Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F232 I1 R1 72 114.8 5816246 94.5% 5496352 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,453,105 G→A H56Y (CAC→TAC)  rpsJ ← 30S ribosomal subunit protein S10

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,453,1050GA98.2% 367.5 / ‑3.2 111H56Y (CAC→TAC) rpsJ30S ribosomal subunit protein S10
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (56/53);  total (57/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 111 total aligned reads displayed.

CAGAGCATCAACGGTTTTCTCGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTC  >  NC_000913/3453010‑3453201
                                                                                               |                                                                                                
cAGAGCATCAACGGTTTTCTCGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACggg                                                                                              >  2:2625548/1‑100 (MQ=255)
   agCATCAACGGTTTTCTCGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGgag                                                                                           <  2:700544/100‑1 (MQ=255)
      aTCAACGGTTTTCTCGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATc                                                                                        <  2:160566/100‑1 (MQ=255)
      aTCAACGGTTTTCTCGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATc                                                                                        <  1:1277062/100‑1 (MQ=255)
      aTCAACGGTTTTCTCGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATc                                                                                        <  1:317453/100‑1 (MQ=255)
       tCAACGGTTTTCTCGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCa                                                                                       <  2:2451935/100‑1 (MQ=255)
        cAACGGTTTTCTCGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAg                                                                                      >  2:1668941/1‑100 (MQ=255)
        cAACGGTTTTCTCGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAg                                                                                      >  2:1545755/1‑100 (MQ=255)
        cAACGGTTTTCTCGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAg                                                                                      >  2:1517066/1‑100 (MQ=255)
        cAACGGTTTTCTCGGTTGGCTCAACGACGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAg                                                                                      <  1:2839988/100‑1 (MQ=255)
         atcGGTTTTCTCGGTTGGCTCAACTGTGTGAACCAGACGCATGTGAGTACGGATTTGGTACTGATCGCGCGCGTCTTTGTTGAAGTGCGGGGAGATCAGa                                                                                     <  1:2543384/98‑1 (MQ=255)
         aaCGGTTTTCTCGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGa                                                                                     >  1:2256032/1‑100 (MQ=255)
         aaCGGTTTTCTCGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGa                                                                                     >  2:2305691/1‑100 (MQ=255)
         aaCGGTTTTCTCGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGa                                                                                     <  1:55933/100‑1 (MQ=255)
         aaCGGTTTTCTCGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGa                                                                                     >  2:95610/1‑100 (MQ=255)
           cGGTTTTCTCGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAAc                                                                                   <  2:1423387/100‑1 (MQ=255)
           cGGTTTTCTCGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAAc                                                                                   <  1:1944403/100‑1 (MQ=255)
           cGGTTTTCTCGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAAc                                                                                   <  1:2328961/100‑1 (MQ=255)
               tttCTCGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTg                                                                               <  1:1672134/100‑1 (MQ=255)
                ttCTCGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTg                                                                               >  2:1143788/1‑99 (MQ=255)
                 tctcGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGa                                                                              >  2:1472549/1‑99 (MQ=255)
                  ctcGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAg                                                                            >  1:2220123/1‑100 (MQ=255)
                  ctcGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAg                                                                            <  1:1538298/100‑1 (MQ=255)
                  ctcGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAg                                                                            <  2:334138/100‑1 (MQ=255)
                  ctcGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAg                                                                            >  1:2478967/1‑100 (MQ=255)
                  ctcGGTTGGCTCAACGATGTCAACCAGACGCAAGCGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAg                                                                            >  2:2027511/1‑100 (MQ=255)
                   tcGGTGGGCTCAACGATGTCAACCTGACGCAAGTGATTACGGATTTCGTTCTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAgc                                                                           <  2:2630246/100‑1 (MQ=255)
                     ggTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAgcgc                                                                         <  2:2832188/100‑1 (MQ=255)
                          gCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCttt                                                                    >  2:14303/1‑100 (MQ=255)
                           cTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTg                                                                   >  2:967029/1‑100 (MQ=255)
                           cTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTg                                                                   >  2:322519/1‑100 (MQ=255)
                           cTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTg                                                                   >  1:1413281/1‑100 (MQ=255)
                             cAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCg                                                                 >  2:165064/1‑100 (MQ=255)
                             cAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCg                                                                 >  2:2033083/1‑100 (MQ=255)
                             cAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCg                                                                 >  2:2196145/1‑100 (MQ=255)
                               aCGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCgtg                                                               >  1:1498270/1‑100 (MQ=255)
                                cGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCgtgt                                                              >  2:1265486/1‑100 (MQ=255)
                                  aTGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCg                                                            <  1:1095018/100‑1 (MQ=255)
                                   tGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCgg                                                           >  2:2507618/1‑100 (MQ=255)
                                   tGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCgg                                                           >  1:1142506/1‑100 (MQ=255)
                                    gTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGc                                                          <  2:2078870/100‑1 (MQ=255)
                                    gTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGc                                                          >  2:1302558/1‑100 (MQ=255)
                                    gTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGc                                                          >  1:458480/1‑100 (MQ=255)
                                      cAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAg                                                        >  1:351389/1‑100 (MQ=255)
                                      cAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAg                                                        >  1:511539/1‑100 (MQ=255)
                                      cAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAg                                                        >  1:1413626/1‑100 (MQ=255)
                                        aCCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCg                                                      <  1:2196118/100‑1 (MQ=255)
                                         ccAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCgg                                                     <  2:300565/100‑1 (MQ=255)
                                         ccAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCgg                                                     <  1:1433198/100‑1 (MQ=255)
                                             aCGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATc                                                 <  2:271561/100‑1 (MQ=255)
                                              cGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCg                                                >  2:2896393/1‑100 (MQ=255)
                                               gCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGCACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCgg                                               <  1:1783859/100‑1 (MQ=255)
                                               gCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCgg                                               <  1:967020/100‑1 (MQ=255)
                                                cAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGa                                              <  2:1467112/100‑1 (MQ=255)
                                                   gTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCa                                           >  1:1089760/1‑100 (MQ=255)
                                                    tGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTTGGCAGCGGGATCGGACCAc                                          <  1:773502/100‑1 (MQ=255)
                                                      aGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACgg                                        <  1:1472525/100‑1 (MQ=255)
                                                       gTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGa                                       >  2:460507/1‑100 (MQ=255)
                                                         aCGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGAcc                                     <  2:4145/100‑1 (MQ=255)
                                                         aCGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGAcc                                     <  2:4396/100‑1 (MQ=255)
                                                          cGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCt                                    >  2:1257763/1‑100 (MQ=255)
                                                            gATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTgc                                  <  2:208844/100‑1 (MQ=255)
                                                             aTTTCGTACTGATCTCTCGCGTCTTTGCTGACGTACGCGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGACAGCGTTATCGGACCACGGACCTacg                                 <  1:838985/100‑1 (MQ=255)
                                                                 cGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCAcc                             >  1:337763/1‑100 (MQ=255)
                                                                 cGTACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCAcc                             <  2:360999/100‑1 (MQ=255)
                                                                   tACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAg                           >  2:1866370/1‑100 (MQ=255)
                                                                   tACTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAg                           <  1:2330653/100‑1 (MQ=255)
                                                                     cTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTg                         >  1:482205/1‑100 (MQ=255)
                                                                     cTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTg                         <  1:652101/100‑1 (MQ=255)
                                                                     cTGATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTg                         >  1:2299674/1‑100 (MQ=255)
                                                                       gATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTgcg                       >  2:316503/1‑100 (MQ=255)
                                                                       gATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTgcg                       >  2:316584/1‑100 (MQ=255)
                                                                       gATCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTgcg                       >  1:2858196/1‑100 (MQ=255)
                                                                        aTCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACTGGTgcgc                      >  2:1058617/1‑100 (MQ=255)
                                                                        aTCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTgcgc                      >  2:2504715/1‑100 (MQ=255)
                                                                         tCGCGCGCGTCTTTGTTGACGTACGGGTAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCt                     <  2:981086/100‑1 (MQ=255)
                                                                         tCGCGCGCGTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACTGGTgcgca                     <  1:2882946/100‑2 (MQ=255)
                                                                           tccgatctCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACTGGTgcgcagg                   <  2:72474/93‑4 (MQ=255)
                                                                           gcgcgcgTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACTGGTgcgcagg                   <  1:1058603/100‑4 (MQ=255)
                                                                           gcgcgcgTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACTGGTgcgcagg                   <  2:2831530/100‑4 (MQ=255)
                                                                           gcgcgcgTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTg                   <  1:55661/100‑1 (MQ=255)
                                                                             gcgcgTCTTTGTTGATGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGc                 <  2:807471/100‑1 (MQ=255)
                                                                             gcgcgTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGc                 >  2:2153842/1‑100 (MQ=255)
                                                                             gcgcgTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGc                 <  1:1517042/100‑1 (MQ=255)
                                                                             gcgcgTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGc                 <  2:953556/100‑1 (MQ=255)
                                                                             gcgcgTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGc                 <  2:2220150/100‑1 (MQ=255)
                                                                              cgcgTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCa                >  2:1211666/1‑100 (MQ=255)
                                                                               gcgTCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCAGCGCACTGGTgcgcaggtccg               <  1:2305663/100‑8 (MQ=255)
                                                                                  tCTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGtct            <  2:411019/100‑1 (MQ=255)
                                                                                   cTTTGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGtctc           >  1:2137870/1‑100 (MQ=255)
                                                                                    tttGTTGACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGGCTcg          <  1:300272/100‑1 (MQ=255)
                                                                                      tgttgACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTcgac        <  1:2886458/100‑1 (MQ=255)
                                                                                      tgttgACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTcgac        <  1:2004749/100‑1 (MQ=255)
                                                                                      tgttgACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTcgac        <  1:1752770/100‑1 (MQ=255)
                                                                                      tgttgACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGCATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTcgac        >  1:1001181/1‑100 (MQ=255)
                                                                                        ttgACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTcgacga      >  2:236472/1‑100 (MQ=255)
                                                                                         tgACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGAt     <  2:1513612/100‑1 (MQ=255)
                                                                                         tgACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGAt     <  2:2494920/100‑1 (MQ=255)
                                                                                          gACGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGAtt    >  1:1701197/1‑100 (MQ=255)
                                                                                            cGTACGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTc  >  1:897824/1‑100 (MQ=255)
                                                                                               |                                                                                                
CAGAGCATCAACGGTTTTCTCGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTC  >  NC_000913/3453010‑3453201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A