Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F258 I1 R1 132 116.1 5785507 94.2% 5449947 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,128,259 A→G V46A (GTG→GCG)  metJ ← transcriptional repressor, S‑adenosylmethionine‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,128,2590AG97.5% 412.3 / ‑0.8 122V46A (GTG→GCG) metJtranscriptional repressor, S‑adenosylmethionine‑binding
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (65/53);  total (67/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 122 total aligned reads displayed.

ACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTG  >  NC_000913/4128162‑4128353
                                                                                                 |                                                                                              
aCGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGcc                                                                                              <  2:344403/100‑1 (MQ=255)
aCGCAGATCGCCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTCGTAGCGTGACGCAGGTTGTTCGcc                                                                                              <  2:386926/100‑1 (MQ=255)
 cGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCt                                                                                             >  1:2825405/1‑100 (MQ=255)
 cGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCt                                                                                             >  1:762956/1‑100 (MQ=255)
 cGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCt                                                                                             >  2:186394/1‑100 (MQ=255)
  gCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTg                                                                                            <  2:898401/100‑1 (MQ=255)
  gCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTg                                                                                            <  1:2066582/100‑1 (MQ=255)
     gATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTgacg                                                                                         >  1:899349/1‑100 (MQ=255)
      aTCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTgacgn                                                                                        <  2:1866679/100‑2 (MQ=255)
       tCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTgacgac                                                                                       >  1:2560037/1‑100 (MQ=255)
         ggCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGAcgc                                                                                     >  2:1127991/1‑100 (MQ=255)
          gCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGAcgcg                                                                                    <  1:2389546/100‑1 (MQ=255)
          gCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGAcgcg                                                                                    >  1:768174/1‑100 (MQ=255)
          gCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGAcgcg                                                                                    <  1:2314608/100‑1 (MQ=255)
          gCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGAcgcg                                                                                    >  1:2800474/1‑100 (MQ=255)
          gCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGAcgcg                                                                                    <  1:1475882/100‑1 (MQ=255)
          gCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGAcgcg                                                                                    >  1:2828088/1‑100 (MQ=255)
            atcatcCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACgcgtg                                                                                  <  2:2222886/100‑1 (MQ=255)
              catcCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACgcgtgcg                                                                                <  1:2086104/100‑1 (MQ=255)
               atcCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACgcgtgcgt                                                                               <  1:74169/100‑1 (MQ=255)
                 ccGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTc                                                                             >  1:707977/1‑100 (MQ=255)
                 ccGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTc                                                                             <  2:2686687/100‑1 (MQ=255)
                 ccGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTc                                                                             <  1:1499245/100‑1 (MQ=255)
                  cGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCa                                                                            >  2:2473698/1‑100 (MQ=255)
                  cGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCa                                                                            >  1:648914/1‑100 (MQ=255)
                   ggCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCAt                                                                           >  2:561663/1‑100 (MQ=255)
                    gCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATc                                                                          <  1:969176/100‑1 (MQ=255)
                    gCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATc                                                                          <  1:2143000/100‑1 (MQ=255)
                    gCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATc                                                                          >  1:1350748/1‑100 (MQ=255)
                      aaaGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCgg                                                                        >  1:501079/1‑100 (MQ=255)
                          gTTGCCCGGTAAAGGTATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAg                                                                    <  1:2843774/100‑1 (MQ=255)
                           ttGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAgg                                                                   >  1:2496467/1‑100 (MQ=255)
                             gCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGAt                                                                 >  1:232121/1‑100 (MQ=255)
                              cccGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGAtt                                                                >  2:2859619/1‑100 (MQ=255)
                              cccGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGAt                                                                 <  2:612171/99‑1 (MQ=255)
                                cagTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGAtttt                                                              >  1:1953847/3‑100 (MQ=255)
                                  ttccgatcttGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTa                                                            <  2:516404/91‑1 (MQ=255)
                                  gTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTa                                                            <  2:952706/100‑1 (MQ=255)
                                  gTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTa                                                            <  2:1163755/100‑1 (MQ=255)
                                  gTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTa                                                            <  1:1621537/100‑1 (MQ=255)
                                   tAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTaa                                                           >  1:16117/1‑100 (MQ=255)
                                    aaaGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAac                                                          >  2:152634/1‑100 (MQ=255)
                                    aaaGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAac                                                          <  1:1037024/100‑1 (MQ=255)
                                    aaaGGCAGGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCTCCTAACGACGCGTGCGTTCATCGGTGAGGATTTTTa                                                            <  2:2399601/98‑1 (MQ=255)
                                     aaGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAaca                                                         >  1:792692/1‑100 (MQ=255)
                                      aGGCATGAAGAAACGCTTTGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAacac                                                        <  1:509516/100‑1 (MQ=255)
                                       ggCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACACGTGCGTTCATCGGTGAGGATTTTTAACAcc                                                       >  2:327941/1‑100 (MQ=255)
                                       ggCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTAGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACAcc                                                       >  1:2035612/1‑100 (MQ=255)
                                       gacATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACAcc                                                       >  1:1951459/3‑100 (MQ=255)
                                         cATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCtc                                                     <  2:1303482/100‑2 (MQ=255)
                                          aTGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTa                                                    >  1:1403640/1‑100 (MQ=255)
                                          aTGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTa                                                    >  2:1967574/1‑100 (MQ=255)
                                            gCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAag                                                  <  1:2581445/100‑1 (MQ=255)
                                             cAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAaga                                                 >  2:2040589/1‑100 (MQ=255)
                                              aGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAagag                                                <  2:2062073/100‑1 (MQ=255)
                                                 aaCGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGaa                                             >  1:2757760/1‑100 (MQ=255)
                                                 aaCGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGaa                                             >  2:1757427/1‑100 (MQ=255)
                                                  aCGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAAt                                            <  2:1248955/100‑1 (MQ=255)
                                                   cGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATg                                           <  1:152634/100‑1 (MQ=255)
                                                    gCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATg                                           >  2:1184557/1‑99 (MQ=255)
                                                     cTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGa                                         <  1:464258/100‑1 (MQ=255)
                                                     cTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGa                                         >  2:1217444/1‑100 (MQ=255)
                                                        cGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAAc                                      <  2:648912/100‑1 (MQ=255)
                                                         gCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAAcc                                     <  2:16117/100‑1 (MQ=255)
                                                            cagcagCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTa                                  >  2:291310/1‑100 (MQ=255)
                                                            cagcagCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTa                                  >  2:896671/1‑100 (MQ=255)
                                                              gcagcTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAAt                                >  2:2396529/1‑100 (MQ=255)
                                                              gcagcTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAAt                                >  1:1179188/1‑100 (MQ=255)
                                                               cagcTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATc                               <  2:2331453/100‑1 (MQ=255)
                                                                 gcTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCtt                             <  1:1625052/100‑1 (MQ=255)
                                                                 gcTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCtt                             >  2:1469472/1‑100 (MQ=255)
                                                                   tCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCATAATCtttt                           <  1:1764517/100‑1 (MQ=255)
                                                                    cGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCttttt                          <  1:1550528/100‑1 (MQ=255)
                                                                     gCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTg                         <  2:863237/100‑1 (MQ=255)
                                                                     gCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTg                         <  2:2560019/100‑1 (MQ=255)
                                                                      cggTTGGTAGCGTGACGCAGGTGGTTCGCCTGACGACTCTGGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTg                         <  2:2785838/97‑1 (MQ=255)
                                                                       tGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGAc                       >  1:1177813/1‑100 (MQ=255)
                                                                        gTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACt                      <  1:561665/100‑1 (MQ=255)
                                                                         ttGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGTGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACtt                     <  2:191379/100‑1 (MQ=255)
                                                                          tGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTg                    >  1:2340012/1‑100 (MQ=255)
                                                                           ggTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGt                   >  1:1165645/1‑100 (MQ=255)
                                                                            gTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGtt                  <  2:2825384/100‑1 (MQ=255)
                                                                              aGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCa                >  2:848966/1‑100 (MQ=255)
                                                                               gCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCAc               >  1:1841036/1‑100 (MQ=255)
                                                                                cGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCAct              >  2:731943/1‑100 (MQ=255)
                                                                                cGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCAct              <  1:422526/100‑1 (MQ=255)
                                                                                  tGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCActct            <  2:2470000/100‑1 (MQ=255)
                                                                                  tGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCActct            >  2:736998/1‑100 (MQ=255)
                                                                                   gACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACtctt           >  1:2599884/1‑100 (MQ=255)
                                                                                    aCGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACtcttc          <  2:2099139/100‑1 (MQ=255)
                                                                                     ngCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACtcttct         >  2:370098/2‑100 (MQ=255)
                                                                                     cGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGATGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACtcttct         <  2:538694/100‑1 (MQ=255)
                                                                                     cGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACtcttct         >  2:2535820/1‑100 (MQ=255)
                                                                                     cGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACtcttct         >  2:511925/1‑100 (MQ=255)
                                                                                     cGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACtcttct         >  1:1599812/1‑100 (MQ=255)
                                                                                     cGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACtcttct         >  2:170388/1‑100 (MQ=255)
                                                                                      gCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTcttctt        >  2:233484/1‑100 (MQ=255)
                                                                                          gttgttCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCg    <  1:2059334/100‑1 (MQ=255)
                                                                                          gttgttCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCg    <  2:2800453/100‑1 (MQ=255)
                                                                                           ttgttCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGt   >  1:2410293/1‑100 (MQ=255)
                                                                                                 |                                                                                              
ACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTG  >  NC_000913/4128162‑4128353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A