Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F204 I0 R1 247 174.3 8556966 94.2% 8060661 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,134,852 A→G 53.8% intergenic (+29/+20) yghT → / ← pitB putative ATP‑binding protein/phosphate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,134,8520AG53.8% 52.6 / 175.7 150intergenic (+29/+20)yghT/pitBputative ATP‑binding protein/phosphate transporter
Reads supporting (aligned to +/- strand):  ref base A (32/37);  new base G (45/35);  total (78/72)
Fisher's exact test for biased strand distribution p-value = 2.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 151 total aligned reads displayed.

TGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATCA  >  NC_000913/3134756‑3134951
                                                                                                |                                                                                                   
tGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCg                                                                                                  >  2:72632/1‑100 (MQ=255)
tGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACCGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAGATGGAAATGCCCGATCGCCAGGACCg                                                                                                  <  2:342984/100‑1 (MQ=255)
  gAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCggg                                                                                                <  2:1578552/100‑1 (MQ=255)
  gAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCggg                                                                                                <  1:2085618/100‑1 (MQ=255)
  gAACAGCTTTTGGTAGATGCAGAAAAAGCGATTACGTATTTTATGACCGCGCGTGGTTATCGCTATTCAAAAATGGAAATGCCCCATCGCCAGGGCCgag                                                                                                <  1:596160/100‑3 (MQ=255)
   aataGGTGGTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGc                                                                                               <  1:340459/97‑1 (MQ=255)
   aaCAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGc                                                                                               <  2:1740113/100‑1 (MQ=255)
   aaCAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGc                                                                                               >  2:298412/1‑100 (MQ=255)
     cAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCAt                                                                                             <  2:2536951/100‑1 (MQ=255)
      aGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCAtt                                                                                            >  1:1341301/1‑100 (MQ=255)
      aGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCAtt                                                                                            >  1:183922/1‑100 (MQ=255)
       ggTGTTGGTTGCTGCAGAAAAAGCGATTACGCAGTTTATGACCGCGCGTGGATATCACTAGTCAAAAATAGAAATGCCCGATCGCCACGACCGGGccat                                                                                            >  1:380295/1‑96 (MQ=255)
         tgtTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTc                                                                                         >  2:1407247/1‑100 (MQ=255)
         tgtTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTc                                                                                         <  1:2246130/100‑1 (MQ=255)
          gttggttgATGCAGAAACAGCGACTACGGATTTTATGACCGCGCGTGGTTATCACTAGTTAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCa                                                                                        >  1:229522/1‑100 (MQ=255)
           ttggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAg                                                                                       <  2:943673/100‑1 (MQ=255)
           ttggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAg                                                                                       <  1:2825402/100‑1 (MQ=255)
            tggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAgg                                                                                      <  2:3732971/100‑1 (MQ=255)
             ggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGa                                                                                     >  2:666761/1‑100 (MQ=255)
             agttgATGCAGAAAAAGAGATTACGGATTTTATGACGGCGCGTGGTTATCACGAGTTAAAAATAGTAATGCCCGATCGCCAGGGCAGGGCATTCTCAgg                                                                                      >  1:3562366/2‑99 (MQ=255)
              gttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGaa                                                                                    >  2:3239632/1‑100 (MQ=255)
              gttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGaa                                                                                    >  1:1020399/1‑100 (MQ=255)
                tgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAgg                                                                                  <  2:2640236/100‑1 (MQ=255)
                  aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGtt                                                                                <  2:3915600/100‑1 (MQ=255)
                   tGCAGAAAAAGCGATTACGGATTTTATGGCCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTa                                                                               <  2:1008524/100‑1 (MQ=255)
                   tGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTa                                                                               <  2:1187241/100‑1 (MQ=255)
                   tGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTa                                                                               >  1:685797/1‑100 (MQ=255)
                   tGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGGTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGtt                                                                                >  2:2970726/1‑99 (MQ=255)
                    gCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTaa                                                                              >  1:1635376/1‑100 (MQ=255)
                      aGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAAt                                                                            <  2:2868940/100‑1 (MQ=255)
                      aGAAAAAGCGATGACGGATTATATGACCCCGCGTGGTCATCACTAGTCTAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAAt                                                                            <  1:47185/100‑1 (MQ=255)
                       gAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATc                                                                           <  1:584979/100‑1 (MQ=255)
                         aaaaGCGATTACGGAATTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCaa                                                                         >  2:4174650/1‑100 (MQ=255)
                             gCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGc                                                                     <  2:1456180/100‑1 (MQ=255)
                              cGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCa                                                                    <  2:865953/100‑1 (MQ=255)
                              cGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCa                                                                    <  2:750251/100‑1 (MQ=255)
                               gATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCaa                                                                   >  2:1022433/1‑100 (MQ=255)
                                aTTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAAt                                                                  <  2:3900520/100‑1 (MQ=255)
                                aTTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAAt                                                                  <  2:3765059/100‑1 (MQ=255)
                                aTTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAAt                                                                  <  1:121803/100‑1 (MQ=255)
                                 ttACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                 <  1:3308438/100‑1 (MQ=255)
                                  tACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGc                                                                <  1:337712/100‑1 (MQ=255)
                                  tACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGc                                                                <  2:2291375/100‑1 (MQ=255)
                                  tACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGc                                                                <  2:3543800/100‑1 (MQ=255)
                                    cGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTa                                                              >  1:3101974/1‑100 (MQ=255)
                                    cGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTa                                                              >  2:2139722/1‑100 (MQ=255)
                                    cGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTa                                                              >  1:1443568/1‑100 (MQ=255)
                                      gATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATc                                                            >  2:3559319/1‑100 (MQ=255)
                                       aTTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATcc                                                           <  1:72632/100‑1 (MQ=255)
                                        ttttATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCa                                                          >  1:2746905/1‑100 (MQ=255)
                                           tATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTa                                                       >  2:3231108/1‑100 (MQ=255)
                                            aTGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTAc                                                      <  1:1300808/100‑1 (MQ=255)
                                            aTGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTAc                                                      <  2:735041/100‑1 (MQ=255)
                                            aTGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTAc                                                      <  2:1899874/100‑1 (MQ=255)
                                               aCCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGc                                                   <  2:3193441/100‑1 (MQ=255)
                                               aCCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCGGTACAGc                                                   <  1:662753/100‑1 (MQ=255)
                                                 cgcgcgTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGccc                                                 >  2:4167374/1‑100 (MQ=255)
                                                    gcgtgtgtgtCACGAGTCAAAAATGGAAATGCCGTAGCGCCAGAACCGGGAAGTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGcc                                                  <  1:3254041/87‑1 (MQ=255)
                                                    gcgTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCccacc                                              <  1:440683/100‑1 (MQ=255)
                                                    gcgTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCccacc                                              <  1:2506465/100‑1 (MQ=255)
                                                    gcgTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCccacc                                              <  1:3029278/100‑1 (MQ=255)
                                                     cgTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCccacca                                             >  2:1711864/1‑100 (MQ=255)
                                                      gTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCag                                            >  2:1324705/1‑100 (MQ=255)
                                                        ggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaa                                          >  2:3135531/1‑100 (MQ=255)
                                                         gTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaaa                                         <  2:2125755/100‑1 (MQ=255)
                                                            aTCACTAGTCAAAAATGGAAATGCCCGATCGCAAGGACCGGGCATTTTCAGGAAGGTTAACTCAACTGCAATGCTATCCAGTACAGCCCACCagaaag                                        <  2:1909874/98‑1 (MQ=255)
                                                               aCTAGTCAAAAATGGAAATGCCCGATCGCCAGGTCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGaaaaa                                   >  2:3301627/1‑100 (MQ=255)
                                                               aCTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGaaaaa                                   <  2:3539413/100‑1 (MQ=255)
                                                               aCTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGaaaaa                                   >  2:3262738/1‑100 (MQ=255)
                                                                 tAGTCAAAAATGGAAATGAGCGATCGCCAGGACCGGGAATTTTAAGGGAGGTTAAATGAACTGCAATGCTGTCGAGTACAGCTGACGAGAAAGATAAAtt                                 >  1:2544073/1‑100 (MQ=255)
                                                                  aGTCAAAAATGGATATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTg                                <  2:2350395/100‑1 (MQ=255)
                                                                   gtgaaaaaTGGAAATGGCCGATCGCCACGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAAGTgc                               <  1:667019/97‑1 (MQ=255)
                                                                   gTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgc                               >  1:2143889/1‑100 (MQ=255)
                                                                     cAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgccg                             >  1:1457317/1‑100 (MQ=255)
                                                                     cAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgccg                             >  1:1230048/1‑100 (MQ=255)
                                                                     cAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgccg                             >  2:4076934/1‑100 (MQ=255)
                                                                     cAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgccg                             >  1:2685142/1‑100 (MQ=255)
                                                                     cAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgccg                             >  1:4093023/1‑100 (MQ=255)
                                                                     cAAAAATAGAAATGCCCGATCGCCAGGGCCGGGCATATTCAGGAAGGATAAATCAACTGCAATGCTATCCACTACAGCCCGCCAGAAAGAAAAATTgcc                              >  1:409374/1‑99 (MQ=255)
                                                                      aaaaaTGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGCAAAATTgccgc                            >  2:1729359/1‑100 (MQ=255)
                                                                         aaTGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCgg                         <  1:3864338/100‑1 (MQ=255)
                                                                          agggAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgt                        <  1:2094756/98‑1 (MQ=255)
                                                                            ggAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgtaa                      <  2:2916010/100‑1 (MQ=255)
                                                                            ggAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATAAACTGCAATGCTATCCAGTACAGAAAAAAAAAAAAAAAAATTGCCGCCGgtaa                      <  1:3669011/100‑1 (MQ=255)
                                                                             gAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgtaaa                     >  2:3032306/1‑100 (MQ=255)
                                                                              aaaTGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgtaaag                    >  2:2907199/1‑100 (MQ=255)
                                                                              aaaTGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgtaaag                    <  2:3993620/100‑1 (MQ=255)
                                                                              aaaTGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgtaaag                    <  1:4199336/100‑1 (MQ=255)
                                                                               aaTGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgtaaagt                   >  1:1353227/1‑100 (MQ=255)
                                                                                    ccGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATa              >  2:935812/1‑100 (MQ=255)
                                                                                    ccGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATa              >  2:3090475/1‑100 (MQ=255)
                                                                                    ccGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATa              >  1:2898404/1‑100 (MQ=255)
                                                                                     cGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATAc             >  2:2849491/1‑100 (MQ=255)
                                                                                        tCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCa          <  2:3474931/100‑1 (MQ=255)
                                                                                          gCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACg        >  1:4272020/1‑100 (MQ=255)
                                                                                          gCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACg        <  2:1525840/100‑1 (MQ=255)
                                                                                          gCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAACAAAAATTGCCGCCGGAACAGTCAATACCCAc         >  1:2341070/1‑99 (MQ=255)
                                                                                          gCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTAAAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGTCGGTAAAGTAAATACCCACg        <  1:2958961/100‑1 (MQ=255)
                                                                                               gACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATCCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATc   <  1:2880164/100‑1 (MQ=255)
                                                                                                aCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATCa  <  2:3658021/100‑1 (MQ=255)
                                                                                                |                                                                                                   
TGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATCA  >  NC_000913/3134756‑3134951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A