Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F204 I1 R1
|
117 |
93.2 |
4682243 |
93.4% |
4373214 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,686,092 |
G→T |
T166N (ACC→AAC) |
fumC ← |
fumarate hydratase (fumarase C),aerobic Class II |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,686,092 | 0 | G | T | 97.3%
| 256.8
/ ‑0.9
| 74 | T166N (ACC→AAC) | fumC | fumarate hydratase (fumarase C),aerobic Class II |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (33/39); total (34/40) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 |
CCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGTTC > NC_000913/1685995‑1686187
|
ncccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGttt > 1:2044852/2‑100 (MQ=255)
ccccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGttt > 1:598583/1‑100 (MQ=255)
cccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGtttt < 1:2252014/100‑1 (MQ=255)
ccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTtttt > 1:159597/1‑100 (MQ=255)
ccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTtttt > 1:1600605/1‑100 (MQ=255)
cAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTa < 2:495278/100‑1 (MQ=255)
cGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGc > 1:1758183/1‑100 (MQ=255)
cGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAGc < 1:2117248/100‑1 (MQ=255)
cGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGACAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGc < 2:147090/100‑1 (MQ=255)
gTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCt < 2:461253/100‑1 (MQ=255)
tAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGa > 2:2124554/1‑100 (MQ=255)
aCGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAgg < 1:2211554/100‑1 (MQ=255)
aCGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAgg < 2:964657/100‑1 (MQ=255)
cGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGa > 1:2059765/1‑100 (MQ=255)
ggCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGaa < 2:1468735/100‑1 (MQ=255)
gTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGAGTCTGTGTGAGGTTTTTAAGCTGAGGAATGa > 2:1578837/1‑100 (MQ=255)
gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGtt < 1:419090/100‑1 (MQ=255)
cATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGttg > 2:903036/1‑100 (MQ=255)
aTCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGttgc < 1:44671/100‑1 (MQ=255)
ccTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGttgctt > 1:2012710/1‑100 (MQ=255)
ccTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGttgcgg < 2:266787/100‑3 (MQ=255)
cTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGttgcttg < 1:928777/100‑1 (MQ=255)
tGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGttgcttgc < 2:2002438/100‑1 (MQ=255)
tGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGttgcttgc > 1:1147487/1‑100 (MQ=255)
gCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTgcg < 2:1799947/100‑1 (MQ=255)
cAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTgcgc > 1:2140211/1‑100 (MQ=255)
cAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTgcgc > 1:2140115/1‑100 (MQ=255)
cAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTgcgc < 2:809523/100‑1 (MQ=255)
gTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAgc < 1:2100985/100‑1 (MQ=255)
aGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGcc < 2:455155/100‑1 (MQ=255)
gTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCca > 1:1423290/1‑100 (MQ=255)
cGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCcagca < 2:369077/100‑1 (MQ=255)
gACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCTgctggcgctg < 1:1310094/100‑9 (MQ=255)
aCCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCagcagc > 1:2019357/1‑100 (MQ=255)
aCCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCagcagc > 1:2021258/1‑100 (MQ=255)
aCCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCagcagc < 1:2209727/100‑1 (MQ=255)
cAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAgcgc < 1:2145078/100‑1 (MQ=255)
aaTTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGcgcc > 2:2112887/1‑100 (MQ=255)
ttGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCaa > 2:62552/1‑100 (MQ=255)
gACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACg > 1:785077/1‑100 (MQ=255)
cGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTg > 2:793103/1‑100 (MQ=255)
tatCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCAt > 1:677343/1‑100 (MQ=255)
atCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCAtn < 2:2044845/100‑2 (MQ=255)
tCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCg > 1:868656/1‑100 (MQ=255)
cGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGc > 1:1925515/1‑100 (MQ=255)
aaaaGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTc < 1:784654/100‑1 (MQ=255)
aaaaGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTc < 1:162198/100‑1 (MQ=255)
aaaGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCg < 1:2264895/100‑1 (MQ=255)
aaaGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCg < 2:2012703/100‑1 (MQ=255)
aaGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCgg > 2:1042954/1‑100 (MQ=255)
aGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGa < 2:1005720/100‑1 (MQ=255)
gCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGaa < 1:903038/100‑1 (MQ=255)
tccgatctctcATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGa < 1:1596045/93‑1 (MQ=255)
gggATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCCCAGCGCCAGCTGCGCCGCAACGTGCATCGCCGTCGGAAAgcc < 1:1578842/100‑3 (MQ=255)
gATTTCTCATTCAGTGTCTGTGCCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACAt > 2:1040568/1‑100 (MQ=255)
tctcATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGtt > 2:1451686/1‑100 (MQ=255)
ctcATTCAGTGTCTGTGTCAGGTTTTTTTTTTTTTTTTTTAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTg > 2:1618037/1‑100 (MQ=255)
ctcATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGaagatcgga > 2:1596040/1‑92 (MQ=255)
ctcATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTg > 1:1942577/1‑100 (MQ=255)
ctcATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTg < 2:1758178/100‑1 (MQ=255)
tcATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTgg < 2:2059758/100‑1 (MQ=255)
ttCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAAc < 1:793104/100‑1 (MQ=255)
aGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACttt < 2:598582/100‑1 (MQ=255)
gtgtCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTg < 2:1147485/100‑1 (MQ=255)
tgtCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTgg < 2:1717903/100‑1 (MQ=255)
gtCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGc < 1:480668/100‑1 (MQ=255)
gtCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCAGCGCCGTCGGAAAGACATCGTTGGAACTTTgg < 2:734788/99‑1 (MQ=255)
tCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt > 1:974136/1‑100 (MQ=255)
tgtgTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCttt > 2:26206/1‑100 (MQ=255)
tgtgTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCttt < 2:2140204/100‑1 (MQ=255)
tgtgTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCttt < 2:2140108/100‑1 (MQ=255)
tCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGtt > 1:384516/1‑100 (MQ=255)
tCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGtt > 2:128533/1‑100 (MQ=255)
cAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGTTc > 2:579538/1‑100 (MQ=255)
cAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGTTc > 2:2237910/1‑100 (MQ=255)
cAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGTTc < 2:868654/100‑1 (MQ=255)
aGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGTTc > 2:1539379/1‑99 (MQ=255)
|
CCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGTTC > NC_000913/1685995‑1686187
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
