Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F256 I0 R1
|
328 |
102.7 |
5123138 |
93.4% |
4785010 |
99.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
3,946,246 |
G→A |
20.5% |
noncoding (2543/2904 nt) |
rrlC → |
23S ribosomal RNA of rrnC operon |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,946,246 | 0 | G | A | 20.5%
| 110.7
/ 21.9
| 64 | noncoding (2543/2904 nt) | rrlC | 23S ribosomal RNA of rrnC operon |
| Reads supporting (aligned to +/- strand): ref base G (23/27); new base A (3/10); total (26/38) |
| Fisher's exact test for biased strand distribution p-value = 2.07e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01 |
GGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGG > NC_000913/3946147‑3946327
|
ggggATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAg < 1:990940/100‑1 (MQ=33)
ggggATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAg < 1:1080880/100‑1 (MQ=33)
ggggATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCAAAg < 2:117032/100‑1 (MQ=17)
gggATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAgg < 1:1947522/100‑1 (MQ=33)
ggATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAggg < 1:1150919/100‑1 (MQ=33)
ggATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAggg < 1:1450221/100‑1 (MQ=32)
ggATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAggg < 1:2049307/100‑1 (MQ=33)
aaCAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATg < 1:628738/100‑1 (MQ=33)
aaCAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGAACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATg < 1:210847/100‑1 (MQ=18)
agaGGCTGATACCACCCAAGAGTTCATATCGACGGCGGTGTTAGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATgg > 1:1734372/3‑100 (MQ=255)
aCAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATgg > 1:1065859/1‑100 (MQ=32)
cAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGc < 2:1773006/100‑1 (MQ=33)
cAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAAGGTATGGc < 2:209108/100‑1 (MQ=18)
ggCTGATACCGCCCAAGCGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCCCATCCAGGAGCTGACGTAGGTCCCAAGGGCATGGCTg > 2:1725342/1‑100 (MQ=11)
cTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGtt > 1:2264490/1‑100 (MQ=32)
gATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAAGGTATGGCTGTTCg < 2:1261078/100‑1 (MQ=18)
ccGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAAGGTATGGCTGTTCGCCAt < 2:2065723/100‑1 (MQ=18)
cGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCAtt > 1:259507/1‑100 (MQ=32)
cccAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTa > 1:165087/1‑100 (MQ=32)
cccAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTa < 1:1079606/100‑1 (MQ=33)
cccAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAAGGTATGGCTGTTCGCCATTTa > 1:1778074/1‑100 (MQ=17)
aaGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAg < 2:2478597/100‑1 (MQ=18)
aaGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAg < 2:179462/100‑1 (MQ=17)
agagTTCATCTCGCCGGCGGTGTTTGGCACATCGATGTAGGCTCCTTACCTCCTGGAGATGACGTCGGTCCCCACGGTATGGCTGTTCGCAATTTGAAg < 2:2137361/99‑1 (MQ=2)
agagTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGTAGTAGGTCCCAAGGGTATGGCTGTTGGCCATTTAAAGt > 2:890217/1‑100 (MQ=12)
gagTTCATATCGACGGCGGTGTTTGGTACCTCGATGTCGGTTCATCACATCCAGGAGCTGAAGTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTg < 2:260267/100‑1 (MQ=11)
agTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTgg < 2:1930050/100‑1 (MQ=33)
agTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTg < 2:2048431/99‑1 (MQ=17)
gTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTGGCCATTTAAAGTGGt > 2:1211440/1‑100 (MQ=17)
ttCGTATCGACGGCGGTGTTTGGCACCTCGCTGTAGTCTTATCAAATCCTATAGCTGAAGTAAGTCCCAAATTTATTACTGTTCTCCATTTAAAg < 2:2393713/95‑1 (MQ=2)
cATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTAcg < 2:945537/100‑1 (MQ=32)
cATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTAcg > 1:2000158/1‑100 (MQ=33)
tCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAg < 2:178046/100‑1 (MQ=33)
cGACGGCGGTGTTTGGCACCTCGATGTCGGATCATCACATCCTGAAGCTGAAATAGGTCCCAAAGGTATGGATGTTCGCCATTTAAAGTGGTACGCGa > 2:1453665/1‑98 (MQ=11)
gACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCt > 1:1770588/1‑100 (MQ=33)
cggcggTGTTTGGCACCTCGATGTCGTCTCATCACATCCTGGAGCTGAAGTAGTTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTAcg > 2:1986064/1‑92 (MQ=12)
gcggTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGt > 1:1767233/1‑100 (MQ=33)
cggTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGtt < 1:2371909/100‑1 (MQ=32)
cggTGTTTGGCACATCGATGTAGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGtt > 2:2546528/1‑100 (MQ=12)
ggTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGttt < 2:2528417/100‑1 (MQ=33)
ggTGTTGGGTACCTCGTTGTCGGCTCATCACATCCTGGAGCTGAAGTGGGTCCCAAGGGTATGGCTGTTCGCCTTTTAAAGTGGTACGCGAGCTGGGttt < 2:1620853/100‑1 (MQ=11)
gtgtTTGGCACCTCGATGTCGGCTCAGCACATCCTGGAGCTGAAGTAGGTACCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTa < 2:1728832/100‑1 (MQ=12)
gtTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAg > 1:1649960/1‑99 (MQ=32)
gtTTGGCACCTCGATGTCCGATCATCACATCCTGTAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATGTAAAGTGGTACGCGAGCTGGGTTTAGa < 2:1756771/100‑1 (MQ=11)
ttGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAAc < 1:1924075/100‑1 (MQ=33)
ttGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAAc > 1:115267/1‑100 (MQ=32)
naCCTCAATGTCGACTCATCACATACGGAATCTGAAGTAGGTCCCAAGGGTATTGTTGTTCGCCATATAAAGTGGTACGCGAACGGGCTTTAGAAcatc > 2:1124309/2‑99 (MQ=2)
cACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAAcgtcg < 1:1619354/100‑1 (MQ=32)
cACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAAcgtcg < 1:1991324/100‑1 (MQ=33)
cACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAAcgtcg > 2:306959/1‑100 (MQ=32)
cACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAAcgtcg < 2:1778061/100‑1 (MQ=17)
cTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTga < 2:1792072/100‑1 (MQ=32)
tCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTgag < 1:1124983/100‑1 (MQ=33)
tCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTgag < 2:2438977/100‑1 (MQ=33)
cGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTgaga > 1:1056181/1‑100 (MQ=32)
gATGTCGGCGCATCACATCCTGGAGCTGAAGTCGGTCCCAAGGGTGTGGCTGGTCGCCATTTAAAGTCGTACGGGAGCTAGGGTTAGAACGTCGTGAGAc < 2:1573326/100‑1 (MQ=11)
tGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAg > 1:785375/1‑100 (MQ=33)
tGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAg < 2:259504/100‑1 (MQ=33)
gTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGt > 1:90238/1‑100 (MQ=18)
tCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGtt < 2:2174816/100‑1 (MQ=32)
cGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTc < 1:594708/100‑1 (MQ=32)
gCTCATCACATCCTGGAGCTGAAGTAGGGCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCg < 2:1144943/99‑1 (MQ=17)
atcaCATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTccc < 1:972358/100‑1 (MQ=33)
tcaCATCCTGGAGCTAAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCt > 2:1140001/1‑100 (MQ=17)
caTCCTGGAGCTGAAGTAGGTCCCAAAGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATc < 2:1515661/100‑1 (MQ=18)
ccTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGc < 1:2211014/100‑1 (MQ=33)
ccTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGc < 2:332371/100‑1 (MQ=33)
tGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCg > 2:436004/1‑100 (MQ=32)
gAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTg < 2:2093572/100‑1 (MQ=31)
aGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTgg > 2:682177/1‑100 (MQ=11)
aGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTgg > 1:474870/1‑100 (MQ=11)
|
GGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGG > NC_000913/3946147‑3946327
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
