Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F56 I1 R1
|
165 |
53.8 |
2611523 |
96.4% |
2517508 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,631,700 |
T→C |
L323P (CTT→CCT) |
slt → |
lytic murein transglycosylase, soluble |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,631,700 | 0 | T | C | 96.0%
| 168.9
/ ‑3.5
| 50 | L323P (CTT→CCT) | slt | lytic murein transglycosylase, soluble |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (18/30); total (19/31) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGAT > NC_000913/4631603‑4631794
|
ggCAACGATGTCACCGACGAGCAGGTGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTg < 2:820841/100‑1 (MQ=255)
cGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCAcc < 2:1060798/100‑1 (MQ=255)
tCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGa < 1:210076/100‑1 (MQ=255)
gAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAAt < 2:510723/100‑1 (MQ=255)
ggCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACctg < 1:606213/100‑1 (MQ=255)
gcgATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACctggct > 2:1232954/1‑100 (MQ=255)
gATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACctggctgg < 1:936495/100‑1 (MQ=255)
tGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGgcg < 1:1256301/100‑1 (MQ=255)
tGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGgcg < 1:310290/100‑1 (MQ=255)
naCGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGgcgc > 2:651218/2‑100 (MQ=255)
gACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGgcgc > 2:686870/1‑100 (MQ=255)
cGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGcgcg > 1:446722/1‑99 (MQ=255)
gCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTc < 2:1249876/100‑1 (MQ=255)
gCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTc < 2:621420/100‑1 (MQ=255)
cATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTg < 1:556775/100‑1 (MQ=255)
aTTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGc < 1:277780/100‑1 (MQ=255)
tATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCt > 2:386069/1‑100 (MQ=255)
tATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCt < 2:1075739/100‑1 (MQ=255)
gcgcTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATg < 2:1093362/100‑1 (MQ=255)
cgcTCGCAATCTACTTCGCTTATTGAACGCCGCGTACGACTGACGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATgg < 2:914359/100‑1 (MQ=255)
gcTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGa < 1:365179/100‑1 (MQ=255)
gCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCg < 1:1189057/100‑1 (MQ=255)
cAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCga < 1:1232965/100‑1 (MQ=255)
cAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCga > 2:1031333/1‑100 (MQ=255)
cAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCga < 2:275154/100‑1 (MQ=255)
aTCTACTTCGCTTATTTAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaa > 1:639309/1‑100 (MQ=255)
aTCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaa > 2:776073/1‑100 (MQ=255)
tCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaag < 2:661731/100‑1 (MQ=255)
cTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGTCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaaga > 1:281181/1‑100 (MQ=255)
cTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaaga > 1:669732/1‑100 (MQ=255)
tACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagag > 1:807551/1‑100 (MQ=255)
aCTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagaga < 1:255634/100‑1 (MQ=255)
cTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagagaa > 1:974191/1‑100 (MQ=255)
ttCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagagaaa < 1:843457/100‑1 (MQ=255)
cTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATg < 1:560015/100‑1 (MQ=255)
cTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATg < 1:386073/100‑1 (MQ=255)
cTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATg > 1:241457/1‑100 (MQ=255)
ttATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGa > 2:197793/1‑100 (MQ=255)
gAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGc < 2:773619/100‑1 (MQ=255)
aaCGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCg < 1:1260271/100‑1 (MQ=255)
aCGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGt > 1:896604/1‑100 (MQ=255)
aCGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGt > 2:18249/1‑100 (MQ=255)
cGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGtt < 2:600207/100‑1 (MQ=255)
gtACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGc < 1:1043725/100‑1 (MQ=255)
cggagggCGCTTGGCGCCGGCGATCATCGCGTCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAgg < 2:1294220/95‑1 (MQ=255)
cGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAgg > 1:170953/1‑100 (MQ=255)
aaTGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCg > 1:24734/1‑100 (MQ=255)
ggCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGAt < 2:362654/100‑1 (MQ=255)
ggCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGAt < 2:362618/100‑1 (MQ=255)
ggCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGAt > 2:868139/1‑100 (MQ=255)
|
GGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGAT > NC_000913/4631603‑4631794
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
