Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F56 I0 R1	312	170.3	8276235	93.7%	7754832	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,992,747	A→C	46.3%	E218D (GAA→GAC)	ygeH →	putative transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,992,747	0	A	C	46.3%	39.0 / 276.1	192	E218D (GAA→GAC)	ygeH	putative transcriptional regulator
Reads supporting (aligned to +/- strand): ref base A (48/55); new base C (45/44); total (93/99)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 200 total aligned reads displayed.

GAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGACCAAGGATATAAG  >  NC_000913/2992650‑2992846

     aaCCAGAATAATGCAGTGAACACTTTATACATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGACCTACata                                                                                              >  1:2923428/1‑100 (MQ=255)

         aGAATAATGCAGTGAACACTTTATACATTGAGTTGATCGACGCTAAAAACCTTTACCTCATCGCCCGTGATCATCTCCCTGTTGATGAACTACataata                                                                                           <  2:2713288/99‑1 (MQ=255)

          gAATAATGCAGTGAACACTTTATACATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACa                                                                                         <  1:562197/100‑1 (MQ=255)

           aataatGCAGTGAACACTTTATACATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGACCGACACAATACAt                                                                                        <  2:2169081/100‑1 (MQ=255)

            ataatGCAGTGAACACTTTATACATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATc                                                                                       <  2:2275782/100‑1 (MQ=255)

                tGCAGTGAACACTTTATACATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACaa                                                                                   <  1:1740100/100‑1 (MQ=255)

                 gCAGTGAACACTTTATACATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAAt                                                                                  <  1:2666313/100‑1 (MQ=255)

                    gTGAACACTTTATACATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATtta                                                                               <  2:2749421/100‑1 (MQ=255)

                      gAACACTTTATACATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATttatt                                                                             >  1:1766641/1‑100 (MQ=255)

                        acacTTTATACATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTtattat                                                                           <  1:484250/100‑1 (MQ=255)

                         cacTTTATACATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGACCTACATAATACATCACAATTTATtata                                                                          >  1:3862036/1‑100 (MQ=255)

                          acTTTATACATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAg                                                                         <  1:2262352/100‑1 (MQ=255)

                          acTTTATACATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTACCTGTTGATGAACTACATAATACATCACAATTTATTATAg                                                                         <  1:3667454/100‑1 (MQ=255)

                           cTTTATACATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGa                                                                        <  2:3217125/100‑1 (MQ=255)

                           cTTTATACATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGa                                                                        <  2:655230/100‑1 (MQ=255)

                           cTTTATACATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGa                                                                        <  1:1359537/100‑1 (MQ=255)

                           cTTTATACATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGa                                                                        <  2:2009703/100‑1 (MQ=255)

                            tttATACATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGACCTACATAATACATCACAATTTATTATAGat                                                                       <  1:1244697/100‑1 (MQ=255)

                              tataCATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGataa                                                                     >  2:796920/1‑100 (MQ=255)

                                taCATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGataata                                                                   >  1:969942/1‑100 (MQ=255)

                                taCATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGataata                                                                   >  1:1301810/1‑100 (MQ=255)

                                taCATTGAGTTGATCGACGCTAAAAACCTTTTCCTAATCGCCAGTAATCATCTCCCTTTTGATGAACTACATAATACATCACAATTTATTATAGataa                                                                     >  2:2908040/1‑98 (MQ=255)

                                  cATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATc                                                                 >  1:719666/1‑100 (MQ=255)

                                  cATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATc                                                                 <  2:3200753/100‑1 (MQ=255)

                                  cATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATc                                                                 >  2:3937028/1‑100 (MQ=255)

                                   aTTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATcc                                                                <  2:631286/100‑1 (MQ=255)

                                   aTTGAGTTGATCGACGCTAAAAACCTTTGCCTCATCGCCAGTAATCATCTCCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATcc                                                                <  2:276878/100‑1 (MQ=255)

                                    ttGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATCCt                                                               >  2:3117092/1‑100 (MQ=255)

                                       ggTTGATCGACACTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGATCATAACCTACATAATACATCACAATTTCTTATAGATAATATCCTTCa                                                            >  2:3851176/2‑100 (MQ=255)

                                       aGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGCCCTACATAATCCCTCCCCACTCATTATAGATAATATCCTTCa                                                            >  2:3981409/1‑100 (MQ=255)

                                         ttGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCaaa                                                          <  2:1360594/100‑1 (MQ=255)

                                          tGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAAc                                                         >  2:1515512/1‑100 (MQ=255)

                                           gttCGACGCTAAAGACCTTTTCCTCATCGCCAGTAATCATCTGCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACg                                                        >  1:2799087/3‑100 (MQ=255)

                                             tCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACgg                                                       <  2:1612654/99‑1 (MQ=255)

                                               gACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTAc                                                    >  2:1816509/1‑100 (MQ=255)

                                               gACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTAc                                                    <  2:6027/100‑1 (MQ=255)

                                               gACGCTAAAAACCTTTTCCGCATCGCCAGTAATGATCTACCTGTTTATGAACTACATAATACATCACACTTTATTATAGATAATATCCTTCAAACGATa                                                     >  2:2544174/1‑99 (MQ=255)

                                                aCGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACa                                                   <  1:1836217/100‑1 (MQ=255)

                                                 cGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACa                                                   >  2:1126358/1‑99 (MQ=255)

                                                 cGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACAt                                                  <  2:1766629/100‑1 (MQ=255)

                                                 cGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACAt                                                  <  2:711358/100‑1 (MQ=255)

                                                  gCTAAAAACCTTTTCCTCATCGCCAGTACTCACCTCCCTGTTGATAACCTACATAATACATCACAATTTATTATAGATAATATCTTTCAAACGGTACATa                                                 >  2:4069449/1‑100 (MQ=255)

                                                  acTAAAAACCTTTTCCTCCTCGTCGGTAATAATCTCCCTTTTGATGAACTACATAAAACACCACAATTTATTATAGATAATATCCTTCAACCGGTa                                                     <  2:1921273/95‑1 (MQ=255)

                                                   cTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATaa                                                >  1:2943427/1‑100 (MQ=255)

                                                    tAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATaaa                                               <  1:4009455/100‑1 (MQ=255)

                                                     aaaaaCCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAAc                                              >  1:1551761/1‑100 (MQ=255)

                                                     aaaaaCCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAAc                                              >  1:3529905/1‑100 (MQ=255)

                                                     aaaaaCCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAAc                                              >  1:2975886/1‑100 (MQ=255)

                                                     aaaaaCCTTTTCCTCATCGCCAGTAATCATCTCCCTGCTGATGAACTACATAATACATCACAATTTATTATAAATAATATCCTTCAAACGATACATaaa                                               >  1:1034830/1‑99 (MQ=255)

                                                       aaaCCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCa                                            >  1:3796853/1‑100 (MQ=255)

                                                        aaCCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAAcc                                             <  1:3222393/98‑1 (MQ=255)

                                                        aaCCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAg                                           <  1:1908615/100‑1 (MQ=255)

                                                          ccTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTGCATAAACCAGaa                                         >  1:3436230/1‑100 (MQ=255)

                                                          ccTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGaa                                         >  1:52340/1‑100 (MQ=255)

                                                          ccTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGaa                                         >  1:1449369/1‑100 (MQ=255)

                                                           cTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAAc                                        >  1:2432774/1‑100 (MQ=255)

                                                           cTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGaan                                        <  2:3142236/100‑2 (MQ=255)

                                                           cTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAAc                                        >  2:3015621/1‑100 (MQ=255)

                                                           cTTTTCCTAATCGCCAGTAATCGTCTCCCTGTCGATGCCCTACATACTACACCACTAACTATTACATATAATATCCTTCGTACGGTACATAAACCa                                            >  2:513742/1‑96 (MQ=255)

                                                            ttttCCACATCGCCAGTAATCATCGTCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAAc                                        <  1:1674348/99‑1 (MQ=255)

                                                             tttCCTCATCGCCAGTAATCATCTCCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGa                                      >  1:1939943/1‑100 (MQ=255)

                                                                 cTCATCGCCAGTAATCATCTCCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTg                                  <  1:1730576/100‑1 (MQ=255)

                                                                 cTCATCGCCAGTAATCATCTCCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTg                                  >  1:3337856/1‑100 (MQ=255)

                                                                 cTCATCGCCAGTAATCATCTCCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTg                                  >  1:4051088/1‑100 (MQ=255)

                                                                 cTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTg                                  >  2:875240/1‑100 (MQ=255)

                                                                 cTCATCGCCAATAATCATCTCCCTGTTTATGACCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTg                                  <  2:3079259/100‑1 (MQ=255)

                                                                  tCATCGCCAGTAATCATCTCCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGt                                 <  1:4129474/100‑1 (MQ=255)

                                                                  tCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGt                                 >  1:394129/1‑100 (MQ=255)

                                                                   cATCGCCAGTAATCATCTCCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTa                                <  2:3255931/100‑1 (MQ=255)

                                                                   cATCGCCAGTAATCATCTCCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTa                                <  2:3565656/100‑1 (MQ=255)

                                                                   cATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTa                                <  2:1279400/100‑1 (MQ=255)

                                                                    aTCGCCAGTAATCATCTCCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTaa                               <  2:264986/100‑1 (MQ=255)

                                                                     tCGCCAGTAATCATCTCCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAg                              <  2:1551749/100‑1 (MQ=255)

                                                                     tCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATATATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAg                              <  2:2492477/100‑1 (MQ=255)

                                                                     tCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAg                              >  1:3846440/1‑100 (MQ=255)

                                                                       gCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGAt                            >  2:1131009/1‑100 (MQ=255)

                                                                        ccAGTAATCATCTCCTTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACCTACACCAGAACGATCTGTAAGAtt                           <  2:3640661/100‑1 (MQ=255)

                                                                          aGTAATCATCTCCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAg                         >  1:3255980/1‑100 (MQ=255)

                                                                           gTAATCATCTCCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGc                        <  1:3110023/100‑1 (MQ=255)

                                                                            tAATCATCTCCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCt                       <  2:3334281/100‑1 (MQ=255)

                                                                               tcatcTCCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAg                    >  1:3397847/1‑100 (MQ=255)

                                                                               tcatcTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACTTAAACCAGAACGATCTGGAAGATTAGCTatg                    <  1:2787531/100‑3 (MQ=255)

                                                                               tcatcTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAg                    <  2:2408037/100‑1 (MQ=255)

                                                                                catcTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAg                    >  2:3241798/1‑99 (MQ=255)

                                                                                catcTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGc                   >  1:781628/1‑100 (MQ=255)

                                                                                    tcCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGa               <  1:2628835/100‑1 (MQ=255)

                                                                                    tcCCTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGa               >  2:1398733/1‑100 (MQ=255)

                                                                                     cccTGTTGATGACCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGAc              <  2:2533913/100‑1 (MQ=255)

                                                                                     cccTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGAc              >  2:2739614/1‑100 (MQ=255)

                                                                                       cTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGACCa            >  1:606634/1‑100 (MQ=255)

                                                                                        tGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGACCa            <  1:902533/99‑1 (MQ=255)

                                                                                         gTTGATGACCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGACCAAg          <  1:2481990/100‑1 (MQ=255)

                                                                                           tgatgaCCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGACCAAGGa        >  1:3179084/1‑100 (MQ=255)

                                                                                            gatgaACTACATCATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGACCAAGGat       >  1:2723388/1‑100 (MQ=255)

                                                                                             atgaCCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGACCAAGGata      <  1:2022519/100‑1 (MQ=255)

                                                                                              tgaACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAGCCAGAACGATCTGTAAGATTAGCTAAGCAGGACCAAGGatat     >  2:3108676/1‑100 (MQ=255)

                                                                                                naCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACAAGAACGATCTGTAAGATTAGCTAAGCAGGACCAAGGATATaa   >  2:844106/2‑100 (MQ=255)

                                                                                                aaCTACATAATAGATCACAATTTATTATAGATAAGATCCTTCACACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGACCAAGGATATaa   <  1:804273/100‑1 (MQ=255)

                                                                                                 aCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGACCAAGGATATAAg  <  1:2116226/100‑1 (MQ=255)

                                                                                                 aCTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGACCAAGGATATAAg  >  2:2116213/1‑100 (MQ=255)

GAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGACCAAGGATATAAG  >  NC_000913/2992650‑2992846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A