Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F56 I0 R1	215	141.2	6785521	95.3%	6466601	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	281,581	G→A	64.3%	R135W (CGG→TGG)	yagA ←	CP4‑6 prophage; putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	281,581	0	G	A	64.3%	114.8 / 149.0	149	R135W (CGG→TGG)	yagA	CP4‑6 prophage; putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand): ref base G (23/30); new base A (44/52); total (67/82)
Fisher's exact test for biased strand distribution p-value = 8.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.25e-02

Only 100 of 150 total aligned reads displayed.

TCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTC  >  NC_000913/281484‑281680

 ccAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGcc                                                                                                  <  2:422392/100‑1 (MQ=255)

 ccAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGcc                                                                                                  <  2:1987710/100‑1 (MQ=255)

 ccAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGACCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGcc                                                                                                  >  2:3368318/1‑100 (MQ=255)

  cagcagGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGccc                                                                                                 >  1:2939121/1‑100 (MQ=255)

   agcagGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCg                                                                                                >  2:909196/1‑100 (MQ=255)

        ggTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCg                                                                                           >  2:919867/1‑100 (MQ=255)

        ggTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGCTCGAACCAGCCCGTGGCg                                                                                           <  1:49666/100‑1 (MQ=255)

          tGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCggg                                                                                         <  2:370234/100‑1 (MQ=255)

           gAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGa                                                                                        <  1:43424/100‑1 (MQ=255)

            aGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGaa                                                                                       >  2:2573948/1‑100 (MQ=255)

             gatcttGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCATGTTCGAACCAGCCCGTGGCGGGAAt                                                                                      <  2:2057576/95‑1 (MQ=255)

             gCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAAt                                                                                      >  1:1831173/1‑100 (MQ=255)

             gCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAAt                                                                                      >  1:1995278/1‑100 (MQ=255)

              cGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATg                                                                                     <  1:598273/100‑1 (MQ=255)

               gaaTGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGc                                                                                    >  2:2285232/3‑100 (MQ=255)

                  tGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAAtagatc                                                                                 >  1:2057563/1‑95 (MQ=255)

                  tGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCg                                                                                 <  1:2352154/100‑1 (MQ=255)

                    gCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGt                                                                               >  1:2982529/1‑100 (MQ=255)

                    gCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGt                                                                               >  1:1434166/1‑100 (MQ=255)

                    gCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGt                                                                               >  2:313743/1‑100 (MQ=255)

                     cAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTg                                                                              <  2:1221059/100‑1 (MQ=255)

                     cAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTg                                                                              >  2:2318043/1‑100 (MQ=255)

                      aGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGCATGCCCGGTg                                                                              >  1:1519132/1‑99 (MQ=255)

                      aGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACAAGCCCGTGGCGGGAATGCCCGGTg                                                                              >  1:2703840/1‑99 (MQ=255)

                         ggccACCGCCAAAAGGAAAGTGGCCCGTAAAACCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAg                                                                           <  2:2827648/97‑1 (MQ=255)

                         gTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCCTCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCACCCCGTGCCGGGCATGCCCGCCGAAgc                                                                          >  1:2721365/1‑100 (MQ=255)

                           ccACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAgcgc                                                                        >  2:2700192/1‑100 (MQ=255)

                            cACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGcc                                                                       >  1:3272661/1‑100 (MQ=255)

                            cACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGcc                                                                       >  2:1736127/1‑100 (MQ=255)

                             aCCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAgcgc                                                                        >  1:223069/1‑98 (MQ=255)

                             aCCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGccc                                                                      >  2:649175/1‑100 (MQ=255)

                              ccGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCg                                                                     >  1:658896/1‑100 (MQ=255)

                              ccGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCg                                                                     >  1:1111539/1‑100 (MQ=255)

                              ccGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCg                                                                     <  2:1753623/100‑1 (MQ=255)

                                gCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGgc                                                                   <  2:948206/100‑1 (MQ=255)

                                 ccAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGgca                                                                  <  2:1347108/100‑1 (MQ=255)

                                     aaGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGcagcag                                                              <  1:234934/100‑1 (MQ=255)

                                      aGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCGCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAgg                                                             <  1:1803383/100‑1 (MQ=255)

                                       ggAGAGTGGCCCTTAAAATCCAGCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGc                                                            <  1:2901030/100‑1 (MQ=255)

                                              ggCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGc                                                      <  1:1218282/99‑1 (MQ=255)

                                               gCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCgg                                                    >  2:2928209/1‑100 (MQ=255)

                                                gccTTAAAATCCATCTGCGAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCGCGGTGAAGCGCCCGGCAGCAGGCCATGGCagg                                                   <  1:288295/99‑1 (MQ=255)

                                                 ccTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGc                                                  <  1:538403/100‑1 (MQ=255)

                                                 ccTTAAAAGCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCgggn                                                  <  1:2130874/100‑2 (MQ=255)

                                                  cTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGcc                                                 <  1:29411/100‑1 (MQ=255)

                                                  cTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGcc                                                 >  2:1198819/1‑100 (MQ=255)

                                                   ttAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCa                                                <  1:1121708/100‑1 (MQ=255)

                                                    tAAAATCCATCTACCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCa                                                >  2:2230151/1‑99 (MQ=255)

                                                      aaaTCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCa                                             <  2:2569236/100‑1 (MQ=255)

                                                       aaTCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAg                                            <  2:1300281/100‑1 (MQ=255)

                                                       aaTCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAg                                            <  2:1626022/100‑1 (MQ=255)

                                                        tccgaTCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAgg                                           <  1:1705003/96‑1 (MQ=255)

                                                        aTCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAgg                                           <  2:7430/100‑1 (MQ=255)

                                                          ccATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGtt                                         <  1:3175557/100‑1 (MQ=255)

                                                           cATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTa                                        >  1:136377/1‑100 (MQ=255)

                                                            aTCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTAt                                       <  2:2467867/100‑1 (MQ=255)

                                                             tCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATg                                      <  1:1498704/100‑1 (MQ=255)

                                                             tCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATg                                      <  2:1234804/100‑1 (MQ=255)

                                                              cTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATgg                                     >  1:3067563/1‑100 (MQ=255)

                                                                gCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACGAGCCCGTGGCGGGAAGGGCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGAc                                   <  1:562232/100‑1 (MQ=255)

                                                                gCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGAc                                   >  1:3136029/1‑100 (MQ=255)

                                                                gCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGAc                                   >  1:2441243/1‑100 (MQ=255)

                                                                 ccAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACg                                  >  2:1895830/1‑100 (MQ=255)

                                                                 ccAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACg                                  >  2:770572/1‑100 (MQ=255)

                                                                  cAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACgg                                 <  2:1301968/100‑1 (MQ=255)

                                                                     agGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGc                              <  2:1562527/100‑1 (MQ=255)

                                                                     agGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGc                              <  2:796450/100‑1 (MQ=255)

                                                                      ggCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCt                             >  2:2128304/1‑100 (MQ=255)

                                                                      ggCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGACATCAGGTTATGGACGGTGc                              <  1:3047431/99‑1 (MQ=255)

                                                                      ggCGGTTCGGCGCGGCGGGTCCGAACCAGCCCGGGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCAATCAGGTGAGGGACGGTGCt                             <  1:2907485/100‑1 (MQ=255)

                                                                        cGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGa                           <  1:2285216/100‑1 (MQ=255)

                                                                        cGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGa                           <  1:1626313/100‑1 (MQ=255)

                                                                          gTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAGGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAg                         <  1:2573928/100‑1 (MQ=255)

                                                                           ttCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAgg                        <  2:1245539/100‑1 (MQ=255)

                                                                             cGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGc                       <  1:509982/99‑1 (MQ=255)

                                                                              ggCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCgg                     >  2:906652/1‑100 (MQ=255)

                                                                              ggCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCg                      >  1:2506635/1‑99 (MQ=255)

                                                                                cgcgTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGc                   <  2:3095941/100‑1 (MQ=255)

                                                                                   gtcgtGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATg                <  2:1893732/100‑1 (MQ=255)

                                                                                    tcgtGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATgg               <  1:2916023/100‑1 (MQ=255)

                                                                                    tcgtGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATgg               <  1:2553067/100‑1 (MQ=255)

                                                                                     cgtGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGgt              <  1:2044869/100‑1 (MQ=255)

                                                                                      gtgtTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGgtg             <  2:2652538/100‑1 (MQ=255)

                                                                                      gtgtTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGgtg             <  2:2049110/100‑1 (MQ=255)

                                                                                       tgtTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGgtgt            >  2:1146691/1‑100 (MQ=255)

                                                                                        gtTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGtgtg           <  1:2461060/100‑1 (MQ=255)

                                                                                         ttCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGc          >  2:1108178/1‑100 (MQ=255)

                                                                                          tCGAACCAGCCCGTGGCGGGAATGCCCGGTGACGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGCTATGGACGGTGCTGAAGGCGCGCATgg               >  1:880998/1‑94 (MQ=255)

                                                                                          tCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGcc         <  2:523362/100‑1 (MQ=255)

                                                                                          tCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGcc         >  2:1377867/1‑100 (MQ=255)

                                                                                          tCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGcc         <  2:2304773/100‑1 (MQ=255)

                                                                                           cGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGccc        >  2:3246297/1‑100 (MQ=255)

                                                                                           cGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGccc        >  2:2172341/1‑100 (MQ=255)

                                                                                           cGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGccc        >  1:583045/1‑100 (MQ=255)

                                                                                           cGAACCAGCCCGTGGCGGGAATACCCGGTGAGACGCCCGGCAGCAGGCCATAGCGGGCCAGCAGGCTATGGACAGTGCTGAAGGCGGGCATGgtgt            >  1:1517760/1‑96 (MQ=255)

                                                                                            gAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGcccc       >  2:305955/1‑100 (MQ=255)

                                                                                             aaCCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCt      <  1:2973676/100‑1 (MQ=255)

                                                                                              aCCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTg     <  2:2520853/100‑1 (MQ=255)

                                                                                               ccGGCCCGTGGCGGGGATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGGTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCt      <  1:2526707/98‑1 (MQ=255)

                                                                                                cGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGt   <  2:2005221/100‑1 (MQ=255)

TCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTC  >  NC_000913/281484‑281680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A