Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F133 I0 R1 200 171.4 8351354 94.3% 7875326 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,827,362 A→G 78.1% M1M (ATG→GTG) † srlB → glucitol/sorbitol‑specific enzyme IIA component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,827,3620AG78.1% 290.0 / 75.6 151M1M (ATG→GTG) †srlBglucitol/sorbitol‑specific enzyme IIA component of PTS
Reads supporting (aligned to +/- strand):  ref base A (15/18);  new base G (65/53);  total (80/71)
Fisher's exact test for biased strand distribution p-value = 4.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.14e-01

Only 100 of 151 total aligned reads displayed.

TCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCG  >  NC_000913/2827266‑2827457
                                                                                                |                                                                                               
tCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGa                                                                                              >  1:749538/1‑100 (MQ=255)
 cccTTCTGTACTGGTGAGCCGCTTTTTAACCTGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTg                                                                                               >  2:2992435/1‑98 (MQ=255)
 cccTTCTGTACTGGTGAGCCGCTTTTTAACCTGCGCGCCGACAGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGAc                                                                                             >  1:2817241/1‑100 (MQ=255)
 cccTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATCGAGGCTGAAACGTGAc                                                                                             >  2:1977576/1‑100 (MQ=255)
 cccTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGAc                                                                                             >  1:2862864/1‑100 (MQ=255)
 cccTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGAc                                                                                             <  2:890118/100‑1 (MQ=255)
 cccTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGAc                                                                                             <  1:105667/100‑1 (MQ=255)
 cccTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGAc                                                                                             <  1:1109838/100‑1 (MQ=255)
  ccTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGAcc                                                                                            >  2:387015/1‑100 (MQ=255)
   cTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCg                                                                                           >  1:3875199/1‑100 (MQ=255)
   cTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCg                                                                                           <  1:3584497/100‑1 (MQ=255)
     tCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGtt                                                                                         <  2:2107110/100‑1 (MQ=255)
     tCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGtt                                                                                         <  1:3696399/100‑1 (MQ=255)
       tGTCCTGGTGAGCCGCTTTTTAACCGGCGCGCCGCCTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGttat                                                                                       <  2:924454/100‑1 (MQ=255)
        gTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGttatt                                                                                      >  2:3214467/1‑100 (MQ=255)
        gTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGttatt                                                                                      >  1:3231568/1‑100 (MQ=255)
         tACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGttattt                                                                                     <  2:1081701/100‑1 (MQ=255)
          aCTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGttattta                                                                                    <  2:98630/100‑1 (MQ=255)
           cTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGttatttat                                                                                   >  2:3724095/1‑100 (MQ=255)
            tGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATc                                                                                  <  1:2004421/100‑1 (MQ=255)
             ggTGAGCCGCTTTTTAACCGGCGCGCCGCCTGTCCTGATCGCCTGGTTTATCTCCGGTCTTCTCTATCACTACACCCTGAATCGTAACCGTTATTtctc                                                                                  >  2:3934816/1‑99 (MQ=255)
             ggTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATc                                                                                  <  2:1361527/99‑1 (MQ=255)
             ggTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCa                                                                                 >  2:2275331/1‑100 (MQ=255)
             ggTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCa                                                                                 >  2:1349452/1‑100 (MQ=255)
              gTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAg                                                                                <  2:2325202/100‑1 (MQ=255)
                 aGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGac                                                                              >  2:2692129/1‑99 (MQ=255)
                  gCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGacca                                                                            <  2:2041137/100‑1 (MQ=255)
                  gCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGacca                                                                            >  2:2943347/1‑100 (MQ=255)
                    cGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGaccacc                                                                          >  1:2236367/1‑100 (MQ=255)
                     ggtttttAACCGGAGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGAccacca                                                                         >  1:2831953/3‑100 (MQ=255)
                     gCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGAccacca                                                                         >  2:3192158/1‑100 (MQ=255)
                      cgttttAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCAt                                                                        <  2:2527346/98‑1 (MQ=255)
                      cTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCAt                                                                        >  1:704788/1‑100 (MQ=255)
                        ttttAGCCGGCGCGCCGACTGTAGTGAGCGCCTGGTTGGTCTCTGGTTTTATCTATCGCTAGAGCCTGACAGGTGACCGTTATTTCTCAGAccgcca                                                                         >  2:1175444/1‑97 (MQ=255)
                        ttttAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCa                                                                      >  1:3603625/1‑100 (MQ=255)
                        ccgatctcGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCa                                                                      <  1:3087026/93‑1 (MQ=255)
                          ttAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCAcc                                                                    <  1:3849992/100‑1 (MQ=255)
                          ttAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCAcc                                                                    >  1:1952279/1‑100 (MQ=255)
                           tAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCCCCGGGTTTATCTATCAATAGGGGCTGAAACGGGGCCGTTATTTATTCGACCACCATCAccc                                                                   >  2:3786849/1‑100 (MQ=255)
                            aaCCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCg                                                                  <  2:2130680/100‑1 (MQ=255)
                               cGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTAACAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTAt                                                               >  1:8754/1‑100 (MQ=255)
                                  cgcgcCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTAGCAATAGAGGCTGAAACGTGACCGTTATATATCAGACCACCATCACCCGTATCgg                                                            >  1:3348652/1‑100 (MQ=255)
                                     gcCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGgcgc                                                         >  1:3541892/1‑100 (MQ=255)
                                     gcCGACTGTACTAATCGCCTGTTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGATCGTTATTTATCAGACCACCATCACCCGTAGCGgcgc                                                         <  2:3644216/100‑1 (MQ=255)
                                      ccGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCCATAGAGGCTGAAACATGACCGTTATTTATCAGCCCTCCATCACCCGTATCGGcgcg                                                        <  2:924567/100‑1 (MQ=255)
                                      ccGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGggcg                                                        <  2:4093128/100‑1 (MQ=255)
                                      ccGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGcgcg                                                        <  1:3526302/100‑1 (MQ=255)
                                       cGACTGTACTGATCGCCTGGTTTGTCTCCTGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGTCCACCATCACCCGTATCGGcgcg                                                        <  2:1840576/99‑1 (MQ=255)
                                          cTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTg                                                    <  2:1047603/100‑1 (MQ=255)
                                          cTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTg                                                    <  1:3366552/100‑1 (MQ=255)
                                          cTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTg                                                    >  1:1470103/1‑100 (MQ=255)
                                              aCTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCAt                                                >  2:1651701/1‑100 (MQ=255)
                                               cTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCAtt                                               >  2:1363765/1‑100 (MQ=255)
                                               cTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCAtt                                               <  2:2115000/100‑1 (MQ=255)
                                                tGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTg                                              >  1:1864246/1‑100 (MQ=255)
                                                tGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTg                                              <  2:1719347/100‑1 (MQ=255)
                                                 gATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTTTCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTg                                              >  2:3351503/1‑99 (MQ=255)
                                                 gATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGa                                             >  1:212587/1‑100 (MQ=255)
                                                 gATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGa                                             <  2:1079489/100‑1 (MQ=255)
                                                  aTCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGAc                                            <  2:438987/100‑1 (MQ=255)
                                                    cGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGc                                          <  1:141028/100‑1 (MQ=255)
                                                    cGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGc                                          <  1:1513696/100‑1 (MQ=255)
                                                     gCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGcc                                         >  1:3472262/1‑100 (MQ=255)
                                                        tGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTc                                      <  1:414645/100‑1 (MQ=255)
                                                          gTTTGTGTCCGGTTTTATCTATCAATAGGGGCTGAAGCGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAg                                    <  2:2284807/100‑1 (MQ=255)
                                                          gTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAg                                    <  1:3754809/100‑1 (MQ=255)
                                                          gTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAg                                    <  1:920526/100‑1 (MQ=255)
                                                              gTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGAc                                >  1:3990322/1‑100 (MQ=255)
                                                                ctcCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCa                              >  2:3853609/1‑100 (MQ=255)
                                                                ctcCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCa                              >  1:885749/1‑100 (MQ=255)
                                                                ctcCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCa                              <  1:2020352/100‑1 (MQ=255)
                                                                ctcCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGAGCCATTGACGCCCTCAGTGACCa                              >  1:2976643/1‑100 (MQ=255)
                                                                 nccGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTCTTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAg                             >  2:3587575/2‑100 (MQ=255)
                                                                  ccGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGa                            >  1:1249891/1‑100 (MQ=255)
                                                                  ccGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGa                            >  2:4092865/1‑100 (MQ=255)
                                                                     gTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTAGCAGACCACCATCACCCGTAGCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGc                         >  2:2270059/1‑100 (MQ=255)
                                                                      ttttATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAATGACCAGATGc                         <  2:2186539/99‑1 (MQ=255)
                                                                        ttATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCtca                      <  2:3956158/100‑1 (MQ=255)
                                                                           tctatcAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCtcatca                   >  1:3174782/1‑100 (MQ=255)
                                                                           tctatcAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCtcatca                   >  2:66103/1‑100 (MQ=255)
                                                                            ctatcAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCAc                  >  2:2691633/1‑100 (MQ=255)
                                                                             tatcAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCAcc                 >  2:3123624/1‑100 (MQ=255)
                                                                             tatcAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCAcc                 >  2:867704/1‑100 (MQ=255)
                                                                             tatcAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCAATTGACGCCCTCAGTGACCAGATGCTCATCAcc                 >  2:1870918/1‑100 (MQ=255)
                                                                               tcAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGGCCAGATGCTCATCACCtt               >  1:2995461/1‑100 (MQ=255)
                                                                               tcAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCtt               >  1:2447498/1‑100 (MQ=255)
                                                                                cAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCttt              >  2:3153024/1‑100 (MQ=255)
                                                                                cAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCttt              >  1:399590/1‑100 (MQ=255)
                                                                                 aaTAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTc             >  2:3392635/1‑100 (MQ=255)
                                                                                 aaTAGAGGCTGAAACATGACCGTTATTTCTCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTc             <  2:1146066/100‑1 (MQ=255)
                                                                                   tAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGt           <  1:244868/100‑1 (MQ=255)
                                                                                   tAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGt           <  2:586843/100‑1 (MQ=255)
                                                                                   tAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGt           >  2:2028160/1‑100 (MQ=255)
                                                                                    agagGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTg          <  1:3462306/100‑1 (MQ=255)
                                                                                      agGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGaa        <  1:1270441/100‑1 (MQ=255)
                                                                                      agGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGaa        <  2:2469875/100‑1 (MQ=255)
                                                                                         cTGCAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCTCTCAGTGACCAGATGCTAATCACCTTTCGTGGAg       <  2:760754/98‑1 (MQ=255)
                                                                                         cTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGgc     >  1:4141744/1‑100 (MQ=255)
                                                                                           gAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGgcgc   >  1:1360919/1‑100 (MQ=255)
                                                                                            aaaCGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGgcgcg  >  2:1975691/1‑100 (MQ=255)
                                                                                                |                                                                                               
TCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCG  >  NC_000913/2827266‑2827457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A