Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F133 I1 R1
|
102 |
83.9 |
4092546 |
96.1% |
3932936 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,574,062 |
A→T |
C273S (TGC→AGC) |
asd ← |
aspartate‑semialdehyde dehydrogenase, NAD(P)‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,574,062 | 0 | A | T | 97.5%
| 274.7
/ ‑1.8
| 79 | C273S (TGC→AGC) | asd | aspartate‑semialdehyde dehydrogenase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base T (35/42); total (37/42) |
| Fisher's exact test for biased strand distribution p-value = 2.16e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 |
ACGACTTTCGCCCACGGATTGTGCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCAGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTCCGCCTGCCCTTTCCACTCT > NC_000913/3573965‑3574156
|
cttccgatctcccACGGATTGTGCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTgc < 1:1915631/90‑1 (MQ=255)
cGACTTTCGCCCACGGATTGTGCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTgcg < 2:693718/100‑1 (MQ=255)
gACTTTCGCCCACGGATTGTGCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTgcgc < 2:882065/100‑1 (MQ=255)
aCTTTCGCCCACGGATTGTGCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCa < 1:282653/100‑1 (MQ=255)
cTTTCGCCCACGGATTGTGCGCAGCCAGCAGTTATTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCaa > 2:1328990/1‑100 (MQ=255)
gCCCACGGATTGTGCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGccc > 1:1794012/1‑100 (MQ=255)
gCCCACGGATTGTGCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGccc > 1:1509228/1‑100 (MQ=255)
gCCCACGGATTGTGCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGccc < 1:1611342/100‑1 (MQ=255)
cccACGGATTGTGCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTgcagatcggaag > 2:1915613/1‑90 (MQ=255)
ccACGGATTGTGCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCg < 2:1042780/100‑1 (MQ=255)
ccACGGATTGTGCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCg < 2:1427042/100‑1 (MQ=255)
cACGGATTGTGCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGTATGCCTGGCTGTGGCTGCGCAATGCCCCGa > 2:2020880/1‑100 (MQ=255)
aCGGATTGTGCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGac < 2:1280728/100‑1 (MQ=255)
gATTGTGCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACg > 1:278730/1‑100 (MQ=255)
ttGTGCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGca < 2:295577/100‑1 (MQ=255)
ttGTGCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGca < 1:1276287/100‑1 (MQ=255)
gtgCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGcaca < 1:1931004/100‑1 (MQ=255)
gcgcAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGcacaca > 1:293053/1‑100 (MQ=255)
cgcAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACAt > 1:688350/1‑100 (MQ=255)
gcAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTTTTTTTATTATTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATa > 2:1093466/1‑100 (MQ=255)
gcAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATa < 2:234006/100‑1 (MQ=255)
gccagcAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAAc > 2:1675086/1‑100 (MQ=255)
gcagTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATc > 2:623551/1‑100 (MQ=255)
gTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTAc > 1:1711773/1‑100 (MQ=255)
ttcttcCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTAcc < 2:1742421/100‑1 (MQ=255)
ttcttcCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTAcc < 1:672573/100‑1 (MQ=255)
tcttcCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCg > 1:1044991/1‑100 (MQ=255)
tcttcCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCg < 2:35374/100‑1 (MQ=255)
cttcCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCgg > 2:1536848/1‑100 (MQ=255)
tcCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGaa > 1:815784/1‑100 (MQ=255)
ccACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGaa > 2:1204860/1‑99 (MQ=255)
aCGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTa < 1:1243671/100‑1 (MQ=255)
ggTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCAGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAAttcc > 2:1708173/1‑97 (MQ=255)
cGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGaa < 2:497075/100‑1 (MQ=255)
cGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGaa < 2:497390/100‑1 (MQ=255)
ggAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAg > 1:124721/1‑100 (MQ=255)
gAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAg > 2:977002/1‑99 (MQ=255)
aaTAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGAt > 2:1148796/1‑100 (MQ=255)
aGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGAtgtg > 2:411032/1‑100 (MQ=255)
aGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGAtgtg > 2:462744/1‑100 (MQ=255)
gACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCAGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATgtgt > 1:442079/1‑100 (MQ=255)
tcttccgatctttCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGtt < 2:1771058/90‑1 (MQ=255)
caTCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAg > 1:697206/1‑100 (MQ=255)
aTCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAgg < 2:876377/100‑1 (MQ=255)
aTCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAgg < 1:1186176/100‑1 (MQ=255)
tCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTTCCGTAATTCCGGTAGAtggtttatgtg < 1:1328999/100‑10 (MQ=25)
tCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGa < 2:1509219/100‑1 (MQ=255)
ttttttttttttATTAGTGAATGCCTGGCTGTGGCTGCGCAATGCCTCGACACGCACACATAAACCATCTACCGGAATTACGGATGTTGTTTTTAGGAt > 2:1855463/10‑99 (MQ=255)
ttttttCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATc < 2:278729/100‑1 (MQ=255)
tttttCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCt < 2:169161/100‑1 (MQ=255)
tttttCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCt < 1:1597994/100‑1 (MQ=255)
tttttCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCt < 2:293052/100‑1 (MQ=255)
tttttCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCt < 1:1711956/100‑1 (MQ=255)
tttttCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCt < 2:483311/100‑1 (MQ=255)
tttttCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCt < 2:773612/100‑1 (MQ=255)
tttttCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGAATCt < 2:508558/100‑1 (MQ=255)
tCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCttgtt < 2:1044986/100‑1 (MQ=255)
tCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCttgtt < 1:1148802/100‑1 (MQ=255)
cAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCttgttg > 2:133797/1‑100 (MQ=255)
cAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCttgttg > 1:1139960/1‑100 (MQ=255)
cAATTTAAGAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCttgttg < 1:924307/100‑1 (MQ=255)
tttAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGtt < 1:1610235/100‑1 (MQ=255)
tttAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGtt > 1:1553245/1‑100 (MQ=255)
tAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTc > 2:1540487/1‑100 (MQ=255)
aaTAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTcc < 2:141928/100‑1 (MQ=255)
tAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCTGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTCCGc > 2:565684/1‑100 (MQ=255)
tAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTCCGc > 1:1258461/1‑100 (MQ=255)
aGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTCCGcc > 2:1203613/1‑100 (MQ=255)
aGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTCCGcc < 1:34770/100‑1 (MQ=255)
tGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTCCGCCTg < 2:502890/100‑1 (MQ=255)
tGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTCCGCCTg < 2:2027191/100‑1 (MQ=255)
gAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTCCGCCTGc > 2:814439/1‑100 (MQ=255)
cTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTCCGCCTGCCCTTTc < 1:623553/100‑1 (MQ=255)
tggctgtggctgCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTCCGCCTGCCCTTTcc < 2:1793996/100‑1 (MQ=255)
ggCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTCCGCCTGCCATTTCCa > 2:1124323/1‑100 (MQ=255)
gCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTCCGCCTGCCCTTTCCAc > 1:1295496/1‑100 (MQ=255)
gCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTCCGCCTGCCCTTTCCAc > 1:930400/1‑100 (MQ=255)
cTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGCAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTCCGCCTGCCCTTTCCAct > 2:1136618/1‑100 (MQ=255)
gtgGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTCCGCCTGCCCTTTCCActct < 2:815781/100‑1 (MQ=255)
gtgGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTCCGCCTGCCCTTTCCActct < 2:917812/100‑1 (MQ=255)
|
ACGACTTTCGCCCACGGATTGTGCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCAGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTCCGCCTGCCCTTTCCACTCT > NC_000913/3573965‑3574156
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
