Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F212 I0 R1	246	122.0	6097616	93.1%	5676880	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	281,581	G→A	100%	R135W (CGG→TGG)	yagA ←	CP4‑6 prophage; putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	281,581	0	G	A	99.3%	483.0 / ‑6.0	146	R135W (CGG→TGG)	yagA	CP4‑6 prophage; putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (69/75); total (70/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

TCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCT  >  NC_000913/281484‑281676

 ccAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGc                                                                                               <  2:1529468/99‑1 (MQ=255)

  cagcagGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGccc                                                                                             >  2:1232689/1‑100 (MQ=255)

     cagGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTg                                                                                          <  1:2091123/100‑1 (MQ=255)

      agGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAATGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTgg                                                                                         <  1:74775/100‑1 (MQ=255)

      agGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTgg                                                                                         >  2:1826753/1‑100 (MQ=255)

       gggTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGc                                                                                        <  1:1273374/100‑1 (MQ=255)

       gggTGAGCGGATGACAGCGTCCACCGCCAAAAGGAAAGGGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGc                                                                                        >  2:1876187/1‑100 (MQ=255)

        ggTGAGCGGACGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCg                                                                                       <  2:1997724/100‑1 (MQ=255)

         gTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTACCAGAGACGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCgg                                                                                      >  1:74298/1‑100 (MQ=255)

          tgggCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCggg                                                                                     <  2:2301404/97‑1 (MQ=255)

          tGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCggg                                                                                     <  2:841951/100‑1 (MQ=255)

          tGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCggg                                                                                     <  1:1842514/100‑1 (MQ=255)

           gAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGa                                                                                    <  1:2221612/100‑1 (MQ=255)

             gCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGaa                                                                                   <  2:841596/99‑1 (MQ=255)

                  tGGCAGCGTCCACCGTCAAAAGGAAAGTTGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGccc                                                                              <  2:371380/99‑1 (MQ=255)

                  tGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCg                                                                             <  2:326518/100‑1 (MQ=255)

                    gCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGt                                                                           >  2:2607328/1‑100 (MQ=255)

                    gCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGt                                                                           >  1:1959167/1‑100 (MQ=255)

                    gCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGt                                                                           >  1:2768546/1‑100 (MQ=255)

                      aGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGa                                                                         >  2:1007340/1‑100 (MQ=255)

                      aGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGa                                                                         <  1:984040/100‑1 (MQ=255)

                      aGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGa                                                                         >  1:1343234/1‑100 (MQ=255)

                        cGTCCACCGCCAAAAGGAAAGTGGCTCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAg                                                                       <  1:977795/100‑1 (MQ=255)

                        cGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAg                                                                       <  2:1968681/100‑1 (MQ=255)

                         gTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAg                                                                       <  1:2640186/99‑1 (MQ=255)

                           ccgatctcAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAgcgc                                                                    <  2:1153326/93‑1 (MQ=255)

                            cACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGcc                                                                   >  2:1123963/1‑100 (MQ=255)

                               cGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCgg                                                                >  2:2069949/1‑100 (MQ=255)

                               cGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCgg                                                                <  1:515817/100‑1 (MQ=255)

                                gCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGgc                                                               <  1:2173075/100‑1 (MQ=255)

                                gCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGgc                                                               <  1:1013174/100‑1 (MQ=255)

                                gCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGgc                                                               >  2:1623928/1‑100 (MQ=255)

                                 ccACAAGCAAAGTGTCCCTTACAATCCGTCCGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGgca                                                              <  2:1320871/100‑1 (MQ=255)

                                 ccAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGgc                                                               >  2:1024084/1‑99 (MQ=255)

                                  cAAGAGGGAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCTACGCGTAGTGTTCGACCCGGCCCGTGACGGGAATGCCAGTTGAAGCGCCCAgca                                                              >  2:2467475/1‑99 (MQ=255)

                                  cAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGgcag                                                             >  1:314629/1‑100 (MQ=255)

                                  cAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGgcag                                                             >  2:2060926/1‑100 (MQ=255)

                                  cAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCCGGAATGCCCGGTGAAGCGCCCGgca                                                              >  2:2321367/1‑99 (MQ=255)

                                     aaGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGcagcag                                                          <  1:60553/100‑1 (MQ=255)

                                         aaaGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAGCCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCa                                                      <  2:766250/100‑1 (MQ=255)

                                          aaGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCAt                                                     >  2:1894710/1‑100 (MQ=255)

                                            gTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATgg                                                   >  1:139361/1‑100 (MQ=255)

                                               gCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCg                                                 >  2:2898686/1‑99 (MQ=255)

                                                cccTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCggg                                               <  1:2718215/100‑1 (MQ=255)

                                                cccTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCggg                                               >  2:2207607/1‑100 (MQ=255)

                                                cccTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCggg                                               <  2:2436559/100‑1 (MQ=255)

                                                 ccTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGATCCAGCCCGTGGCGGGAATGCCCGGTGAAGGGCCCGGCAGCAGGCCATGGCGGGc                                              <  1:616280/100‑1 (MQ=255)

                                                 ccTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAAGCAGCCCGTGGCGGGAATGCGCGGTGAAGCGCCCGGCAGCAGGCCATGGCgggg                                              <  2:76120/100‑2 (MQ=255)

                                                 ccTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGc                                              >  1:1696200/1‑100 (MQ=255)

                                                  cTTAAAATCCATCTGCCAGAGGCGGTTCGGCGTGTCGTGTTCGAACCAGCCCGTAGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGcc                                             <  1:1745514/100‑1 (MQ=255)

                                                  cTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGcc                                             <  2:2033257/100‑1 (MQ=255)

                                                  cTTAAAACCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGcc                                             <  2:54294/100‑1 (MQ=255)

                                                   ttAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGc                                              <  1:2129448/98‑1 (MQ=255)

                                                    naaaaCCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAACGCCCGGCAGCAGGCCATGGCGGGCCAt                                           >  1:1139625/2‑100 (MQ=255)

                                                        aTCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAgg                                       >  1:1448399/1‑100 (MQ=255)

                                                         tCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCTCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGt                                      <  1:81204/100‑1 (MQ=255)

                                                          ccATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAgg                                       >  1:1368282/1‑98 (MQ=255)

                                                           cATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTa                                    >  1:2233382/1‑100 (MQ=255)

                                                           cATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTa                                    >  2:11546/1‑100 (MQ=255)

                                                            aTCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTAt                                   >  2:1060977/1‑100 (MQ=255)

                                                             tCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATg                                  <  1:1337875/100‑1 (MQ=255)

                                                              cTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATgg                                 >  1:341515/1‑100 (MQ=255)

                                                              cTGCCAGAGGCGGTTCGGCGCGACGTGTTGGAACCAGACCGGGGCAGGAATGCCCGGTGAAGCGCCAGGGAGAAGGCCATGGCGGGCCACCAGGTTATg                                  >  2:2466538/1‑99 (MQ=255)

                                                               tGCCAGAGGCGGTTCGGCGCGTCGAGTACGAACCAGCCCGGGTCGGGAATGCTCGGTGAGGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTAAGGa                                >  2:699197/1‑100 (MQ=255)

                                                                     agGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGc                          <  2:2099807/100‑1 (MQ=255)

                                                                      ggCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCt                         >  2:525491/1‑100 (MQ=255)

                                                                      ggCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCt                         >  2:657236/1‑100 (MQ=255)

                                                                      ggCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCt                         >  1:837395/1‑100 (MQ=255)

                                                                       gCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTg                        <  2:2428281/100‑1 (MQ=255)

                                                                         ggTTCGGCGCGTCGTTTTCGAACCAGCCGGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGaa                      <  1:2126790/100‑1 (MQ=255)

                                                                          gTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGATATGAACGGTGCTGAAg                     >  1:521361/1‑100 (MQ=255)

                                                                          atctggCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAg                     <  1:2474390/96‑1 (MQ=255)

                                                                           ttCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAgg                    <  2:1091990/100‑1 (MQ=255)

                                                                           ttCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGACAACAGGCCATGACGGACCATCAGGTTATGGAAGGTGCTGAAg                     >  1:1112977/1‑99 (MQ=255)

                                                                              ggCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCCGGTTATGGACGGTGCTGAAGGCgg                 >  2:2497568/1‑100 (MQ=255)

                                                                              ggCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCgg                 >  2:225805/1‑100 (MQ=255)

                                                                              ggCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCgg                 >  1:1926764/1‑100 (MQ=255)

                                                                              ggCGCGTCGTGTTCGAACCAGCACGAGGCGGGAATGCCCGGTGAATCGCCCGGCAGCAGGCCATGGCGGGCCATCCGGTTATGGACGGTGCTGAAGGCg                  >  2:1123932/1‑99 (MQ=255)

                                                                               gcgcGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGTTGCTGAAGGCggg                <  1:2041009/100‑1 (MQ=255)

                                                                               gcgcGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCggg                >  1:2660845/1‑100 (MQ=255)

                                                                                cgcgTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGc               <  2:1696180/100‑1 (MQ=255)

                                                                                cgcgTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGc               >  1:2326901/1‑100 (MQ=255)

                                                                                 gcgTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCa              <  1:2247222/100‑1 (MQ=255)

                                                                                 gcgTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCa              <  1:2328325/100‑1 (MQ=255)

                                                                                   gtcgtGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCGCGGCAGCAGGCCATGGTGGGCCATCAGGTTATGGACGGTGCTGAAGGc                   <  2:457680/93‑1 (MQ=255)

                                                                                    tcgtGTTCGAATCAGTTTGTGGCGTTAATGCCCGGTGAAGCGCTCGGCAGCAGGCCATGGCGGGCAATCAGGTTATGGACGGTGCTGAAGGCGGGCATgg           <  1:1141240/100‑1 (MQ=255)

                                                                                    tcgtGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATgg           >  1:2237683/1‑100 (MQ=255)

                                                                                     cgtGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGgt          <  1:11546/100‑1 (MQ=255)

                                                                                      gtgtTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGgtg         <  2:2717821/100‑1 (MQ=255)

                                                                                      gtgtTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGgtg         <  1:978238/100‑1 (MQ=255)

                                                                                       tgtTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGgtgt        >  1:487683/1‑100 (MQ=255)

                                                                                        gtTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGtgtg       >  1:439804/1‑100 (MQ=255)

                                                                                          tCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGcc     >  1:1068197/1‑100 (MQ=255)

                                                                                          tCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGcc     <  2:2942330/100‑1 (MQ=255)

                                                                                          tCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGcc     >  1:2942362/1‑100 (MQ=255)

                                                                                            gAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGcccc   <  2:1221312/100‑1 (MQ=255)

                                                                                            gAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGcccc   >  2:2824711/1‑100 (MQ=255)

                                                                                            gAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGcccc   <  2:2237660/100‑1 (MQ=255)

                                                                                             aaCCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCt  <  1:1887477/100‑1 (MQ=255)

                                                                                             aaCCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCt  <  1:2824743/100‑1 (MQ=255)

TCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCT  >  NC_000913/281484‑281676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A