Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F142 I1 R1
|
99 |
64.8 |
3148104 |
96.3% |
3031624 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,881,837 |
G→T |
T162N (ACC→AAC) |
dnaN ← |
DNA polymerase III, beta subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,881,837 | 0 | G | T | 95.5%
| 221.3
/ ‑0.0
| 67 | T162N (ACC→AAC) | dnaN | DNA polymerase III, beta subunit |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base T (26/38); total (27/40) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCG > NC_000913/3881741‑3881930
|
aTCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGtttt < 2:469707/100‑1 (MQ=255)
aTCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGtttt < 1:654488/100‑1 (MQ=255)
aTCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGtttt > 1:1112417/1‑100 (MQ=255)
tCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTc < 2:1544424/100‑1 (MQ=255)
tCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTc < 2:1383801/100‑1 (MQ=255)
cACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGttttga > 2:735954/1‑98 (MQ=255)
cACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCa > 1:1422900/1‑100 (MQ=255)
cACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCa > 2:1419545/1‑100 (MQ=255)
cACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCa > 2:862889/1‑100 (MQ=255)
aCCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCaa < 2:31298/100‑1 (MQ=255)
ccGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCTGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCan < 2:1197267/99‑2 (MQ=255)
cGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAAc < 1:1176179/100‑1 (MQ=255)
aTGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGc > 2:725202/1‑100 (MQ=255)
gCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATa > 1:1503897/1‑100 (MQ=255)
gCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATa > 2:782394/1‑100 (MQ=255)
gCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATa > 1:690731/1‑100 (MQ=255)
tGGCAAAGCTTGACCAAGTGGCATTGAACAGACCGCCAAACGGTGGCCGTCGGTTGCAACGGTGCGCAGTTCTTGACCTTCGTTTTCAAACAGCATACCa > 2:1454078/1‑100 (MQ=255)
ggCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCAt > 1:840731/1‑100 (MQ=255)
ggCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCAt > 2:1349503/1‑100 (MQ=255)
aaaGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTa < 2:753084/100‑1 (MQ=255)
aGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAg < 1:728119/100‑1 (MQ=255)
aGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAg < 1:246864/100‑1 (MQ=255)
aGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAg < 2:789293/100‑1 (MQ=255)
gATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGt < 1:20038/100‑1 (MQ=255)
aTTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTa < 1:862896/100‑1 (MQ=255)
ttGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTaa > 2:71814/1‑100 (MQ=255)
ttGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTaa > 2:1413144/1‑100 (MQ=255)
ttGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTaa < 2:582811/100‑1 (MQ=255)
gACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATa > 2:1229404/1‑100 (MQ=255)
gACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATa < 1:1262615/100‑1 (MQ=255)
ctccAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATa < 1:1250347/98‑1 (MQ=255)
aCCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAg < 2:496145/100‑1 (MQ=255)
ccAATTGGCATTGAACAGACCGCCCGACTGTGGCCGTCCGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAg < 2:497074/99‑1 (MQ=255)
ccAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATaag > 2:1250339/1‑98 (MQ=255)
ccAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGc > 1:105704/1‑100 (MQ=255)
cAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCg < 2:1218343/100‑1 (MQ=255)
cAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCg < 2:1422890/100‑1 (MQ=255)
aTTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGaa < 1:716409/100‑1 (MQ=255)
ggCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGt > 1:1261822/1‑100 (MQ=255)
aTTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCt < 1:1014878/100‑1 (MQ=255)
aTTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCt < 1:1411529/100‑1 (MQ=255)
ttGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCt < 1:1438482/99‑1 (MQ=255)
tGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGa > 1:1002433/1‑100 (MQ=255)
tGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGa > 2:421954/1‑100 (MQ=255)
gAACAGGCCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGAt < 1:317220/100‑1 (MQ=255)
gAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGAt > 2:1051203/1‑100 (MQ=255)
aCAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATgc > 2:1481499/1‑100 (MQ=255)
gACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGcc < 1:196070/100‑1 (MQ=255)
ccGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCAt > 2:1013870/1‑100 (MQ=255)
tGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCTTAGAAAACTggg < 1:1030776/100‑1 (MQ=255)
tGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTggg < 2:1384609/100‑1 (MQ=255)
tGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAACTAATAGCGAACGTCCTGATGCGCCATAGAAAACTggg < 2:841096/100‑1 (MQ=255)
ggCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGt < 2:558156/100‑1 (MQ=255)
cGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGc > 2:770490/1‑100 (MQ=255)
tCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCtt < 1:1103661/100‑1 (MQ=255)
tCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCtt < 1:707714/100‑1 (MQ=255)
cGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTc < 2:105704/100‑1 (MQ=255)
ggTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCa < 1:554994/100‑1 (MQ=255)
ccACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGAGCCATAGAAAACTGGGTCGCTTCAATc > 2:944011/1‑98 (MQ=255)
ggTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGc < 1:1127622/100‑1 (MQ=255)
gggcAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTc < 1:680877/98‑1 (MQ=255)
gcgcAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTc < 2:1261813/100‑1 (MQ=255)
gcAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCAt < 1:71814/100‑1 (MQ=255)
cAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATc < 2:161918/100‑1 (MQ=255)
aGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCg < 1:942277/100‑1 (MQ=255)
ttcttcACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGtt < 2:418814/100‑1 (MQ=255)
cttcACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGc > 1:287586/1‑100 (MQ=255)
ttcACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTg > 2:1442304/1‑98 (MQ=255)
cACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTg > 1:754893/1‑100 (MQ=255)
ccTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCg > 1:463319/1‑100 (MQ=255)
ccTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCg < 2:809065/100‑1 (MQ=255)
|
ATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCG > NC_000913/3881741‑3881930
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
