Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F56 I0 R1 312 170.3 8276235 93.7% 7754832 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,867,164 C→T 20.2% A130T (GCG→ACG)  rpoS ← RNA polymerase, sigma S (sigma 38) factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,867,1640CT20.2% 412.6 / 96.4 204A130T (GCG→ACG) rpoSRNA polymerase, sigma S (sigma 38) factor
Reads supporting (aligned to +/- strand):  ref base C (86/76);  new base T (20/21);  total (107/97)
Fisher's exact test for biased strand distribution p-value = 7.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.03e-01

Only 100 of 212 total aligned reads displayed.

GGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACT  >  NC_000913/2867066‑2867263
                                                                                                  |                                                                                                   
  tttGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACcgtgcg                                                                                                  <  1:2134042/100‑1 (MQ=255)
    tGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACcgcgcg                                                                                                  <  1:2856498/98‑1 (MQ=255)
    tGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGa                                                                                                <  2:126401/100‑1 (MQ=255)
     ggTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGTGCGGan                                                                                               <  1:3637968/100‑2 (MQ=255)
     ggTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGTGCGGAt                                                                                               >  1:3949454/1‑100 (MQ=255)
     ggTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGAt                                                                                               <  2:930998/100‑1 (MQ=255)
      gTTCATAATCGCCCGTTGAAGCGTCTGGCGAATCGATCAGGTTGCGTATGTTGAGAAGCGGAACCCACGTTCCGGGTCAAACTTCTCTACCGTGCGGAt                                                                                               <  2:1179777/99‑1 (MQ=255)
      gTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATc                                                                                              >  1:3594049/1‑100 (MQ=255)
      gTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATc                                                                                              <  2:3061095/100‑1 (MQ=255)
      gTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATc                                                                                              <  2:3369313/100‑1 (MQ=255)
       ttCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGTGCGGATCa                                                                                             >  2:3708585/1‑100 (MQ=255)
         cATAATCGTCCGTTCAATAGTCTGGCGAATCCACCAGGTTGCGTATGTGGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCac                                                                                            <  2:3644421/99‑2 (MQ=255)
         cATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGTGCGGATCAGc                                                                                           >  2:3680772/1‑100 (MQ=255)
          aTAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGcc                                                                                          >  1:2667157/1‑100 (MQ=255)
            aaTCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGTGCGGATCAGcccc                                                                                        <  1:1411396/100‑1 (MQ=255)
             aTCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCa                                                                                       >  1:4071481/1‑100 (MQ=255)
               cGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAgg                                                                                     <  2:1955967/100‑1 (MQ=255)
                gCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGt                                                                                    <  2:2422045/100‑1 (MQ=255)
                gCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGt                                                                                    <  2:505824/100‑1 (MQ=255)
                   cGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGc                                                                                 >  2:1041106/1‑100 (MQ=255)
                    gTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGcc                                                                                >  2:2259496/1‑100 (MQ=255)
                     ttCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGTGCGGATCAGCCCCAGGTTGccc                                                                               <  2:1444625/100‑1 (MQ=255)
                     ttCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGccc                                                                               <  1:113825/100‑1 (MQ=255)
                      tCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCct                                                                              <  1:3203822/100‑1 (MQ=255)
                       cAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCctc                                                                             >  1:275655/1‑100 (MQ=255)
                         aTCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCtt                                                                           >  2:3116806/1‑100 (MQ=255)
                          tCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGTGCGGATCAGCCCCAGGTTGCCCTCTTc                                                                          <  2:1585465/100‑1 (MQ=255)
                          tCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGTGCGGATCAGCCCCAGGTTGCCCTCTTc                                                                          <  2:72748/100‑1 (MQ=255)
                           cGTCTGGCGAATCCATCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCg                                                                         <  1:461369/100‑1 (MQ=255)
                           cGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGGCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCg                                                                         <  1:1672481/100‑1 (MQ=255)
                            gTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGTGCGGATCAGCCCCAGGTTGCCCTCTTCGa                                                                        >  1:786546/1‑100 (MQ=255)
                            gTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGa                                                                        <  2:4070566/100‑1 (MQ=255)
                              cTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATa                                                                      >  1:3169450/1‑100 (MQ=255)
                               tGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATaa                                                                     >  1:1206653/1‑100 (MQ=255)
                               tGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATaa                                                                     >  2:439043/1‑100 (MQ=255)
                               tGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATaa                                                                     <  2:1438735/100‑1 (MQ=255)
                                ggCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAg                                                                    >  2:1699135/1‑100 (MQ=255)
                                ggCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAg                                                                    >  1:633102/1‑100 (MQ=255)
                                ggCGAATCCACCAGGCTGCGTATGTTGAGAAGCGGAAACCACGTTCCCGGCCAAACTTCTCTACCGCGCGGAACAGCCCCAGGTTGCCCTCTTCGATAAg                                                                    >  2:632331/1‑100 (MQ=255)
                                 gCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGTGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAgg                                                                   <  1:3529/100‑1 (MQ=255)
                                    aaTCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTca                                                                >  2:1004853/1‑99 (MQ=255)
                                    aaTCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGGCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTcc                                                                >  1:1481225/1‑100 (MQ=255)
                                      tCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGTGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAg                                                              >  2:838118/1‑100 (MQ=255)
                                      tCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGTTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAg                                                              >  1:2359149/1‑100 (MQ=255)
                                       ccaccaGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGTGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGc                                                             >  1:3756136/1‑100 (MQ=255)
                                       ccaccaGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCTGGTTGCCCTCTTCGATAAGGTCCAGc                                                             <  1:2515118/100‑1 (MQ=255)
                                        caccaGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCa                                                            <  1:4133792/100‑1 (MQ=255)
                                         accaGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCaa                                                           >  1:1607927/1‑100 (MQ=255)
                                          ccaGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAAc                                                          >  2:2554262/1‑100 (MQ=255)
                                           caGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACg                                                         <  2:3940933/100‑1 (MQ=255)
                                              gTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCa                                                      <  2:3225051/100‑1 (MQ=255)
                                                tGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGa                                                    >  1:1927789/1‑100 (MQ=255)
                                                 gCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGAc                                                   <  2:1734741/100‑1 (MQ=255)
                                                  cGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGTGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGAcc                                                  >  1:966457/1‑100 (MQ=255)
                                                    tATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCAc                                                <  2:3297473/100‑1 (MQ=255)
                                                     aTGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACg                                               >  2:30281/1‑100 (MQ=255)
                                                     aTGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACg                                               <  1:1017987/100‑1 (MQ=255)
                                                      tGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGa                                              <  2:2022473/100‑1 (MQ=255)
                                                       gTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGTGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGAt                                             <  2:125106/100‑1 (MQ=255)
                                                       gTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGAt                                             >  1:154898/1‑100 (MQ=255)
                                                        ttGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGTGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGAtt                                            >  1:439157/1‑100 (MQ=255)
                                                         tGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGTTCAGCCCCAGGTTGCCCTCTTCGATAAGGCCCAGCAACGCCAGACCACGAt                                             >  2:3843702/1‑98 (MQ=255)
                                                         tGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTg                                           >  1:1288785/1‑100 (MQ=255)
                                                          gagaAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGc                                          <  2:3525169/100‑1 (MQ=255)
                                                            gaAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCa                                        <  2:1234825/100‑1 (MQ=255)
                                                              aGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGTGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATa                                      >  1:1765060/1‑100 (MQ=255)
                                                              aGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATa                                      >  2:1502895/1‑100 (MQ=255)
                                                                 ggAAACCACGTTCCGGGTCAAACTTCTCTGCCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAAcg                                   <  2:2366242/100‑1 (MQ=255)
                                                                 ggAAACCACGTTCCGGGTCAAACTTCTCTACCGTGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAAcg                                   >  2:2668071/1‑100 (MQ=255)
                                                                 ggAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAAcg                                   >  2:2300547/1‑100 (MQ=255)
                                                                 ggAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAAcg                                   >  2:2187350/1‑100 (MQ=255)
                                                                   aaaCCAGGATCCTGGTCAAACTCCATTACCGCGCTGATCAGCCGTTGGTTGCGATCTTCGATAAGGTCCATCAACTACTGATCACGATTTTCATAAcg                                   <  2:3196465/98‑1 (MQ=255)
                                                                   aaaCCACGTTCCGGGTCAAACTTCTCTACCGTGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAAcggc                                 <  1:2546940/100‑1 (MQ=255)
                                                                      ccACGTTCCGGGTCAAACTTCTCTACCTCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCATCAACGCCAGACCACGATTGCCATAACGGCggg                              <  2:2459657/100‑1 (MQ=255)
                                                                      ccACGTTCCGGGTCAAACTTCTCTACCGTGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCggg                              >  1:472405/1‑100 (MQ=255)
                                                                      ccACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCggg                              >  2:2735086/1‑100 (MQ=255)
                                                                       cACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGc                             <  1:3651710/100‑1 (MQ=255)
                                                                       cACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACCCCAGACCACGATTACCATAACGGCGGGc                             >  1:2054053/1‑100 (MQ=255)
                                                                          gTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAAcggcgg                               >  2:866695/1‑95 (MQ=255)
                                                                           ttCCGGGTCAAACTTCTCTACCGTGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAAtt                         >  2:2924184/1‑100 (MQ=255)
                                                                            tCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAAttn                        <  2:154897/100‑2 (MQ=255)
                                                                              cGGGTCAAACTTCTCTACCGTGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAAttttt                      <  1:221122/100‑1 (MQ=255)
                                                                              cGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAAttttt                      <  2:1803375/100‑1 (MQ=255)
                                                                               gggTCAAACTTCTCTACCGTGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTa                     <  1:2887138/100‑1 (MQ=255)
                                                                               gggTCAAACTTCTCTACCGTGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTa                     <  1:2887472/100‑1 (MQ=255)
                                                                               gggTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTa                     >  2:2121640/1‑100 (MQ=255)
                                                                                 gTCAAACTTCTCTACCGTGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTacc                   >  1:1378962/1‑100 (MQ=255)
                                                                                 gTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTacc                   <  2:3768516/100‑1 (MQ=255)
                                                                                   cAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTaccac                 >  1:3266249/1‑100 (MQ=255)
                                                                                   cAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTacca                  >  2:200168/1‑99 (MQ=255)
                                                                                    aaaCTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTaccacc                <  1:404739/100‑1 (MQ=255)
                                                                                    aaaCTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTaccacc                >  1:3381733/1‑100 (MQ=255)
                                                                                     aaCTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTAccacca               <  1:2972120/100‑1 (MQ=255)
                                                                                      aCTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAg              <  1:2246279/100‑1 (MQ=255)
                                                                                       cTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGa             >  1:666897/1‑100 (MQ=255)
                                                                                       cTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGa             <  2:2866023/100‑1 (MQ=255)
                                                                                        ttCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAGCGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGAc            <  2:3565129/100‑1 (MQ=255)
                                                                                              aCCGCGCTGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTTCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGt      <  2:2216934/100‑1 (MQ=255)
                                                                                                cgcgcgGTTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTa    <  1:856709/100‑1 (MQ=255)
                                                                                                  cgcgGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTAct  >  1:717586/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
GGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACT  >  NC_000913/2867066‑2867263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A