Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F133 I0 R1 200 171.4 8351354 94.3% 7875326 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 358,599 G→A 70.7% A31V (GCG→GTG)  cynR ← transcriptional activator of cyn operon; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913358,5990GA70.7% 202.3 / 117.4 153A31V (GCG→GTG) cynRtranscriptional activator of cyn operon; autorepressor
Reads supporting (aligned to +/- strand):  ref base G (26/19);  new base A (57/51);  total (83/70)
Fisher's exact test for biased strand distribution p-value = 5.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 155 total aligned reads displayed.

ACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCGAGAGAGCATTCGCAACC  >  NC_000913/358503‑358698
                                                                                                |                                                                                                   
aCTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAg                                                                                                  >  2:1103178/1‑100 (MQ=255)
  ttCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGt                                                                                                <  1:3398268/100‑1 (MQ=255)
   tctcCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGtt                                                                                               >  1:1548196/1‑100 (MQ=255)
    ctcCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTg                                                                                              >  1:2213145/1‑100 (MQ=255)
    ctcCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTg                                                                                              >  2:2268845/1‑100 (MQ=255)
     tcCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTgg                                                                                             >  1:1011021/1‑100 (MQ=255)
      ccTGCATAAGTGAGACGAATCGTTCGCCCGCTGCGGTCAAACAGCGGCACGACTAAACTCTCCTCTAACTGGCGACTCTGCTGGGAAAGCGCAGGTTggg                                                                                            >  2:1131262/1‑100 (MQ=255)
       cTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGga                                                                                           >  1:2140075/1‑100 (MQ=255)
        tGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGgag                                                                                          >  2:3111821/1‑100 (MQ=255)
         gCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGgaga                                                                                         >  2:816711/1‑100 (MQ=255)
           aTCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTATACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGgaga                                                                                         >  2:943188/1‑98 (MQ=255)
           aTCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACg                                                                                       >  1:275011/1‑100 (MQ=255)
            ncAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGt                                                                                      >  2:1960903/2‑100 (MQ=255)
              aGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGc                                                                                    >  1:711661/1‑100 (MQ=255)
               gTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCa                                                                                   >  2:2544682/1‑100 (MQ=255)
                tGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCaa                                                                                  >  1:405127/1‑100 (MQ=255)
                 gagaCGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAAc                                                                                 <  2:3235083/100‑1 (MQ=255)
                 gagaCGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAAc                                                                                 <  1:683075/100‑1 (MQ=255)
                   gaCGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGc                                                                               >  1:2980194/1‑100 (MQ=255)
                   gaCGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGc                                                                               >  2:2467415/1‑100 (MQ=255)
                   gaCGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGc                                                                               >  1:1345075/1‑100 (MQ=255)
                     cGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCAc                                                                             >  1:513186/1‑100 (MQ=255)
                      gAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACt                                                                            <  1:121607/100‑1 (MQ=255)
                       aaTCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTg                                                                           <  1:3927480/100‑1 (MQ=255)
                         tCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTggc                                                                         <  2:2388351/100‑1 (MQ=255)
                          cGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGATAGTACAGGTTGGGAGACGTGCAACGCACTggcg                                                                        <  2:2742065/100‑1 (MQ=255)
                           gTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTggcgg                                                                       >  1:1867178/1‑100 (MQ=255)
                           gTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACgagatcggaa                                                                       >  2:889841/1‑91 (MQ=255)
                            ttCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTggcggc                                                                      >  1:1211752/1‑100 (MQ=255)
                            ttCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTggcggc                                                                      <  2:1889418/100‑1 (MQ=255)
                               gCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACg                                                                   >  2:653021/1‑100 (MQ=255)
                               gCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACg                                                                   >  1:2821030/1‑100 (MQ=255)
                               gCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACg                                                                   >  2:1441725/1‑100 (MQ=255)
                                 ccGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACggg                                                                 <  1:2895731/100‑1 (MQ=255)
                                 ccGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACggg                                                                 <  1:2129335/100‑1 (MQ=255)
                                 ccGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACCGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACggg                                                                 <  1:1733347/100‑1 (MQ=255)
                                  cGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGt                                                                <  1:2361056/100‑1 (MQ=255)
                                    cTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGa                                                              >  1:2578090/1‑100 (MQ=255)
                                    cTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGa                                                              <  2:398551/100‑1 (MQ=255)
                                    cTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGa                                                              <  1:2488650/100‑1 (MQ=255)
                                      aCGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTg                                                               <  2:459899/97‑1 (MQ=255)
                                       cGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGc                                                           >  1:2850496/1‑100 (MQ=255)
                                           cAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGcc                                                       >  2:338516/1‑100 (MQ=255)
                                           cAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGcc                                                       <  1:2901964/100‑1 (MQ=255)
                                           cAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGcc                                                       >  2:3845742/1‑100 (MQ=255)
                                            aaaCAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCa                                                      <  1:4004924/100‑1 (MQ=255)
                                            aaaCAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCa                                                      <  2:3455566/100‑1 (MQ=255)
                                            aaaCAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCa                                                      <  1:2771720/100‑1 (MQ=255)
                                             aaCAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCa                                                      >  2:804981/1‑99 (MQ=255)
                                                 gCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTc                                                 <  1:2011176/100‑1 (MQ=255)
                                                 gCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTc                                                 <  2:1532854/100‑1 (MQ=255)
                                                  cGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCa                                                <  2:2013771/100‑1 (MQ=255)
                                                   ggCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAg                                               >  2:2354149/1‑100 (MQ=255)
                                                    gCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGc                                              <  1:455435/100‑1 (MQ=255)
                                                    gCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGc                                              <  2:275008/100‑1 (MQ=255)
                                                         ccTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGTGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACgg                                         <  1:3109677/100‑1 (MQ=255)
                                                         ccTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACgg                                         >  1:425635/1‑100 (MQ=255)
                                                         ccTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACgg                                         <  1:1441734/100‑1 (MQ=255)
                                                         ccTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACgg                                         >  1:3969840/1‑100 (MQ=255)
                                                           tccgatctcCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCa                                       <  1:3217686/95‑1 (MQ=255)
                                                            aaaCTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCaa                                      >  2:894847/1‑100 (MQ=255)
                                                             aaCTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAg                                     <  2:3752488/100‑1 (MQ=255)
                                                              aCTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGa                                    >  1:3570045/1‑100 (MQ=255)
                                                              aCTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGa                                    >  1:1822475/1‑100 (MQ=255)
                                                                  tcctcTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAaat                                >  1:1409931/1‑100 (MQ=255)
                                                                   cctcTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAaata                               >  1:521646/1‑100 (MQ=255)
                                                                   cctcTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAaata                               >  2:3231039/1‑100 (MQ=255)
                                                                    ctctAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAaata                               <  2:683685/99‑1 (MQ=255)
                                                                     tctAGCTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAaataa                              <  2:3165335/99‑1 (MQ=255)
                                                                     tctAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAaataat                             >  2:2279331/1‑100 (MQ=255)
                                                                      ctAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAAtt                            >  1:997640/1‑100 (MQ=255)
                                                                      ctAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAAtt                            <  2:1874271/100‑1 (MQ=255)
                                                                       tagcTGGCGCATGTGCTGGGAATGCGCAGGTTGGGAGGCGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAAtt                            <  1:3186882/96‑1 (MQ=255)
                                                                       tAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTg                           <  2:2950882/100‑1 (MQ=255)
                                                                          cTGGCGAATCTGCTGGGAAAGCGCAGGTTTGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGata                        <  1:888239/100‑1 (MQ=255)
                                                                           tGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAAACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGata                        <  1:3508189/99‑1 (MQ=255)
                                                                           tGGCGAATCTGCTGGGAAAGCACAGGGTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCACGAAAATAATTGata                        >  1:2545366/1‑99 (MQ=255)
                                                                            ggCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATg                      >  1:3707108/1‑100 (MQ=255)
                                                                             gCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGt                     <  1:1019472/100‑1 (MQ=255)
                                                                              cGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGt                     >  2:2594513/1‑99 (MQ=255)
                                                                              cGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTc                    >  1:2335332/1‑100 (MQ=255)
                                                                              cGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTc                    >  1:3382992/1‑100 (MQ=255)
                                                                                aaTCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCga                  <  1:1606834/100‑1 (MQ=255)
                                                                                   ctgctgGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCgagag               <  2:1113422/100‑1 (MQ=255)
                                                                                    tgctgGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCgagaga              >  1:1820199/1‑100 (MQ=255)
                                                                                     gctgGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCGagagag             <  2:3192143/100‑1 (MQ=255)
                                                                                     gctgGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCGagagag             <  2:2913714/100‑1 (MQ=255)
                                                                                      ctgGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCGAGAGAGc            >  1:2956684/1‑100 (MQ=255)
                                                                                      ctgGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCGAGAGAGc            <  2:212850/100‑1 (MQ=255)
                                                                                      ctgGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCGAGAGAGc            >  1:923032/1‑100 (MQ=255)
                                                                                        gggAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCGAGAGAGCAt          >  2:478303/1‑100 (MQ=255)
                                                                                        gggAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAAATGATATGTCGAGAGAGCAt          >  2:3403797/1‑100 (MQ=255)
                                                                                          gAAAGTACAGCTTGGCAGACGTGCAACTCACGGGCGGCACCGGTAAACCTTCCATGTTCATCCACGGCAAGAAAATAAGTGATATGTTCAGAGAGCATTc        <  2:3532512/100‑1 (MQ=255)
                                                                                          gAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCGAGAGAGCATTc        <  2:1573846/100‑1 (MQ=255)
                                                                                           aaaGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCGAGAGAGCATTCg       >  2:2082805/1‑100 (MQ=255)
                                                                                           aaaGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCGAGAGAGCATTCg       <  1:2386893/100‑1 (MQ=255)
                                                                                             aGCGCAGGTTAGGAGACGTGCAAAGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCGAGAGAGCATTCGCa     <  2:3701145/100‑1 (MQ=255)
                                                                                             aGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCGAGAGAGCATTCGCa     >  1:1723563/1‑100 (MQ=255)
                                                                                              gcgcAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCGAGAGAGCATTCGCaa    <  1:912685/100‑1 (MQ=255)
                                                                                                gcAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCGAGAGAGCATTCGCAAcc  <  2:362271/100‑1 (MQ=255)
                                                                                                |                                                                                                   
ACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCGAGAGAGCATTCGCAACC  >  NC_000913/358503‑358698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A