Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F133 I1 R1
|
102 |
83.9 |
4092546 |
96.1% |
3932936 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,580,128 |
A→G |
F221F (TTT→TTC) |
ydeM ← |
putative YdeN‑specific sulfatase‑maturating enzyme |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,580,128 | 0 | A | G | 96.0%
| 249.0
/ 0.3
| 75 | F221F (TTT→TTC) | ydeM | putative YdeN‑specific sulfatase‑maturating enzyme |
| Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (40/32); total (41/34) |
| Fisher's exact test for biased strand distribution p-value = 5.87e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01 |
CCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTC > NC_000913/1580032‑1580223
|
cccATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAAc > 1:574608/1‑100 (MQ=255)
cccATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAAc > 1:94605/1‑100 (MQ=255)
cccATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAAc < 1:1188084/100‑1 (MQ=255)
cATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACtt < 1:802391/100‑1 (MQ=255)
aTTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTg < 1:142853/100‑1 (MQ=255)
ttCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGc < 1:946684/100‑1 (MQ=255)
ccccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCa < 2:1662313/100‑1 (MQ=255)
cccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCAt > 1:844149/1‑100 (MQ=255)
cccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCAt > 1:1753485/1‑100 (MQ=255)
cAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTGTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATaa > 2:1432552/1‑100 (MQ=255)
aaaaaCGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGc < 1:691071/100‑1 (MQ=255)
aaaaaCGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGc > 1:1222518/1‑100 (MQ=255)
aCGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTg > 2:1296022/1‑100 (MQ=255)
ggCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTggg < 2:1782576/100‑1 (MQ=255)
gCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGa < 2:844146/100‑1 (MQ=255)
gCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGa < 2:432905/100‑1 (MQ=255)
cTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAg < 1:1607943/100‑1 (MQ=255)
aCAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGca > 1:597405/1‑100 (MQ=255)
cAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGcac < 1:1296029/100‑1 (MQ=255)
cAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGcac < 2:224571/100‑1 (MQ=255)
cAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTG‑TTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGcaca < 1:1310851/100‑1 (MQ=255)
aaGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAg < 2:94604/100‑1 (MQ=255)
aaGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAg > 1:1211781/1‑100 (MQ=255)
cTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGaaa > 1:1256779/1‑100 (MQ=255)
tttCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGaaaa > 1:406165/1‑100 (MQ=255)
ttCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGaaaaa < 2:597403/100‑1 (MQ=255)
ttCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGaaaaa > 2:135578/1‑100 (MQ=255)
aCTGACGGATGAAAATTTCACCCACATTGTTTTTAATCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAAt < 1:1432561/100‑1 (MQ=255)
aCTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAAt < 1:1869289/100‑1 (MQ=255)
aCTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAAt < 1:111884/100‑1 (MQ=255)
aCTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAAt < 1:1675245/100‑1 (MQ=255)
ctaaCGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAAt < 2:1800209/96‑1 (MQ=255)
tGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGa > 1:510455/1‑100 (MQ=255)
gACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGa < 1:132761/99‑1 (MQ=255)
gACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGAt > 1:511771/1‑100 (MQ=255)
gACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGAt > 1:805254/1‑100 (MQ=255)
gAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGaa > 1:440158/1‑99 (MQ=255)
aaaTTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAAtgt < 2:1104780/100‑1 (MQ=255)
tttCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGtt < 2:406164/100‑1 (MQ=255)
ttCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTtc > 1:80079/1‑100 (MQ=255)
tCACCCACATTGTTTTTAACCCATTGCATAAAAAGGGTTAACATGAACGTGCAATAAGACGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTtct < 2:440157/100‑1 (MQ=255)
aCCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCa > 1:537280/1‑100 (MQ=255)
cccACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCAc > 1:1100438/1‑100 (MQ=255)
cccACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCAc > 1:393406/1‑100 (MQ=255)
ccACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACt > 1:277806/1‑100 (MQ=255)
acaTTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTAt < 2:1874468/100‑1 (MQ=255)
attgTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGa > 1:1579089/4‑100 (MQ=255)
attgTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGa > 1:434984/4‑100 (MQ=255)
tgTTTTTAACCCATTGGATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGAcc < 2:556210/99‑1 (MQ=255)
cGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGAcc < 2:1222512/100‑1 (MQ=255)
gTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCa > 2:1823763/1‑100 (MQ=255)
ttttAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACt > 2:409530/1‑100 (MQ=255)
tttAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTg < 2:511770/100‑1 (MQ=255)
tttAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTg < 2:1753471/100‑1 (MQ=255)
ttAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGa < 2:823800/100‑1 (MQ=255)
aaCCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGaaa > 2:1224684/1‑100 (MQ=255)
cccATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATc < 1:1957585/100‑1 (MQ=255)
cATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCaa > 1:1546229/1‑100 (MQ=255)
gCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATAtt > 2:1947016/1‑100 (MQ=255)
gCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATAtt > 2:818605/1‑100 (MQ=255)
cATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTc > 2:430471/1‑100 (MQ=255)
aTAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCGATATTCg > 1:403923/1‑100 (MQ=255)
aTAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCg < 2:277805/100‑1 (MQ=255)
aaaaTGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCg > 2:951807/1‑100 (MQ=255)
aaaaTGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCg > 2:329854/1‑100 (MQ=255)
aTGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTcc < 1:2016937/100‑1 (MQ=255)
gTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCgg > 2:269512/1‑100 (MQ=255)
ttGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGt > 1:912347/1‑100 (MQ=255)
ttGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGt > 1:912920/1‑100 (MQ=255)
tGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGtt < 2:662963/100‑1 (MQ=255)
gACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGttt > 2:770126/1‑100 (MQ=255)
gACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGttt > 1:843371/1‑100 (MQ=255)
gACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGttt > 1:1458365/1‑100 (MQ=255)
aCATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTc > 2:1658732/1‑100 (MQ=255)
aCATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTc > 1:1001344/1‑100 (MQ=255)
|
CCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTC > NC_000913/1580032‑1580223
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
