_000913/3202586‑3202784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A