Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F133 I1 R1	102	83.9	4092546	96.1%	3932936	99.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,485,264	G→A	W297* (TGG→TGA)	evgS →	hybrid sensory histidine kinase in two‑component regulatory system with EvgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,485,264	0	G	A	99.0%	349.0 / ‑4.4	99	W297* (TGG→TGA)	evgS	hybrid sensory histidine kinase in two‑component regulatory system with EvgA
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (53/45); total (54/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

ATGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGG  >  NC_000913/2485167‑2485359

aTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAAt                                                                                               <  1:238215/100‑1 (MQ=255)

 tGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAAtt                                                                                              <  2:1216144/100‑1 (MQ=255)

 tGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAAtt                                                                                              <  2:1913704/100‑1 (MQ=255)

  gAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTa                                                                                             <  1:342676/100‑1 (MQ=255)

  gAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTa                                                                                             >  1:1829429/1‑100 (MQ=255)

  gAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACAGGAAAAACAGTGAATTa                                                                                             >  1:641243/1‑100 (MQ=255)

      ttCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAagca                                                                                         <  1:163573/100‑1 (MQ=255)

        cGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAagcagc                                                                                       >  2:146476/1‑100 (MQ=255)

        cGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAagcagc                                                                                       >  1:2021611/1‑100 (MQ=255)

        cGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAagcagc                                                                                       <  2:642432/100‑1 (MQ=255)

         gCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAgcagca                                                                                      >  1:1003730/1‑100 (MQ=255)

         gCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAgcagca                                                                                      <  1:1144287/100‑1 (MQ=255)

          cTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGTCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCAt                                                                                     >  2:2024626/1‑100 (MQ=255)

            aTGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATcc                                                                                   >  1:641351/1‑100 (MQ=255)

                  tATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAAttt                                                                             <  2:1620995/100‑1 (MQ=255)

                   aTCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTa                                                                            >  1:618164/1‑100 (MQ=255)

                    tCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTaa                                                                           >  1:1367298/1‑100 (MQ=255)

                     cacaAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTaaa                                                                          <  2:1323230/100‑1 (MQ=255)

                     cacaAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTaaa                                                                          >  2:1778874/1‑100 (MQ=255)

                     cacaAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTaaa                                                                          <  1:2033549/100‑1 (MQ=255)

                     cacaAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTaaa                                                                          <  1:1900430/100‑1 (MQ=255)

                     cacaAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTaaa                                                                          >  1:1454314/1‑100 (MQ=255)

                     cacaAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTaaa                                                                          <  1:17621/100‑1 (MQ=255)

                      acaAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAg                                                                         >  1:994271/1‑100 (MQ=255)

                      acaAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAg                                                                         >  2:628022/1‑100 (MQ=255)

                      acaAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAg                                                                         >  2:1319112/1‑100 (MQ=255)

                      acaAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAg                                                                         >  1:903601/1‑100 (MQ=255)

                      acaAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAg                                                                         >  1:399244/1‑100 (MQ=255)

                      acaAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAg                                                                         >  2:730271/1‑100 (MQ=255)

                       caAAATTGGCTTGATACAGGAATCCTGGCCTTTCTGAGCAAACCATTCGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAgg                                                                        <  2:1375464/100‑1 (MQ=255)

                       caAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAgg                                                                        <  2:1878918/100‑1 (MQ=255)

                       caAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAgg                                                                        <  2:43683/100‑1 (MQ=255)

                        aaaaTTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGt                                                                       <  1:395209/100‑1 (MQ=255)

                           aTTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCt                                                                    <  2:218961/100‑1 (MQ=255)

                             ctgCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTgg                                                                  <  1:323417/98‑1 (MQ=255)

                               gCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGag                                                                >  2:323416/1‑99 (MQ=255)

                                  tGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAAt                                                             >  2:1672577/1‑100 (MQ=255)

                                     tACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCt                                                          >  1:1233723/1‑100 (MQ=255)

                                     tACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCt                                                          >  1:1204926/1‑100 (MQ=255)

                                     tACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCt                                                          >  1:1764318/1‑100 (MQ=255)

                                       cAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTa                                                        >  1:361906/1‑100 (MQ=255)

                                        aGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTAc                                                       <  2:11033/100‑1 (MQ=255)

                                        aGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTAc                                                       >  1:934545/1‑100 (MQ=255)

                                        aGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTAc                                                       >  1:43179/1‑100 (MQ=255)

                                        aGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTAc                                                       <  1:1241350/100‑1 (MQ=255)

                                         ggAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACt                                                      >  2:359197/1‑100 (MQ=255)

                                         ggAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACt                                                      <  1:1672590/100‑1 (MQ=255)

                                         ggAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACt                                                      >  1:1282714/1‑100 (MQ=255)

                                               cTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGcccc                                                <  2:48333/100‑1 (MQ=255)

                                                 ggCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGcccccc                                              <  2:1211419/100‑1 (MQ=255)

                                                   ccTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTa                                            >  1:1453078/1‑100 (MQ=255)

                                                   ccTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTa                                            >  2:918625/1‑100 (MQ=255)

                                                    cTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTAt                                           <  1:46456/100‑1 (MQ=255)

                                                     tttCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTAtt                                          >  2:1551655/1‑100 (MQ=255)

                                                        cTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTa                                       >  2:984580/1‑100 (MQ=255)

                                                        cTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTa                                       >  1:1659778/1‑100 (MQ=255)

                                                        cTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTa                                       <  1:1319120/100‑1 (MQ=255)

                                                         tGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTAt                                      >  1:1047681/1‑100 (MQ=255)

                                                         tGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTAt                                      >  1:812533/1‑100 (MQ=255)

                                                          gAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATg                                     <  2:1003725/100‑1 (MQ=255)

                                                          gAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATg                                     >  2:904449/1‑100 (MQ=255)

                                                          gAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATg                                     >  2:776617/1‑100 (MQ=255)

                                                           atctaaCCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGa                                    <  2:1238631/96‑1 (MQ=255)

                                                           aaCAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGa                                    <  2:1651150/100‑1 (MQ=255)

                                                              aaaCCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACgg                                 >  2:1988924/1‑100 (MQ=255)

                                                               aaCCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGaagat                                >  1:1238637/1‑96 (MQ=255)

                                                                aCCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGAt                               <  1:559116/100‑1 (MQ=255)

                                                                aCCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGAt                               <  1:422450/100‑1 (MQ=255)

                                                                aCCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGAt                               <  2:1953622/100‑1 (MQ=255)

                                                                 ccATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATg                              <  2:812530/100‑1 (MQ=255)

                                                                 ccATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATg                              <  1:1036941/100‑1 (MQ=255)

                                                                   aTTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGaa                            >  2:1347810/1‑100 (MQ=255)

                                                                      aGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAAt                         <  1:896619/100‑1 (MQ=255)

                                                                       gAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATg                        <  2:540845/100‑1 (MQ=255)

                                                                       gAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATg                        <  1:1778889/100‑1 (MQ=255)

                                                                        aaCTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATgg                       >  2:277635/1‑100 (MQ=255)

                                                                         aCTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGc                      <  2:295079/100‑1 (MQ=255)

                                                                         aCTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGc                      >  1:532691/1‑100 (MQ=255)

                                                                         aCTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGc                      <  2:641349/100‑1 (MQ=255)

                                                                          cTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCt                     >  2:559618/1‑100 (MQ=255)

                                                                           tCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTc                    >  2:41289/1‑100 (MQ=255)

                                                                            cACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCg                   >  1:528330/1‑100 (MQ=255)

                                                                            cACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCg                   >  1:1641251/1‑100 (MQ=255)

                                                                                  aCATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAATTGGCTCGGTTCgg             <  2:1264883/100‑1 (MQ=255)

                                                                                   cATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCggg            <  1:498040/100‑1 (MQ=255)

                                                                                    aTGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCgggg           <  2:1047676/100‑1 (MQ=255)

                                                                                    aTGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCgggg           <  1:918629/100‑1 (MQ=255)

                                                                                     tGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGc          <  2:361905/100‑1 (MQ=255)

                                                                                     tGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGc          >  2:1468360/1‑100 (MQ=255)

                                                                                     tGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGc          >  1:207095/1‑100 (MQ=255)

                                                                                     tGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGc          >  2:90506/1‑100 (MQ=255)

                                                                                       aaaaaCAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGt        >  1:255786/1‑100 (MQ=255)

                                                                                        aaaaCAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGtt       <  2:1909389/100‑1 (MQ=255)

                                                                                        aaaaCAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGtt       >  1:1299249/1‑100 (MQ=255)

                                                                                        aaaaCAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGt        >  2:729262/1‑99 (MQ=255)

                                                                                           aCAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATg    >  2:1964992/1‑100 (MQ=255)

                                                                                            cAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATgg   >  2:260832/1‑100 (MQ=255)

                                                                                             aGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATggg  <  2:618162/100‑1 (MQ=255)

                                                                                             aGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATggg  >  1:255501/1‑100 (MQ=255)

ATGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGG  >  NC_000913/2485167‑2485359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A