Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F232 I1 R1 72 114.8 5816246 94.5% 5496352 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,185,211 T→C F1323L (TTC→CTC)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,185,2110TC98.0% 513.2 / ‑2.1 148F1323L (TTC→CTC) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (74/70);  total (76/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 148 total aligned reads displayed.

CTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGTCGGGTTAAAA  >  NC_000913/4185115‑4185309
                                                                                                |                                                                                                  
cTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCCAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCa                                                                                                 <  1:1480940/100‑1 (MQ=255)
cTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCa                                                                                                 >  1:982169/1‑100 (MQ=255)
  cACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAAc                                                                                               >  1:1595927/1‑100 (MQ=255)
    ccGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGt                                                                                             >  2:1535035/1‑100 (MQ=255)
        tAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTAttg                                                                                         >  2:2545062/1‑100 (MQ=255)
         ccgatctatgaCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTAttgt                                                                                        <  2:2885839/93‑1 (MQ=255)
         aaGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTAttgt                                                                                        <  2:1654745/100‑1 (MQ=255)
         aaGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTAttgt                                                                                        <  1:121993/100‑1 (MQ=255)
         aaGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTAttgt                                                                                        >  1:2396313/1‑100 (MQ=255)
         aaGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCACTCAACGTAt                                                                                           >  1:735017/1‑97 (MQ=255)
           gTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTAttgttg                                                                                      <  1:419476/100‑1 (MQ=255)
           gTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTAttgttg                                                                                      <  1:2603525/100‑1 (MQ=255)
           gTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTAttgttg                                                                                      >  2:2290408/1‑100 (MQ=255)
           gTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTAttgttg                                                                                      <  2:242770/100‑1 (MQ=255)
            tCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTAttgttgn                                                                                     <  2:158131/100‑2 (MQ=255)
            tCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGa                                                                                     >  2:322071/1‑100 (MQ=255)
            tCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGa                                                                                     >  2:2620677/1‑100 (MQ=255)
             cTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGaa                                                                                    <  1:2520453/100‑1 (MQ=255)
              tgatgaCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGaaa                                                                                   >  2:856860/1‑100 (MQ=255)
              tgatgaCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGaaa                                                                                   <  1:2197751/100‑1 (MQ=255)
              tgatgaCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGaaa                                                                                   >  1:1403874/1‑100 (MQ=255)
               gatgaCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAag                                                                                  >  2:1428436/1‑100 (MQ=255)
               gatgaCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAag                                                                                  <  2:1098404/100‑1 (MQ=255)
               gatgaCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAag                                                                                  <  1:1706630/100‑1 (MQ=255)
               gatgaCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAag                                                                                  >  1:576480/1‑100 (MQ=255)
               gatgaCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAag                                                                                  <  1:78258/100‑1 (MQ=255)
                atgaCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTAttgtagatcgg                                                                                 >  1:2885801/1‑93 (MQ=255)
                atgaCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAaga                                                                                 <  1:2443211/100‑1 (MQ=255)
                atgaCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAaga                                                                                 <  1:2485110/100‑1 (MQ=255)
                 tgaCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAagag                                                                                >  2:1989683/1‑100 (MQ=255)
                  gaCGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAgaga                                                                               <  1:56981/100‑1 (MQ=255)
                   aggTGAACGGTCGTACCAAGCTGTATAAACACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCATCAACGTATTGTTGAAAGAGAt                                                                              <  2:575622/98‑1 (MQ=255)
                    cGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGAtt                                                                             >  1:613828/1‑100 (MQ=255)
                    cGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGAtt                                                                             >  1:2361963/1‑100 (MQ=255)
                     gTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGAttc                                                                            >  1:1836526/1‑100 (MQ=255)
                       gAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGAttcgt                                                                          <  1:1370222/100‑1 (MQ=255)
                       gAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGAttcgt                                                                          >  2:2065264/1‑100 (MQ=255)
                        aaCGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGAttcgtt                                                                         <  1:2315641/100‑1 (MQ=255)
                         aCGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGAttcgttc                                                                        >  1:213664/1‑100 (MQ=255)
                         aCGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGAttcgttc                                                                        <  2:1601892/100‑1 (MQ=255)
                         aCGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGAttcgttc                                                                        <  2:2298561/100‑1 (MQ=255)
                            gTCGTACCAAGATGTATAAAAGCATCGTGGACGGCAACCATCAGATGGAGCAGGGCATGCCAGAATCCCTCAACGTATTGGTGAAAGAGAttcgttcg                                                                       <  1:1978942/98‑1 (MQ=255)
                            gTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCt                                                                     >  2:942508/1‑100 (MQ=255)
                             tCGTACCAAGATGTATAAAAACATCGTGGACGGCTACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTg                                                                    <  1:1535011/100‑1 (MQ=255)
                             tCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGATGCCCACAACGTATTGTTGAAAGAGATTCGTTCGCTg                                                                    <  2:213667/100‑1 (MQ=255)
                             tCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTg                                                                    <  1:908087/100‑1 (MQ=255)
                              cGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTgg                                                                   >  1:1928697/1‑100 (MQ=255)
                              cGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTgg                                                                   >  1:1334688/1‑100 (MQ=255)
                               gTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTggg                                                                  >  1:1680560/1‑100 (MQ=255)
                                tACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGt                                                                 >  1:2160103/1‑100 (MQ=255)
                                 aCCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTa                                                                <  2:1520248/100‑1 (MQ=255)
                                  ccAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTAt                                                               >  2:1580840/1‑100 (MQ=255)
                                       aTGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACa                                                          >  1:2725189/1‑100 (MQ=255)
                                         gTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATc                                                        <  2:2044763/100‑1 (MQ=255)
                                          tataAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCg                                                       <  1:985923/100‑1 (MQ=255)
                                           ataAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGa                                                      >  2:1658463/1‑100 (MQ=255)
                                           ataAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGa                                                      >  2:791211/1‑100 (MQ=255)
                                           ataAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAAGCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCg                                                       <  1:1241191/99‑1 (MQ=255)
                                            taAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGaa                                                     >  2:531857/1‑100 (MQ=255)
                                            taAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGaa                                                     >  1:854043/1‑100 (MQ=255)
                                             aaaaaCATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAAc                                                    >  2:710966/1‑100 (MQ=255)
                                             aaaaaCATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAAc                                                    >  1:2704965/1‑100 (MQ=255)
                                              aaaaCATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACt                                                   >  2:2580828/1‑100 (MQ=255)
                                              aaaaCATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACt                                                   >  2:1777757/1‑100 (MQ=255)
                                               aaaCATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTg                                                  >  1:1494942/1‑100 (MQ=255)
                                                 aCATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGa                                                <  1:2290381/100‑1 (MQ=255)
                                                  cATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGaa                                               <  1:290553/100‑1 (MQ=255)
                                                   aTCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAg                                              >  2:123066/1‑100 (MQ=255)
                                                     cGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGAc                                            <  2:709646/100‑1 (MQ=255)
                                                      gTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACg                                           <  1:1218575/100‑1 (MQ=255)
                                                      gTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACg                                           >  2:2254426/1‑100 (MQ=255)
                                                        ggACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAg                                         >  1:1339296/1‑100 (MQ=255)
                                                           cGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTaa                                      >  1:221414/1‑100 (MQ=255)
                                                           cGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTaa                                      >  2:1289164/1‑100 (MQ=255)
                                                                aCCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATtctc                                 <  2:221417/100‑1 (MQ=255)
                                                                 ccATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCg                                >  2:1556005/1‑100 (MQ=255)
                                                                  cATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGc                               <  1:1073285/100‑1 (MQ=255)
                                                                   aTCAGATGGAGCCGGGCATGCCAGGATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATCCTCGCt                              <  2:445176/100‑1 (MQ=255)
                                                                    tCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTc                             <  2:576486/100‑1 (MQ=255)
                                                                      aGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCGGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCaa                           >  2:222170/1‑100 (MQ=255)
                                                                             gCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTc                    >  2:1204572/1‑100 (MQ=255)
                                                                                  gCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCActgc               <  2:1514470/100‑1 (MQ=255)
                                                                                  gCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCActgc               >  1:181874/1‑100 (MQ=255)
                                                                                     tGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGt            >  1:1625175/1‑100 (MQ=255)
                                                                                      gCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGTc           >  2:2337756/1‑100 (MQ=255)
                                                                                      gCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGTc           >  2:2337794/1‑100 (MQ=255)
                                                                                       ccAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGACGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGTCg          <  1:1555980/100‑1 (MQ=255)
                                                                                       ccAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGTCg          <  2:2238693/100‑1 (MQ=255)
                                                                                       ccAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGTCg          <  1:2567714/100‑1 (MQ=255)
                                                                                       ccAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGTCg          <  1:2568259/100‑1 (MQ=255)
                                                                                       ccAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGTCg          >  2:137131/1‑100 (MQ=255)
                                                                                        cAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTTTCGCTCAAACAGGTCACTGCTGTCgg         <  2:1768772/100‑1 (MQ=255)
                                                                                         aGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTTACTGCTGTCggg        <  2:1909166/100‑1 (MQ=255)
                                                                                         aGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGTCggg        <  2:1625200/100‑1 (MQ=255)
                                                                                          gAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGTCGGGt       >  1:2435234/1‑100 (MQ=255)
                                                                                           aaTCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGTCGGGtt      <  1:2620644/100‑1 (MQ=255)
                                                                                            aTCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGTCGGGTTa     <  1:942499/100‑1 (MQ=255)
                                                                                            aTCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGTCGGGTTa     <  1:228442/100‑1 (MQ=255)
                                                                                             tCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGTCGGGTTaa    >  2:945102/1‑100 (MQ=255)
                                                                                               cTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGTCGGGTTaaaa  >  1:1317343/1‑100 (MQ=255)
                                                                                                |                                                                                                  
CTCACCGTTAAGTCTGATGACGTGAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGTCGGGTTAAAA  >  NC_000913/4185115‑4185309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A