Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F204 I0 R1	247	174.3	8556966	94.2%	8060661	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,658,780	A→G	40.4%	T139A (ACG→GCG)	gadE →	gad regulon transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,658,780	0	A	G	40.4%	111.5 / 205.6	188	T139A (ACG→GCG)	gadE	gad regulon transcriptional activator
Reads supporting (aligned to +/- strand): ref base A (55/56); new base G (29/46); total (86/102)
Fisher's exact test for biased strand distribution p-value = 1.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

Only 100 of 189 total aligned reads displayed.

CTTGCCCCATAAGAATTCACAATTATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATC  >  NC_000913/3658683‑3658878

cTTGCCCCATAAGAATTCACAATTATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACg                                                                                                  >  2:2441820/1‑100 (MQ=255)

  tGCCCCATAAGAATTCACAATTATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCt                                                                                                <  2:686664/100‑1 (MQ=255)

   gCCCCATAAGAATTCACAATTATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTc                                                                                               <  2:1415503/100‑1 (MQ=255)

    ccccATAAGAATTCACAATTATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCa                                                                                              <  2:2057775/100‑1 (MQ=255)

     cccATAAGAATTCACAATTATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGGCGCTCaa                                                                                             <  2:2489682/100‑1 (MQ=255)

     cccATAAGAATTCACAATTATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCaa                                                                                             >  1:367084/1‑100 (MQ=255)

        aTAAGAATTCACAATTATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGGCGCTCAatat                                                                                          <  1:1157391/100‑1 (MQ=255)

         tAAGAATTCACAATTATGTTTTTCACATAATCAGTTCAAAATTATACAACTGATTCTGAAAAATAAATATGAAAGCAATATCACATCGACGCTCAATAtt                                                                                         >  1:1785574/1‑100 (MQ=255)

          aaaaaTTCACAATTATGTTTTACACATAATCAGTTCAAAATTATGCAACTGAATAAAAAAAAAAAAAATGAAAGCAATATCACGTCGGCGCTCAATAttt                                                                                        <  1:2406373/97‑1 (MQ=255)

           aGAATTCACAATTATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTTAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAAAAttt                                                                                        >  1:54358/1‑99 (MQ=255)

             aaTTCACAATTATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGc                                                                                     <  2:276802/100‑1 (MQ=255)

             aaTTCACAATTATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGc                                                                                     <  1:3795468/100‑1 (MQ=255)

             aaTTCACAATTATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGc                                                                                     <  1:249488/100‑1 (MQ=255)

             aaTTCACAATTATGTTTTTCACATAATCAATTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGGCGCTCAATATTTCGc                                                                                     <  1:875206/100‑1 (MQ=255)

              aTTCACAATTATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGca                                                                                    <  2:4177220/100‑1 (MQ=255)

               ttCACAATTATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGGCGCTCAATATTTCGcaa                                                                                   >  2:3443351/1‑100 (MQ=255)

                tCACAATTATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGcaac                                                                                  >  2:2841593/1‑100 (MQ=255)

                 cacaATTATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGGCGCTCAATATTTCGcaaca                                                                                 >  1:2074718/1‑100 (MQ=255)

                 cacaATTATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGGCGCTCAATATTTCGcaaca                                                                                 >  2:213419/1‑100 (MQ=255)

                  acaATTATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGGCGCTCAATATTTCGcaacaa                                                                                <  2:1749301/100‑1 (MQ=255)

                  acaATTATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGcaacaa                                                                                <  1:1878810/100‑1 (MQ=255)

                    aaTTATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGGCGCTCAATATTTCGCaacaaac                                                                              <  1:1231215/100‑1 (MQ=255)

                     aTTATGTTTTTCACATAATCAGTTCGACAATATGCGAGTGGTTCTGAAATATAACAATGAAAGCGATATAACGTCGGTGCTCAATAATGTGCaacaaac                                                                              >  1:652311/1‑99 (MQ=255)

                     aTTATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCaacaaaca                                                                             <  1:4014372/100‑1 (MQ=255)

                     aTTATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCaacaaaca                                                                             >  1:2216657/1‑100 (MQ=255)

                     aTTATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCaacaaaca                                                                             >  2:1567320/1‑100 (MQ=255)

                      ttATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACAt                                                                            <  1:1567316/100‑1 (MQ=255)

                       tATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGGCGCTCAATATTTCGCAACAAACAtt                                                                           >  2:1565009/1‑100 (MQ=255)

                       tATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACAtt                                                                           <  2:2137705/100‑1 (MQ=255)

                          gTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGGCGCTCAATATTTCGCAACAAACATTaaa                                                                        <  2:2089131/100‑1 (MQ=255)

                           tttttCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAg                                                                       >  1:1043663/1‑100 (MQ=255)

                            ttttCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGa                                                                      >  1:3831764/1‑100 (MQ=255)

                             tttCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGAt                                                                     >  2:229904/1‑100 (MQ=255)

                               tCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTc                                                                   <  2:514580/100‑1 (MQ=255)

                               tCACATAATCAGTTCAAAATTATGCAACTGAGGCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCGCAATATTTCGCAACAAACATTAAAGATTc                                                                   >  2:2994283/1‑100 (MQ=255)

                               tCACATAATCAATTAAAAATTATGCAACTAATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTc                                                                   <  1:3529316/100‑1 (MQ=255)

                                cacaTAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGATTCa                                                                  >  2:3261831/1‑100 (MQ=255)

                                cacaTAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGATTCa                                                                  >  1:982842/1‑100 (MQ=255)

                                cacaTAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGATTCa                                                                  <  2:4098338/100‑1 (MQ=255)

                                 acaTAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGATTCAg                                                                 <  1:674817/100‑1 (MQ=255)

                                 acaTAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAACGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCa                                                                  <  1:1620166/99‑1 (MQ=255)

                                    tAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGaaa                                                              >  2:1303127/1‑100 (MQ=255)

                                     aaTCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAAt                                                             <  1:1076366/100‑1 (MQ=255)

                                       tCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTc                                                           <  2:2257825/100‑1 (MQ=255)

                                       tCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTc                                                           <  1:3915984/100‑1 (MQ=255)

                                         aGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCaa                                                         <  1:3072584/100‑1 (MQ=255)

                                         aGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCaa                                                         <  1:1945078/100‑1 (MQ=255)

                                          gTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAAc                                                        >  2:3415260/1‑100 (MQ=255)

                                          gTTAAAAATTATGCAACTGACTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAAc                                                        <  1:4117658/100‑1 (MQ=255)

                                           ttCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACa                                                       <  2:3336667/100‑1 (MQ=255)

                                           ttCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACa                                                       >  2:3636148/1‑100 (MQ=255)

                                           ttCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACa                                                       <  2:33196/100‑1 (MQ=255)

                                            tCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACAt                                                      <  2:94649/100‑1 (MQ=255)

                                            tCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACAt                                                      >  1:3217224/1‑100 (MQ=255)

                                               aaaTTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTAt                                                   >  1:4198955/1‑100 (MQ=255)

                                                aaTTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATg                                                  <  1:1123126/100‑1 (MQ=255)

                                                    aTGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACa                                              >  1:160449/1‑100 (MQ=255)

                                                    aTGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACa                                              <  2:96524/100‑1 (MQ=255)

                                                    aTGCAACGGATTCAGAAAAATAAAAATGAAAGCAATAGCACGTCGGCACTCAATATTTCGCAACAAACATGAAAGATTCAGAAATTCAACATTATGTAc                                               <  1:1730608/99‑1 (MQ=255)

                                                       ccaCTGATTCTGAAAAATAAAAATGAAATCAATATCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAg                                            <  1:3497328/97‑1 (MQ=255)

                                                       cAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGc                                           <  2:2874060/100‑1 (MQ=255)

                                                        aaCTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCt                                          <  2:3921055/100‑1 (MQ=255)

                                                        aaCTGAATCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCt                                          <  2:2816644/100‑1 (MQ=255)

                                                          cTGCTTCTGAAAAACAAAAATGAAACCAATTTCACGTCGTCGCTCAATATTCCGCAACAAACAATAAAGATTCAGAAATTCAACATTATGTACATGCtaa                                        >  1:1519457/1‑98 (MQ=255)

                                                          cTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGTCGCTCAATATTTAGCAACAAAAATAAAAGATTAAGAAATTCAACATTATGTACaaacaga                                        >  1:464671/1‑95 (MQ=255)

                                                          cTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGa                                        <  1:28493/100‑1 (MQ=255)

                                                           tGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGaa                                       <  2:400997/100‑1 (MQ=255)

                                                           tGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGaa                                       >  1:1833181/1‑100 (MQ=255)

                                                           tGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGaa                                       <  1:3349210/100‑1 (MQ=255)

                                                             aTTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAAc                                     <  1:2548077/100‑1 (MQ=255)

                                                              ttCTGAAAAATAAAAATGAAAGCAATATCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACt                                    <  1:136695/100‑1 (MQ=255)

                                                                cTGAAAAATAAAAATGAAAGCAATATCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTaa                                  >  2:3100892/1‑100 (MQ=255)

                                                                cTGAAAAATAAAAATGAAAGCAATATCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTaa                                  >  1:3505429/1‑100 (MQ=255)

                                                                cTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTaa                                  >  2:3384244/1‑100 (MQ=255)

                                                                 tGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAg                                 >  1:452026/1‑100 (MQ=255)

                                                                  gAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGa                                <  2:4063558/100‑1 (MQ=255)

                                                                    aaaataaaaatGAAAGCAATATCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACg                              <  2:232421/100‑1 (MQ=255)

                                                                    aaaataaaaatGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACg                              <  2:427088/100‑1 (MQ=255)

                                                                    aaaataaaaatGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACg                              >  2:2078720/1‑100 (MQ=255)

                                                                       ataaaaatGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTAt                           >  2:124645/1‑100 (MQ=255)

                                                                         aaaaatGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGa                         >  1:1006225/1‑100 (MQ=255)

                                                                          aaaatGAAAGCAATATCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAg                        <  1:901319/100‑1 (MQ=255)

                                                                           aaatGAAAGCAATATCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGc                       <  2:3769985/100‑1 (MQ=255)

                                                                            aatGAAAGCAATATCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCg                      <  1:3415247/100‑1 (MQ=255)

                                                                            aatGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCg                      <  1:4198104/100‑1 (MQ=255)

                                                                            aatGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCg                      >  2:2260486/1‑100 (MQ=255)

                                                                             atGAAAGCAATATCACGTCGGCGCTCAATAGTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGa                     >  1:3137627/1‑100 (MQ=255)

                                                                              tGAAAGCAATATCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGAc                    <  2:1888693/100‑1 (MQ=255)

                                                                                aaaGCAATATCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACAt                  <  2:1443132/100‑1 (MQ=255)

                                                                                aaaGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACAt                  >  2:3596899/1‑100 (MQ=255)

                                                                                 aaGCAATATCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATc                 >  2:3189611/1‑100 (MQ=255)

                                                                                     aaTATCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCa             <  2:1021150/100‑1 (MQ=255)

                                                                                       tatCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCAcc           <  1:823296/100‑1 (MQ=255)

                                                                                        atCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCAccc          <  2:2705123/100‑1 (MQ=255)

                                                                                        atCACGTCGGCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCAccc          >  2:744977/1‑100 (MQ=255)

                                                                                         tCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCt         >  2:4214416/1‑100 (MQ=255)

                                                                                          cACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTg        >  2:1514914/1‑100 (MQ=255)

                                                                                          cACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTg        >  2:763570/1‑100 (MQ=255)

                                                                                             gTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGt     <  2:938408/100‑1 (MQ=255)

                                                                                               cGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTAt   >  1:496863/1‑100 (MQ=255)

CTTGCCCCATAAGAATTCACAATTATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAATAAAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATC  >  NC_000913/3658683‑3658878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A