Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F56 I1 R1
|
165 |
53.8 |
2611523 |
96.4% |
2517508 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,379,768 |
+C |
coding (230/1128 nt) |
zapE ← |
divisome ATPase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,379,764 | 1 | . | C | 96.7%
| 99.4
/ ‑3.1
| 30 | coding (234/1128 nt) | zapE | divisome ATPase |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (15/14); total (16/14) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑CCC‑CACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCT > NC_000913/3379665‑3379856
|
ccGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCg < 2:1134400/100‑1 (MQ=255)
cagcaTAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑ccc < 2:1029624/100‑1 (MQ=255)
caTAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGTCAGGTTTTCCCGCGTCCTACACCG‑ccc‑cca < 2:146649/100‑3 (MQ=255)
caTAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑ccc‑cca > 2:1007396/1‑98 (MQ=255)
tAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑ccc‑ccaca > 2:955093/1‑96 (MQ=255)
aaaaCGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCTGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑ccc‑ccacat > 2:1122484/1‑95 (MQ=255)
aaaCGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCC‑CACata > 1:234292/1‑100 (MQ=255)
cGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCC‑CACatatat > 1:1184124/1‑100 (MQ=255)
taaaaGTGCAGGCACTGTTTCCGCTCTCCCGACAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCC‑CACATATATAAg > 1:878218/3‑100 (MQ=255)
tGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCC‑CACATATATAAg < 2:921035/100‑1 (MQ=255)
gTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCC‑CACATATATAAGCCACg > 2:750051/1‑100 (MQ=255)
cTGTTTCCGCTCTTCCGGCAGGCTTTGATTGAAAAGGTCCATCAGCCAGGTTTTCCTGTGTTCTACATTT‑CCCTCACATATATAAGCCACGCACTGGc > 2:868840/1‑98 (MQ=255)
tggttCCGCACTCCCGGCAGGCCTTGTTAGAAAAGCGCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCC‑CACATATATAAGCCACGCGCTGGCGTa < 2:936567/97‑1 (MQ=255)
gTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCC‑CACATATATAAGCCACGCACTGGCGTAt < 1:325988/100‑1 (MQ=255)
gTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCC‑CACATATATAAGCCACGCACTGGCGTAt < 2:1060725/100‑1 (MQ=255)
tCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCC‑CACATATATAAGCCACGCACTGGCGTATGCt > 2:870944/1‑100 (MQ=255)
cGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCC‑CACATATATAAGCCACGCACTGGCGTATGCttt < 2:307645/100‑1 (MQ=255)
gCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCC‑CACATATATAAGCCACGCACTGGCGTATGCTTtg < 2:667986/100‑1 (MQ=255)
ctcCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCC‑CACATATATAAGCCACGCACTGGCGTATGCTTtgtg < 1:402765/99‑1 (MQ=255)
ccGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCC‑CACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCtt > 1:538671/1‑100 (MQ=255)
ccGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCC‑CACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCtt > 1:538249/1‑100 (MQ=255)
ggCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCC‑CACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTcg > 1:525433/1‑100 (MQ=255)
ggCTTTGATAGAAAAGGTCCATCAGCCAGGTTATCCCGCCTCATACACCGCCCC‑CACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGtt < 2:914996/100‑1 (MQ=255)
gCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCC‑CACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGttt > 2:279459/1‑100 (MQ=255)
tGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCC‑CACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTAccc > 2:442113/1‑100 (MQ=255)
ccATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCC‑CACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGa > 1:554833/1‑100 (MQ=255)
aTCAGCCAGGTTTTCCCGCGTCCTACACCGCCCC‑CACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGAc > 2:423278/1‑99 (MQ=255)
gCCAGGTTTTCCCGCGTCCTACACCGCCCC‑CACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCcgcg > 1:1058749/1‑100 (MQ=255)
ccAGGTTTTCCCGCGTCCTACACCGCCCC‑CACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCcgcn < 2:480538/99‑2 (MQ=255)
tttCCCGCGTCCTACACCGCCCC‑CACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAg < 1:870950/100‑1 (MQ=255)
tCCCGCGTCCTACACCGCCCC‑CACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGcc < 2:328857/100‑1 (MQ=255)
cccGCGTCCTACACCGCCCC‑CACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGccc > 1:156462/1‑100 (MQ=255)
cccGCGTCCTACACCGCCCC‑CACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGccc < 1:868846/100‑1 (MQ=255)
cgTCCTACACCGCCCC‑CACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTc < 1:955101/100‑1 (MQ=255)
ccTACACCGCCCC‑CACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTc < 2:435325/100‑1 (MQ=255)
tACACCGCCCC‑CACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCt > 2:76438/1‑100 (MQ=255)
|
CCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑CCC‑CACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCT > NC_000913/3379665‑3379856
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
