Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F204 I1 R1
|
117 |
93.2 |
4682243 |
93.4% |
4373214 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,574,062 |
A→T |
C273S (TGC→AGC) |
asd ← |
aspartate‑semialdehyde dehydrogenase, NAD(P)‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,574,062 | 0 | A | T | 100.0%
| 193.7
/ NA
| 55 | C273S (TGC→AGC) | asd | aspartate‑semialdehyde dehydrogenase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (27/28); total (27/28) |
CGACTTTCGCCCACGGATTGTGCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCAGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTCCGCCTGCCCTTTCCACTC > NC_000913/3573966‑3574155
|
cGACTTTCGCCCACGGATTGTGCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTgcg < 2:1780400/100‑1 (MQ=255)
cTTTCGCCCACGGATTGTGCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTgcgc < 2:987127/98‑1 (MQ=255)
ttCGCCCACGGATTGTGCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATg < 1:1772689/100‑1 (MQ=255)
cccACGGATTGTGCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGcccc > 2:1352282/1‑100 (MQ=255)
cccACGGATTGTGCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAATGAATACCTGGCTATGGCTGCGCAATGcccc > 1:210185/1‑100 (MQ=255)
ccACGGATTGTGCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCg < 2:1850166/100‑1 (MQ=255)
ccACGGATTGTGCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCg < 1:2149851/100‑1 (MQ=255)
aCGGATTGTGCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGac > 2:1830179/1‑100 (MQ=255)
tgtgCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGcac < 2:485438/100‑1 (MQ=255)
gtgCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGcaca < 2:104046/100‑1 (MQ=255)
gtgCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGcaca > 1:2225590/1‑100 (MQ=255)
gtgCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGcaca > 1:817253/1‑100 (MQ=255)
gtgCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGcaca < 2:542349/100‑1 (MQ=255)
tgCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGcacac < 2:327633/100‑1 (MQ=255)
tgCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGcacac < 2:669680/100‑1 (MQ=255)
gccagcAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAAc > 2:2102109/1‑100 (MQ=255)
cagcagTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCa < 2:2106696/100‑1 (MQ=255)
agcagTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCAt > 2:1319468/1‑100 (MQ=255)
cagTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATc < 2:2129514/99‑1 (MQ=255)
gatcttcCACGGTAGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTAc > 1:1581749/3‑100 (MQ=255)
ttcttcCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTAcc > 2:1698028/1‑100 (MQ=255)
tcttcCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCg < 1:1420139/100‑1 (MQ=255)
cttcCACGGTCGGAATAGACACATCTTTTTTTTTTTTTTTTTTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCg > 2:552200/1‑99 (MQ=255)
cttcCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCgg > 1:2135681/1‑100 (MQ=255)
tcCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGaa > 2:1273639/1‑100 (MQ=255)
cACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAAtt > 1:492504/1‑100 (MQ=255)
gTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACgg < 2:846948/100‑1 (MQ=255)
gTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATATACCGGAATTAAgg > 1:2104877/1‑100 (MQ=255)
tCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGa < 1:1460968/100‑1 (MQ=255)
ggAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAg < 2:2225582/100‑1 (MQ=255)
ggAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAg < 2:860752/100‑1 (MQ=255)
aaTAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGAt < 2:1581744/100‑1 (MQ=255)
aTAGACACATCTTTTTTTTTTTTATTTGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATGACGGAAGAtg > 2:1529382/1‑100 (MQ=255)
aTAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGAtg > 1:1242904/1‑100 (MQ=255)
aTAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGAtg > 1:1242905/1‑100 (MQ=255)
aGACACATCTTTTTTTTTTTTATTTTTGAATGCCTGGCTGTGGCTACGCAATGCCCCGACACGCACACATAAATCATCTACCGGAATTACGGAAGAtgt > 2:2026329/1‑99 (MQ=255)
acacATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGtt < 1:552201/100‑1 (MQ=255)
cacaTCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTg < 2:439685/100‑1 (MQ=255)
tCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTTCCGTAATTCCGGTAGAtggtttatgtg < 1:2079540/100‑10 (MQ=25)
cTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGAt > 1:1375011/1‑100 (MQ=255)
cTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGAt > 2:629825/1‑100 (MQ=255)
tttttCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCt < 2:451399/100‑1 (MQ=255)
ttttCAATTTAATAGTGAATGCCGGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAGCCACCTACCGGAATGACGGAAGATGTGTTGAGGATCt < 2:1983029/99‑1 (MQ=255)
tttCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCttg < 1:1830184/100‑1 (MQ=255)
tCAATTTAATAGTGAATGTCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCttgtt < 1:2026336/100‑1 (MQ=255)
tCAATTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCttgtt > 2:747558/1‑100 (MQ=255)
aaTTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTgg < 1:2102116/100‑1 (MQ=255)
aTTTAATAGTGAATGCCTGGCTGTGGCTGCGCAATG‑CCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGtt < 2:803671/100‑1 (MQ=255)
aaTAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTcc > 2:1849954/1‑100 (MQ=255)
aTAGTGAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTCCg < 2:566905/100‑1 (MQ=255)
gAATGCCTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTCCGCCTGc > 2:2088673/1‑100 (MQ=255)
ccTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTCCGCCTGCCCttt > 1:1072342/1‑100 (MQ=255)
ccTGGCTGTGGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTCCCGCCTGCCCttt > 2:251671/1‑100 (MQ=255)
tggctgtggctgCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTCCGCCTGCCCTTTcc < 1:1849959/100‑1 (MQ=255)
tggctgtggctgCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTCCGCCTGCCCTTTcc < 1:1529386/100‑1 (MQ=255)
tggctgtggctgCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTCCGCCTGCCCTTTcc < 1:2191383/100‑1 (MQ=255)
tgtgGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTCCGCCTGCCCTTTCCActc > 1:928230/1‑100 (MQ=255)
tgtgGCTGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTCCGCCTGCCCTTTCCActc > 1:911313/1‑100 (MQ=255)
|
CGACTTTCGCCCACGGATTGTGCGCAGCCAGCAGTTCTTCCACGGTCGGAATAGACACATCTTTTTTCAATTTAATAGTGAATGCCTGGCTGTGGCAGCGCAATGCCCCGACACGCACACATAAACCATCTACCGGAATTACGGAAGATGTGTTGAGGATCTTGTTGGTTTCCGCCTGCCCTTTCCACTC > NC_000913/3573966‑3574155
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
