Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F56 I1 R1
|
155 |
65.2 |
3164312 |
95.9% |
3034575 |
99.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,452,057 |
A→T |
I77N (ATC→AAC) |
rplD ← |
50S ribosomal subunit protein L4 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,452,057 | 0 | A | T | 95.8%
| 231.5
/ ‑0.0
| 71 | I77N (ATC→AAC) | rplD | 50S ribosomal subunit protein L4 |
| Reads supporting (aligned to +/- strand): ref base A (0/3); new base T (35/33); total (35/36) |
| Fisher's exact test for biased strand distribution p-value = 2.39e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.36e-01 |
CAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGC > NC_000913/3451960‑3452154
|
cAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTc > 2:323952/1‑100 (MQ=255)
ggATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCgg > 1:240068/1‑100 (MQ=255)
ggATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCgg > 2:1190267/1‑100 (MQ=255)
ggATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACATTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCg > 1:498782/1‑99 (MQ=255)
gATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCggg > 2:316738/1‑100 (MQ=255)
gCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGctc > 2:1181340/1‑100 (MQ=255)
ttttCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCtt > 2:1379611/1‑100 (MQ=255)
tttCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTg < 1:354227/100‑1 (MQ=255)
tCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGAt < 1:1181057/100‑1 (MQ=255)
cAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATa < 1:1225070/100‑1 (MQ=255)
cAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTGGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATa < 2:941137/100‑1 (MQ=255)
aGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAg < 1:350258/100‑1 (MQ=255)
aGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAg > 1:760475/1‑100 (MQ=255)
gcgcgcCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGa < 2:285063/100‑1 (MQ=255)
gccgcGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCa < 1:1350110/100‑1 (MQ=255)
gccgcGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCa < 2:537096/100‑1 (MQ=255)
cgcgGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGa < 2:1173759/100‑1 (MQ=255)
cgGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAGAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAAc < 2:1472985/100‑1 (MQ=255)
tACATCTTCTTGTTAACTTTTTTTTTTTTTTTTTGAGGACGAGCAGCAAAGGTTACTCCACTTTTTCGCCAGTTCGGGCTCTTGATATTTCCAGAActct > 2:626983/1‑97 (MQ=255)
aCATCTTCTTGTTAACTTTTTTACTTTGTTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACgcgc > 2:847051/1‑100 (MQ=255)
aCATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACgcgc > 1:1360504/1‑100 (MQ=255)
cttcttGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACgg > 1:1093579/1‑100 (MQ=255)
cttcttGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACgg > 2:1576950/1‑100 (MQ=255)
ctgcttGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAGCGCCAGTTCAGGCTCTTGATAGAACCAGAAcg < 2:555788/90‑1 (MQ=255)
tcttGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGcc > 2:819463/1‑100 (MQ=255)
cttGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCg > 2:448747/1‑100 (MQ=255)
gTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTg < 1:699894/100‑1 (MQ=255)
tAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGcc < 1:281572/100‑1 (MQ=255)
aaCTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCt > 2:268828/1‑100 (MQ=255)
cTTTTTGACTGTGGTCCTGCGGACGAGCGCCAACGGTCACGCCACCAGAACGCCAGTCCGGCCTCTTGATAGAACCATAACGCGCACGGCCAGTGccct > 2:10854/1‑97 (MQ=255)
ttttGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCt < 2:813529/100‑1 (MQ=255)
tttGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTg < 1:465100/100‑1 (MQ=255)
tttGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTg < 1:464510/100‑1 (MQ=255)
ttGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTGGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTgg > 2:293236/1‑100 (MQ=255)
tGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGgc < 1:334905/100‑1 (MQ=255)
tGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGgc < 1:756521/100‑1 (MQ=255)
tGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTCCTGgc > 1:574420/1‑100 (MQ=255)
aCTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGTCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGgcgc < 2:27627/100‑1 (MQ=255)
cTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGcc > 1:199219/1‑100 (MQ=255)
gtgGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTACCTTTCTGGCGCCAc > 1:77933/1‑100 (MQ=255)
gTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGt > 1:210841/1‑100 (MQ=255)
cTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGtttt < 1:208398/100‑1 (MQ=255)
tGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGttttt < 2:16265/100‑1 (MQ=255)
gCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGtttttt < 2:230677/100‑1 (MQ=255)
ggACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTAc < 2:1216270/100‑1 (MQ=255)
gACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTAcc < 1:268828/100‑1 (MQ=255)
cGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCgg < 2:1163834/100‑1 (MQ=255)
gcagcaAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAAc > 1:1157237/1‑100 (MQ=255)
cagcaAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAAcc < 1:1576960/100‑1 (MQ=255)
agcaAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCa > 2:1456414/1‑100 (MQ=255)
agcaAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCa > 2:459651/1‑100 (MQ=255)
agcaAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCa > 1:1334760/1‑100 (MQ=255)
gcaATGGCCCCGCCACCAAAACGACAGTTCGGGCACTTGAGATAACCAAAACGCGCACGGCCGGGGCCTTTCTGGCGCCACGCGTTTTTACCgg > 2:887581/1‑94 (MQ=255)
gcaAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAg > 1:632121/1‑100 (MQ=255)
aaaGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGtt > 2:1117043/1‑100 (MQ=255)
aaaGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGtt < 2:515877/100‑1 (MQ=255)
aGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTAc > 1:961950/1‑100 (MQ=255)
aGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTAc < 1:485302/100‑1 (MQ=255)
ggTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACt < 2:186674/100‑1 (MQ=255)
gTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACtt > 1:814828/1‑100 (MQ=255)
tCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTc < 1:44554/100‑1 (MQ=255)
cACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCa > 1:551138/1‑100 (MQ=255)
cGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGc < 2:576691/100‑1 (MQ=255)
gCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCa > 2:164941/1‑100 (MQ=255)
ccaccaGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCAc < 1:659846/100‑1 (MQ=255)
caccaGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACg < 1:478069/100‑1 (MQ=255)
ccaGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAg < 2:509349/100‑1 (MQ=255)
aCGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtcttc > 2:83791/1‑100 (MQ=255)
aCGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtcttc < 1:1330678/100‑1 (MQ=255)
cGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtcttct > 2:735936/1‑100 (MQ=255)
aGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGc < 2:928497/100‑1 (MQ=255)
|
CAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGC > NC_000913/3451960‑3452154
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
