Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F133 I0 R1 200 171.4 8351354 94.3% 7875326 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,323,955 T→G 78.1% N133T (AAC→ACC)  phnD ← phosphonate ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,323,9550TG78.1% 383.3 / 101.4 169N133T (AAC→ACC) phnDphosphonate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (19/18);  new base G (72/60);  total (91/78)
Fisher's exact test for biased strand distribution p-value = 8.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

Only 100 of 183 total aligned reads displayed.

CGAAGACGTAGTAGCCGGGGACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGAT  >  NC_000913/4323857‑4324054
                                                                                                  |                                                                                                   
 gAAGACGTAGTAGCCGGGGACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGgttg                                                                                                   >  2:489449/1‑100 (MQ=255)
  aaGACGTAGTAGCCGGGGACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGggtgt                                                                                                  <  1:1515258/100‑1 (MQ=255)
   aGACGTAGTAGCCGGGGACGAGGAAGCCCGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGggtgtt                                                                                                 <  1:3961771/100‑1 (MQ=255)
   aGACGTAGTAGCCGGGGACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGgttgtt                                                                                                 <  2:4044820/100‑1 (MQ=255)
    ggcGTAGCATCCGGCGATGGGGAAGCGAGAGGCGGCGTGAGGATCGCCATTGCCAAAGGTGGGATTTTTCCGCTGCGCCAGCAGATCGTTCAGggtgt                                                                                                  <  2:3787226/96‑1 (MQ=255)
    gACGTAGTAGCCGGGGACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGgtgttg                                                                                                <  2:3031774/100‑1 (MQ=255)
      cGTAGTAGCCGGGGACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGAt                                                                                              >  2:3452343/1‑100 (MQ=255)
       gtagtaGCCGGGGACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATc                                                                                             >  1:2631532/1‑100 (MQ=255)
        tagtagCCGGGGACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCg                                                                                            <  2:106325/100‑1 (MQ=255)
        tagtagCCGGGGACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCg                                                                                            <  1:4146898/100‑1 (MQ=255)
        tagtagCCGGGGACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCg                                                                                            <  2:1617531/100‑1 (MQ=255)
         agtagCCGGGGACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCgg                                                                                           <  1:2864300/100‑1 (MQ=255)
         agtagCCGGGGACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCgg                                                                                           >  1:2453779/1‑100 (MQ=255)
          gtagCCGGTGACGAGGAAGCCAGAGCTGGAGTTACGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGa                                                                                          <  2:234325/100‑1 (MQ=255)
          gtagCCGGGGACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGa                                                                                          >  2:2207957/1‑100 (MQ=255)
           tagCCGGGGACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGAc                                                                                         <  2:3879680/100‑1 (MQ=255)
             gCCGGGGACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTg                                                                                       <  1:2118191/100‑1 (MQ=255)
             gCCGGGGACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTACGCCAGCAGATCGTTCAGGGTGTTGATCGGACTg                                                                                       >  2:2197884/1‑100 (MQ=255)
                ggggACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCt                                                                                    >  2:345482/1‑100 (MQ=255)
                ggggACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTACAGGGTGTTGATCGGACTGt                                                                                      >  2:1433216/1‑98 (MQ=255)
                 gggACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCtt                                                                                   <  1:1209725/100‑1 (MQ=255)
                    aCGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTttgt                                                                                <  1:2364620/100‑1 (MQ=255)
                     cGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTttgtt                                                                               <  2:2631517/100‑1 (MQ=255)
                     cGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTttgtt                                                                               <  1:2638645/100‑1 (MQ=255)
                     cGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTttgtt                                                                               <  2:3418900/100‑1 (MQ=255)
                      gAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTttgttg                                                                              <  1:1980858/100‑1 (MQ=255)
                       aGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGa                                                                             <  1:2207971/100‑1 (MQ=255)
                        gtacggcggaggtgaggtTGGGATCGCCATGGCCAATGTGGTGGTCTTTCCGCTTCGACCACAGATCGTTCAGGGTGTTGATCTGGCTGTCTTTGTTGAc                                                                            <  2:4001373/92‑1 (MQ=255)
                        ggAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGAc                                                                            >  1:607155/1‑100 (MQ=255)
                        ggAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGAc                                                                            <  1:3438942/100‑1 (MQ=255)
                        ggAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGAc                                                                            >  2:2136951/1‑100 (MQ=255)
                         gAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCTTTCAGGTTGTTGATCGGACTGTCTTTGTTGACg                                                                           <  2:1145521/100‑1 (MQ=255)
                          aaGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGa                                                                          <  1:2720380/100‑1 (MQ=255)
                           aGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGAt                                                                         >  1:733083/1‑100 (MQ=255)
                           aGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGAt                                                                         <  1:1310440/100‑1 (MQ=255)
                            gCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCTTTCAGGTTGTTGATCGGACTGTCTTTGTTGAc                                                                            <  2:3250359/96‑1 (MQ=255)
                             ccAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCa                                                                       >  1:1399564/1‑100 (MQ=255)
                             ccAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGCGTGTTGATCGGACTGTCTTTGTTGACGATCa                                                                       >  2:531408/1‑100 (MQ=255)
                              cAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCaa                                                                      >  1:3964882/1‑100 (MQ=255)
                               agagGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAac                                                                     <  1:540658/100‑1 (MQ=255)
                                  ggTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACg                                                                  >  1:3082010/1‑100 (MQ=255)
                                  ggTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAacac                                                                   >  2:667268/1‑99 (MQ=255)
                                  ggTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACg                                                                  >  1:1872347/1‑100 (MQ=255)
                                  ggTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACg                                                                  >  2:3599349/1‑100 (MQ=255)
                                  ggTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACg                                                                  <  1:3272893/100‑1 (MQ=255)
                                     ggAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTc                                                               >  1:853294/1‑100 (MQ=255)
                                      gAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTcc                                                              <  1:452978/100‑1 (MQ=255)
                                      gAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTcc                                                              <  2:442526/100‑1 (MQ=255)
                                      gAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTcc                                                              <  1:1320322/100‑1 (MQ=255)
                                      gAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTcc                                                              <  1:1701657/100‑1 (MQ=255)
                                        gTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAg                                                            >  1:4072062/1‑100 (MQ=255)
                                          tAGGGTCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTa                                                          <  2:1574850/100‑1 (MQ=255)
                                          tAGGATCGCCATTGCCAAAGGTGAGATCTTTCAGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTa                                                          <  1:3746316/100‑1 (MQ=255)
                                          gaGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTa                                                          >  2:3959047/2‑100 (MQ=255)
                                             gATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAAcc                                                       >  2:144021/1‑100 (MQ=255)
                                             gATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAAcc                                                       >  1:3489597/1‑100 (MQ=255)
                                              aTCGCCATTGCCAAAGGTGAGATCTTTCCGCTTAGCCAGTAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCATTAAccc                                                      <  2:1024877/100‑1 (MQ=255)
                                                cGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCgg                                                    >  1:3158746/1‑100 (MQ=255)
                                                 gCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGc                                                   >  2:3152335/1‑100 (MQ=255)
                                                 gCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGc                                                   <  2:890439/100‑1 (MQ=255)
                                                 gCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGc                                                   >  2:3825984/1‑100 (MQ=255)
                                                 gCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGc                                                   <  1:3136848/100‑1 (MQ=255)
                                                 gCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGc                                                   <  1:1900379/100‑1 (MQ=255)
                                                   cATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCTGGCTGTCCGCGTTGACAATAAACACGCTACAGTAACCCcgcg                                                  >  2:1534471/1‑99 (MQ=255)
                                                     ttGGCAAAGGTGATTTCTTTCCGCTACGCCAGCAGATCGTTCAGGTTTTTTATTGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGc                                                   <  2:193630/96‑1 (MQ=255)
                                                       gCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGatcca                                             >  2:1104774/1‑100 (MQ=255)
                                                        ccAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGatccat                                            <  1:2341883/100‑1 (MQ=255)
                                                         cAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGatccatc                                           <  1:1291305/100‑1 (MQ=255)
                                                                gagaTCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACAAGCTCCAGTAACCCGGCGATCCATCCGCcgcg                                    >  2:798077/1‑100 (MQ=255)
                                                                 agaTCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGa                                   >  1:3805427/1‑100 (MQ=255)
                                                                  gaTCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGAc                                  >  1:1050603/1‑100 (MQ=255)
                                                                  gaTCTTTCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGAc                                  >  1:3187344/1‑100 (MQ=255)
                                                                   aTCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTCTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGAc                                  <  2:2829902/99‑1 (MQ=255)
                                                                      tttCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTc                              >  1:791607/1‑100 (MQ=255)
                                                                      tttCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTc                              >  2:2563354/1‑100 (MQ=255)
                                                                       ttCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCt                             <  1:1439996/100‑1 (MQ=255)
                                                                       ttCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCt                             >  1:2510756/1‑100 (MQ=255)
                                                                        tCCGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTg                            >  1:622218/1‑100 (MQ=255)
                                                                         ccGCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTgg                           <  1:542515/100‑1 (MQ=255)
                                                                          cGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGAGTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTggg                          >  1:2941716/1‑100 (MQ=255)
                                                                           gCTTCGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTTTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGc                         <  2:2667448/100‑1 (MQ=255)
                                                                               cGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAg                     <  1:3340956/100‑1 (MQ=255)
                                                                               cGCCAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAg                     >  2:3538518/1‑100 (MQ=255)
                                                                                 ccAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGAc                   <  2:2832026/100‑1 (MQ=255)
                                                                                 ccAGCAGATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGAc                   >  2:2006381/1‑100 (MQ=255)
                                                                                   agcagATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCt                 <  1:1080058/100‑1 (MQ=255)
                                                                                   agcagATCGTTCAGGGTGTTGATCGGACTGTCTTCGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCt                 >  2:38491/1‑100 (MQ=255)
                                                                                      agATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGc              >  2:1696312/1‑100 (MQ=255)
                                                                                       gATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGcc             >  2:358154/1‑100 (MQ=255)
                                                                                       gATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGcc             >  1:3917797/1‑100 (MQ=255)
                                                                                       gATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGcc             >  1:475092/1‑100 (MQ=255)
                                                                                       gATCGTTCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGcc             >  1:762432/1‑100 (MQ=255)
                                                                                          cGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCAtt          >  2:1380507/1‑100 (MQ=255)
                                                                                           gTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTg         >  1:3578621/1‑100 (MQ=255)
                                                                                            ttCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTgg        <  1:2068998/100‑1 (MQ=255)
                                                                                            ttCAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCAtt          >  1:223347/1‑98 (MQ=255)
                                                                                              cAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGACGACCTGGCCATTGgcg      >  1:1574606/1‑100 (MQ=255)
                                                                                              cAGGGTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGgcg      <  1:1036387/100‑1 (MQ=255)
                                                                                                  ttgttgATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGAt  <  1:36274/100‑1 (MQ=255)
                                                                                                  ttgttgATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGAt  <  1:96784/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
CGAAGACGTAGTAGCCGGGGACGAGGAAGCCAGAGGTGGAGTTAGGATCGCCATTGCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTGGCGCGAT  >  NC_000913/4323857‑4324054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A