Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F212 I1 R1 174 80.0 3892211 96.7% 3763768 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,534,206 T→G R47R (CGT→CGG)  ptsI → PEP‑protein phosphotransferase of PTS system (enzyme I)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,534,2060TG93.1% 293.6 / 8.0 87R47R (CGT→CGG) ptsIPEP‑protein phosphotransferase of PTS system (enzyme I)
Reads supporting (aligned to +/- strand):  ref base T (4/2);  new base G (45/36);  total (49/38)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

AAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGC  >  NC_000913/2534109‑2534300
                                                                                                 |                                                                                              
aaGCTCTGCTTCTGAAAGAAGACGAATTTGTCATTGACCGGAAAAAAATGTCTGCCGTCCATGTTGATCATGAAGTTGATCGTTTTCTGAGCGGTCGGGc                                                                                              <  1:882803/100‑1 (MQ=255)
 aGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGTGcc                                                                                             <  1:1833079/100‑1 (MQ=255)
 aGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGcc                                                                                             >  1:967120/1‑100 (MQ=255)
 aGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGcc                                                                                             >  2:222765/1‑100 (MQ=255)
  gCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCa                                                                                            <  1:1661420/100‑1 (MQ=255)
  gCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCa                                                                                            <  2:210572/100‑1 (MQ=255)
   ctctGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCaa                                                                                           >  1:1562325/1‑100 (MQ=255)
     ctGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAgg                                                                                         <  1:1007069/100‑1 (MQ=255)
      tGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGc                                                                                        <  2:1213259/100‑1 (MQ=255)
       gCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCa                                                                                       >  2:1775775/1‑100 (MQ=255)
         ttCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCAtn                                                                                     <  1:333898/100‑2 (MQ=255)
         ttCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATc                                                                                     >  2:1714330/1‑100 (MQ=255)
          tCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCa                                                                                    >  1:45603/1‑100 (MQ=255)
             gAACGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTAGAACGGTTGCTGAGCGGTCGTGCCAAGGCATCAGc                                                                                  >  1:1340988/1‑99 (MQ=255)
             gAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGcc                                                                                 >  2:372976/1‑100 (MQ=255)
               aagaagACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCCGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCa                                                                               >  1:293540/1‑100 (MQ=255)
                 gaagaCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGc                                                                             >  1:1544018/1‑100 (MQ=255)
                 gaagaCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGc                                                                             <  2:1521120/100‑1 (MQ=255)
                 gaagaCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGAACAGGTTGATAAGGAAGTTGAACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGc                                                                             >  1:957654/1‑100 (MQ=255)
                  aagaCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCt                                                                            <  2:1565411/100‑1 (MQ=255)
                   agaCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTg                                                                           >  2:20687/1‑100 (MQ=255)
                   agaCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTg                                                                           >  1:1442439/1‑100 (MQ=255)
                    gaCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTgg                                                                          <  2:550038/100‑1 (MQ=255)
                    gaCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTgg                                                                          >  1:1303916/1‑100 (MQ=255)
                     aCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGa                                                                         >  2:1150050/1‑100 (MQ=255)
                        aaaTTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAAc                                                                      >  2:1515493/1‑100 (MQ=255)
                           ttGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGAt                                                                   <  2:1549713/100‑1 (MQ=255)
                            tGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATc                                                                  <  2:1461706/100‑1 (MQ=255)
                            tGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATc                                                                  >  1:1232773/1‑100 (MQ=255)
                               cATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATTAGGAAGTTGAACGTTTTCTGAGCGGTCGGGGCAAGGTATCAGCCCAGCTGGAAACGATCa                                                                 >  1:1161411/1‑98 (MQ=255)
                               cATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCaaa                                                               >  2:697862/1‑100 (MQ=255)
                               cATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCaaa                                                               <  2:198482/100‑1 (MQ=255)
                               cATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCaaa                                                               >  2:697968/1‑100 (MQ=255)
                                aTTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCaaaa                                                              <  2:1716135/100‑1 (MQ=255)
                                 ttGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAAc                                                             <  1:49554/100‑1 (MQ=255)
                                  tGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACg                                                            <  2:529881/100‑1 (MQ=255)
                                   gACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGa                                                           >  2:1779389/1‑100 (MQ=255)
                                    acaggAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCAAAGGCATCAGCCCAGCTGGAAACGATCAAAACGaa                                                          <  1:673298/97‑1 (MQ=255)
                                    aCCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGaa                                                          <  2:294097/100‑1 (MQ=255)
                                     ccGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGaaa                                                         <  2:276097/100‑1 (MQ=255)
                                       ggAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGc                                                       >  1:1682036/1‑100 (MQ=255)
                                        gAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCt                                                      >  2:845979/1‑100 (MQ=255)
                                         aaaaaaaTTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTg                                                     >  2:1942554/1‑100 (MQ=255)
                                           aaaaaTTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGt                                                   >  1:1282582/1‑100 (MQ=255)
                                           aaaaaTTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGt                                                   <  1:798283/100‑1 (MQ=255)
                                           aaaaaTTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGt                                                   >  2:1196350/1‑100 (MQ=255)
                                            aaaaTTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTg                                                  <  1:1942541/100‑1 (MQ=255)
                                            aaaaTTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTg                                                  >  2:1208511/1‑100 (MQ=255)
                                            aaaaTTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTg                                                  >  1:1390432/1‑100 (MQ=255)
                                                 ttCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACg                                             >  1:708791/1‑100 (MQ=255)
                                                   cTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGtt                                           >  2:1096922/1‑100 (MQ=255)
                                                     gCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCg                                         >  1:1591601/1‑100 (MQ=255)
                                                     gCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCg                                         <  2:45603/100‑1 (MQ=255)
                                                       cGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGt                                       <  2:1858119/100‑1 (MQ=255)
                                                       cGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGt                                       >  2:225441/1‑100 (MQ=255)
                                                       cGACCAGGTTGATCAGGAAGTGATACGTTTTCTGAGCGGTCGGGACAAGGAATCAGCCCATCTGGAAACGATCATAACCTACGCAGATGAAAc                                              >  1:1857292/1‑93 (MQ=255)
                                                         aCCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTga                                     <  1:247148/100‑1 (MQ=255)
                                                          ccAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTgaa                                    >  1:587674/1‑100 (MQ=255)
                                                          ccAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTgaa                                    >  2:1133091/1‑100 (MQ=255)
                                                             ggTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTgaagaa                                 >  2:1173300/1‑100 (MQ=255)
                                                             ggTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTgaagaa                                 >  1:343282/1‑100 (MQ=255)
                                                             ggTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTgaagaa                                 >  2:242618/1‑100 (MQ=255)
                                                                   tCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAaagaa                           <  2:592708/100‑1 (MQ=255)
                                                                    cAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAaagaag                          <  2:1705755/100‑1 (MQ=255)
                                                                     aGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGc                         <  2:1144726/100‑1 (MQ=255)
                                                                         aGTTGAACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATc                     >  2:1663410/1‑100 (MQ=255)
                                                                         aGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATc                     >  1:852214/1‑100 (MQ=255)
                                                                            tGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCttt                  >  1:338002/1‑100 (MQ=255)
                                                                            tGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCttt                  >  2:380605/1‑100 (MQ=255)
                                                                             gAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTg                 <  2:1041174/100‑1 (MQ=255)
                                                                             gAACGTTTTCTGAGCGGCCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTg                 >  1:1473170/1‑100 (MQ=255)
                                                                               aCGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGaa               <  1:1826672/100‑1 (MQ=255)
                                                                                cGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAg              <  1:1714319/100‑1 (MQ=255)
                                                                                 gTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAgg             >  1:1163702/1‑100 (MQ=255)
                                                                                  ttttCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAggg            <  1:129486/100‑1 (MQ=255)
                                                                                    ttCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCa          <  1:950390/100‑1 (MQ=255)
                                                                                    ttCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCa          <  2:1218097/100‑1 (MQ=255)
                                                                                      cTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCata        >  2:1698168/1‑100 (MQ=255)
                                                                                      cTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCata        >  1:1630403/1‑100 (MQ=255)
                                                                                      cTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCata        >  1:1615779/1‑100 (MQ=255)
                                                                                       tGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCatgt       >  2:314489/1‑98 (MQ=255)
                                                                                        gAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCa          <  1:1692264/96‑1 (MQ=255)
                                                                                         aGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCAtatta     <  1:697967/100‑1 (MQ=255)
                                                                                         aGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCAtatta     <  1:697861/100‑1 (MQ=255)
                                                                                          gCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCAtattat    <  1:1150048/100‑1 (MQ=255)
                                                                                           cGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAATGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTAtg   >  1:1241282/1‑100 (MQ=255)
                                                                                            ggTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTAtgc  <  2:1562336/100‑1 (MQ=255)
                                                                                                 |                                                                                              
AAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGC  >  NC_000913/2534109‑2534300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A