Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A16 F258 I1 R1	132	116.1	5785507	94.2%	5449947	99.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,070,123	(A)_7→6	intergenic (‑316/+42)	mscS ← / ← fbaA	mechanosensitive channel protein, small conductance/fructose‑bisphosphate aldolase, class II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,070,117	0	A	.	99.2%	519.7 / 0.5	122	intergenic (‑310/+48)	mscS/fbaA	mechanosensitive channel protein, small conductance/fructose‑bisphosphate aldolase, class II
Reads supporting (aligned to +/- strand): ref base A (1/0); new base . (60/59); total (62/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 130 total aligned reads displayed.

AACGCCTTATCCGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCGAAAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGTTCAGTTCCTGGAATGCTTTCTCCAGACG  >  NC_000913/3070018‑3070212

 aCGCCTTATCCGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCGaa                                                                                                 >  2:1980569/1‑100 (MQ=255)

 aCGCCTTATCCGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCGaa                                                                                                 >  1:1812045/1‑100 (MQ=255)

 aCGCCTTATCCGGCCTACAAAAACCTGCAAATTCAATATATAGCAGGATACGTGTAGGCCTAATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCGaa                                                                                                 >  2:1580009/1‑100 (MQ=255)

     cTTATCCGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCTTAGCGCATCAGGCAATTTTGCTTTTGGCGaaaaaa                                                                                             <  2:1212544/100‑1 (MQ=255)

     cTTATCCGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCGaaaaaa                                                                                             <  1:2394368/100‑1 (MQ=255)

         tCCGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCGaaaaaagacc                                                                                         >  2:2194166/1‑96 (MQ=255)

           cGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTTGCG‑AAAAAAGAccc                                                                                       >  2:2351475/1‑99 (MQ=255)

           cGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGAccc                                                                                       >  2:2351436/1‑99 (MQ=255)

           cGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCg                                                                                      <  1:2500928/100‑1 (MQ=255)

           cGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCg                                                                                      <  1:1473998/100‑1 (MQ=255)

            ggCCTACAAAAACCTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGc                                                                                     >  2:849405/1‑100 (MQ=255)

            ggCCTACAAAAACCTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGc                                                                                     <  2:1907812/100‑1 (MQ=255)

            ggCCTACAAAAACCTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGc                                                                                     >  1:323888/1‑100 (MQ=255)

            ggCCTACAAAAACCTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGc                                                                                     <  1:2566087/100‑1 (MQ=255)

            cgatctcAAAAACCTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGc                                                                                     <  1:1025326/94‑1 (MQ=21)

                tACAAAAACCTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCagag                                                                                 >  2:2831738/1‑100 (MQ=255)

                  cAAAAACCTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCagatc                                                                                >  2:1025324/1‑97 (MQ=255)

                  cAAAAACCTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCg                                                                               >  1:2670809/1‑100 (MQ=255)

                  cAAAAACCTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTAAAAAAAGAG‑AAAAAAGACCCGCAGAGCg                                                                               <  2:2250192/100‑1 (MQ=17)

                    caaaCCTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCGAAAAAAAGACCCGCAGAGCg                                                                               >  2:2806369/2‑99 (MQ=255)

                    aaaaCCTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AACAAAGACCCGCAGAGCggg                                                                             >  1:770766/1‑100 (MQ=255)

                    aaaaCCTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCg                                                                               >  2:2401215/1‑98 (MQ=255)

                     aaaCCTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGc                                                                            <  1:851516/100‑1 (MQ=255)

                        ccTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCtt                                                                         >  2:1779992/1‑100 (MQ=255)

                        ccTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCtt                                                                         >  2:1173631/1‑100 (MQ=255)

                         cTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTg                                                                        <  2:2438901/100‑1 (MQ=255)

                         cTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTg                                                                        >  2:1088715/1‑100 (MQ=255)

                          tGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTga                                                                       >  2:2846848/1‑100 (MQ=255)

                           gCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTgag                                                                      <  1:116292/100‑1 (MQ=255)

                           gCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTgag                                                                      >  1:2392138/1‑100 (MQ=255)

                            cAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTgaga                                                                     >  1:2837153/1‑100 (MQ=255)

                             aaaTTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGGTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGAt                                                                    >  2:749300/1‑100 (MQ=255)

                              aaTTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATa                                                                   <  1:557210/100‑1 (MQ=255)

                              aaTTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATa                                                                   >  1:1984364/1‑100 (MQ=255)

                                ttCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAg                                                                 <  2:1224245/100‑1 (MQ=255)

                                ttCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAg                                                                 >  2:556783/1‑100 (MQ=255)

                                ttCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAg                                                                 <  1:2071637/100‑1 (MQ=255)

                                 tCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGc                                                                <  2:800614/100‑1 (MQ=255)

                                  cAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCCTCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGAt                                                                    >  2:1440326/1‑95 (MQ=255)

                                  cAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCa                                                               <  2:2398076/100‑1 (MQ=255)

                                  cAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCa                                                               <  2:2263038/100‑1 (MQ=255)

                                       tatTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAg                                                          <  1:134792/100‑1 (MQ=255)

                                       tatTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAg                                                          <  2:1851142/100‑1 (MQ=255)

                                       tatTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAg                                                          <  2:225257/100‑1 (MQ=255)

                                       tatTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAg                                                          <  2:2071290/100‑1 (MQ=255)

                                       tatTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAg                                                          >  1:1099730/1‑100 (MQ=255)

                                       tatTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAg                                                          <  1:1422026/100‑1 (MQ=255)

                                        atTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAgg                                                         <  1:1756118/100‑1 (MQ=255)

                                          tGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGaa                                                       <  2:2579283/100‑1 (MQ=255)

                                          tGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGaa                                                       >  1:2401562/1‑100 (MQ=255)

                                           gCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAGGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAat                                                      <  1:1143173/100‑1 (MQ=255)

                                           gCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAat                                                      >  2:1618771/1‑100 (MQ=255)

                                              ggATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATc                                                   >  1:624105/1‑100 (MQ=255)

                                              ggATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATc                                                   >  1:1221977/1‑100 (MQ=255)

                                              ggATACGTGTAGGCCTGATAAGCGTAGCGCATAAGGCAATTTTGCTTTTGGCG‑AAAAAAGAACCGCAGAGCGGGCATTGAGATAAGCAGAAAGGAatat                                                    >  1:63712/1‑99 (MQ=37)

                                              ggATACGTGTAGGCCTGATAAGCGTACCGCACCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATc                                                   <  1:1394163/100‑1 (MQ=38)

                                                  ttccgatctccTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTAc                                               <  1:2243470/91‑1 (MQ=37)

                                                  aCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTAc                                               <  2:2784123/100‑1 (MQ=255)

                                                  aCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTAc                                               <  2:823946/100‑1 (MQ=255)

                                                     tgtAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGTTTTT‑TTTTTAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGa                                            >  2:1415798/1‑100 (MQ=25)

                                                     tgtAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGa                                            >  1:473883/1‑100 (MQ=255)

                                                      gtAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGaa                                           >  1:1308802/1‑100 (MQ=255)

                                                         ggCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGt                                        >  2:2522098/1‑100 (MQ=255)

                                                         ggCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGt                                        >  2:82377/1‑100 (MQ=255)

                                                          gCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTc                                       <  1:880929/100‑1 (MQ=255)

                                                           ccTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCg                                      >  2:315966/1‑100 (MQ=255)

                                                            cTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGa                                     <  1:2276426/100‑1 (MQ=255)

                                                              gATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATc                                   <  2:1984350/100‑1 (MQ=255)

                                                               aTAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATcg                                  <  1:1455856/100‑1 (MQ=255)

                                                                  aGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGt                              <  2:1812032/100‑1 (MQ=255)

                                                                  aGCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGt                              <  2:1052494/100‑1 (MQ=255)

                                                                   gCGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGtt                             >  2:1123338/1‑100 (MQ=255)

                                                                    cGTAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGTTc                            <  2:1221973/100‑1 (MQ=255)

                                                                      tAGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGTTCAg                          <  2:505184/100‑1 (MQ=255)

                                                                       aGCGCATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGTTCAGt                         >  1:1815218/1‑100 (MQ=255)

                                                                         cgcATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGTTCAGTTc                       <  1:1394239/100‑1 (MQ=255)

                                                                          gcATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGTTCAGTTcc                      >  2:418005/1‑100 (MQ=255)

                                                                          gcATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGTTCAGTTcc                      >  1:1507408/1‑100 (MQ=255)

                                                                          gcATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGTTCAGTTcc                      >  2:2437629/1‑100 (MQ=255)

                                                                           cATCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACTGAACG‑CGATCGACGTTctgtaaga                     <  1:142199/100‑8 (MQ=37)

                                                                            aTCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGTTCAGTTCCTg                    <  2:323887/100‑1 (MQ=255)

                                                                            aTCAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGTTCAGTTCCTg                    <  1:380312/100‑1 (MQ=255)

                                                                              cAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGTTCAGTTCCTGGa                  >  1:100321/1‑100 (MQ=255)

                                                                              cAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGTTCAGTTCCTGGa                  <  2:426502/100‑1 (MQ=255)

                                                                              cAGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGTTCAGTTCCTGGa                  >  1:585990/1‑100 (MQ=255)

                                                                               agatcaTTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACG‑CGATCGACGTTCTGtaa                       <  2:2175815/89‑3 (MQ=25)

                                                                               aGGCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGTTCAGTTCCTGGaa                 >  2:880655/1‑100 (MQ=255)

                                                                                ggCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGTTCAGTTCCTGGAAt                >  2:431113/1‑100 (MQ=255)

                                                                                ggCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGTTCAGTTCCTGGAAt                >  2:929541/1‑100 (MQ=255)

                                                                                ggCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGTTCAGTTCCTGGAAt                >  1:287139/1‑100 (MQ=255)

                                                                                ggCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGTTCAGTTCCTGGAAt                >  2:2510274/1‑100 (MQ=255)

                                                                                ggCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGTTCAGTTCCTGGAAt                >  1:942908/1‑100 (MQ=255)

                                                                                ggCAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGTTCAGTTCCTGGAAt                >  2:2156560/1‑100 (MQ=255)

                                                                                  cAATTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGTTCAGTTCCTGGAATGc              <  2:1157656/100‑1 (MQ=255)

                                                                                    aTTTTGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGTTCAGTTCCTGGAATGCtt            >  2:691590/1‑100 (MQ=255)

                                                                                      tttGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGTTCAGTTCCTGGAATGCTTtc          <  1:699216/100‑1 (MQ=255)

                                                                                      tttGCTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATATTACAGAACGTCGATCG‑CGTTCAGTTCCTGGAATGCTTtc          <  2:2474951/100‑1 (MQ=255)

                                                                                          cTTTTGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGTTCAGTTCCTGGAATGCTTTCTCCa      >  1:1372078/1‑100 (MQ=255)

                                                                                           ttttGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGTTCAGTTCCTGGAATGCTTTCTCCAg     <  1:1088718/100‑1 (MQ=255)

                                                                                              tGGCG‑AAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGTTCAGTTCCTGGAATGCTTTCTCCAGACg  >  2:374902/1‑100 (MQ=255)

AACGCCTTATCCGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGATACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGGCGAAAAAAAGACCCGCAGAGCGGGCCTTGAGATAAGCAGAAAGGAATATCTTACAGAACGTCGATCG‑CGTTCAGTTCCTGGAATGCTTTCTCCAGACG  >  NC_000913/3070018‑3070212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A