Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F204 I0 R1 247 174.3 8556966 94.2% 8060661 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,602,188 T→C 42.8% I233I (ATT→ATC)  lsrA → autoinducer 2 import ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,602,1880TC42.8% 40.9 / 133.2 112I233I (ATT→ATC) lsrAautoinducer 2 import ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (35/29);  new base C (28/20);  total (63/49)
Fisher's exact test for biased strand distribution p-value = 8.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-01

Only 100 of 115 total aligned reads displayed.

CCGGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACG  >  NC_000913/1602090‑1602287
                                                                                                  |                                                                                                   
ccGGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTc                                                                                                    >  1:1407458/1‑100 (MQ=255)
ccGGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTc                                                                                                    <  2:3795014/100‑1 (MQ=255)
 cGGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCa                                                                                                   >  2:159492/1‑100 (MQ=255)
 cGGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTATCTACCGACGACATTATTCa                                                                                                   >  1:1805731/1‑100 (MQ=255)
  ggAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAg                                                                                                  >  1:3569891/1‑100 (MQ=255)
     aaTTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATCCAGGcc                                                                                               >  2:3067462/1‑100 (MQ=255)
       ttCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCAt                                                                                             <  2:2675297/100‑1 (MQ=255)
        tCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGCCGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATc                                                                                            >  1:1648045/1‑100 (MQ=255)
        tCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATc                                                                                            <  2:1289763/100‑1 (MQ=255)
         cGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCa                                                                                           <  2:1060322/100‑1 (MQ=255)
           ccAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCAcc                                                                                         <  1:2204915/100‑1 (MQ=255)
              gATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATCCAGGCCATCACCCCa                                                                                      <  2:2990403/100‑1 (MQ=255)
                   ccGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATCCAGGCCATCACCCCAGCGGt                                                                                 <  2:3355054/100‑1 (MQ=255)
                   ccGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATCCAGGCCATCACCCCAGCGGt                                                                                 >  2:1990664/1‑100 (MQ=255)
                   ccGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATCCAGGCCATCACCCCAGCGGt                                                                                 >  1:4145785/1‑100 (MQ=255)
                    cGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTa                                                                                >  2:1616053/1‑100 (MQ=255)
                    cGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATCCAGGCCATCACCCCAGCGGTa                                                                                >  2:2413115/1‑100 (MQ=255)
                    cGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATCCAGGCCATCACCCCAGCGGTa                                                                                >  1:3097714/1‑100 (MQ=255)
                    cGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATCCAGGCCATCACCCCAGCGGTa                                                                                <  2:3382738/100‑1 (MQ=255)
                      aTCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACg                                                                              <  1:3219854/100‑1 (MQ=255)
                       tCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACgg                                                                             <  2:3143043/100‑1 (MQ=255)
                         gAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATCCAGGCCATCACCCCAGCGGTACggg                                                                            >  1:2349693/1‑99 (MQ=255)
                         gAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATCCAGGCCATCACCCCAGCGGTACGGGa                                                                           >  2:969944/1‑100 (MQ=255)
                           ataaGCGTCATGCGCGACGGAACCAGCGCCTTAAGCGGCAACACCAGCGAACTGTCTACCGACGACATTAGCCAGGCCATCACCCCAGCAGTACGGGaaa                                                                         <  1:1705121/97‑1 (MQ=255)
                              aGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAAt                                                                      >  2:1815678/1‑100 (MQ=255)
                               gCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATCCAGGCCATCACCCCAGCGGTACGGGAAAAATc                                                                     >  1:95197/1‑100 (MQ=255)
                                cGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCGACCGACGACATTATTCAGGCCATCACCCCAGCGGTATGGGAAAAATCg                                                                    <  1:833620/100‑1 (MQ=255)
                                 gTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGc                                                                   >  2:4263500/1‑100 (MQ=255)
                                 gTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATCCAGGCCATCACCCCAGCGGTACGGGAAAAATCGc                                                                   <  2:2581943/100‑1 (MQ=255)
                                  tGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGct                                                                  >  1:1031597/1‑100 (MQ=255)
                                     tGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGctctc                                                               >  2:1798185/1‑100 (MQ=255)
                                     tGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGctctc                                                               >  1:1593174/1‑100 (MQ=255)
                                     tGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGctctc                                                               >  2:383429/1‑100 (MQ=255)
                                      gcgcGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCAT‑‑CCCCAGCGGTACGGGAAAAATCGCTCTCTgc                                                            >  1:4096384/1‑100 (MQ=255)
                                      gcgcGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCAT‑‑CCCCAGCGGTACGGGAAAAATCGCTCTCTgc                                                            >  1:4096669/1‑100 (MQ=255)
                                        gcgACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATCCAGGCCATCACCCCAGCGGTACGGG‑AAAATCGCTCTCTgcc                                                           >  2:2498256/1‑100 (MQ=255)
                                         cgACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTgcc                                                           >  2:3179402/1‑100 (MQ=255)
                                          gACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTgcc                                                           >  1:1369490/1‑99 (MQ=255)
                                             ggAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTgccagcc                                                       >  1:3092027/1‑100 (MQ=255)
                                             ggAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATCCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTgccagcc                                                       <  2:1662841/100‑1 (MQ=255)
                                              naaCCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATCCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTgccagcca                                                      >  1:1572160/2‑100 (MQ=255)
                                              gAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACGGACGACATTATCCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTgccagcca                                                      >  1:3463003/1‑100 (MQ=255)
                                              gAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATCCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTgccagcca                                                      >  2:3943517/1‑100 (MQ=255)
                                                 ccATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCaaaa                                                   >  1:1080425/1‑100 (MQ=255)
                                                 acATCGCCTTAAACGGCAAAACCAGCGAACTGTCTACCGACGACATTAGTCAGGCCAGCACCCCAGCGGTACGTGAAAAATCGCTCTCTGCCAGCCgaaa                                                   >  1:3563234/2‑100 (MQ=255)
                                                     cGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTa                                               <  2:3076473/100‑1 (MQ=255)
                                                     cGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTa                                               >  2:1637038/1‑100 (MQ=255)
                                                     cGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATAGCTCTCTGCCAGCCAAAAATTa                                               >  1:525022/1‑100 (MQ=255)
                                                      gCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTAt                                              >  2:1452269/1‑100 (MQ=255)
                                                      gCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATCCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTAt                                              >  2:3617868/1‑100 (MQ=255)
                                                      gCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATCCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTAt                                              >  1:543405/1‑100 (MQ=255)
                                                      gCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATCCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTAt                                              <  2:1181940/100‑1 (MQ=255)
                                                        cTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATCCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAACAATTATg                                             >  1:2763347/1‑99 (MQ=255)
                                                         ttAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGc                                           <  2:2281003/100‑1 (MQ=255)
                                                          tAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATCCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCt                                          >  2:1608057/1‑100 (MQ=255)
                                                           aaGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATCCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTg                                         <  1:4100319/100‑1 (MQ=255)
                                                            aGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTGAGGCCATCACCCACGCGGTACGCGGAAAATCGCTCTCTGCCAGCCACAAatgatg                                             >  1:949151/1‑95 (MQ=255)
                                                            aGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATCCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTgg                                        <  2:3533654/100‑1 (MQ=255)
                                                             gCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCATAATTTctggctggc                                       <  2:3558962/100‑2 (MQ=255)
                                                             gCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCATAATTTTTGGCTggc                                       <  1:332205/100‑2 (MQ=255)
                                                             gCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCATAATTTTTGGCTggc                                       <  2:3093875/100‑2 (MQ=255)
                                                             gCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCATAATTTTTGGCTggc                                       <  2:3980151/100‑2 (MQ=255)
                                                             gCGGCAAAACCAGCGAACTGTCTACCGACGACATTATCCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTg                                         >  1:1675142/1‑98 (MQ=255)
                                                              cGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCATAATTTTTGGCTggc                                       <  1:1716029/99‑2 (MQ=255)
                                                              cGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAg                                      <  2:4142882/100‑1 (MQ=255)
                                                              cGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAg                                      <  2:1095715/100‑1 (MQ=255)
                                                              cGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAg                                      <  1:2512522/100‑1 (MQ=255)
                                                               ggCAAAGTCGGCGAACTGTCTGCGGACGACATTATTCAGCCCATCGCCCCAGCGGTACGGGAAAAATCGCTCTCCGCTAGCCAGAATTTGTGGCAggcag                                     <  1:687001/100‑4 (MQ=255)
                                                               ggCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCATAATTTTTGGCTggcag                                     <  2:982947/100‑4 (MQ=255)
                                                                gCAAAACCAGCGAACTGTCTACCGACGACATTATCCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGtt                                    >  1:2273779/1‑100 (MQ=255)
                                                                 cAAAACCAGCGAACTGTCTACCGACGACATTATCCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAACTATGGCTGGAGtt                                    >  1:1815415/1‑99 (MQ=255)
                                                                  aaaaCCAGCGAACTGTCTACCGACGACATTATCCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTAc                                  >  1:4092165/1‑100 (MQ=255)
                                                                   aaaCCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTAcc                                 <  1:238946/100‑1 (MQ=255)
                                                                     aCCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTg                               >  2:1894972/1‑100 (MQ=255)
                                                                       cAGCGAACTGTCTACCGACGACATTATCCGGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGt                             <  1:3342713/100‑1 (MQ=255)
                                                                               tGTCTACCGACGACATTATCCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGccc                     <  2:89960/100‑1 (MQ=255)
                                                                                gTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCca                    >  2:3677850/1‑100 (MQ=255)
                                                                                gTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCca                    >  1:3203966/1‑100 (MQ=255)
                                                                                 tctcCCGACGACATTATCCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCcac                   <  1:2134556/100‑1 (MQ=255)
                                                                                 tCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCcac                   >  2:1577257/1‑100 (MQ=255)
                                                                                  cTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCcaca                  >  2:3803654/1‑100 (MQ=255)
                                                                                  cTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCcaca                  <  1:3327705/100‑1 (MQ=255)
                                                                                    aCCGACGACATTATCCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCacaac                >  1:2073173/1‑100 (MQ=255)
                                                                                    aCCGACGACATTATCCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCacaac                <  2:2007061/100‑1 (MQ=255)
                                                                                    aCCGACGACATTATCCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCacaac                >  1:4184717/1‑100 (MQ=255)
                                                                                    aCCGACGACATTATCCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCacaac                <  2:2008054/100‑1 (MQ=255)
                                                                                     ccGACGACATTATCCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCacaaca               >  1:1223878/1‑100 (MQ=255)
                                                                                      cgacgaCATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACAt              <  1:854341/100‑1 (MQ=255)
                                                                                       gacgacATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATg             >  1:2654644/1‑100 (MQ=255)
                                                                                       gacgacATTATCCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATg             <  2:543407/100‑1 (MQ=255)
                                                                                        acgacATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATgc            >  1:497855/1‑100 (MQ=255)
                                                                                        acgacATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATgc            <  2:2573043/100‑1 (MQ=255)
                                                                                         cgacATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATgcc           <  1:3653921/100‑1 (MQ=255)
                                                                                           acATTATCCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATgccgc         >  1:4023364/1‑100 (MQ=255)
                                                                                            cATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCACAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATgccgcc        >  1:2000857/1‑100 (MQ=255)
                                                                                            cATTATCCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATgccgcc        >  2:4075014/1‑100 (MQ=255)
                                                                                            cATTATCCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATgccgcc        <  2:1691926/100‑1 (MQ=255)
                                                                                             attattCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGccgccg       >  2:3903175/1‑100 (MQ=255)
                                                                                             attatcCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGccgccg       >  2:3544904/1‑100 (MQ=255)
                                                                                                  tCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACg  >  2:2494197/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
CCGGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACG  >  NC_000913/1602090‑1602287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A