Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F212 I0 R1	246	122.0	6097616	93.1%	5676880	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,564,501	T→C	25.2%	intergenic (+129/‑22)	yffR → / → yffS	CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,564,501	0	T	C	25.2%	170.8 / 67.8	111	intergenic (+129/‑22)	yffR/yffS	CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand): ref base T (46/37); new base C (10/18); total (56/55)
Fisher's exact test for biased strand distribution p-value = 8.34e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 112 total aligned reads displayed.

CACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGG  >  NC_000913/2564403‑2564600

cacCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTa                                                                                                    >  1:2258366/1‑100 (MQ=255)

cacCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGACAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACTCGTGTTAACCTCCCAATGGATTCTa                                                                                                    >  2:287264/1‑100 (MQ=255)

 acCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCAt                                                                                                   <  2:642295/100‑1 (MQ=255)

  cccGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTAt                                                                                                   <  2:2918712/99‑1 (MQ=255)

  cccGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATg                                                                                                  >  2:2428194/1‑100 (MQ=255)

  cccGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATg                                                                                                  >  1:2348845/1‑100 (MQ=255)

  cccGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATg                                                                                                  <  1:2244552/100‑1 (MQ=255)

    cGGCCCCCCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTAAGCTTACCCTCCACCAAAACGCATGTTAACCTCCCAATGGATTCTATgag                                                                                                >  2:882826/1‑100 (MQ=255)

      gCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGAt                                                                                              >  1:2225606/1‑100 (MQ=255)

       ccccTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATg                                                                                             <  2:2132447/100‑1 (MQ=255)

        cccTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATgg                                                                                            >  1:1628727/1‑100 (MQ=255)

        cccTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATgg                                                                                            >  2:899300/1‑100 (MQ=255)

        cccTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATgg                                                                                            >  2:1037053/1‑100 (MQ=255)

         ccTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCTTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATggg                                                                                           <  1:943491/100‑1 (MQ=255)

         ccTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATggg                                                                                           <  1:179582/100‑1 (MQ=255)

          cTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTCTGAGATGGga                                                                                          >  2:2921611/1‑100 (MQ=255)

           tCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGgag                                                                                         <  2:2194003/100‑1 (MQ=255)

             gCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGgaga                                                                                        <  2:860599/99‑1 (MQ=255)

             gCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGAt                                                                                       >  2:301118/1‑100 (MQ=255)

                    tttttGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATc                                                                                <  2:2392034/100‑1 (MQ=255)

                       ttGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCAta                                                                             <  1:1634477/100‑2 (MQ=255)

                       ttGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGAATTCTATGAGATGGGAGATAAAAATCAta                                                                             >  1:446688/1‑99 (MQ=255)

                        tgtgATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCAtaa                                                                            <  1:2161650/100‑3 (MQ=255)

                          tgATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCTAATGGATTCTATGAGATGGGAGATAAATATAATAAg                                                                           >  2:2474439/1‑99 (MQ=255)

                           gATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGtt                                                                         <  2:158090/100‑1 (MQ=255)

                           gATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGtt                                                                         <  2:158178/100‑1 (MQ=255)

                             tCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTAc                                                                       <  1:2759584/100‑1 (MQ=255)

                                gCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGCTAAAAATCATAAGTTACGaa                                                                    >  2:1611617/1‑100 (MQ=255)

                                gCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGaa                                                                    >  1:468098/1‑100 (MQ=255)

                                gCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGaa                                                                    <  1:1837111/100‑1 (MQ=255)

                                 cGTCAATATTCCCTTCCGTTACGTTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGaaa                                                                   <  1:1759140/100‑1 (MQ=255)

                                 cGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGaaa                                                                   <  2:506667/100‑1 (MQ=255)

                                 cGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGaa                                                                    >  2:2853092/1‑99 (MQ=255)

                                  gTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAAt                                                                  <  2:1615934/100‑1 (MQ=255)

                                   tCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAAt                                                                  >  2:720408/1‑99 (MQ=255)

                                    cAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCa                                                                >  2:557263/1‑100 (MQ=255)

                                     aaTATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCaa                                                               >  1:2668502/1‑100 (MQ=255)

                                       tatTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCaaaa                                                             <  2:2629609/100‑1 (MQ=255)

                                       tatTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCaaaa                                                             >  2:2977843/1‑100 (MQ=255)

                                        atTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAAt                                                            <  1:2363900/100‑1 (MQ=255)

                                         ttCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAAtt                                                           >  2:1412133/1‑100 (MQ=255)

                                           cccTTCCGTTACGCTTACCTTCAACAAATACGCATGTTAACCTCCCAATAAATTCTATGAGAAGGGAGATAAAAATCATAAGTTACCAAATCAAAAttt                                                          >  2:618102/1‑99 (MQ=255)

                                            ccTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGc                                                        <  2:765313/100‑1 (MQ=255)

                                            ccTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGc                                                        <  2:1314471/100‑1 (MQ=255)

                                            ccTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGc                                                        >  1:966703/1‑100 (MQ=255)

                                               tCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGAc                                                     <  1:202646/100‑1 (MQ=255)

                                                ccGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACa                                                    >  2:2502818/1‑100 (MQ=255)

                                                 cGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACAt                                                   >  1:2156874/1‑100 (MQ=255)

                                                   ttACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATcc                                                 <  2:1592489/100‑1 (MQ=255)

                                                      cGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCa                                              <  2:1808257/100‑1 (MQ=255)

                                                      cGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCa                                              <  2:2712505/100‑1 (MQ=255)

                                                       gCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCaa                                             >  2:1182843/1‑100 (MQ=255)

                                                       gCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCaa                                             <  1:1622775/100‑1 (MQ=255)

                                                        cTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGAAGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCaaa                                            >  1:117326/1‑100 (MQ=255)

                                                          tACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAgg                                          <  2:2013396/100‑1 (MQ=255)

                                                           aCCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGgc                                         <  1:2763236/100‑1 (MQ=255)

                                                            cccTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGgcg                                        >  1:619893/1‑100 (MQ=255)

                                                             ccTCCACCATTTCGCGTGTTATCCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGgc                                         <  1:1489012/98‑1 (MQ=255)

                                                             ccTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGgcgc                                       >  2:1986181/1‑100 (MQ=255)

                                                             ccTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGgcgc                                       >  2:1715950/1‑100 (MQ=255)

                                                                ccaccaATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGc                                    >  2:59700/1‑100 (MQ=255)

                                                                  accaATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGa                                  >  2:2244615/1‑100 (MQ=255)

                                                                   ccaATACGCGTGTTAACCTCGCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGAt                                 >  1:2863269/1‑100 (MQ=255)

                                                                     aaTACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATgg                               <  2:1709321/100‑1 (MQ=255)

                                                                     aaTACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATgg                               >  1:2415130/1‑100 (MQ=255)

                                                                      aTACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATgaa                              <  1:2058619/100‑3 (MQ=255)

                                                                      aTACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGa                              >  2:2151379/1‑100 (MQ=255)

                                                                       tACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGaa                             >  1:1292694/1‑100 (MQ=255)

                                                                       tACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGaa                             <  1:1258187/100‑1 (MQ=255)

                                                                        aCGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAg                            <  1:1049260/100‑1 (MQ=255)

                                                                         cgcgTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAgg                           <  1:1447912/100‑1 (MQ=255)

                                                                            gtgtTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACa                        >  2:673213/1‑100 (MQ=255)

                                                                             tgtTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACaa                       >  2:2113071/1‑100 (MQ=255)

                                                                               ttAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAata                     <  1:301121/100‑1 (MQ=255)

                                                                                 aaCCTCCCAATTGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAAtata                   <  1:207439/100‑1 (MQ=255)

                                                                                 aaCCTCCCAATGGATTCTATGAGATAGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAAtata                   >  2:2580777/1‑100 (MQ=255)

                                                                                 aaCCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAAtata                   <  1:933481/100‑1 (MQ=255)

                                                                                 aaCCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAAtata                   >  1:1929817/1‑100 (MQ=255)

                                                                                  aCCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATaa                  >  2:2016809/1‑100 (MQ=255)

                                                                                   ccTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAAGCGCTGCGATGGAAGGACAATATAAg                 >  1:1477847/1‑100 (MQ=255)

                                                                                     tCCCAATGGATTTTATGAGATGGGGGATAAAAATCATAAGTTACGATATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAggg               <  1:347234/100‑1 (MQ=255)

                                                                                     tCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAggg               <  1:2549568/100‑1 (MQ=255)

                                                                                     tCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAggg               >  2:495257/1‑100 (MQ=255)

                                                                                      cccAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGgc              >  1:2072320/1‑100 (MQ=255)

                                                                                      cccAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGgc              >  2:333580/1‑100 (MQ=255)

                                                                                      cccAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAg                 >  2:2385767/1‑97 (MQ=255)

                                                                                       ccAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGgcg             >  2:809743/1‑100 (MQ=255)

                                                                                       ccAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATACTCAAAGGCGCTGCGATGGAAGGACAATATAAGGgcg             >  2:574226/1‑100 (MQ=255)

                                                                                       ccAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACTAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGgc              <  1:1111721/99‑1 (MQ=255)

                                                                                        cAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGgcgc            >  2:2531254/1‑100 (MQ=255)

                                                                                        cAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGgcgc            >  1:2833304/1‑100 (MQ=255)

                                                                                          aTGGATTCTATTAGATGGGACATAAATATCATTAGTTACGAAATCATAATTTGCGACTTCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTc          <  1:2474468/100‑1 (MQ=255)

                                                                                          aTGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTc          <  1:1862796/100‑1 (MQ=255)

                                                                                           tGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCa         <  2:2299719/100‑1 (MQ=255)

                                                                                             gATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAAc       >  1:827751/1‑100 (MQ=255)

                                                                                             gATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAAc       <  1:1492371/100‑1 (MQ=255)

                                                                                              aTTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACg      <  2:2984313/100‑1 (MQ=255)

                                                                                                 cTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTgg   >  1:2147549/1‑100 (MQ=255)

                                                                                                 cTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTgg   <  2:954061/100‑1 (MQ=255)

                                                                                                  tATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGAAGGAAGGACAATATAAGGCCGCTCAACGTggg  <  1:2042053/100‑1 (MQ=255)

CACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGG  >  NC_000913/2564403‑2564600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A