Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F212 I1 R1
|
174 |
80.0 |
3892211 |
96.7% |
3763768 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,152,760 |
G→T |
N113K (AAC→AAA) |
ppc ← |
phosphoenolpyruvate carboxylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,152,760 | 0 | G | T | 98.6%
| 243.4
/ ‑3.4
| 69 | N113K (AAC→AAA) | ppc | phosphoenolpyruvate carboxylase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (32/36); total (32/37) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01 |
ACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTCGGCGGTGTTG > NC_000913/4152664‑4152853
|
acgacgGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGtttt < 1:457245/100‑1 (MQ=255)
acgacgGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGtttt > 1:1487909/1‑100 (MQ=255)
cgacgGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTtttt > 1:1485685/1‑100 (MQ=255)
acgGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTc < 1:70748/100‑1 (MQ=255)
acgGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTc < 2:1659050/100‑1 (MQ=255)
acgGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTc > 1:445161/1‑100 (MQ=255)
cgGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCa < 2:66734/100‑1 (MQ=255)
gggTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAg < 2:1130795/100‑1 (MQ=255)
gggTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAg < 2:708331/100‑1 (MQ=255)
aaTTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTa > 1:1039879/1‑100 (MQ=255)
tttCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACg > 2:119954/1‑100 (MQ=255)
tttCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACg > 2:634751/1‑100 (MQ=255)
ttCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGc < 1:774672/100‑1 (MQ=255)
gggTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGc > 1:1711026/1‑100 (MQ=255)
gggTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGc < 2:329301/100‑1 (MQ=255)
gAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGc < 2:1909530/100‑1 (MQ=255)
gAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGc < 2:405102/100‑1 (MQ=255)
gCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCg > 1:178557/1‑99 (MQ=255)
gCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGa > 1:138652/1‑100 (MQ=255)
ccGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGAt < 2:195032/100‑1 (MQ=255)
cGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATc < 1:1345729/100‑1 (MQ=255)
gTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCa < 2:1487917/100‑1 (MQ=255)
gAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACt < 2:33816/100‑1 (MQ=255)
aCCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCg > 2:936441/1‑100 (MQ=255)
aCCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGAGGGCGATCACTTCCg > 2:1910410/1‑100 (MQ=255)
ccAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTTTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCg > 1:282936/1‑99 (MQ=255)
ccAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCgg > 2:1617114/1‑100 (MQ=255)
ctgTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCggg < 2:507747/98‑1 (MQ=255)
gTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGtt > 2:1505022/1‑100 (MQ=255)
gTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGtt < 2:1396781/100‑1 (MQ=255)
ttCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTg < 1:1738831/100‑1 (MQ=255)
tCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGc > 1:1162083/1‑100 (MQ=255)
cagcgacagcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTg < 1:279505/100‑1 (MQ=255)
cagcgacagcgaTTCCACTGCTTTTTTGATGGTATCTTCGCTCAGTTCCGGCTGTTTTTTCTGTTTTTGCGTGGTGCGGGCGATCACTTCCGGGTTGCt > 1:1172311/1‑99 (MQ=255)
agcgacagcgaTTCCACTGCTTTCTTGATGGTTTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCATATTTCGGGCGATCACTTCCGGCTTGCTgg > 1:1681633/1‑100 (MQ=255)
gcgacagcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGc < 2:163598/100‑1 (MQ=255)
cgacagcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCa < 2:585896/100‑1 (MQ=255)
gacagcgcTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCa < 2:575365/99‑1 (MQ=255)
cagcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCt > 2:262412/1‑100 (MQ=255)
cagcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCt > 2:461281/1‑100 (MQ=255)
gcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGGGGGCGATCGCTTCCGGGTTGCTGGCAGCTTc > 1:1311193/1‑100 (MQ=255)
cgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCg < 1:79919/100‑1 (MQ=255)
aTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGcc < 1:936440/100‑1 (MQ=255)
aTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGcc < 1:1910397/100‑1 (MQ=255)
cACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTc > 2:1478937/1‑100 (MQ=255)
aCTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCg > 2:224652/1‑100 (MQ=255)
tGCTTTTTTGATGGCGTCTGCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCTGGTTGCTGGCAGCTTCGCCTTTCGGc < 1:1323861/100‑1 (MQ=255)
gCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGc > 1:1743168/1‑99 (MQ=255)
gCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCg < 2:1534245/100‑1 (MQ=255)
cTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGa > 2:309667/1‑100 (MQ=255)
cTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGa < 2:623958/100‑1 (MQ=255)
ttttttGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGaa < 2:1863084/100‑1 (MQ=255)
ttttGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAAt < 2:55309/100‑1 (MQ=255)
ttttGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAAt < 1:1505014/100‑1 (MQ=255)
ttGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGc > 2:1466367/1‑100 (MQ=255)
gATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCtg < 1:1705977/100‑1 (MQ=255)
gATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCtg < 2:1711037/100‑1 (MQ=255)
aTGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCtgt > 2:1768360/1‑100 (MQ=255)
ggTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTgg < 2:1711504/100‑1 (MQ=255)
gtgtCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCGGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGt < 1:582383/100‑1 (MQ=255)
tgtCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTa > 2:1496972/1‑100 (MQ=255)
tattCGCTCAGTTACGGCTGTTTTTTCAGTTCACGCAGGGTGAGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTAtt > 2:1312862/3‑100 (MQ=255)
cTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTg > 1:1854833/1‑100 (MQ=255)
tCGCTCAGTTTCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCt < 2:262785/100‑1 (MQ=255)
cGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTc < 1:1453519/100‑1 (MQ=255)
cGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTc > 2:918924/1‑100 (MQ=255)
gCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTcg > 2:1570218/1‑100 (MQ=255)
cTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTcgg > 2:494906/1‑100 (MQ=255)
cTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTcgg < 2:1487268/100‑1 (MQ=255)
ccGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTCGGCGGTGtt > 2:1141184/1‑100 (MQ=255)
cGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTCGGCGGTGTTg < 2:1018982/100‑1 (MQ=255)
|
ACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTCGGCGGTGTTG > NC_000913/4152664‑4152853
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
