Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F133 I0 R1 200 171.4 8351354 94.3% 7875326 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,105,396 A→T 22.8% L92Q (CTG→CAG)  cpxR ← response regulator in two‑component regulatory system with CpxA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,105,3960AT22.8% 323.4 / 94.4 161L92Q (CTG→CAG) cpxRresponse regulator in two‑component regulatory system with CpxA
Reads supporting (aligned to +/- strand):  ref base A (43/81);  new base T (14/23);  total (57/104)
Fisher's exact test for biased strand distribution p-value = 8.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 165 total aligned reads displayed.

TGCTGTTGCTCGCTCCAGTGCGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAATGTGTCGATAC  >  NC_000913/4105297‑4105495
                                                                                                   |                                                                                                   
tGCTGTTGCTCGCTCCAGTGCGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCa                                                                                                     <  2:1798839/100‑1 (MQ=255)
tGCTGTTGCTCGCTCCAGTGCGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCa                                                                                                     >  1:4075920/1‑100 (MQ=255)
  cTGTTGCTCGCTCCAGTGCGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCTGc                                                                                                   <  2:1203224/100‑1 (MQ=255)
  cTGTTGCTCGCTCCAGTGCGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGc                                                                                                   <  2:1375046/100‑1 (MQ=255)
   tGTTGCTCGCTCCAGTGCGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCt                                                                                                  >  2:2013972/1‑100 (MQ=255)
   tGTTGCTCGCTCCAGTGCGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCt                                                                                                  <  2:234901/100‑1 (MQ=255)
    gTTGCTCGCTCCAGTGCGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTc                                                                                                 <  2:1983236/100‑1 (MQ=255)
     ttGCTCGCTCCAGTGCGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCTGCTCa                                                                                                <  2:3064765/100‑1 (MQ=255)
     ttGCTCGCTCCAGTGCGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCTGCTCa                                                                                                <  1:694949/100‑1 (MQ=255)
      tGCTCGCTCCAGTGCGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAACCGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCaa                                                                                               >  2:178747/1‑100 (MQ=255)
       gctcgctcCAGTGCGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAg                                                                                              >  2:110990/1‑100 (MQ=255)
        ctcgctcCGGTGCGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAgg                                                                                             <  1:1825093/100‑1 (MQ=255)
        ctcgctcCAGTGCGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAgg                                                                                             <  1:1764606/100‑1 (MQ=255)
         tcgctcCAGTGCGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCTGCTCAAGGc                                                                                            >  1:42794/1‑100 (MQ=255)
         tcgctcCAGTGCGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGc                                                                                            <  1:2652679/100‑1 (MQ=255)
           gctcAAGTGCGAACGGCGCATGATCGTGCCAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGTGCCCAGCTCAATGCCg                                                                                          <  2:2056414/100‑1 (MQ=255)
            ctcCAGTGCGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCTGCTCAAGGCCga                                                                                         <  2:748138/100‑1 (MQ=255)
            ctcCAGTGCGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCga                                                                                         <  1:1697519/100‑1 (MQ=255)
              ccAGTGCGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCgaga                                                                                       <  2:1677095/100‑1 (MQ=255)
                   gcgcACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAAcgcg                                                                                  >  1:570314/1‑100 (MQ=255)
                   gCGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAAcgcg                                                                                  <  1:2165771/100‑1 (MQ=255)
                    cGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGa                                                                                 >  2:2653158/1‑100 (MQ=255)
                     gAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCTGCTCAAGGCCGAGAACGCGAt                                                                                >  1:3511666/1‑100 (MQ=255)
                     gAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGAt                                                                                <  1:1033270/100‑1 (MQ=255)
                     gAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGAt                                                                                <  2:2352153/100‑1 (MQ=255)
                     gAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGAt                                                                                <  1:2569318/100‑1 (MQ=255)
                     gAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGAt                                                                                <  1:1174802/100‑1 (MQ=255)
                      aaCGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATc                                                                               <  1:3695769/100‑1 (MQ=255)
                       aCGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCa                                                                              <  2:4139750/100‑1 (MQ=255)
                         ggCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCTGCTCAAGGCCGAGAACGCGATCAAg                                                                            >  2:972409/1‑100 (MQ=255)
                          gcgcAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCTGCTCAAGGCCGAGAACGCGATCAAGt                                                                           <  1:1490130/100‑1 (MQ=255)
                          gcgcAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGt                                                                           <  2:2720116/100‑1 (MQ=255)
                           cgcAGGATCGGGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTGGGGAGATAGGAATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAg                                                                            <  2:197932/98‑1 (MQ=255)
                           cgcAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGtt                                                                          >  2:261096/1‑100 (MQ=255)
                           cgcAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGtt                                                                          >  2:1875116/1‑100 (MQ=255)
                            gcAGTATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGt                                                                           <  2:3757047/98‑1 (MQ=255)
                            gcAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCTGCTCAAGGCCGAGAACGCGATCCAGTTc                                                                         >  2:2712866/1‑100 (MQ=255)
                            gatctaTCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTc                                                                         <  2:3223371/95‑1 (MQ=255)
                             cAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAGCGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCa                                                                        <  2:399200/100‑1 (MQ=255)
                             cAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCa                                                                        <  1:2843397/100‑1 (MQ=255)
                             cAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCa                                                                        <  1:1444289/100‑1 (MQ=255)
                                 aTCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCagatc                                                                    >  1:3223392/1‑96 (MQ=255)
                                 aTCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGc                                                                    >  1:3149449/1‑100 (MQ=255)
                                 aTCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGc                                                                    <  1:3193081/100‑1 (MQ=255)
                                   cgcgcgAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCTGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCcg                                                                  >  2:3871549/1‑100 (MQ=255)
                                         aaTACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCTGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGcc                                                            <  1:3289059/100‑1 (MQ=255)
                                             cGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCa                                                        <  1:878033/100‑1 (MQ=255)
                                             cGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCa                                                        >  2:3366647/1‑100 (MQ=255)
                                               tGCCACCCGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCTGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAAc                                                      <  2:2053859/100‑1 (MQ=255)
                                               tGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCTGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAAc                                                      <  1:4077287/100‑1 (MQ=255)
                                               tGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAAc                                                      >  2:3091140/1‑100 (MQ=255)
                                                 ccaccaGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCTGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACAt                                                    >  2:3587487/1‑100 (MQ=255)
                                                 ccaccaGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCTGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACAt                                                    <  2:1347052/100‑1 (MQ=255)
                                                 ccaccaGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACAt                                                    <  1:585713/100‑1 (MQ=255)
                                                  caccaGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCTGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATa                                                   <  1:1155068/100‑1 (MQ=255)
                                                   accaGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCTGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATaa                                                  >  2:2966186/1‑100 (MQ=255)
                                                   accaGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATaa                                                  >  1:3942559/1‑100 (MQ=255)
                                                   accaGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATaa                                                  >  2:1993395/1‑100 (MQ=255)
                                                   accaGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATaa                                                  >  2:2116168/1‑100 (MQ=255)
                                                    ccaGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAAt                                                 <  2:3204998/100‑1 (MQ=255)
                                                    ccaGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAAt                                                 >  1:104122/1‑100 (MQ=255)
                                                    ccaGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAAt                                                 <  1:3091158/100‑1 (MQ=255)
                                                      aGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGa                                               <  1:2193201/100‑1 (MQ=255)
                                                          cACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAgg                                           <  1:2013982/100‑1 (MQ=255)
                                                            cGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCg                                         >  2:365843/1‑100 (MQ=255)
                                                            cGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCg                                         >  2:579129/1‑100 (MQ=255)
                                                               tCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCt                                      >  1:1766480/1‑100 (MQ=255)
                                                                   tAAACGGTTTCGGGAGATAGTCATCTGCGCCCTGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGgtg                                  <  2:1090748/100‑1 (MQ=255)
                                                                   tAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGgtg                                  <  1:819839/100‑1 (MQ=255)
                                                                   tAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGgtg                                  <  2:3023476/100‑1 (MQ=255)
                                                                    aaaCGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGgtgt                                 <  2:2603192/100‑1 (MQ=255)
                                                                     aaCGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGgtgtg                                <  2:881888/100‑1 (MQ=255)
                                                                       cGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTc                              <  1:3069175/100‑1 (MQ=255)
                                                                         gTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCAAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTg                            <  2:651244/100‑1 (MQ=255)
                                                                            tCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCg                         <  2:564857/100‑1 (MQ=255)
                                                                             cGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGa                        <  2:1553047/100‑1 (MQ=255)
                                                                               ggAGATAGTCATCTGCGCCCTGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAg                      <  1:1635045/100‑1 (MQ=255)
                                                                               ggAGATAGTCATCTGCGCCCAGCTCAAGGCCGATAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAg                      <  1:975976/100‑1 (MQ=255)
                                                                               ggAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAg                      <  1:1110274/100‑1 (MQ=255)
                                                                               ggAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAg                      <  1:650568/100‑1 (MQ=255)
                                                                                 agaTAGTCATCTGCGCCCTGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTg                    <  2:3539848/100‑1 (MQ=255)
                                                                                  gaTAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGc                   <  1:339366/100‑1 (MQ=255)
                                                                                     aGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCttt                <  1:1993405/100‑1 (MQ=255)
                                                                                      gTCATCTGCGCCCTGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCtttt               <  2:3521573/100‑1 (MQ=255)
                                                                                      gTCATCTGCGCCCTGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACAGAATGACAGGCGCCTGGTGTGTCTGGCGAAGTGc                   >  2:250646/1‑96 (MQ=255)
                                                                                       tCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTa              >  1:3438470/1‑100 (MQ=255)
                                                                                         aTCTGCGCCCTGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCGGGTGTGTCTGGCGAAGTGCTTTTAAt            <  1:2540424/100‑1 (MQ=255)
                                                                                         aTCTGCGCCCAGCTCAAGGCCGAGAACGTGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAAt            <  1:1528633/100‑1 (MQ=255)
                                                                                         aTCTGCGCCCAGCTCAAGCCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTaa             <  2:4072315/99‑1 (MQ=255)
                                                                                          tCTGCGCCCTGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAAtg           <  2:42793/100‑1 (MQ=255)
                                                                                          cccgcgaacAGCTCAGGGCCGAGTACGCGATCAAGTTCACTGCCGCGCCCCGTCAACATAATGACAGGCGTCTGGTGTGTCTCGCGAAGTGCTTTTAAt            <  1:3750519/91‑1 (MQ=255)
                                                                                            tGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAAtgtg         <  1:711587/100‑1 (MQ=255)
                                                                                            tGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAAtgtg         <  1:416871/100‑1 (MQ=255)
                                                                                              cgcCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAATGTGTc       <  2:1289505/100‑1 (MQ=255)
                                                                                              cgcCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAATGTGTc       <  1:4117166/100‑1 (MQ=255)
                                                                                               gcCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAATGTGTCg      <  2:3339115/100‑1 (MQ=255)
                                                                                                cccAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAATGTGTCGa     >  1:2324945/1‑100 (MQ=255)
                                                                                                 ccAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAATGTGTCGAt    >  1:2963389/1‑100 (MQ=255)
                                                                                                   aGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAATGTGTCGATAc  >  1:2994133/1‑100 (MQ=255)
                                                                                                   aGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAATGTGTCGATAc  <  1:110992/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
TGCTGTTGCTCGCTCCAGTGCGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAATGTGTCGATAC  >  NC_000913/4105297‑4105495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A