Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F232 I1 R1 72 114.8 5816246 94.5% 5496352 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,966,720 C→T R102C (CGC→TGC)  rho → transcription termination factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,966,7200CT97.3% 367.1 / ‑2.5 110R102C (CGC→TGC) rhotranscription termination factor
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (59/48);  total (60/50)
Fisher's exact test for biased strand distribution p-value = 5.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

CAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTG  >  NC_000913/3966623‑3966816
                                                                                                 |                                                                                                
 aGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTtgcc                                                                                               <  1:2070907/100‑1 (MQ=255)
 aGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTtgcc                                                                                               <  2:92578/100‑1 (MQ=255)
  gCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTtgccc                                                                                              >  2:1108138/1‑100 (MQ=255)
  gCTCCTACCTCGCCGGTCCTGATGACATCTACCTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTtgc                                                                                                <  1:860722/98‑1 (MQ=255)
    tCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTtgcccgc                                                                                            >  1:496306/1‑100 (MQ=255)
     ccTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTtgcccgcc                                                                                           >  2:2814159/1‑100 (MQ=255)
     ccTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTtgcccgcc                                                                                           >  1:2170799/1‑100 (MQ=255)
      cTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCccgccg                                                                                          <  1:2401352/100‑1 (MQ=255)
            cGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAaagaa                                                                                    >  2:1279251/1‑100 (MQ=255)
             gCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAaagaag                                                                                   >  1:378254/1‑100 (MQ=255)
             gCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAaagaag                                                                                   >  2:746174/1‑100 (MQ=255)
             gCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAaagaag                                                                                   >  2:2635988/1‑100 (MQ=255)
              ccGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAgg                                                                                  <  2:1407779/100‑1 (MQ=255)
              ccGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAgg                                                                                  >  1:20236/1‑100 (MQ=255)
               cGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGt                                                                                 >  2:2610475/1‑100 (MQ=255)
                ggTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTg                                                                                <  1:1152676/100‑1 (MQ=255)
                     tgatgaCATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGc                                                                           <  2:1561062/100‑1 (MQ=255)
                     tgatgaCATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGc                                                                           >  1:2559734/1‑100 (MQ=255)
                     tgatgaCATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGc                                                                           >  2:345514/1‑100 (MQ=255)
                      gatgaCATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCt                                                                          <  1:1471127/100‑1 (MQ=255)
                      gatgaCATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCt                                                                          >  2:657047/1‑100 (MQ=255)
                      gatgaCATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCt                                                                          >  1:2020610/1‑100 (MQ=255)
                        tgaCATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTAt                                                                        >  2:1654744/1‑100 (MQ=255)
                           cATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTAtttt                                                                     >  2:1673309/1‑100 (MQ=255)
                           cATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTAtttt                                                                     >  2:2185163/1‑100 (MQ=255)
                                aCGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCgct                                                                <  1:749959/100‑1 (MQ=255)
                                  gtgtCCCATAGCCAACTCCGCCGGTTCAACCTCCCCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCTAAAGAAGGTGAACGCTATTTTGCgctgc                                                              <  1:614447/97‑1 (MQ=255)
                                  gTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCgctgc                                                              >  2:2442096/1‑100 (MQ=255)
                                   tttCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCgctgct                                                             <  1:1710641/100‑1 (MQ=255)
                                   tttCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCgctgct                                                             >  2:139416/1‑100 (MQ=255)
                                       cccTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGaaa                                                         >  1:2509547/1‑100 (MQ=255)
                                        ccTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAg                                                        <  2:1292613/100‑1 (MQ=255)
                                        ccTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAg                                                        >  2:2121377/1‑100 (MQ=255)
                                          tAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGtt                                                      <  1:1685559/100‑1 (MQ=255)
                                           aGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTa                                                     >  1:1600930/1‑100 (MQ=255)
                                            gCCAACTCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTaa                                                    <  1:1923921/100‑1 (MQ=255)
                                            gCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTaa                                                    >  2:1574755/1‑100 (MQ=255)
                                            gCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCCGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTaa                                                    >  2:169488/1‑100 (MQ=255)
                                             ccAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAAc                                                   <  1:968349/100‑1 (MQ=255)
                                              cAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACg                                                  >  1:950887/1‑100 (MQ=255)
                                               aaaTCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTATGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGa                                                 <  2:2383100/100‑1 (MQ=255)
                                               aaaTCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGa                                                 <  2:1989022/100‑1 (MQ=255)
                                                 aTCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAg                                               >  2:1881848/1‑100 (MQ=255)
                                                 aTCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAg                                               >  1:129319/1‑100 (MQ=255)
                                                 aTCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAAGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAg                                               >  2:2037266/1‑100 (MQ=255)
                                                  tCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGt                                              <  2:2805633/100‑1 (MQ=255)
                                                   ccgccgTTTCAACCTCCTCACTGGTGATACCATCTCTAGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGa                                                 <  1:2814123/96‑1 (MQ=255)
                                                   ccgccgTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAggt                                             <  2:1951752/100‑3 (MQ=255)
                                                    cgccgTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTa                                            <  2:2029695/100‑1 (MQ=255)
                                                    cgccgTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAActtcgtt                                             <  1:2281567/99‑7 (MQ=255)
                                                    cgccgTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTa                                            <  1:1890099/100‑1 (MQ=255)
                                                    cgccgTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTa                                            >  1:1065102/1‑100 (MQ=255)
                                                        gTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACtt                                        >  2:2318901/1‑100 (MQ=255)
                                                        gTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACtt                                        >  2:2566030/1‑100 (MQ=255)
                                                         tttCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTc                                       <  1:1279233/100‑1 (MQ=255)
                                                          ttCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCg                                      <  2:2872100/100‑1 (MQ=255)
                                                          ttCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCg                                      <  1:104654/100‑1 (MQ=255)
                                                           tCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGa                                     >  1:459426/1‑100 (MQ=255)
                                                           tCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGa                                     <  1:1491669/100‑1 (MQ=255)
                                                           tCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGa                                     >  2:950370/1‑100 (MQ=255)
                                                            cAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCTAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGAc                                    <  2:2303783/100‑1 (MQ=255)
                                                            cAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGAc                                    >  2:2471672/1‑100 (MQ=255)
                                                             aaCCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACa                                   <  1:86664/100‑1 (MQ=255)
                                                             aaCCTCCGCACTGGGGATACCATCTCTTGTAAGTTTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGAc                                    <  1:910248/99‑1 (MQ=255)
                                                               ccTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACtttcagcag                                 <  2:812493/100‑8 (MQ=255)
                                                               ccTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACaaa                                 >  1:17130/1‑100 (MQ=255)
                                                                cTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAAc                                <  2:1114106/100‑1 (MQ=255)
                                                                 tCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAAcc                               <  1:139413/100‑1 (MQ=255)
                                                                  ccGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCt                              >  2:1555483/1‑100 (MQ=255)
                                                                   cGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTg                             <  2:2509053/100‑1 (MQ=255)
                                                                   cGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTg                             <  2:897317/100‑1 (MQ=255)
                                                                        tGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGaaaa                         <  2:2496771/99‑1 (MQ=255)
                                                                         ggTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACg                       >  2:2133884/1‑100 (MQ=255)
                                                                         ggTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACg                       >  1:2327627/1‑100 (MQ=255)
                                                                         ggTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACg                       >  2:1603112/1‑100 (MQ=255)
                                                                          gTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGc                      >  1:967174/1‑100 (MQ=255)
                                                                           tGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGcc                     >  1:2185453/1‑100 (MQ=255)
                                                                           tGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGcc                     >  2:298869/1‑100 (MQ=255)
                                                                           tGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGc                      >  1:2214359/1‑99 (MQ=255)
                                                                            gATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGccc                    <  2:498770/100‑1 (MQ=255)
                                                                            gATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGccc                    >  2:2130326/1‑100 (MQ=255)
                                                                             aTACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGccn                    <  1:2185136/99‑2 (MQ=255)
                                                                             aTACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCg                   >  1:1060071/1‑100 (MQ=255)
                                                                             aTACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCg                   <  2:2246254/100‑1 (MQ=255)
                                                                             aTACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCg                   <  2:2436560/100‑1 (MQ=255)
                                                                              tACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGc                  <  2:2333805/100‑1 (MQ=255)
                                                                              tACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGc                  >  1:524550/1‑100 (MQ=255)
                                                                                 cATCTCTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcaac               <  1:2610442/100‑1 (MQ=255)
                                                                                   tctcTGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcaacaa             >  1:2183820/1‑100 (MQ=255)
                                                                                     tctGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACaaaa           >  2:420554/1‑100 (MQ=255)
                                                                                     tctGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACaaaa           <  2:2077739/100‑1 (MQ=255)
                                                                                      ctGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAAt          <  2:2163677/100‑1 (MQ=255)
                                                                                      ctGGTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAAt          <  1:1108120/100‑1 (MQ=255)
                                                                                        ggTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATcc        >  2:1392145/1‑100 (MQ=255)
                                                                                        ggTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATcc        >  1:802599/1‑100 (MQ=255)
                                                                                         gTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCct       >  2:590402/1‑100 (MQ=255)
                                                                                         gTAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCct       >  1:183213/1‑100 (MQ=255)
                                                                                          tAAGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCGGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCctc      <  1:2358986/100‑1 (MQ=255)
                                                                                           aaGATTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCctct     <  2:2020638/100‑1 (MQ=255)
                                                                                              aTTTGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTg  >  2:1561274/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTG  >  NC_000913/3966623‑3966816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A