Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F56 I1 R1
|
165 |
53.8 |
2611523 |
96.4% |
2517508 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,892,993 |
G→A |
E113K (GAA→AAA) |
ygcN → |
putative oxidoreductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,892,993 | 0 | G | A | 90.9%
| 127.5
/ 3.6
| 45 | E113K (GAA→AAA) | ygcN | putative oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (3/1); new base A (18/22); total (22/23) |
| Fisher's exact test for biased strand distribution p-value = 3.35e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTGTGGTGACGAT > NC_000913/2892898‑2893085
|
ccGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAaaaa < 2:582295/100‑1 (MQ=255)
cGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCaaaaaa < 1:73967/100‑1 (MQ=255)
ggCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAaagaag < 1:203188/100‑1 (MQ=255)
ggCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAaagaag < 1:83498/100‑1 (MQ=255)
ggCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAaagaag < 1:543652/100‑1 (MQ=255)
gCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAgg < 1:802748/100‑1 (MQ=255)
acgacgTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCg < 2:839415/100‑1 (MQ=255)
acgacgTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCg < 2:1150020/100‑1 (MQ=255)
cgacgTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGa > 2:79323/1‑100 (MQ=255)
acgTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGaa < 2:39513/99‑1 (MQ=255)
cgTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATg > 2:633094/1‑100 (MQ=255)
cgTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATg > 2:632079/1‑100 (MQ=255)
cgTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATg < 1:1005684/100‑1 (MQ=255)
cgTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATg < 1:764057/100‑1 (MQ=255)
gTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGc > 2:292029/1‑100 (MQ=255)
ttACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAg > 2:999159/1‑100 (MQ=255)
tACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGc < 1:478802/100‑1 (MQ=255)
tACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGc > 1:483864/1‑100 (MQ=255)
cAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGa > 2:1176884/1‑100 (MQ=255)
aGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGAc < 1:1244377/100‑1 (MQ=255)
gCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACg > 1:966804/1‑100 (MQ=255)
ggcgtTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTgg < 2:346870/100‑1 (MQ=255)
gTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGc < 2:305739/100‑1 (MQ=255)
gTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGc < 2:483860/100‑1 (MQ=255)
aTCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCCAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTg > 1:132134/1‑100 (MQ=255)
ccTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTa > 2:1285414/1‑100 (MQ=255)
cTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTAt < 2:665882/100‑1 (MQ=255)
cTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTAt > 2:521412/1‑100 (MQ=255)
cTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGGTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTAt > 1:665156/1‑100 (MQ=255)
aGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATgaag > 2:264885/1‑100 (MQ=255)
aGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATgaag < 2:723595/100‑1 (MQ=255)
gtAATACGTGCACGTTTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATgaagaa < 2:221222/99‑1 (MQ=255)
tACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACgg > 1:343902/1‑100 (MQ=255)
aCGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGc > 1:844809/1‑100 (MQ=255)
aCGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGc < 2:217055/100‑1 (MQ=255)
gCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCagag < 1:958854/100‑1 (MQ=255)
gATTCGTTCCGTGGCTGGTTGCCGAAGGCGAAAAAGAAGGTGGCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTc < 2:431171/98‑1 (MQ=255)
aTTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCtgt > 1:516606/1‑100 (MQ=255)
cGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCtgtgac > 1:382076/1‑98 (MQ=255)
gATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCg > 1:581359/1‑100 (MQ=255)
tCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGtt < 1:959787/100‑1 (MQ=255)
ggCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTtg > 1:212951/1‑100 (MQ=255)
tGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTgtgg < 2:599991/100‑1 (MQ=255)
ggTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTgtggt > 1:124946/1‑100 (MQ=255)
gTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCG‑TATTTGTGGTGa > 1:509966/1‑100 (MQ=255)
tGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTGTGGTGAc > 2:1172033/1‑100 (MQ=255)
gccgaagccaaaAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTGTGGTGACg > 2:676958/1‑100 (MQ=255)
ccgaagccaaaAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTGTGGTGACGa < 1:800615/100‑1 (MQ=255)
ccgaagccaaaAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTGTGGTGACGa > 2:840486/1‑100 (MQ=255)
cgaagccaaaAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTGTGGTGACGat < 1:292032/100‑1 (MQ=255)
|
CCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTGTGGTGACGAT > NC_000913/2892898‑2893085
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
