Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F212 I0 R1 246 122.0 6097616 93.1% 5676880 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,188,787 A→T 34.0% E1146D (GAA→GAT)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,188,7870AT34.0% 155.5 / 124.6 134E1146D (GAA→GAT) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base A (53/34);  new base T (30/15);  total (84/50)
Fisher's exact test for biased strand distribution p-value = 5.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

AGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCC  >  NC_000913/4188690‑4188885
                                                                                                 |                                                                                                  
aGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGTGGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGc                                                                                                  <  1:1100508/100‑1 (MQ=255)
aGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGc                                                                                                  <  2:1273676/100‑1 (MQ=255)
aGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGc                                                                                                  >  1:544220/1‑100 (MQ=255)
 gATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCa                                                                                                 >  1:2768830/1‑100 (MQ=255)
   tCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGATGCAcg                                                                                               >  1:877291/1‑100 (MQ=255)
      gCTCTGGTGACACGCTGGCGCGTATTCCGCAGGAATCCAGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGATGCAcgtcg                                                                                            >  2:1871289/1‑100 (MQ=255)
      gCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGGGTTGCGGACCTGTTCGAAGCAcgtcg                                                                                            >  2:712982/1‑100 (MQ=255)
      gCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCAcgtcg                                                                                            >  1:1562131/1‑100 (MQ=255)
         ctGGTGACACCCTGGCGCGTATTCCGCAGGATTCTGGTGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGACGCACGTCGTcc                                                                                         <  1:350965/100‑1 (MQ=255)
         ctGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGATGCACGTCGTcc                                                                                         >  1:1866463/1‑100 (MQ=255)
           ggTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGATGCACGTCGTCCGa                                                                                       >  2:2061661/1‑100 (MQ=255)
           ggTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGAACTGTTCGATGCACGTCGTCCGa                                                                                       >  1:1163078/1‑100 (MQ=255)
            gTGACCCCCTGGCGCGTATTCAGAAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGCTGCGGACCTGTTCGATGCACGTCGTCCg                                                                                        >  2:1484389/1‑98 (MQ=255)
             tGACTCCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGATGCACGTCGTCCaaa                                                                                      <  2:1904323/99‑1 (MQ=255)
             tGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGATGCACGTCGTCCGaaa                                                                                     <  1:976648/100‑1 (MQ=255)
             tGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGaa                                                                                      >  2:444724/1‑99 (MQ=255)
                cacCCTGGCGGGTATTCCGCAGGAATCCGGCGGTACCGAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGTCCTGTTCGATGCACGTCGTCCGaa                                                                                      <  2:1981331/96‑1 (MQ=255)
                cacCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGATGCACGTCGTCCGAAagag                                                                                  >  2:2483231/1‑100 (MQ=255)
                cacCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAagag                                                                                  >  2:281823/1‑100 (MQ=255)
                cacCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAagag                                                                                  >  2:2402786/1‑100 (MQ=255)
                cacCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAagag                                                                                  >  2:119229/1‑100 (MQ=255)
                cacCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAagag                                                                                  <  1:2736444/100‑1 (MQ=255)
                  cccTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGATGCACGTCGTCCGAAAGAGcc                                                                                >  2:1132044/1‑100 (MQ=255)
                  cccTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGcc                                                                                >  1:80419/1‑100 (MQ=255)
                  cccTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGcc                                                                                >  1:1412842/1‑100 (MQ=255)
                  cccTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGcc                                                                                <  1:2686704/100‑1 (MQ=255)
                    cTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCgg                                                                              <  1:1734403/100‑1 (MQ=255)
                     tGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGc                                                                             <  1:1730994/100‑1 (MQ=255)
                     tGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGc                                                                             <  1:444727/100‑1 (MQ=255)
                        cgcgTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAAt                                                                          >  2:2672530/1‑100 (MQ=255)
                         gcgTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGATGCACGTCGTCCGAAAGAGCCGGCAATc                                                                         <  1:1077949/100‑1 (MQ=255)
                           gTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATc                                                                         >  2:1863535/1‑98 (MQ=255)
                           gTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCt                                                                       >  1:665326/1‑100 (MQ=255)
                             aTTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTAGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTgg                                                                     >  2:2779288/1‑100 (MQ=255)
                              ttCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGATGCACGTCGTCCGAAAGAGCCGGCAATCCTGGc                                                                    <  1:1815708/100‑1 (MQ=255)
                              ttCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGc                                                                    >  2:2193260/1‑100 (MQ=255)
                              ttCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGc                                                                    <  2:2765561/100‑1 (MQ=255)
                              ttCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAACACGTCGTCCGAAAGAGCCGGCAATCCTGGc                                                                    >  1:2450661/1‑100 (MQ=255)
                               tCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGATGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCt                                                                   <  1:43081/100‑1 (MQ=255)
                                ccGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTg                                                                  >  1:1236952/1‑100 (MQ=255)
                                ccGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTg                                                                  >  2:1431126/1‑100 (MQ=255)
                                ccGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTg                                                                  >  1:2349830/1‑100 (MQ=255)
                                ccGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAACACGACATCAGAAAGAGCCGGCAAACATGGCTg                                                                  >  1:529169/1‑100 (MQ=255)
                                 cGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGa                                                                 >  2:330193/1‑100 (MQ=255)
                                 cGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTAGAAACACAAAGAACGAAAAAGCCGGCAATCCTGGCTGa                                                                 >  1:38780/1‑100 (MQ=255)
                                   cAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGATGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGaaa                                                               >  2:505548/1‑100 (MQ=255)
                                     ggAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGATGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATc                                                             >  1:2958776/1‑100 (MQ=255)
                                       aaTCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAg                                                           >  2:1972504/1‑100 (MQ=255)
                                        aTCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGACGGCAATCCTGGCTGAAATCAGc                                                          >  1:1040533/1‑100 (MQ=255)
                                         tCCGGCGGTACCAAGGACATCACCGGTGGTCTTCCGCGCATTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCg                                                         <  2:2131017/100‑1 (MQ=255)
                                         tCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGATGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCg                                                         >  1:732418/1‑100 (MQ=255)
                                          ccGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCgg                                                        <  1:2034030/100‑1 (MQ=255)
                                              cggTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATc                                                    <  1:1956407/100‑1 (MQ=255)
                                               ggTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGATGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCg                                                   >  2:1790352/1‑100 (MQ=255)
                                                  aCCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGttt                                                >  1:504364/1‑100 (MQ=255)
                                                  aCCAAGGACATCACCGGTGGTCTGCCGCGCGTTACGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGttt                                                >  2:2285331/1‑100 (MQ=255)
                                                   ccAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGtt                                                 >  2:1782956/1‑98 (MQ=255)
                                                   ccAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTc                                               >  1:512814/1‑100 (MQ=255)
                                                    cAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGATGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTcc                                              <  1:912299/100‑1 (MQ=255)
                                                    cAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTcc                                              >  1:2807549/1‑100 (MQ=255)
                                                     aaGGACATCACCGGTGGTCTGCAGCGCATTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAACCGGCAATACTGGCTGAAATCAGCGGTAGCGTTccc                                              >  2:475083/1‑99 (MQ=255)
                                                       ggACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGATGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTc                                           >  2:1775774/1‑100 (MQ=255)
                                                         tccgatctggtggtCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCgg                                         <  2:1070961/92‑1 (MQ=255)
                                                         aCATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGATGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCgg                                         >  2:1583206/1‑100 (MQ=255)
                                                           aTCACCGGTGGTCTGCCGCGCGTGGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCGGGCAATCCTGGCTGAAATCAGCGGTATCGTGTCCTTCGGTa                                       <  1:1861221/100‑1 (MQ=255)
                                                            tCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGATGCAAGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTaa                                      >  2:1655571/1‑100 (MQ=255)
                                                             cacaGGTGGCCTGCCGCGCGTTGCGGATCTGTTCTAAGCACGTCGACCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGGATCGATTCCTTCGGTaa                                      <  2:825927/99‑1 (MQ=255)
                                                             cACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGATGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTaaa                                     >  2:1149622/1‑100 (MQ=255)
                                                              aCCGGTGGTCTGCCGCGCGTTGCGGTCCTGTTTGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAg                                    <  1:2498197/100‑1 (MQ=255)
                                                               ccGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGa                                   <  1:2402814/100‑1 (MQ=255)
                                                                 ggtggtCTGCCGCGCGTTGCGGACCTGTTCGATGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGAGGTATCGTTTCCTTCGATAAAGaaa                                 >  1:1343546/1‑100 (MQ=255)
                                                                 ggtggtCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCggagatcgga                                 >  1:1070968/1‑92 (MQ=255)
                                                                   tggtCTGCCGCGCGTTGCGGACCTGTTCGATGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAAcc                               >  2:2256435/1‑100 (MQ=255)
                                                                     gtCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCaa                             >  1:2709165/1‑100 (MQ=255)
                                                                     gtCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCaa                             >  1:2566681/1‑100 (MQ=255)
                                                                       cTGCCGCGCGTTGCGGACCTGTTCGATGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCaatg                           <  1:2061684/100‑3 (MQ=255)
                                                                        tGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAgg                          >  1:2079156/1‑100 (MQ=255)
                                                                        tGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAgg                          <  2:1045841/100‑1 (MQ=255)
                                                                         gCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGt                         >  2:7304/1‑100 (MQ=255)
                                                                         gCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGt                         >  2:2826443/1‑100 (MQ=255)
                                                                          ccGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTa                        >  1:25697/1‑100 (MQ=255)
                                                                            gcgcgTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTaaa                      <  1:2271942/100‑1 (MQ=255)
                                                                                  tGCGGACCTGTTCGATGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAAcgtcgt                >  1:875008/1‑100 (MQ=255)
                                                                                  tGCGGACCTGTTCGATGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAAcgtcgt                >  2:2806216/1‑100 (MQ=255)
                                                                                  tGCGGACCTGTTCGATGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAAcgtcgt                <  1:2566117/100‑1 (MQ=255)
                                                                                  tGCGGACCTGTTCGATGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGCTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAAcgtcgt                >  1:2858039/1‑100 (MQ=255)
                                                                                  tGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAAcgtcgt                >  1:388132/1‑100 (MQ=255)
                                                                                    cGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCt              >  2:2791737/1‑100 (MQ=255)
                                                                                     ggACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTg             <  2:573058/100‑1 (MQ=255)
                                                                                       aCCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTGCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGt           <  1:2139077/100‑1 (MQ=255)
                                                                                        ccTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGtt          <  2:388129/100‑1 (MQ=255)
                                                                                          tGTTCGACGCACGTCGTCCCAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAACCCAAAGGTAAACGTCGCCTGGttgt        >  2:746127/1‑98 (MQ=255)
                                                                                          tGTTCGAAGCACGTCGTTCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTAt        <  1:1458013/100‑1 (MQ=255)
                                                                                           gTTCGATGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATc       <  2:2850865/100‑1 (MQ=255)
                                                                                           gTTCGATGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATc       >  1:300053/1‑100 (MQ=255)
                                                                                           gTTCGATGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATc       <  1:1922270/100‑1 (MQ=255)
                                                                                           gTTCGATACACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTCCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATc       <  2:1620491/100‑1 (MQ=255)
                                                                                           gTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTAt        >  2:1455700/1‑99 (MQ=255)
                                                                                              cGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATAAcc    >  2:1289746/1‑100 (MQ=255)
                                                                                              cGAAGCACGTCGTACGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCAcc    >  2:1964307/1‑100 (MQ=255)
                                                                                                 |                                                                                                  
AGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCC  >  NC_000913/4188690‑4188885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A