Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A16 F258 I1 R1	132	116.1	5785507	94.2%	5449947	99.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,382,091	C→T	W76* (TGG→TGA)	frdA ←	anaerobic fumarate reductase catalytic and NAD/flavoprotein subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,382,091	0	C	T	99.0%	337.1 / ‑4.6	101	W76* (TGG→TGA)	frdA	anaerobic fumarate reductase catalytic and NAD/flavoprotein subunit
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (51/48); total (52/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01

Only 100 of 101 total aligned reads displayed.

GGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAACCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGG  >  NC_000913/4381996‑4382187

gggCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGt                                                                                              >  2:986776/1‑100 (MQ=255)

  gCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCg                                                                                            <  1:1106890/100‑1 (MQ=255)

   cGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGc                                                                                           >  2:185709/1‑100 (MQ=255)

   cGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGc                                                                                           <  2:2239886/100‑1 (MQ=255)

      cGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGAGGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCAc                                                                                        >  1:2267872/1‑100 (MQ=255)

       ggCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCAcc                                                                                       >  1:1033516/1‑100 (MQ=255)

       ggCTCCATGGGCAACCCCACAGTTCCAGTTGGGTCATTACGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCAcc                                                                                       >  2:1786246/1‑100 (MQ=255)

        gCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCAccc                                                                                      <  2:43050/100‑1 (MQ=255)

        gCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCAcc                                                                                       >  2:1775034/1‑99 (MQ=255)

         cTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCg                                                                                     >  2:91841/1‑100 (MQ=255)

          tCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGc                                                                                    <  1:2488042/100‑1 (MQ=255)

           ccATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCt                                                                                   >  1:1594542/1‑100 (MQ=255)

            cATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTa                                                                                  >  1:1487914/1‑100 (MQ=255)

            cATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTa                                                                                  >  2:1255298/1‑100 (MQ=255)

             aTGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTAc                                                                                 >  2:1654686/1‑100 (MQ=255)

             aTGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTAc                                                                                 <  2:866442/100‑1 (MQ=255)

                ggCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGt                                                                              >  2:1996253/1‑100 (MQ=255)

                 gCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTa                                                                             >  2:1460922/1‑100 (MQ=255)

                 gCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTa                                                                             >  1:2051529/1‑100 (MQ=255)

                   aTCCCCACAGTTCGAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATc                                                                           <  1:825797/100‑1 (MQ=255)

                    tCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCg                                                                          >  1:1774908/1‑100 (MQ=255)

                     ccccACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGt                                                                         <  2:539554/100‑1 (MQ=255)

                     ccccACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGt                                                                         <  1:89321/100‑1 (MQ=255)

                      cccACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTg                                                                        >  1:2760404/1‑100 (MQ=255)

                      cccACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTg                                                                        >  1:1238247/1‑100 (MQ=255)

                        cacaGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGan                                                                      <  2:1037873/100‑2 (MQ=255)

                             ttCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGa                                                                 >  1:27284/1‑100 (MQ=255)

                              tCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGat                                                                >  2:1821124/1‑100 (MQ=255)

                              tCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGat                                                                >  2:1152970/1‑100 (MQ=255)

                              tCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGat                                                                >  2:416001/1‑100 (MQ=255)

                               ccAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGata                                                               <  2:1240206/100‑1 (MQ=255)

                                cAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGatat                                                              <  2:121619/100‑1 (MQ=255)

                                cAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGatat                                                              <  2:121450/100‑1 (MQ=255)

                                 aGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATAtt                                                             <  2:2051515/100‑1 (MQ=255)

                                 aGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATAtt                                                             >  1:745769/1‑100 (MQ=255)

                                   tagggTCATCTCGGTTGGGCAGTAGTACACGAAATAATCCACGACCTCCTGCTCACACAATCAGTCGCAACCCGCTACTGTATCGTGAAAGTGATAtt                                                             <  2:867735/96‑1 (MQ=255)

                                    tGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGa                                                          <  1:185710/100‑1 (MQ=255)

                                    tGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGa                                                          >  1:2664181/1‑100 (MQ=255)

                                       gTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGc                                                       >  1:2767011/1‑100 (MQ=255)

                                         cATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTg                                                     >  2:813148/1‑100 (MQ=255)

                                         cATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTg                                                     <  1:1654699/100‑1 (MQ=255)

                                          aTTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGt                                                    <  2:151286/100‑1 (MQ=255)

                                          aTTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGt                                                    >  2:2841550/1‑100 (MQ=255)

                                           tttCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTc                                                   >  2:2402179/1‑100 (MQ=255)

                                           ttgcGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACACTCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTc                                                   >  1:2412665/4‑100 (MQ=255)

                                            ttCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCa                                                  <  2:2604953/100‑1 (MQ=255)

                                             tCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCAt                                                 <  2:1188916/100‑1 (MQ=255)

                                             tCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCAt                                                 <  1:631696/100‑1 (MQ=255)

                                              cGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATg                                                >  2:1566672/1‑100 (MQ=255)

                                               ggTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGa                                               >  2:1340358/1‑100 (MQ=255)

                                                gTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGAt                                              >  2:2742538/1‑100 (MQ=255)

                                                  tGGGCAGTGGTAGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATcc                                            <  1:1775047/100‑1 (MQ=255)

                                                  tGGGCAGCGGTGGACGACATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGACGCTGTCATGATcc                                            <  1:1579621/100‑1 (MQ=255)

                                                     gCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTgc                                         >  2:1192831/1‑100 (MQ=255)

                                                      cAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTgcg                                        >  1:518045/1‑100 (MQ=255)

                                                       aGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTgcgc                                       <  2:1634099/100‑1 (MQ=255)

                                                        gtggtgGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGcgcg                                      <  2:1981003/100‑1 (MQ=255)

                                                        gtggtgGACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGcgcg                                      <  1:2192065/100‑1 (MQ=255)

                                                           gtggACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACa                                   >  2:2869682/1‑100 (MQ=255)

                                                             ggACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAgc                                 <  1:1462550/100‑1 (MQ=255)

                                                             ggACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAgc                                 >  2:648022/1‑100 (MQ=255)

                                                             ggACGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTACCGTGAAAGTGATATTCCAAGCTGTCATGATCCTGCGCGACAgc                                 >  2:2462981/1‑100 (MQ=255)

                                                               aCGAAATAATCCACGACATCCTGCTCACACAATCAGCCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAgcgg                               <  1:1700700/100‑1 (MQ=255)

                                                                cGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAgcggc                              <  1:231054/100‑1 (MQ=255)

                                                                cGAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAgcggc                              >  2:1035426/1‑100 (MQ=255)

                                                                 gAAATAATCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAgcggcg                             <  1:2046078/100‑1 (MQ=255)

                                                                     taatCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGc                         >  2:2886924/1‑100 (MQ=255)

                                                                       atCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGccc                       <  2:2789195/100‑1 (MQ=255)

                                                                        tCCACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGcccc                      <  1:2404390/100‑1 (MQ=255)

                                                                         ccACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGccccc                     <  1:1236153/100‑1 (MQ=255)

                                                                         ccACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGccccc                     <  1:662264/100‑1 (MQ=255)

                                                                         ccACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGccccc                     <  1:21673/100‑1 (MQ=255)

                                                                         ccACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGccccc                     >  2:1294141/1‑100 (MQ=255)

                                                                         ccACGACATCCTGATCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGccccc                     >  2:1789426/1‑100 (MQ=255)

                                                                          cACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCt                    >  1:2767105/1‑100 (MQ=255)

                                                                          cACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCt                    <  1:91841/100‑1 (MQ=255)

                                                                          cACGACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCt                    <  2:2760383/100‑1 (MQ=255)

                                                                             gACATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCt                 <  2:944933/100‑1 (MQ=255)

                                                                              aCATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTg                >  1:1434895/1‑100 (MQ=255)

                                                                               ttccgatcTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTgc               <  1:41415/93‑1 (MQ=255)

                                                                               cATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTgc               >  2:1378938/1‑100 (MQ=255)

                                                                               cATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTgc               <  2:243096/100‑1 (MQ=255)

                                                                               cATCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTgc               <  2:1015492/100‑1 (MQ=255)

                                                                                aTCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTgca              <  1:829740/100‑1 (MQ=255)

                                                                                aTCCTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTgca              >  2:492536/1‑100 (MQ=255)

                                                                                  ccTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTgcagc            >  2:112981/1‑100 (MQ=255)

                                                                                  ccTGCTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCAGAGCCCCCTTCTgcagc            >  1:2851575/1‑100 (MQ=255)

                                                                                      cTCACACAATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACg        <  1:1616057/100‑1 (MQ=255)

                                                                                      cTCACACAACCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACg        >  2:496177/1‑100 (MQ=255)

                                                                                        cacacaATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTgcagatcggaa      >  2:41415/1‑93 (MQ=255)

                                                                                        cacacaATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGt      >  2:2142643/1‑100 (MQ=255)

                                                                                        cacacaATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGt      >  1:2419170/1‑100 (MQ=255)

                                                                                         acacaTACAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTa     <  2:2551545/100‑1 (MQ=255)

                                                                                         acacaATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTa     >  2:617024/1‑100 (MQ=255)

                                                                                         acacaATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCACCCTTCTGCAGCAACGGTa     <  2:309859/100‑1 (MQ=255)

                                                                                          cacaATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTAt    <  1:1460929/100‑1 (MQ=255)

                                                                                          cacaATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTAt    <  1:874508/100‑1 (MQ=255)

                                                                                           acaATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATg   <  1:1928288/100‑1 (MQ=255)

                                                                                           acaATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATg   <  2:1774895/100‑1 (MQ=255)

                                                                                            caATCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATgg  >  2:1224713/1‑100 (MQ=255)

GGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAACCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGG  >  NC_000913/4381996‑4382187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A