Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F256 I0 R1 328 102.7 5123138 93.4% 4785010 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,152,760 G→T 100% N113K (AAC→AAA)  ppc ← phosphoenolpyruvate carboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,152,7600GT99.0% 315.4 / ‑4.0 96N113K (AAC→AAA) ppcphosphoenolpyruvate carboxylase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (49/46);  total (50/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01

Only 100 of 101 total aligned reads displayed.

TACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTCGGCGGTGTTGGCCAGG  >  NC_000913/4152663‑4152859
                                                                                                 |                                                                                                   
tACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGttt                                                                                                   <  1:1288476/100‑1 (MQ=255)
tACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGttt                                                                                                   <  1:426376/100‑1 (MQ=255)
 acgacgGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGtttt                                                                                                  <  2:174669/100‑1 (MQ=255)
   gacgGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGtttttt                                                                                                <  1:276642/100‑1 (MQ=255)
    acgGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTc                                                                                               <  2:254388/100‑1 (MQ=255)
     cgGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTGTTTTc                                                                                               <  2:173957/99‑1 (MQ=255)
      gggTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAg                                                                                             <  1:2419565/100‑1 (MQ=255)
      gggTAATTTCGGTGGGGTGAGCCGTGAGGACCAGTTCCAGCGACATCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAg                                                                                             <  2:2508131/100‑1 (MQ=255)
       ggTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCCCTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGt                                                                                            >  2:289412/1‑100 (MQ=255)
       ggTAATTTCGGTTGGGTGAGCCGAGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAg                                                                                             >  2:768026/1‑99 (MQ=255)
        gTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGtt                                                                                           <  1:2077922/100‑1 (MQ=255)
        gTAATTTCGGTTGGGTGAGCCGTGAGGACCAGATCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGtt                                                                                           >  1:2426521/1‑100 (MQ=255)
          aaTTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTa                                                                                         >  1:470035/1‑100 (MQ=255)
           aTTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTAc                                                                                        >  1:943065/1‑100 (MQ=255)
           aTTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTAc                                                                                        <  1:1828569/100‑1 (MQ=255)
            tttCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGGCAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACg                                                                                       >  2:152070/1‑100 (MQ=255)
            tttCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACg                                                                                       <  2:1118778/100‑1 (MQ=255)
                 gTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTTTTTTTTTTTTTTCTGGGTTCCGGCTGTTTTTTCCGTTTACGCa                                                                                     >  2:2470418/1‑97 (MQ=255)
                  tggggTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTTATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGt                                                                                 <  2:2426601/98‑1 (MQ=255)
                     ggTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCg                                                                              >  1:312816/1‑100 (MQ=255)
                       tGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCggg                                                                            <  2:22911/100‑1 (MQ=255)
                       tGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCggg                                                                            >  2:1463841/1‑100 (MQ=255)
                         aGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCg                                                                          <  2:1419896/100‑1 (MQ=255)
                          gCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCg                                                                          >  2:490393/1‑99 (MQ=255)
                          gCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGa                                                                         >  1:704440/1‑100 (MQ=255)
                            cGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGCGCGATc                                                                       >  2:139133/1‑100 (MQ=255)
                             gTGATGACCAGTTCCAGCGACAGCGATTCCATTTCTTTTTTGATGGTGTCTTCTCTCAGTTCCGGCTTTTTTTTCAGTTTACGCAGGGTTCGGGCGATCa                                                                      <  2:187660/100‑1 (MQ=255)
                             gTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCa                                                                      <  1:2490722/100‑1 (MQ=255)
                             gTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCa                                                                      >  1:1647173/1‑100 (MQ=255)
                             gTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCa                                                                      >  1:423721/1‑100 (MQ=255)
                              tGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCAc                                                                     <  1:381548/100‑1 (MQ=255)
                              tGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCAc                                                                     >  2:517728/1‑100 (MQ=255)
                               gAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACt                                                                    >  1:760926/1‑100 (MQ=255)
                                 ggACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTc                                                                  >  1:1056762/1‑100 (MQ=255)
                                 ggACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTc                                                                  >  1:1782274/1‑100 (MQ=255)
                                 ggACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCGCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGGTCACtt                                                                   >  2:64137/1‑99 (MQ=255)
                                  gACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTcc                                                                 <  2:2103195/100‑1 (MQ=255)
                                   aCCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTTTTTTTTTTTTGGGTGCGGGCGATCACTTCCg                                                                >  2:716474/1‑100 (MQ=255)
                                   aCCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCg                                                                >  2:2079796/1‑100 (MQ=255)
                                   aCCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCg                                                                >  2:1219353/1‑100 (MQ=255)
                                   aCCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCg                                                                <  1:772245/100‑1 (MQ=255)
                                   aCCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCg                                                                <  1:2474378/100‑1 (MQ=255)
                                    ccAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCgg                                                               >  2:394035/1‑100 (MQ=255)
                                    ccAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCgg                                                               <  2:1168471/100‑1 (MQ=255)
                                    ccAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCgg                                                               <  1:2512350/100‑1 (MQ=255)
                                    ccAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGAGGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCg                                                                <  1:384684/99‑1 (MQ=255)
                                     cAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCggg                                                              <  2:1449536/100‑1 (MQ=255)
                                     cAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCggg                                                              <  2:1232400/100‑1 (MQ=255)
                                     cAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCggg                                                              <  1:1113684/100‑1 (MQ=255)
                                       gTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGtt                                                            <  2:2413623/100‑1 (MQ=255)
                                         tCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGc                                                          <  1:1606024/100‑1 (MQ=255)
                                         tCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGc                                                          <  1:1606671/100‑1 (MQ=255)
                                          ccAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCt                                                         <  2:1002412/100‑1 (MQ=255)
                                          ccAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCt                                                         <  2:1094573/100‑1 (MQ=255)
                                          ccAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCt                                                         >  1:1658185/1‑100 (MQ=255)
                                            agcgacagcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTgg                                                       >  1:2440056/1‑100 (MQ=255)
                                            agcgacagcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTgg                                                       <  1:699768/100‑1 (MQ=255)
                                             gcgacagcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGc                                                      >  1:1813611/1‑100 (MQ=255)
                                             atctcagcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGc                                                      <  1:1975484/96‑1 (MQ=255)
                                              cgacagcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCa                                                     <  1:2340119/100‑1 (MQ=255)
                                               gacagcgaTTCCGCTGCTTTTTTGGTGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTGAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCt                                                         <  2:1094672/95‑1 (MQ=255)
                                                 cagcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAgat                                                  >  2:1975470/1‑98 (MQ=255)
                                                   gcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTc                                                >  2:512167/1‑100 (MQ=255)
                                                   gcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTc                                                >  1:1496793/1‑100 (MQ=255)
                                                   gcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTc                                                >  1:369366/1‑100 (MQ=255)
                                                    cgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCg                                               <  2:1713557/100‑1 (MQ=255)
                                                    cgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCg                                               <  1:2345163/100‑1 (MQ=255)
                                                    cgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCg                                               >  2:1333684/1‑100 (MQ=255)
                                                      aTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGcc                                             <  1:636391/100‑1 (MQ=255)
                                                         ccACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTTTGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCttt                                          >  2:59775/1‑100 (MQ=255)
                                                          cACTGCTTTTTTGATGGTGTCTTCGCTCAGTGCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTc                                         <  2:1952553/100‑1 (MQ=255)
                                                           aCTGCTTTTTTTATTGTGTCTTCTCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCg                                        <  1:1109837/100‑1 (MQ=255)
                                                           aCTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCg                                        >  1:588037/1‑100 (MQ=255)
                                                            cTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCgg                                       >  2:1842426/1‑100 (MQ=255)
                                                            cTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCgg                                       <  1:614876/100‑1 (MQ=255)
                                                              gCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTCCGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGccgaaaggcg                                     <  2:1866377/100‑9 (MQ=255)
                                                              gCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCg                                     >  1:2500400/1‑100 (MQ=255)
                                                               cTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGTAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGa                                    >  1:531823/1‑100 (MQ=255)
                                                               cTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCg                                     >  1:1296445/1‑99 (MQ=255)
                                                                   tttGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATg                                >  2:2450805/1‑100 (MQ=255)
                                                                     tGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCt                              >  2:496574/1‑100 (MQ=255)
                                                                        tGGTGTCTTCGCGCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCtgtg                           <  1:1163164/100‑1 (MQ=255)
                                                                         ggTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTgg                          >  2:2054382/1‑100 (MQ=255)
                                                                         ggTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTgg                          <  1:512173/100‑1 (MQ=255)
                                                                          gtgtCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCATGGTGCGGGCGATAACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCttaggt                         >  2:387039/1‑95 (MQ=255)
                                                                          gtgtCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGt                         >  2:387897/1‑100 (MQ=255)
                                                                               ttCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGc                    <  1:1957111/100‑1 (MQ=255)
                                                                                  gCTCAGTTCCGGCTGTTTTTTCTGTTTTTTCATGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTTGGCTTTCGGCGAAATGCTGTGGTATTGCTc                  >  2:227764/1‑99 (MQ=255)
                                                                                  gCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTcg                 >  1:1674906/1‑100 (MQ=255)
                                                                                  gCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTcg                 >  1:227395/1‑100 (MQ=255)
                                                                                   cTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTcgg                <  1:1755303/100‑1 (MQ=255)
                                                                                    tCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTcggc               <  2:2486702/100‑1 (MQ=255)
                                                                                    tCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTcggc               >  2:2056365/1‑100 (MQ=255)
                                                                                    tCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTcggc               <  1:792972/100‑1 (MQ=255)
                                                                                       gTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTCGGCGgt            >  2:1746735/1‑100 (MQ=255)
                                                                                       gTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTCGGCGgt            >  1:2197347/1‑100 (MQ=255)
                                                                                         tCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTCGGCGgtgt          >  2:28547/1‑100 (MQ=255)
                                                                                          ccGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTCGGCGGTGtt         >  1:731666/1‑100 (MQ=255)
                                                                                           cGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTCGGCGGTGTTg        <  1:2323394/100‑1 (MQ=255)
                                                                                                 gTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTCGGCGGTGTTGGTCAgg  >  2:195425/1‑100 (MQ=255)
                                                                                                 |                                                                                                   
TACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTCGGCGGTGTTGGCCAGG  >  NC_000913/4152663‑4152859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A