Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F204 I0 R1	247	174.3	8556966	94.2%	8060661	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,485,264	G→A	47.6%	W297* (TGG→TGA)	evgS →	hybrid sensory histidine kinase in two‑component regulatory system with EvgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,485,264	0	G	A	47.6%	29.1 / 214.6	162	W297* (TGG→TGA)	evgS	hybrid sensory histidine kinase in two‑component regulatory system with EvgA
Reads supporting (aligned to +/- strand): ref base G (40/45); new base A (39/38); total (79/83)
Fisher's exact test for biased strand distribution p-value = 7.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.04e-01

Only 100 of 167 total aligned reads displayed.

AAATGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGAC  >  NC_000913/2485165‑2485363

  aTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGAt                                                                                                   <  1:3812761/100‑1 (MQ=255)

  aTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGAt                                                                                                   <  2:2000393/100‑1 (MQ=255)

  aTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAAt                                                                                                   >  1:1683239/1‑100 (MQ=255)

    gAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTa                                                                                                 <  1:4234692/100‑1 (MQ=255)

       gTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAagc                                                                                              >  1:4126038/1‑100 (MQ=255)

       gTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAagc                                                                                              >  1:2548193/1‑100 (MQ=255)

       gTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAagc                                                                                              <  2:1490019/100‑1 (MQ=255)

       gTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAagc                                                                                              <  1:3068810/100‑1 (MQ=255)

         tagatATGAAGTATCACAAAATTGGCTTAATACAGGAAACCTGGCATTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAagca                                                                                             <  1:1109727/95‑1 (MQ=255)

         tCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAagcag                                                                                            <  2:2520303/100‑1 (MQ=255)

          cGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAagcagc                                                                                           <  2:2066771/100‑1 (MQ=255)

           gCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAgcagca                                                                                          >  1:2040024/1‑100 (MQ=255)

            cTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCAt                                                                                         >  1:4034134/1‑100 (MQ=255)

            cTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCAt                                                                                         >  1:1129126/1‑100 (MQ=255)

              aTGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATcc                                                                                       >  2:3841159/1‑100 (MQ=255)

              aTGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATCAAGCAGCATcc                                                                                       <  2:2562836/100‑1 (MQ=255)

               tGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATccc                                                                                      <  1:2098216/100‑1 (MQ=255)

                gAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCa                                                                                     >  1:4105111/1‑100 (MQ=255)

                  aGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAAt                                                                                   >  2:503210/1‑100 (MQ=255)

                   gTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAAtt                                                                                  <  2:3583606/100‑1 (MQ=255)

                    tATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAAttt                                                                                 >  1:753342/1‑100 (MQ=255)

                        acaAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAg                                                                             <  2:3380331/100‑1 (MQ=255)

                        acaAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTaa                                                                               >  2:4256543/1‑98 (MQ=255)

                         caAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAgg                                                                            <  2:2719824/100‑1 (MQ=255)

                         caAAATTGGCTTGAGACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAgg                                                                            <  2:787264/100‑1 (MQ=255)

                              ttGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTg                                                                       <  1:1959604/100‑1 (MQ=255)

                               tGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTgg                                                                      <  1:2374938/100‑1 (MQ=255)

                                ggCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGa                                                                     <  2:2576170/100‑1 (MQ=255)

                                 gattGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGaa                                                                    >  2:2901870/3‑100 (MQ=255)

                                  cTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGaaa                                                                   >  2:699822/1‑100 (MQ=255)

                                    tGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAAt                                                                 >  2:3503375/1‑100 (MQ=255)

                                    tGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGAGCTGGAAAAt                                                                 >  1:2937253/1‑100 (MQ=255)

                                     gATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAATCGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGaaaa                                                                  >  1:4022212/1‑98 (MQ=255)

                                     gATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATc                                                                <  2:3805952/100‑1 (MQ=255)

                                     gATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATc                                                                >  2:874712/1‑100 (MQ=255)

                                     gATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATc                                                                <  2:1721770/100‑1 (MQ=255)

                                      aTACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATcc                                                               <  1:620117/100‑1 (MQ=255)

                                       tACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCt                                                              <  1:439352/100‑1 (MQ=255)

                                         cAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTa                                                            >  2:542068/1‑100 (MQ=255)

                                         cAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTa                                                            >  1:736570/1‑100 (MQ=255)

                                         cAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTa                                                            >  1:2975652/1‑100 (MQ=255)

                                           ggAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACt                                                          <  1:3602401/100‑1 (MQ=255)

                                           ggAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACt                                                          >  2:2463786/1‑100 (MQ=255)

                                           ggAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACt                                                          >  1:3584619/1‑100 (MQ=255)

                                            gAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTc                                                         >  2:2772004/1‑100 (MQ=255)

                                              aaCCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGc                                                       >  2:3300294/1‑100 (MQ=255)

                                               aCCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGcc                                                      <  1:813896/100‑1 (MQ=255)

                                                ccTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGccc                                                     >  1:549090/1‑100 (MQ=255)

                                                ccTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGccc                                                     >  2:1564423/1‑100 (MQ=255)

                                                ccTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGccc                                                     <  1:2442349/100‑1 (MQ=255)

                                                 cTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGcccc                                                    <  1:2840172/100‑1 (MQ=255)

                                                  tGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAGAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGccccc                                                   >  1:823740/1‑100 (MQ=255)

                                                  tGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGccccc                                                   <  2:3044707/100‑1 (MQ=255)

                                                     ccTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTa                                                >  1:1332129/1‑100 (MQ=255)

                                                        ttCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTc                                             <  2:3973895/100‑1 (MQ=255)

                                                        ttCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTc                                             <  1:3412565/100‑1 (MQ=255)

                                                        ttCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTc                                             <  2:1257785/100‑1 (MQ=255)

                                                          cTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTa                                           <  2:2639392/100‑1 (MQ=255)

                                                            gAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATg                                         >  1:216117/1‑100 (MQ=255)

                                                            gAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATCTCAAGATCCTGTAACATCCTTACTCCCCCCCCTATTc                                             >  1:909686/1‑96 (MQ=255)

                                                             aaCAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGa                                        >  1:3254976/1‑100 (MQ=255)

                                                              aCAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGAc                                       >  2:704644/1‑100 (MQ=255)

                                                              aCAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCATACTCGCCCCCCTATTCTATGAc                                       <  1:4118442/100‑1 (MQ=255)

                                                              aCAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGAc                                       <  2:1757099/100‑1 (MQ=255)

                                                              aCAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGAc                                       >  2:2448316/1‑100 (MQ=255)

                                                                aaaCCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCCGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACgg                                     <  1:1292259/100‑1 (MQ=255)

                                                                aaaCCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACgg                                     >  2:1554328/1‑100 (MQ=255)

                                                                aaaCCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACgg                                     <  2:3611310/100‑1 (MQ=255)

                                                                  nccATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGAt                                   >  1:1532756/2‑100 (MQ=255)

                                                                    ccttAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGa                                 >  2:2263656/3‑100 (MQ=255)

                                                                    cATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATg                                  <  1:15377/99‑1 (MQ=255)

                                                                      ttAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGaaa                               >  2:2406716/1‑100 (MQ=255)

                                                                          aaCTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATgg                           <  1:914062/100‑1 (MQ=255)

                                                                           aCTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGc                          >  2:1543008/1‑100 (MQ=255)

                                                                              cACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCg                       >  2:4002398/1‑100 (MQ=255)

                                                                              cACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCg                       >  2:1091893/1‑100 (MQ=255)

                                                                               aCTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCgg                      <  2:3700648/100‑1 (MQ=255)

                                                                               aCTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCgg                      <  1:3102045/100‑1 (MQ=255)

                                                                               aCTGAACATGAAAAACAGGGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCATTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCgg                      <  1:3653151/100‑1 (MQ=255)

                                                                                   aaCATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCg                  <  1:773845/100‑1 (MQ=255)

                                                                                     cATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCggg                >  1:101496/1‑100 (MQ=255)

                                                                                     cATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCggg                <  2:1707771/100‑1 (MQ=255)

                                                                                     cATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCggg                <  1:2933295/100‑1 (MQ=255)

                                                                                     cATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCggg                >  1:433404/1‑100 (MQ=255)

                                                                                      aTGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCgggg               <  1:2771993/100‑1 (MQ=255)

                                                                                       tGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCggg                >  1:614616/1‑98 (MQ=255)

                                                                                       tGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGc              >  2:3395049/1‑100 (MQ=255)

                                                                                       tGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGc              >  2:1060534/1‑100 (MQ=255)

                                                                                       tGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGc              >  2:61051/1‑100 (MQ=255)

                                                                                              cAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATgg       >  2:1516300/1‑100 (MQ=255)

                                                                                               aGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATggg      <  1:1031564/100‑1 (MQ=255)

                                                                                               aGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATggg      >  2:2191536/1‑100 (MQ=255)

                                                                                               aGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATggg      <  2:1990425/100‑1 (MQ=255)

                                                                                               aGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATggg      <  2:3142143/100‑1 (MQ=255)

                                                                                               aGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATggg      <  1:1777191/100‑1 (MQ=255)

                                                                                                gTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACTGATGAAAATGGCTCGGTTCGGGGCGTTATgggg     <  2:133054/100‑1 (MQ=255)

                                                                                                gTGAATTAAGCAGCATCCCAATTTAAAGGTGCGGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATgggg     <  1:2703163/100‑1 (MQ=255)

                                                                                                 tGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATggggg    <  1:3043147/100‑1 (MQ=255)

                                                                                                  ggATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATgggg     <  2:3824095/98‑1 (MQ=255)

                                                                                                   gATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGAc  >  1:1774446/1‑100 (MQ=255)

AAATGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGAC  >  NC_000913/2485165‑2485363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A