Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F133 I1 R1
|
102 |
83.9 |
4092546 |
96.1% |
3932936 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,521,967 |
C→A |
A171S (GCG→TCG) |
xapR ← |
transcriptional activator of xapAB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,521,967 | 0 | C | A | 87.5%
| 179.4
/ 21.0
| 65 | A171S (GCG→TCG) | xapR | transcriptional activator of xapAB |
| Reads supporting (aligned to +/- strand): ref base C (4/4); new base A (31/25); total (35/30) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.00e-01 |
GGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCA > NC_000913/2521870‑2522062
|
ggcggcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAacgacg < 1:1454786/100‑1 (MQ=255)
gcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTc > 1:497419/1‑100 (MQ=255)
gcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTc < 2:623139/100‑1 (MQ=255)
cATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCa > 2:1013917/1‑100 (MQ=255)
ttGTAACAAAATTCTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAAGCATCGCCACCAGAAACGACGATTCATg < 1:1963993/100‑1 (MQ=255)
ttGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATg < 1:1756678/100‑1 (MQ=255)
tGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGc < 2:78342/100‑1 (MQ=255)
tAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCaa > 2:1571858/1‑100 (MQ=255)
aaCAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGTCGATTCATGCagc < 1:1384955/100‑3 (MQ=255)
aCAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAAcc > 2:821875/1‑100 (MQ=255)
aCAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAAcc > 1:415636/1‑100 (MQ=255)
gTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTa > 2:123425/1‑100 (MQ=255)
tCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTaa > 2:1397914/1‑100 (MQ=255)
tCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTaa > 1:1778470/1‑100 (MQ=255)
cACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTaaa > 1:847082/1‑100 (MQ=255)
aCGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTaaaa > 1:1543918/1‑100 (MQ=255)
cGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAAc < 2:2041502/100‑1 (MQ=255)
cGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAAc > 1:586051/1‑100 (MQ=255)
cGTAGCGCGTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAAc < 1:1408961/100‑1 (MQ=255)
gTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAAcc < 2:1778455/100‑1 (MQ=255)
gTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAAcc < 1:1271189/100‑1 (MQ=255)
aGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAg < 2:497418/100‑1 (MQ=255)
aGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAg > 1:831553/1‑100 (MQ=255)
cgcTTCAAGCGGGATGGAGGATAATGATGAGAGATGCTGATCTTCAGGCAGCTCCACCAGAAACGACGATGCATTCAACCGGAAGCTGGTAAAACCAGTc > 2:885149/1‑100 (MQ=255)
gcTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCg > 2:426191/1‑100 (MQ=255)
cTTCCAGCGGGACGGTGGAAAATGATGAGAGATGGTGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCgg < 1:1546331/100‑1 (MQ=255)
cTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCgg < 1:1957102/100‑1 (MQ=255)
ttCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCggt < 1:1076853/100‑1 (MQ=255)
tCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCggtg < 2:299701/100‑1 (MQ=255)
ccAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCggtgg < 2:146300/100‑1 (MQ=255)
cAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCggtggt < 1:1530447/100‑1 (MQ=255)
cGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGc > 1:1769384/1‑100 (MQ=255)
ggTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGcc < 1:1771706/100‑1 (MQ=255)
ggTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGcc < 1:1571868/100‑1 (MQ=255)
ggAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCAtt > 2:287646/1‑100 (MQ=255)
gAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTc > 1:1978251/1‑100 (MQ=255)
gAAAACGATGAGAGCTGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCAAGCAACCGTAAGCTGGTAAAACCAGCCGCTGGTTCTTTCGCCATTc > 2:144912/1‑100 (MQ=255)
aaaaTGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCg > 1:1366244/1‑100 (MQ=255)
atgatgAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCa < 1:1833442/100‑1 (MQ=255)
tgaGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATc > 2:1767357/1‑100 (MQ=255)
gagagaTGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATcc < 1:1013922/100‑1 (MQ=255)
gagagaTGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATcc < 2:1363811/100‑1 (MQ=255)
agagaTGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATccc > 1:1380529/1‑100 (MQ=255)
agagaTGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATccc > 2:1924662/1‑100 (MQ=255)
agaTGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCgg < 1:632384/100‑1 (MQ=255)
gatgatGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGc > 2:528904/1‑100 (MQ=255)
tgatgCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCAt > 1:1918181/1‑100 (MQ=255)
gatgCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATc < 1:990931/100‑1 (MQ=255)
tgCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCaa < 2:415635/100‑1 (MQ=255)
gCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAg < 2:586049/100‑1 (MQ=255)
ctTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTc > 2:174353/1‑100 (MQ=255)
tCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTcgc > 2:741717/1‑100 (MQ=255)
tCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTcgc > 2:1833194/1‑100 (MQ=255)
cAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTcgcg > 2:1292396/1‑100 (MQ=255)
ggCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcggc < 2:2025604/100‑1 (MQ=255)
ggCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcggc > 1:2025622/1‑100 (MQ=255)
cATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcggc < 1:885152/98‑1 (MQ=255)
gCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTc < 2:692609/98‑1 (MQ=255)
ccaccaGACACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCCGATCCCGGCATCCAGTTCGCGGCGTTCCAg > 2:1749691/1‑100 (MQ=255)
caccaGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGc > 2:1717166/1‑100 (MQ=255)
accaGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCa < 1:1767371/100‑1 (MQ=255)
accaGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCa > 2:186664/1‑100 (MQ=255)
ccaGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCaa > 1:1768817/1‑100 (MQ=255)
caGAAACGACGATTCATGCAACCGTAAGCTTGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAg < 2:239933/100‑1 (MQ=255)
gAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGc > 2:1619844/1‑100 (MQ=255)
aaCGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCa > 1:1501096/1‑100 (MQ=255)
|
GGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCA > NC_000913/2521870‑2522062
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
