Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F212 I1 R1
|
174 |
80.0 |
3892211 |
96.7% |
3763768 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,805,475 |
T→C |
N159S (AAT→AGT) |
waaP ← |
kinase that phosphorylates core heptose of lipopolysaccharide |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,805,475 | 0 | T | C | 90.1%
| 205.6
/ 11.0
| 71 | N159S (AAT→AGT) | waaP | kinase that phosphorylates core heptose of lipopolysaccharide |
| Reads supporting (aligned to +/- strand): ref base T (2/5); new base C (28/36); total (30/41) |
| Fisher's exact test for biased strand distribution p-value = 6.91e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 |
CCGATGCAGATCGATAACTGAAATTTTTAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCGGGTTGACTGCCCAATCG > NC_000913/3805381‑3805570
|
ccGATGCAGATCGATAACTGAAATTTTTAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATc < 2:526163/100‑1 (MQ=255)
gCAGATCGATAACTGAAATTTTTAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGc < 1:1381305/100‑1 (MQ=255)
cAGATCGATAACTGAAATTTTTAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCa > 1:1940215/1‑100 (MQ=255)
aGATCGATAACTGAAATTTTTAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAg < 1:481659/100‑1 (MQ=255)
aTCGATAACTGAAATTTTTAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTa < 2:1492910/100‑1 (MQ=255)
tCGATAACTGAAATTTTTAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTAt > 2:1525193/1‑100 (MQ=255)
tCGATAACTGAAATTTTTAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTAt < 1:1439949/100‑1 (MQ=255)
cGATAACTGAAATTTTTAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATg < 1:1101697/100‑1 (MQ=255)
tAACTGAAATTTTTAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCAt > 1:70945/1‑100 (MQ=255)
cTGAAATTTTTAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCAtttt > 2:1884952/1‑100 (MQ=255)
cTGAAATATTTAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCAtttt > 2:1022725/1‑100 (MQ=255)
tGAAATTTTTAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTa < 2:70945/100‑1 (MQ=255)
gAAATTTTTAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCATTTTAc > 1:1383171/1‑100 (MQ=255)
gAAATTTTTAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTAc < 2:1488773/100‑1 (MQ=255)
gAAATTTTTAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTAc < 1:106151/100‑1 (MQ=255)
aaaTTTTTAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACg > 1:569958/1‑100 (MQ=255)
aaTTTTTAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACg < 2:57610/99‑1 (MQ=255)
aaTTTTTAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGc < 2:1646926/100‑1 (MQ=255)
aTTTTTAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCa > 2:1628635/1‑100 (MQ=255)
ttttAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCAcc > 1:506263/1‑100 (MQ=255)
ttttAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCAcc < 2:1480615/100‑1 (MQ=255)
ttttAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCAcc > 2:261949/1‑100 (MQ=255)
tttAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCa > 1:530546/1‑100 (MQ=255)
aaTTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAg > 1:642581/1‑100 (MQ=255)
aaTTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAg > 1:1891127/1‑100 (MQ=255)
tCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTg < 2:556208/100‑1 (MQ=255)
cATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGc > 2:168910/1‑100 (MQ=255)
cATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGc > 1:451879/1‑100 (MQ=255)
aTCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCt < 2:1885719/100‑1 (MQ=255)
aTCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCt > 2:749323/1‑100 (MQ=255)
cTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTac > 1:1720336/1‑100 (MQ=255)
cTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTac < 1:1530914/100‑1 (MQ=255)
tCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTacac > 1:1598259/1‑100 (MQ=255)
cGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTg < 1:1495262/100‑1 (MQ=255)
cGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTg < 2:1643577/100‑1 (MQ=255)
ggCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGc > 2:436913/1‑100 (MQ=255)
gCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCg < 1:1728470/100‑1 (MQ=255)
ccAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGa < 1:677033/100‑1 (MQ=255)
cAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGAt > 1:1505174/1‑100 (MQ=255)
aGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATc < 2:548928/100‑1 (MQ=255)
tAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAg < 1:1649561/100‑1 (MQ=255)
tAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAg < 2:1533743/100‑1 (MQ=255)
aaaTGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGc < 2:1616471/100‑1 (MQ=255)
aaTGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCa < 2:300250/100‑1 (MQ=255)
aaTGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCa < 1:851718/100‑1 (MQ=255)
cAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGc < 2:848866/100‑1 (MQ=255)
aaaTGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCt < 2:375513/100‑1 (MQ=255)
aaaTGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCt < 2:723074/100‑1 (MQ=255)
aaaTGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCt > 2:203257/1‑100 (MQ=255)
aTGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTa > 2:244443/1‑100 (MQ=255)
tGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTaa > 2:1273959/1‑100 (MQ=255)
gAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCCGTATGCATTTTACGCACCATAGTTGCTACACGTGCGTTCAGCATACGCTTa > 2:636409/1‑98 (MQ=255)
aaGCAAAAAGTGGCAAATGTAACAGGCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAaca < 1:858804/100‑1 (MQ=255)
aGCAAAAAGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAacac < 1:1525185/100‑1 (MQ=255)
aaaaaGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGta > 1:1071656/1‑100 (MQ=255)
aaaaaGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGta < 2:214913/100‑1 (MQ=255)
aaaaGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGta < 1:600669/99‑1 (MQ=255)
aGTGGCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATc < 1:688945/100‑1 (MQ=255)
ggCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAgg > 2:757449/1‑100 (MQ=255)
ggCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAgg > 1:1104886/1‑100 (MQ=255)
ggCAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAg > 1:1359628/1‑99 (MQ=255)
cAAATGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCg > 1:1344159/1‑100 (MQ=255)
aaTGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCggg < 1:252425/100‑1 (MQ=255)
aaTGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCggg < 2:569960/100‑1 (MQ=255)
aaTGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCggg < 2:711366/100‑1 (MQ=255)
aaTGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCggg > 2:804612/1‑100 (MQ=255)
aTGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCGGGt < 2:193396/100‑1 (MQ=255)
tGTAACAGTCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCGGGtt < 2:1940228/100‑1 (MQ=255)
tCGCGGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCGGGTTGACTGccc < 1:24199/100‑1 (MQ=255)
cgGTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCGGGTTGACTGCCCAAt < 2:1539952/100‑1 (MQ=255)
gTGACTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCGGGTTGACTGCCCAATCg < 2:506265/100‑1 (MQ=255)
|
CCGATGCAGATCGATAACTGAAATTTTTAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCGGGTTGACTGCCCAATCG > NC_000913/3805381‑3805570
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
