Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F133 I0 R1	200	171.4	8351354	94.3%	7875326	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,580,128	A→G	21.5%	F221F (TTT→TTC)	ydeM ←	putative YdeN‑specific sulfatase‑maturating enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,580,128	0	A	G	21.5%	210.1 / 55.4	111	F221F (TTT→TTC)	ydeM	putative YdeN‑specific sulfatase‑maturating enzyme
Reads supporting (aligned to +/- strand): ref base A (40/47); new base G (12/12); total (52/59)
Fisher's exact test for biased strand distribution p-value = 8.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 113 total aligned reads displayed.

TGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGC  >  NC_000913/1580030‑1580227

tGCCCATTCCCCACAAAACGGCTGACAAAGCTTTCACACTGCCGGATGAAAATTTCACCCACGTTGTTTTTAACCCATTGCATAAACATGGTTGACATaa                                                                                                    >  2:3818138/1‑100 (MQ=255)

  cccATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAAc                                                                                                  >  1:2729861/1‑100 (MQ=255)

   ccATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACt                                                                                                 >  1:715835/1‑100 (MQ=255)

   ccATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACt                                                                                                 <  2:2283407/100‑1 (MQ=255)

   ccATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTCACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACt                                                                                                 <  2:2805150/100‑1 (MQ=255)

    cATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACtt                                                                                                >  1:3565130/1‑100 (MQ=255)

    cATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACtt                                                                                                <  1:2368642/100‑1 (MQ=255)

     aTTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTg                                                                                               <  1:1242278/100‑1 (MQ=255)

     aTTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTg                                                                                               <  1:2672870/100‑1 (MQ=255)

     aTTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTg                                                                                               <  2:3934157/100‑1 (MQ=255)

      ttCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGc                                                                                              <  2:1770429/100‑1 (MQ=255)

       tCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGcc                                                                                             <  1:3694681/100‑1 (MQ=255)

       tCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGcc                                                                                             <  2:1668141/100‑1 (MQ=255)

        ccccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCa                                                                                            >  2:2509626/1‑100 (MQ=255)

        ccccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCa                                                                                            >  2:188342/1‑100 (MQ=255)

         cccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATACAAATGGTTGACATAAAATTGCCAt                                                                                           >  1:3078173/1‑100 (MQ=255)

         cccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCAt                                                                                           >  1:3339039/1‑100 (MQ=255)

          ccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATa                                                                                          >  1:2465664/1‑100 (MQ=255)

          ccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATa                                                                                          >  1:471864/1‑100 (MQ=255)

          ccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATa                                                                                          >  1:135481/1‑100 (MQ=255)

           cAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCaaga                                                                                         >  1:4097861/1‑97 (MQ=255)

           cAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATaa                                                                                         >  2:1000452/1‑100 (MQ=255)

             aaaaaCGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGc                                                                                       <  2:2649407/100‑1 (MQ=255)

             aaaaaCGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGCTGACATAAACTTGCCATAAGc                                                                                       >  2:2847688/1‑100 (MQ=255)

                  cGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTgg                                                                                  <  1:128478/100‑1 (MQ=255)

                   ggCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTggg                                                                                 >  1:1289417/1‑100 (MQ=255)

                    gCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGa                                                                                <  1:33866/100‑1 (MQ=255)

                     cTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAg                                                                               <  1:2188909/100‑1 (MQ=255)

                      tGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAgg                                                                              <  1:3591353/100‑1 (MQ=255)

                      tGACAAAGCTTTCAAACTGACGGAGGAAAATTTCACCCACATTGGTCTTAACCCATTGCGTAAAAATGGTTTACATAAACTTGCCATAGGCCGTGGGAgg                                                                              <  2:1216360/100‑1 (MQ=255)

                       gACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGc                                                                             <  1:210167/100‑1 (MQ=255)

                       gACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGc                                                                             <  1:2509641/100‑1 (MQ=255)

                       gACAAAGCTTTCAAACTGACGGATGAAAAATTCCCACACATTGTTTTTAACCCATTGCTTAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGc                                                                             <  2:877321/100‑1 (MQ=255)

                        aCAAAGCTTTCAAACTGACGGATGAAAATTTTATCCACATTGTTTTTAAAAAAAAAAAAAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGc                                                                             <  2:2319353/99‑1 (MQ=255)

                        aCAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGca                                                                            <  2:1022756/100‑1 (MQ=255)

                         cAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGcac                                                                           >  1:419443/1‑100 (MQ=255)

                         cAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGcac                                                                           >  1:962810/1‑100 (MQ=255)

                           aaGCTTTCAAACTGACGGATGAAAATTTCACTCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAg                                                                         >  1:2813713/1‑100 (MQ=255)

                           aaGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAg                                                                         <  2:2041895/100‑1 (MQ=255)

                           aaGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAg                                                                         >  1:2107611/1‑100 (MQ=255)

                           aaGCTTTCAAACTAACGGATGAAAATTTCACGCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAg                                                                         >  1:2813667/1‑100 (MQ=255)

                              cTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGaaa                                                                      >  1:2255556/1‑100 (MQ=255)

                              cTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGaaa                                                                      >  2:457966/1‑100 (MQ=255)

                                   aaaCTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCa                                                                 <  2:1599438/100‑1 (MQ=255)

                                     aCTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAAt                                                               <  1:2003431/100‑1 (MQ=255)

                                     aCTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAAt                                                               <  1:367654/100‑1 (MQ=255)

                                      cTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATg                                                              <  2:2936183/100‑1 (MQ=255)

                                           aaataaaaaTTTCTCGTACATTTTGTTTAACCCATTGCATAAAAATGGCTAACATAAACTTGCCATAAGCCGTGAGTTTCACAGAAAAATCAATGacac                                                          <  2:4085912/94‑1 (MQ=255)

                                              tGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGaa                                                      >  1:2379546/1‑100 (MQ=255)

                                               gAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGaa                                                      >  2:3463612/1‑99 (MQ=255)

                                                aaaaTTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGAGAGGCACAGAAAAATCAATGATACGGAAtg                                                    >  2:3108142/1‑100 (MQ=255)

                                                     ttCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTtc                                               <  2:1130236/100‑1 (MQ=255)

                                                     atCACCAACATTCTTTTTAACAAAATGAATAAAAACGGATGACATAAACATGCAATCAGACGTGAAAAGCACAGAAAAATCAATGATACGGaaagaaaac                                               <  2:3749303/99‑8 (MQ=255)

                                                        aCCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCa                                            <  2:1045542/100‑1 (MQ=255)

                                                        aCCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCa                                            <  2:1045760/100‑1 (MQ=255)

                                                        aCCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCa                                            >  2:2121009/1‑100 (MQ=255)

                                                        aCCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCa                                            <  1:1957417/100‑1 (MQ=255)

                                                         cccACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCAc                                           >  1:783226/1‑100 (MQ=255)

                                                           cacaTTGTTTTTAACCCATTGCATACAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATCCGGAATGTGTTCTCACTa                                         >  2:3869361/1‑100 (MQ=255)

                                                           cacaTTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTa                                         <  2:155528/100‑1 (MQ=255)

                                                           cacaTTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTa                                         >  1:473578/1‑100 (MQ=255)

                                                           cacaTTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTa                                         <  1:1979905/100‑1 (MQ=255)

                                                            acaTTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTAt                                        >  1:2485602/1‑100 (MQ=255)

                                                             cattgTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATg                                       <  2:2729845/96‑1 (MQ=255)

                                                              attgTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGa                                      >  1:1764353/4‑100 (MQ=255)

                                                              attgTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGa                                      >  2:3757729/4‑100 (MQ=255)

                                                               ttgTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGAc                                     <  1:188345/98‑1 (MQ=255)

                                                                tgTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGAcc                                    <  2:1542776/99‑1 (MQ=255)

                                                                tgTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGAcc                                    <  1:3087683/99‑1 (MQ=255)

                                                                tgTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGAcc                                    <  2:3524826/99‑1 (MQ=255)

                                                                  tttttAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCAc                                  >  2:774632/1‑100 (MQ=255)

                                                                   ttttAACCCATTGCATAAAAATGGTTGACAAGAACTGGCCATAAGCGGGGGGAGGCACAGAAAAATCAATGATACGGCAGGTGTGCTCACTATGACCACt                                 >  2:1385209/1‑100 (MQ=255)

                                                                    tttAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTg                                <  1:2487867/100‑1 (MQ=255)

                                                                    tttAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTg                                <  1:3867156/100‑1 (MQ=255)

                                                                        aCCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAAt                            <  1:3164045/100‑1 (MQ=255)

                                                                         cccATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATc                           >  1:3766648/1‑100 (MQ=255)

                                                                         cccATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATc                           <  1:1291001/100‑1 (MQ=255)

                                                                           cATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCaa                         <  2:1994114/100‑1 (MQ=255)

                                                                           cATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCaa                         <  2:680322/100‑1 (MQ=255)

                                                                               gCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATAtt                     >  1:2953528/1‑100 (MQ=255)

                                                                                cATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTc                    >  1:2994124/1‑100 (MQ=255)

                                                                                  tAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCgg                  >  1:2756799/1‑100 (MQ=255)

                                                                                   aaaaaTGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGc                 <  2:1151059/100‑1 (MQ=255)

                                                                                    aaaaTGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCg                >  1:173253/1‑100 (MQ=255)

                                                                                    aaaaTGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCg                >  1:524085/1‑100 (MQ=255)

                                                                                    aaaaTGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCg                >  1:2786382/1‑100 (MQ=255)

                                                                                     aaaTGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGc                 >  2:553927/1‑98 (MQ=255)

                                                                                     aaaTGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGt               >  2:2651583/1‑100 (MQ=255)

                                                                                        tGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTccc            >  1:145398/1‑100 (MQ=255)

                                                                                         ggTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCg           >  1:2234278/1‑100 (MQ=255)

                                                                                          gTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGAGTCCCgg          >  1:849071/1‑100 (MQ=255)

                                                                                          gTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCgg          <  1:1473998/100‑1 (MQ=255)

                                                                                          gTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCgg          >  2:1870455/1‑100 (MQ=255)

                                                                                          gTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCgg          <  1:585154/100‑1 (MQ=255)

                                                                                            tGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGtt        <  2:1395382/100‑1 (MQ=255)

                                                                                             gACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGttt       <  1:110779/100‑1 (MQ=255)

                                                                                              aCATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTc      <  1:2988081/100‑1 (MQ=255)

                                                                                              aCATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTc      <  2:595237/100‑1 (MQ=255)

                                                                                                 tAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAg   <  2:133004/100‑1 (MQ=255)

                                                                                                  aaaCTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGc  <  2:456012/100‑1 (MQ=255)

TGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGC  >  NC_000913/1580030‑1580227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A